Values CatLog read from this paper
Effects of the mutational combinations on the activity and stability of thermolysin
PMID 20214932doi:10.1016/j.jbiotec.2010.02.024Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 42 records (70 values) from this paper: thermolysin from Bacillus thermoproteolyticus.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| thermolysin | Bacillus thermoproteolyticus | N-[3-(2-furyl)acryloyl]-Gly-L-Leu amide0 M NaCl | — | — | 29 | pH 7.5, 25 °C | Open record: thermolysin, N-[3-(2-furyl)acryloyl]-Gly-L-Leu amide |
| thermolysin | Bacillus thermoproteolyticus | N-[3-(2-furyl)acryloyl]-Gly-L-Leu amide4 M NaCl | — | — | 380 | pH 7.5, 25 °C | Open record: thermolysin, N-[3-(2-furyl)acryloyl]-Gly-L-Leu amide |
| thermolysin | Bacillus thermoproteolyticus | N-carbobenzoxy-L-Asp-L-Phe methyl ester | 5.9 | 0.63 | 9.4 | pH 7.5, 25 °C | Open record: thermolysin, N-carbobenzoxy-L-Asp-L-Phe methyl ester |
| thermolysinvariant D150E | Bacillus thermoproteolyticus | N-[3-(2-furyl)acryloyl]-Gly-L-Leu amide0 M NaCl | — | — | 79 | pH 7.5, 25 °C | Open record: thermolysin variant D150E, N-[3-(2-furyl)acryloyl]-Gly-L-Leu amide |
| thermolysinvariant D150E | Bacillus thermoproteolyticus | N-[3-(2-furyl)acryloyl]-Gly-L-Leu amide4 M NaCl | — | — | 400 | pH 7.5, 25 °C | Open record: thermolysin variant D150E, N-[3-(2-furyl)acryloyl]-Gly-L-Leu amide |
| thermolysinvariant D150E | Bacillus thermoproteolyticus | N-carbobenzoxy-L-Asp-L-Phe methyl ester | 16.5 | 0.5 | 33.1 | pH 7.5, 25 °C | Open record: thermolysin variant D150E, N-carbobenzoxy-L-Asp-L-Phe methyl ester |
| thermolysinvariant D150E/I168A | Bacillus thermoproteolyticus | N-[3-(2-furyl)acryloyl]-Gly-L-Leu amide0 M NaCl | — | — | 141 | pH 7.5, 25 °C | Open record: thermolysin variant D150E/I168A, N-[3-(2-furyl)acryloyl]-Gly-L-Leu amide |
| thermolysinvariant D150E/I168A | Bacillus thermoproteolyticus | N-[3-(2-furyl)acryloyl]-Gly-L-Leu amide4 M NaCl | — | — | 600 | pH 7.5, 25 °C | Open record: thermolysin variant D150E/I168A, N-[3-(2-furyl)acryloyl]-Gly-L-Leu amide |
| thermolysinvariant D150E/I168A | Bacillus thermoproteolyticus | N-carbobenzoxy-L-Asp-L-Phe methyl ester | 15.5 | 0.18 | 83.8 | pH 7.5, 25 °C | Open record: thermolysin variant D150E/I168A, N-carbobenzoxy-L-Asp-L-Phe methyl ester |
| thermolysinvariant G8C/N60C/S65P | Bacillus thermoproteolyticus | N-[3-(2-furyl)acryloyl]-Gly-L-Leu amide0 M NaCl | — | — | 29 | pH 7.5, 25 °C | Open record: thermolysin variant G8C/N60C/S65P, N-[3-(2-furyl)acryloyl]-Gly-L-Leu amide |
| thermolysinvariant G8C/N60C/S65P | Bacillus thermoproteolyticus | N-[3-(2-furyl)acryloyl]-Gly-L-Leu amide4 M NaCl | — | — | 350 | pH 7.5, 25 °C | Open record: thermolysin variant G8C/N60C/S65P, N-[3-(2-furyl)acryloyl]-Gly-L-Leu amide |
| thermolysinvariant G8C/N60C/S65P | Bacillus thermoproteolyticus | N-carbobenzoxy-L-Asp-L-Phe methyl ester | 5.4 | 0.54 | 10 | pH 7.5, 25 °C | Open record: thermolysin variant G8C/N60C/S65P, N-carbobenzoxy-L-Asp-L-Phe methyl ester |
| thermolysinvariant I168A | Bacillus thermoproteolyticus | N-[3-(2-furyl)acryloyl]-Gly-L-Leu amide0 M NaCl | — | — | 62 | pH 7.5, 25 °C | Open record: thermolysin variant I168A, N-[3-(2-furyl)acryloyl]-Gly-L-Leu amide |
| thermolysinvariant I168A | Bacillus thermoproteolyticus | N-[3-(2-furyl)acryloyl]-Gly-L-Leu amide4 M NaCl | — | — | 300 | pH 7.5, 25 °C | Open record: thermolysin variant I168A, N-[3-(2-furyl)acryloyl]-Gly-L-Leu amide |
