EC 3.4.24.27: thermolysin

CatLog read 143 records for EC 3.4.24.27 from 6 papers, for 1 organism. Names in these papers: thermolysin.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units.

CatLog also holds 90 records for EC 3.4.24.27 that are not yet re-read: from its earlier version, built from the enzyme databases and partly checked against the papers. All records for EC 3.4.24.27 in Browse.

Papers

Papers with records for EC 3.4.24.27
Paper	Organisms	Records
Effects of the mutational combinations on the activity and stability of thermolysin	Bacillus thermoproteolyticus	42
Thermolysin: kinetic study with oligopeptides	Bacillus thermoproteolyticus	40
Effects of site-directed mutagenesis of Asn116 in the β-hairpin of the N-terminal domain of thermolysin on its activity and stability	Bacillus thermoproteolyticus	25
doi:10.1016/j.enzmictec.2009.12.001	Bacillus thermoproteolyticus	21
Additional data about thermolysin specificity in buffer- and glycerol-containing media	Bacillus thermoproteolyticus	12
Differences in transition state stabilization between thermolysin (EC 3.4.24.27) and neprilysin (EC 3.4.24.11)	Bacillus thermoproteolyticus	3

Records

Records for EC 3.4.24.27 read from the paper
Enzyme	Organism	Substrate	k_cat (s⁻¹)	K_m (mM)	k_cat/K_m (mM⁻¹ s⁻¹)	Conditions	Paper	Open record
thermolysin	Bacillus thermoproteolyticus	H-Gly-Phe-Ala-OHno glycerol	0.06	0.3	0.2	pH 8, 37 °C	PMID 9003446	Open record: thermolysin, H-Gly-Phe-Ala-OH
thermolysin	Bacillus thermoproteolyticus	H-Gly-Phe-Ala-OH5 M glycerol	0.077	0.6	0.128	pH 8, 37 °C	PMID 9003446	Open record: thermolysin, H-Gly-Phe-Ala-OH
thermolysin	Bacillus thermoproteolyticus	H-Gly-Phe-Leu-OHno glycerol	0.045	0.4	0.113	pH 8, 37 °C	PMID 9003446	Open record: thermolysin, H-Gly-Phe-Leu-OH
thermolysin	Bacillus thermoproteolyticus	H-Gly-Phe-Leu-OH5 M glycerol	0.027	1.8	0.015 calc.	pH 8, 37 °C	PMID 9003446	Open record: thermolysin, H-Gly-Phe-Leu-OH
thermolysin	Bacillus thermoproteolyticus	H-Gly-Phe-Leu-OHno glycerol	0.013	0.7	0.018	pH 8, 37 °C	PMID 9003446	Open record: thermolysin, H-Gly-Phe-Leu-OH
thermolysin	Bacillus thermoproteolyticus	H-Gly-Phe-Leu-OH5 M glycerol	0.0067	2.5	0.0033	pH 8, 37 °C	PMID 9003446	Open record: thermolysin, H-Gly-Phe-Leu-OH
thermolysin	Bacillus thermoproteolyticus	H-Gly-Phe-Ser-Ala-OHno glycerol	0.3	1.2	0.25	pH 8, 37 °C	PMID 9003446	Open record: thermolysin, H-Gly-Phe-Ser-Ala-OH
thermolysin	Bacillus thermoproteolyticus	H-Gly-Phe-Ser-Ala-OH5 M glycerol	0.592	12	0.05	pH 8, 37 °C	PMID 9003446	Open record: thermolysin, H-Gly-Phe-Ser-Ala-OH
thermolysin	Bacillus thermoproteolyticus	H-Gly-Phe-Ser-Ala-OHno glycerol	0.07	0.7	0.1	pH 8, 37 °C	PMID 9003446	Open record: thermolysin, H-Gly-Phe-Ser-Ala-OH
thermolysin	Bacillus thermoproteolyticus	H-Gly-Phe-Ser-Ala-OH5 M glycerol	0.173	6.8	0.025	pH 8, 37 °C	PMID 9003446	Open record: thermolysin, H-Gly-Phe-Ser-Ala-OH
thermolysin	Bacillus thermoproteolyticus	H-Gly-Phe-Ser-OHno glycerol	0.035	1.3	0.027	pH 8, 37 °C	PMID 9003446	Open record: thermolysin, H-Gly-Phe-Ser-OH
thermolysin	Bacillus thermoproteolyticus	H-Gly-Phe-Ser-OH5 M glycerol	0.05	3.5	0.014 calc.	pH 8, 37 °C	PMID 9003446	Open record: thermolysin, H-Gly-Phe-Ser-OH
thermolysin	Bacillus thermoproteolyticus	L-phenylalanine methyl ester	1.3	8.7	0.15 calc.	pH 7.5, 25 °C	doi:10.1016/j.enzmictec.2009.12.001	Open record: thermolysin, L-phenylalanine methyl ester