| thermolysinvariant I168A | Bacillus thermoproteolyticus | N-carbobenzoxy-L-Asp-L-Phe methyl ester | 7.7 | 0.46 | 16.8 | pH 7.5, 25 °C | Open record: thermolysin variant I168A, N-carbobenzoxy-L-Asp-L-Phe methyl ester |
| thermolysinvariant L144S | Bacillus thermoproteolyticus | N-[3-(2-furyl)acryloyl]-Gly-L-Leu amide0 M NaCl | — | — | 182 | pH 7.5, 25 °C | Open record: thermolysin variant L144S, N-[3-(2-furyl)acryloyl]-Gly-L-Leu amide |
| thermolysinvariant L144S | Bacillus thermoproteolyticus | N-[3-(2-furyl)acryloyl]-Gly-L-Leu amide4 M NaCl | — | — | 660 | pH 7.5, 25 °C | Open record: thermolysin variant L144S, N-[3-(2-furyl)acryloyl]-Gly-L-Leu amide |
| thermolysinvariant L144S | Bacillus thermoproteolyticus | N-carbobenzoxy-L-Asp-L-Phe methyl ester | 9.4 | 0.19 | 49.7 | pH 7.5, 25 °C | Open record: thermolysin variant L144S, N-carbobenzoxy-L-Asp-L-Phe methyl ester |
| thermolysinvariant L144S/D150E | Bacillus thermoproteolyticus | N-[3-(2-furyl)acryloyl]-Gly-L-Leu amide0 M NaCl | — | — | 204 | pH 7.5, 25 °C | Open record: thermolysin variant L144S/D150E, N-[3-(2-furyl)acryloyl]-Gly-L-Leu amide |
| thermolysinvariant L144S/D150E | Bacillus thermoproteolyticus | N-[3-(2-furyl)acryloyl]-Gly-L-Leu amide4 M NaCl | — | — | 590 | pH 7.5, 25 °C | Open record: thermolysin variant L144S/D150E, N-[3-(2-furyl)acryloyl]-Gly-L-Leu amide |
| thermolysinvariant L144S/D150E | Bacillus thermoproteolyticus | N-carbobenzoxy-L-Asp-L-Phe methyl ester | 17 | 0.17 | 99 | pH 7.5, 25 °C | Open record: thermolysin variant L144S/D150E, N-carbobenzoxy-L-Asp-L-Phe methyl ester |
| thermolysinvariant L144S/D150E/S53D | Bacillus thermoproteolyticus | N-[3-(2-furyl)acryloyl]-Gly-L-Leu amide0 M NaCl | — | — | 249 | pH 7.5, 25 °C | Open record: thermolysin variant L144S/D150E/S53D, N-[3-(2-furyl)acryloyl]-Gly-L-Leu amide |
| thermolysinvariant L144S/D150E/S53D | Bacillus thermoproteolyticus | N-[3-(2-furyl)acryloyl]-Gly-L-Leu amide4 M NaCl | — | — | 1.17 × 10³ | pH 7.5, 25 °C | Open record: thermolysin variant L144S/D150E/S53D, N-[3-(2-furyl)acryloyl]-Gly-L-Leu amide |
| thermolysinvariant L144S/D150E/S53D | Bacillus thermoproteolyticus | N-carbobenzoxy-L-Asp-L-Phe methyl ester | 15.6 | 0.16 | 96.2 | pH 7.5, 25 °C | Open record: thermolysin variant L144S/D150E/S53D, N-carbobenzoxy-L-Asp-L-Phe methyl ester |
| thermolysinvariant L155A | Bacillus thermoproteolyticus | N-[3-(2-furyl)acryloyl]-Gly-L-Leu amide0 M NaCl | — | — | 26 | pH 7.5, 25 °C | Open record: thermolysin variant L155A, N-[3-(2-furyl)acryloyl]-Gly-L-Leu amide |
| thermolysinvariant L155A | Bacillus thermoproteolyticus | N-[3-(2-furyl)acryloyl]-Gly-L-Leu amide4 M NaCl | — | — | 210 | pH 7.5, 25 °C | Open record: thermolysin variant L155A, N-[3-(2-furyl)acryloyl]-Gly-L-Leu amide |
| thermolysinvariant L155A | Bacillus thermoproteolyticus | N-carbobenzoxy-L-Asp-L-Phe methyl ester | 8.7 | 1.29 | 6.7 | pH 7.5, 25 °C | Open record: thermolysin variant L155A, N-carbobenzoxy-L-Asp-L-Phe methyl ester |
| thermolysinvariant L155A/G8C/N60C/S65P | Bacillus thermoproteolyticus | N-[3-(2-furyl)acryloyl]-Gly-L-Leu amide0 M NaCl | — | — | 34 | pH 7.5, 25 °C | Open record: thermolysin variant L155A/G8C/N60C/S65P, N-[3-(2-furyl)acryloyl]-Gly-L-Leu amide |