thermolysin	Bacillus thermoproteolyticus	L-phenylalanylglycyl-glycyl-L-leucyl-L-alanine	150.6	4.8	50.2	pH 7, 40 °C	PMID 4993757	Open record: thermolysin, L-phenylalanylglycyl-glycyl-L-leucyl-L-alanine
thermolysin	Bacillus thermoproteolyticus	L-phenylalanylglycyl-L-leucyl-L-alanine	53	4.6	11.5	pH 7, 40 °C	PMID 4993757	Open record: thermolysin, L-phenylalanylglycyl-L-leucyl-L-alanine
thermolysin	Bacillus thermoproteolyticus	N-[3-(2-furyl)acryloyl]-Gly-L-Leu amide0 M NaCl	—	—	29	pH 7.5, 25 °C	PMID 20214932	Open record: thermolysin, N-[3-(2-furyl)acryloyl]-Gly-L-Leu amide
thermolysin	Bacillus thermoproteolyticus	N-[3-(2-furyl)acryloyl]-Gly-L-Leu amide4 M NaCl	—	—	380	pH 7.5, 25 °C	PMID 20214932	Open record: thermolysin, N-[3-(2-furyl)acryloyl]-Gly-L-Leu amide
thermolysin	Bacillus thermoproteolyticus	N-[3-(2-furyl)acryloyl]-glycyl-L-leucine amidepH dependence in 40 mM acetate-NaOH (pH 4.0-5.5)	—	—	37	25 °C	PMID 22648563	Open record: thermolysin, N-[3-(2-furyl)acryloyl]-glycyl-L-leucine amide
thermolysin	Bacillus thermoproteolyticus	N-[3-(2-furyl)acryloyl]-glycyl-L-leucine amidewithout NaCl	—	—	30	pH 7.5, 25 °C	PMID 22648563	Open record: thermolysin, N-[3-(2-furyl)acryloyl]-glycyl-L-leucine amide
thermolysin	Bacillus thermoproteolyticus	N-[3-(2-furyl)acryloyl]-glycyl-L-leucine amidewith 4 M NaCl	—	—	440	pH 7.5, 25 °C	PMID 22648563	Open record: thermolysin, N-[3-(2-furyl)acryloyl]-glycyl-L-leucine amide
thermolysin	Bacillus thermoproteolyticus	N-[3-(2-furyl)acryloyl]-glycyl-L-leucine amide	—	—	29	25 °C	PMID 22648563	Open record: thermolysin, N-[3-(2-furyl)acryloyl]-glycyl-L-leucine amide
thermolysin	Bacillus thermoproteolyticus	N-benzyloxycarbonyl-D-alanylglycyl-glycyl-L-leucyl-L-alanine	127.5	7.1	18	pH 7, 40 °C	PMID 4993757	Open record: thermolysin, N-benzyloxycarbonyl-D-alanylglycyl-glycyl-L-leucyl-L-alanine
thermolysin	Bacillus thermoproteolyticus	N-benzyloxycarbonyl-D-alanylglycyl-L-leucyl-L-alanine	625	16	39	pH 7, 40 °C	PMID 4993757	Open record: thermolysin, N-benzyloxycarbonyl-D-alanylglycyl-L-leucyl-L-alanine
thermolysin	Bacillus thermoproteolyticus	N-benzyloxycarbonyl-L-alanyl-L-leucyl-L-alanine	996.9	4.9	203.4	pH 7, 40 °C	PMID 4993757	Open record: thermolysin, N-benzyloxycarbonyl-L-alanyl-L-leucyl-L-alanine
thermolysin	Bacillus thermoproteolyticus	N-benzyloxycarbonyl-L-alanylglycyl-glycyl-L-leucyl-L-alanine	50	7.5	6.6	pH 7, 40 °C	PMID 4993757	Open record: thermolysin, N-benzyloxycarbonyl-L-alanylglycyl-glycyl-L-leucyl-L-alanine
thermolysin	Bacillus thermoproteolyticus	N-benzyloxycarbonyl-L-alanylglycyl-L-leucyl-L-alanine	5208	8.6	605.6	pH 7, 40 °C	PMID 4993757	Open record: thermolysin, N-benzyloxycarbonyl-L-alanylglycyl-L-leucyl-L-alanine
thermolysin	Bacillus thermoproteolyticus	N-benzyloxycarbonyl-L-phenylalanyl-L-alanyl-L-alanine	21.9	2.6	8.4	pH 7, 40 °C	PMID 4993757	Open record: thermolysin, N-benzyloxycarbonyl-L-phenylalanyl-L-alanyl-L-alanine
thermolysin	Bacillus thermoproteolyticus	N-benzyloxycarbonyl-L-phenylalanyl-L-isoleucyl-L-alanine	148	0.82	205.5	pH 7, 40 °C	PMID 4993757	Open record: thermolysin, N-benzyloxycarbonyl-L-phenylalanyl-L-isoleucyl-L-alanine
thermolysin	Bacillus thermoproteolyticus	N-benzyloxycarbonyl-L-phenylalanyl-L-leucyl-L-alanineinitial substrate concentrations up to approx. 25 mM	398.3	0.58	678	pH 7, 40 °C	PMID 4993757	Open record: thermolysin, N-benzyloxycarbonyl-L-phenylalanyl-L-leucyl-L-alanine
thermolysin	Bacillus thermoproteolyticus	N-benzyloxycarbonyl-L-phenylalanyl-L-leucyl-L-alanine1.7% ethanol	416	0.72	577.8	pH 7, 40 °C	PMID 4993757	Open record: thermolysin, N-benzyloxycarbonyl-L-phenylalanyl-L-leucyl-L-alanine
thermolysin	Bacillus thermoproteolyticus	N-benzyloxycarbonyl-L-phenylalanyl-L-phenylalanyl-L-alanine	162	0.45	360	pH 7, 40 °C	PMID 4993757	Open record: thermolysin, N-benzyloxycarbonyl-L-phenylalanyl-L-phenylalanyl-L-alanine
thermolysin	Bacillus thermoproteolyticus	N-benzyloxycarbonyl-L-phenylalanyl-L-seryl-L-alanine	3	1.6	1.9	pH 7, 40 °C	PMID 4993757	Open record: thermolysin, N-benzyloxycarbonyl-L-phenylalanyl-L-seryl-L-alanine
thermolysin	Bacillus thermoproteolyticus	N-benzyloxycarbonyl-L-phenylalanyl-L-tyrosyl-L-alanine	7.1	0.72	9.9	pH 7, 40 °C	PMID 4993757	Open record: thermolysin, N-benzyloxycarbonyl-L-phenylalanyl-L-tyrosyl-L-alanine
thermolysin	Bacillus thermoproteolyticus	N-benzyloxycarbonyl-L-phenylalanyl-L-valyl-L-alanine	169	0.78	217	pH 7, 40 °C	PMID 4993757	Open record: thermolysin, N-benzyloxycarbonyl-L-phenylalanyl-L-valyl-L-alanine
thermolysin	Bacillus thermoproteolyticus	N-benzyloxycarbonyl-L-phenylalanylglycyl-glycyl-L-leucyl-L-alanine	21.9	3	7.3	pH 7, 40 °C	PMID 4993757	Open record: thermolysin, N-benzyloxycarbonyl-L-phenylalanylglycyl-glycyl-L-leucyl-L-alanine
thermolysin	Bacillus thermoproteolyticus	N-benzyloxycarbonyl-L-phenylalanylglycyl-L-alanine	7.8	7.4	1.05	pH 7, 40 °C	PMID 4993757	Open record: thermolysin, N-benzyloxycarbonyl-L-phenylalanylglycyl-L-alanine
thermolysin	Bacillus thermoproteolyticus	N-benzyloxycarbonyl-L-phenylalanylglycyl-L-leucyl-L-alanine	446.4	0.91	490.5	pH 7, 40 °C	PMID 4993757	Open record: thermolysin, N-benzyloxycarbonyl-L-phenylalanylglycyl-L-leucyl-L-alanine
thermolysin	Bacillus thermoproteolyticus	N-benzyloxycarbonyl-L-tyrosyl-glycinamide	0.125	20.8	0.006	pH 7, 40 °C	PMID 4993757	Open record: thermolysin, N-benzyloxycarbonyl-L-tyrosyl-glycinamide
thermolysin	Bacillus thermoproteolyticus	N-benzyloxycarbonyl-L-tyrosyl-L-serinamide	0.36	2.3	0.16	pH 7, 40 °C	PMID 4993757	Open record: thermolysin, N-benzyloxycarbonyl-L-tyrosyl-L-serinamide
thermolysin	Bacillus thermoproteolyticus	N-benzyloxycarbonylglycyl-L-alaninamide	44.5	600	0.074	pH 7, 40 °C	PMID 4993757	Open record: thermolysin, N-benzyloxycarbonylglycyl-L-alaninamide
thermolysin	Bacillus thermoproteolyticus	N-benzyloxycarbonylglycyl-L-isoleucinamide	59.3	33.3	1.8	pH 7, 40 °C	PMID 4993757	Open record: thermolysin, N-benzyloxycarbonylglycyl-L-isoleucinamide
thermolysin	Bacillus thermoproteolyticus	N-benzyloxycarbonylglycyl-L-leucinamideinitial substrate concentrations up to approx. 25 mM	105	20.6	5.1	pH 7, 40 °C	PMID 4993757	Open record: thermolysin, N-benzyloxycarbonylglycyl-L-leucinamide
thermolysin	Bacillus thermoproteolyticus	N-benzyloxycarbonylglycyl-L-leucinamide1.7% ethanol	89	33.3	2.7	pH 7, 40 °C	PMID 4993757	Open record: thermolysin, N-benzyloxycarbonylglycyl-L-leucinamide
thermolysin	Bacillus thermoproteolyticus	N-benzyloxycarbonylglycyl-L-leucyl-D-alanine	5.2	16.6	0.3	pH 7, 40 °C	PMID 4993757	Open record: thermolysin, N-benzyloxycarbonylglycyl-L-leucyl-D-alanine