| thermolysinvariant L155A/G8C/N60C/S65P | Bacillus thermoproteolyticus | N-[3-(2-furyl)acryloyl]-Gly-L-Leu amide4 M NaCl | — | — | 430 | pH 7.5, 25 °C | Open record: thermolysin variant L155A/G8C/N60C/S65P, N-[3-(2-furyl)acryloyl]-Gly-L-Leu amide |
| thermolysinvariant L155A/G8C/N60C/S65P | Bacillus thermoproteolyticus | N-carbobenzoxy-L-Asp-L-Phe methyl ester | 6.3 | 0.83 | 7.5 | pH 7.5, 25 °C | Open record: thermolysin variant L155A/G8C/N60C/S65P, N-carbobenzoxy-L-Asp-L-Phe methyl ester |
| thermolysinvariant S53D | Bacillus thermoproteolyticus | N-[3-(2-furyl)acryloyl]-Gly-L-Leu amide0 M NaCl | — | — | 32 | pH 7.5, 25 °C | Open record: thermolysin variant S53D, N-[3-(2-furyl)acryloyl]-Gly-L-Leu amide |
| thermolysinvariant S53D | Bacillus thermoproteolyticus | N-[3-(2-furyl)acryloyl]-Gly-L-Leu amide4 M NaCl | — | — | 390 | pH 7.5, 25 °C | Open record: thermolysin variant S53D, N-[3-(2-furyl)acryloyl]-Gly-L-Leu amide |
| thermolysinvariant S53D | Bacillus thermoproteolyticus | N-carbobenzoxy-L-Asp-L-Phe methyl ester | 6.9 | 0.77 | 9 | pH 7.5, 25 °C | Open record: thermolysin variant S53D, N-carbobenzoxy-L-Asp-L-Phe methyl ester |
| thermolysinvariant S53D/G8C/N60C/S65P | Bacillus thermoproteolyticus | N-[3-(2-furyl)acryloyl]-Gly-L-Leu amide0 M NaCl | — | — | 31 | pH 7.5, 25 °C | Open record: thermolysin variant S53D/G8C/N60C/S65P, N-[3-(2-furyl)acryloyl]-Gly-L-Leu amide |
| thermolysinvariant S53D/G8C/N60C/S65P | Bacillus thermoproteolyticus | N-[3-(2-furyl)acryloyl]-Gly-L-Leu amide4 M NaCl | — | — | 400 | pH 7.5, 25 °C | Open record: thermolysin variant S53D/G8C/N60C/S65P, N-[3-(2-furyl)acryloyl]-Gly-L-Leu amide |
| thermolysinvariant S53D/G8C/N60C/S65P | Bacillus thermoproteolyticus | N-carbobenzoxy-L-Asp-L-Phe methyl ester | 7.4 | 0.94 | 7.9 | pH 7.5, 25 °C | Open record: thermolysin variant S53D/G8C/N60C/S65P, N-carbobenzoxy-L-Asp-L-Phe methyl ester |
| thermolysinvariant S53D/L155A | Bacillus thermoproteolyticus | N-[3-(2-furyl)acryloyl]-Gly-L-Leu amide0 M NaCl | — | — | 33 | pH 7.5, 25 °C | Open record: thermolysin variant S53D/L155A, N-[3-(2-furyl)acryloyl]-Gly-L-Leu amide |
| thermolysinvariant S53D/L155A | Bacillus thermoproteolyticus | N-[3-(2-furyl)acryloyl]-Gly-L-Leu amide4 M NaCl | — | — | 320 | pH 7.5, 25 °C | Open record: thermolysin variant S53D/L155A, N-[3-(2-furyl)acryloyl]-Gly-L-Leu amide |
| thermolysinvariant S53D/L155A | Bacillus thermoproteolyticus | N-carbobenzoxy-L-Asp-L-Phe methyl ester | 6.4 | 0.69 | 9.2 | pH 7.5, 25 °C | Open record: thermolysin variant S53D/L155A, N-carbobenzoxy-L-Asp-L-Phe methyl ester |
| thermolysinvariant S53D/L155A/G8C/N60C/S65P | Bacillus thermoproteolyticus | N-[3-(2-furyl)acryloyl]-Gly-L-Leu amide0 M NaCl | — | — | 23 | pH 7.5, 25 °C | Open record: thermolysin variant S53D/L155A/G8C/N60C/S65P, N-[3-(2-furyl)acryloyl]-Gly-L-Leu amide |
| thermolysinvariant S53D/L155A/G8C/N60C/S65P | Bacillus thermoproteolyticus | N-[3-(2-furyl)acryloyl]-Gly-L-Leu amide4 M NaCl | — | — | 330 | pH 7.5, 25 °C | Open record: thermolysin variant S53D/L155A/G8C/N60C/S65P, N-[3-(2-furyl)acryloyl]-Gly-L-Leu amide |
| thermolysinvariant S53D/L155A/G8C/N60C/S65P | Bacillus thermoproteolyticus | N-carbobenzoxy-L-Asp-L-Phe methyl ester | 3.3 | 0.55 | 6 | pH 7.5, 25 °C | Open record: thermolysin variant S53D/L155A/G8C/N60C/S65P, N-carbobenzoxy-L-Asp-L-Phe methyl ester |