thermolysin	Bacillus thermoproteolyticus	N-benzyloxycarbonylglycyl-L-leucyl-L-alanine	780.6	10.6	78.1	pH 7, 40 °C	PMID 4993757	Open record: thermolysin, N-benzyloxycarbonylglycyl-L-leucyl-L-alanine
thermolysin	Bacillus thermoproteolyticus	N-benzyloxycarbonylglycyl-L-leucyl-L-leucine	374	2.6	143.9	pH 7, 40 °C	PMID 4993757	Open record: thermolysin, N-benzyloxycarbonylglycyl-L-leucyl-L-leucine
thermolysin	Bacillus thermoproteolyticus	N-benzyloxycarbonylglycyl-L-leucyl-L-phenylalanine	120	2.4	50	pH 7, 40 °C	PMID 4993757	Open record: thermolysin, N-benzyloxycarbonylglycyl-L-leucyl-L-phenylalanine
thermolysin	Bacillus thermoproteolyticus	N-benzyloxycarbonylglycyl-L-leucylglycine	65.4	10.8	6.1	pH 7, 40 °C	PMID 4993757	Open record: thermolysin, N-benzyloxycarbonylglycyl-L-leucylglycine
thermolysin	Bacillus thermoproteolyticus	N-benzyloxycarbonylglycyl-L-leucylglycyl-D-alanine	25	13.4	1.9	pH 7, 40 °C	PMID 4993757	Open record: thermolysin, N-benzyloxycarbonylglycyl-L-leucylglycyl-D-alanine
thermolysin	Bacillus thermoproteolyticus	N-benzyloxycarbonylglycyl-L-leucylglycyl-glycine	62.4	11.8	5.3	pH 7, 40 °C	PMID 4993757	Open record: thermolysin, N-benzyloxycarbonylglycyl-L-leucylglycyl-glycine
thermolysin	Bacillus thermoproteolyticus	N-benzyloxycarbonylglycyl-L-leucylglycyl-L-alanine	418.9	13.6	30.8	pH 7, 40 °C	PMID 4993757	Open record: thermolysin, N-benzyloxycarbonylglycyl-L-leucylglycyl-L-alanine
thermolysin	Bacillus thermoproteolyticus	N-benzyloxycarbonylglycyl-L-leucylglycyl-L-phenylalanine	131.6	11	12	pH 7, 40 °C	PMID 4993757	Open record: thermolysin, N-benzyloxycarbonylglycyl-L-leucylglycyl-L-phenylalanine
thermolysin	Bacillus thermoproteolyticus	N-benzyloxycarbonylglycyl-L-phenylalaninamide	38.5	14.7	2.6	pH 7, 40 °C	PMID 4993757	Open record: thermolysin, N-benzyloxycarbonylglycyl-L-phenylalaninamide
thermolysin	Bacillus thermoproteolyticus	N-benzyloxycarbonylglycyl-L-prolyl-L-leucylglycine	11.2	12.8	0.875 calc.	pH 7, 40 °C	PMID 4993757	Open record: thermolysin, N-benzyloxycarbonylglycyl-L-prolyl-L-leucylglycine
thermolysin	Bacillus thermoproteolyticus	N-benzyloxycarbonylglycyl-L-prolyl-L-leucylglycyl-L-proline	41.6	13.3	3.13 calc.	pH 7, 40 °C	PMID 4993757	Open record: thermolysin, N-benzyloxycarbonylglycyl-L-prolyl-L-leucylglycyl-L-proline
thermolysin	Bacillus thermoproteolyticus	N-benzyloxycarbonylglycyl-L-serinamide	1.6	600	0.003	pH 7, 40 °C	PMID 4993757	Open record: thermolysin, N-benzyloxycarbonylglycyl-L-serinamide
thermolysin	Bacillus thermoproteolyticus	N-benzyloxycarbonylglycyl-L-tyrosinamide	1.6	14.7	0.11	pH 7, 40 °C	PMID 4993757	Open record: thermolysin, N-benzyloxycarbonylglycyl-L-tyrosinamide
thermolysin	Bacillus thermoproteolyticus	N-benzyloxycarbonylglycyl-L-valinamide	22.6	71.5	0.32	pH 7, 40 °C	PMID 4993757	Open record: thermolysin, N-benzyloxycarbonylglycyl-L-valinamide
thermolysin	Bacillus thermoproteolyticus	N-benzyloxycarbonylglycylglycyl-L-leucyl-L-alanine	362	12.2	29.7	pH 7, 40 °C	PMID 4993757	Open record: thermolysin, N-benzyloxycarbonylglycylglycyl-L-leucyl-L-alanine
thermolysin	Bacillus thermoproteolyticus	N-carbobenzoxy-L-Asp-L-Phe methyl ester	5.9	0.63	9.4	pH 7.5, 25 °C	PMID 20214932	Open record: thermolysin, N-carbobenzoxy-L-Asp-L-Phe methyl ester
thermolysin	Bacillus thermoproteolyticus	N-carbobenzoxy-L-aspartic acid	—	62	—	pH 7.5, 25 °C	doi:10.1016/j.enzmictec.2009.12.001	Open record: thermolysin, N-carbobenzoxy-L-aspartic acid
thermolysin	Bacillus thermoproteolyticus	N-carbobenzoxy-L-aspartyl-L-phenylalanine methyl ester	7.7	0.39	20	pH 7.5, 25 °C	PMID 22648563	Open record: thermolysin, N-carbobenzoxy-L-aspartyl-L-phenylalanine methyl ester
thermolysin	Bacillus thermoproteolyticus	N-carbobenzoxy-L-aspartyl-L-phenylalanine methyl ester	9.5	1	9.3	pH 7.5, 25 °C	doi:10.1016/j.enzmictec.2009.12.001	Open record: thermolysin, N-carbobenzoxy-L-aspartyl-L-phenylalanine methyl ester
thermolysin	Bacillus thermoproteolyticus	tyrosyl-glycyl-glycyl-phenylalanyl-leucine	155.5	0.21	740		PMID 9827548	Open record: thermolysin, tyrosyl-glycyl-glycyl-phenylalanyl-leucine
thermolysinvariant D150A	Bacillus thermoproteolyticus	L-phenylalanine methyl ester	2.1	7.3	0.29 calc.	pH 7.5, 25 °C	doi:10.1016/j.enzmictec.2009.12.001	Open record: thermolysin variant D150A, L-phenylalanine methyl ester
thermolysinvariant D150A	Bacillus thermoproteolyticus	N-carbobenzoxy-L-aspartic acid	—	57	—	pH 7.5, 25 °C	doi:10.1016/j.enzmictec.2009.12.001	Open record: thermolysin variant D150A, N-carbobenzoxy-L-aspartic acid
thermolysinvariant D150A	Bacillus thermoproteolyticus	N-carbobenzoxy-L-aspartyl-L-phenylalanine methyl ester	24.4	2.1	11.7	pH 7.5, 25 °C	doi:10.1016/j.enzmictec.2009.12.001	Open record: thermolysin variant D150A, N-carbobenzoxy-L-aspartyl-L-phenylalanine methyl ester
thermolysinvariant D150E	Bacillus thermoproteolyticus	L-phenylalanine methyl ester	2.5	5.8	0.43 calc.	pH 7.5, 25 °C	doi:10.1016/j.enzmictec.2009.12.001	Open record: thermolysin variant D150E, L-phenylalanine methyl ester
thermolysinvariant D150E	Bacillus thermoproteolyticus	N-[3-(2-furyl)acryloyl]-Gly-L-Leu amide0 M NaCl	—	—	79	pH 7.5, 25 °C	PMID 20214932	Open record: thermolysin variant D150E, N-[3-(2-furyl)acryloyl]-Gly-L-Leu amide
thermolysinvariant D150E	Bacillus thermoproteolyticus	N-[3-(2-furyl)acryloyl]-Gly-L-Leu amide4 M NaCl	—	—	400	pH 7.5, 25 °C	PMID 20214932	Open record: thermolysin variant D150E, N-[3-(2-furyl)acryloyl]-Gly-L-Leu amide
thermolysinvariant D150E	Bacillus thermoproteolyticus	N-carbobenzoxy-L-Asp-L-Phe methyl ester	16.5	0.5	33.1	pH 7.5, 25 °C	PMID 20214932	Open record: thermolysin variant D150E, N-carbobenzoxy-L-Asp-L-Phe methyl ester
thermolysinvariant D150E	Bacillus thermoproteolyticus	N-carbobenzoxy-L-aspartic acid	—	60	—	pH 7.5, 25 °C	doi:10.1016/j.enzmictec.2009.12.001	Open record: thermolysin variant D150E, N-carbobenzoxy-L-aspartic acid
thermolysinvariant D150E	Bacillus thermoproteolyticus	N-carbobenzoxy-L-aspartyl-L-phenylalanine methyl ester	20.5	0.7	27.6	pH 7.5, 25 °C	doi:10.1016/j.enzmictec.2009.12.001	Open record: thermolysin variant D150E, N-carbobenzoxy-L-aspartyl-L-phenylalanine methyl ester
thermolysinvariant D150E/I168A	Bacillus thermoproteolyticus	N-[3-(2-furyl)acryloyl]-Gly-L-Leu amide0 M NaCl	—	—	141	pH 7.5, 25 °C	PMID 20214932	Open record: thermolysin variant D150E/I168A, N-[3-(2-furyl)acryloyl]-Gly-L-Leu amide
thermolysinvariant D150E/I168A	Bacillus thermoproteolyticus	N-[3-(2-furyl)acryloyl]-Gly-L-Leu amide4 M NaCl	—	—	600	pH 7.5, 25 °C	PMID 20214932	Open record: thermolysin variant D150E/I168A, N-[3-(2-furyl)acryloyl]-Gly-L-Leu amide
thermolysinvariant D150E/I168A	Bacillus thermoproteolyticus	N-carbobenzoxy-L-Asp-L-Phe methyl ester	15.5	0.18	83.8	pH 7.5, 25 °C	PMID 20214932	Open record: thermolysin variant D150E/I168A, N-carbobenzoxy-L-Asp-L-Phe methyl ester
thermolysinvariant D150W	Bacillus thermoproteolyticus	L-phenylalanine methyl ester	3.2	7	0.46 calc.	pH 7.5, 25 °C	doi:10.1016/j.enzmictec.2009.12.001	Open record: thermolysin variant D150W, L-phenylalanine methyl ester
thermolysinvariant D150W	Bacillus thermoproteolyticus	N-carbobenzoxy-L-aspartic acid	—	54	—	pH 7.5, 25 °C	doi:10.1016/j.enzmictec.2009.12.001	Open record: thermolysin variant D150W, N-carbobenzoxy-L-aspartic acid
thermolysinvariant D150W	Bacillus thermoproteolyticus	N-carbobenzoxy-L-aspartyl-L-phenylalanine methyl ester	49.4	2.1	23	pH 7.5, 25 °C	doi:10.1016/j.enzmictec.2009.12.001	Open record: thermolysin variant D150W, N-carbobenzoxy-L-aspartyl-L-phenylalanine methyl ester
thermolysinvariant D226A	Bacillus thermoproteolyticus	tyrosyl-glycyl-glycyl-phenylalanyl-leucine	37.53	1.985	18.8		PMID 9827548	Open record: thermolysin variant D226A, tyrosyl-glycyl-glycyl-phenylalanyl-leucine
thermolysinvariant F114A	Bacillus thermoproteolyticus	L-phenylalanine methyl ester	0.3	11.3	0.027 calc.	pH 7.5, 25 °C	doi:10.1016/j.enzmictec.2009.12.001	Open record: thermolysin variant F114A, L-phenylalanine methyl ester
thermolysinvariant F114A	Bacillus thermoproteolyticus	N-carbobenzoxy-L-aspartic acid	—	49	—	pH 7.5, 25 °C	doi:10.1016/j.enzmictec.2009.12.001	Open record: thermolysin variant F114A, N-carbobenzoxy-L-aspartic acid
thermolysinvariant F114A	Bacillus thermoproteolyticus	N-carbobenzoxy-L-aspartyl-L-phenylalanine methyl ester	1.5	1	1.5	pH 7.5, 25 °C	doi:10.1016/j.enzmictec.2009.12.001	Open record: thermolysin variant F114A, N-carbobenzoxy-L-aspartyl-L-phenylalanine methyl ester
thermolysinvariant G8C/N60C/S65P	Bacillus thermoproteolyticus	N-[3-(2-furyl)acryloyl]-Gly-L-Leu amide0 M NaCl	—	—	29	pH 7.5, 25 °C	PMID 20214932	Open record: thermolysin variant G8C/N60C/S65P, N-[3-(2-furyl)acryloyl]-Gly-L-Leu amide
thermolysinvariant G8C/N60C/S65P	Bacillus thermoproteolyticus	N-[3-(2-furyl)acryloyl]-Gly-L-Leu amide4 M NaCl	—	—	350	pH 7.5, 25 °C	PMID 20214932	Open record: thermolysin variant G8C/N60C/S65P, N-[3-(2-furyl)acryloyl]-Gly-L-Leu amide
thermolysinvariant G8C/N60C/S65P	Bacillus thermoproteolyticus	N-carbobenzoxy-L-Asp-L-Phe methyl ester	5.4	0.54	10	pH 7.5, 25 °C	PMID 20214932	Open record: thermolysin variant G8C/N60C/S65P, N-carbobenzoxy-L-Asp-L-Phe methyl ester
thermolysinvariant I168A	Bacillus thermoproteolyticus	L-phenylalanine methyl ester	4.9	12.9	0.38 calc.	pH 7.5, 25 °C	doi:10.1016/j.enzmictec.2009.12.001	Open record: thermolysin variant I168A, L-phenylalanine methyl ester
thermolysinvariant I168A	Bacillus thermoproteolyticus	N-[3-(2-furyl)acryloyl]-Gly-L-Leu amide0 M NaCl	—	—	62	pH 7.5, 25 °C	PMID 20214932	Open record: thermolysin variant I168A, N-[3-(2-furyl)acryloyl]-Gly-L-Leu amide
thermolysinvariant I168A	Bacillus thermoproteolyticus	N-[3-(2-furyl)acryloyl]-Gly-L-Leu amide4 M NaCl	—	—	300	pH 7.5, 25 °C	PMID 20214932	Open record: thermolysin variant I168A, N-[3-(2-furyl)acryloyl]-Gly-L-Leu amide
thermolysinvariant I168A	Bacillus thermoproteolyticus	N-carbobenzoxy-L-Asp-L-Phe methyl ester	7.7	0.46	16.8	pH 7.5, 25 °C	PMID 20214932	Open record: thermolysin variant I168A, N-carbobenzoxy-L-Asp-L-Phe methyl ester
thermolysinvariant I168A	Bacillus thermoproteolyticus	N-carbobenzoxy-L-aspartic acid	—	105	—	pH 7.5, 25 °C	doi:10.1016/j.enzmictec.2009.12.001	Open record: thermolysin variant I168A, N-carbobenzoxy-L-aspartic acid
thermolysinvariant I168A	Bacillus thermoproteolyticus	N-carbobenzoxy-L-aspartyl-L-phenylalanine methyl ester	12.3	0.8	16	pH 7.5, 25 °C	doi:10.1016/j.enzmictec.2009.12.001	Open record: thermolysin variant I168A, N-carbobenzoxy-L-aspartyl-L-phenylalanine methyl ester
thermolysinvariant L144S	Bacillus thermoproteolyticus	N-[3-(2-furyl)acryloyl]-Gly-L-Leu amide0 M NaCl	—	—	182	pH 7.5, 25 °C	PMID 20214932	Open record: thermolysin variant L144S, N-[3-(2-furyl)acryloyl]-Gly-L-Leu amide
thermolysinvariant L144S	Bacillus thermoproteolyticus	N-[3-(2-furyl)acryloyl]-Gly-L-Leu amide4 M NaCl	—	—	660	pH 7.5, 25 °C	PMID 20214932	Open record: thermolysin variant L144S, N-[3-(2-furyl)acryloyl]-Gly-L-Leu amide
thermolysinvariant L144S	Bacillus thermoproteolyticus	N-carbobenzoxy-L-Asp-L-Phe methyl ester	9.4	0.19	49.7	pH 7.5, 25 °C	PMID 20214932	Open record: thermolysin variant L144S, N-carbobenzoxy-L-Asp-L-Phe methyl ester
thermolysinvariant L144S/D150E	Bacillus thermoproteolyticus	N-[3-(2-furyl)acryloyl]-Gly-L-Leu amide0 M NaCl	—	—	204	pH 7.5, 25 °C	PMID 20214932	Open record: thermolysin variant L144S/D150E, N-[3-(2-furyl)acryloyl]-Gly-L-Leu amide
thermolysinvariant L144S/D150E	Bacillus thermoproteolyticus	N-[3-(2-furyl)acryloyl]-Gly-L-Leu amide4 M NaCl	—	—	590	pH 7.5, 25 °C	PMID 20214932	Open record: thermolysin variant L144S/D150E, N-[3-(2-furyl)acryloyl]-Gly-L-Leu amide
thermolysinvariant L144S/D150E	Bacillus thermoproteolyticus	N-carbobenzoxy-L-Asp-L-Phe methyl ester	17	0.17	99	pH 7.5, 25 °C	PMID 20214932	Open record: thermolysin variant L144S/D150E, N-carbobenzoxy-L-Asp-L-Phe methyl ester
thermolysinvariant L144S/D150E/S53D	Bacillus thermoproteolyticus	N-[3-(2-furyl)acryloyl]-Gly-L-Leu amide0 M NaCl	—	—	249	pH 7.5, 25 °C	PMID 20214932	Open record: thermolysin variant L144S/D150E/S53D, N-[3-(2-furyl)acryloyl]-Gly-L-Leu amide
thermolysinvariant L144S/D150E/S53D	Bacillus thermoproteolyticus	N-[3-(2-furyl)acryloyl]-Gly-L-Leu amide4 M NaCl	—	—	1.17 × 10³	pH 7.5, 25 °C	PMID 20214932	Open record: thermolysin variant L144S/D150E/S53D, N-[3-(2-furyl)acryloyl]-Gly-L-Leu amide
thermolysinvariant L144S/D150E/S53D	Bacillus thermoproteolyticus	N-carbobenzoxy-L-Asp-L-Phe methyl ester	15.6	0.16	96.2	pH 7.5, 25 °C	PMID 20214932	Open record: thermolysin variant L144S/D150E/S53D, N-carbobenzoxy-L-Asp-L-Phe methyl ester
thermolysinvariant L155A	Bacillus thermoproteolyticus	N-[3-(2-furyl)acryloyl]-Gly-L-Leu amide0 M NaCl	—	—	26	pH 7.5, 25 °C	PMID 20214932	Open record: thermolysin variant L155A, N-[3-(2-furyl)acryloyl]-Gly-L-Leu amide
thermolysinvariant L155A	Bacillus thermoproteolyticus	N-[3-(2-furyl)acryloyl]-Gly-L-Leu amide4 M NaCl	—	—	210	pH 7.5, 25 °C	PMID 20214932	Open record: thermolysin variant L155A, N-[3-(2-furyl)acryloyl]-Gly-L-Leu amide
thermolysinvariant L155A	Bacillus thermoproteolyticus	N-carbobenzoxy-L-Asp-L-Phe methyl ester	8.7	1.29	6.7	pH 7.5, 25 °C	PMID 20214932	Open record: thermolysin variant L155A, N-carbobenzoxy-L-Asp-L-Phe methyl ester
thermolysinvariant L155A/G8C/N60C/S65P	Bacillus thermoproteolyticus	N-[3-(2-furyl)acryloyl]-Gly-L-Leu amide0 M NaCl	—	—	34	pH 7.5, 25 °C	PMID 20214932	Open record: thermolysin variant L155A/G8C/N60C/S65P, N-[3-(2-furyl)acryloyl]-Gly-L-Leu amide
thermolysinvariant L155A/G8C/N60C/S65P	Bacillus thermoproteolyticus	N-[3-(2-furyl)acryloyl]-Gly-L-Leu amide4 M NaCl	—	—	430	pH 7.5, 25 °C	PMID 20214932	Open record: thermolysin variant L155A/G8C/N60C/S65P, N-[3-(2-furyl)acryloyl]-Gly-L-Leu amide
thermolysinvariant L155A/G8C/N60C/S65P	Bacillus thermoproteolyticus	N-carbobenzoxy-L-Asp-L-Phe methyl ester	6.3	0.83	7.5	pH 7.5, 25 °C	PMID 20214932	Open record: thermolysin variant L155A/G8C/N60C/S65P, N-carbobenzoxy-L-Asp-L-Phe methyl ester
thermolysinvariant N116A	Bacillus thermoproteolyticus	N-[3-(2-furyl)acryloyl]-glycyl-L-leucine amidepH dependence in 40 mM acetate-NaOH (pH 4.0-5.5)	—	—	31	25 °C	PMID 22648563	Open record: thermolysin variant N116A, N-[3-(2-furyl)acryloyl]-glycyl-L-leucine amide
thermolysinvariant N116A	Bacillus thermoproteolyticus	N-[3-(2-furyl)acryloyl]-glycyl-L-leucine amidewithout NaCl	—	—	24	pH 7.5, 25 °C	PMID 22648563	Open record: thermolysin variant N116A, N-[3-(2-furyl)acryloyl]-glycyl-L-leucine amide
thermolysinvariant N116A	Bacillus thermoproteolyticus	N-[3-(2-furyl)acryloyl]-glycyl-L-leucine amidewith 4 M NaCl	—	—	330	pH 7.5, 25 °C	PMID 22648563	Open record: thermolysin variant N116A, N-[3-(2-furyl)acryloyl]-glycyl-L-leucine amide
thermolysinvariant N116A	Bacillus thermoproteolyticus	N-[3-(2-furyl)acryloyl]-glycyl-L-leucine amide	—	—	23	25 °C	PMID 22648563	Open record: thermolysin variant N116A, N-[3-(2-furyl)acryloyl]-glycyl-L-leucine amide
thermolysinvariant N116A	Bacillus thermoproteolyticus	N-carbobenzoxy-L-aspartyl-L-phenylalanine methyl ester	6.7	0.41	16	pH 7.5, 25 °C	PMID 22648563	Open record: thermolysin variant N116A, N-carbobenzoxy-L-aspartyl-L-phenylalanine methyl ester
thermolysinvariant N116D	Bacillus thermoproteolyticus	N-[3-(2-furyl)acryloyl]-glycyl-L-leucine amidepH dependence in 40 mM acetate-NaOH (pH 4.0-5.5)	—	—	117	25 °C	PMID 22648563	Open record: thermolysin variant N116D, N-[3-(2-furyl)acryloyl]-glycyl-L-leucine amide
thermolysinvariant N116D	Bacillus thermoproteolyticus	N-[3-(2-furyl)acryloyl]-glycyl-L-leucine amidewithout NaCl	—	—	95	pH 7.5, 25 °C	PMID 22648563	Open record: thermolysin variant N116D, N-[3-(2-furyl)acryloyl]-glycyl-L-leucine amide
thermolysinvariant N116D	Bacillus thermoproteolyticus	N-[3-(2-furyl)acryloyl]-glycyl-L-leucine amidewith 4 M NaCl	—	—	1 × 10³	pH 7.5, 25 °C	PMID 22648563	Open record: thermolysin variant N116D, N-[3-(2-furyl)acryloyl]-glycyl-L-leucine amide
thermolysinvariant N116D	Bacillus thermoproteolyticus	N-[3-(2-furyl)acryloyl]-glycyl-L-leucine amide	—	—	95	25 °C	PMID 22648563	Open record: thermolysin variant N116D, N-[3-(2-furyl)acryloyl]-glycyl-L-leucine amide
thermolysinvariant N116D	Bacillus thermoproteolyticus	N-carbobenzoxy-L-aspartyl-L-phenylalanine methyl ester	12	0.43	27	pH 7.5, 25 °C	PMID 22648563	Open record: thermolysin variant N116D, N-carbobenzoxy-L-aspartyl-L-phenylalanine methyl ester
thermolysinvariant N116Q	Bacillus thermoproteolyticus	N-[3-(2-furyl)acryloyl]-glycyl-L-leucine amidepH dependence in 40 mM acetate-NaOH (pH 4.0-5.5)	—	—	37	25 °C	PMID 22648563	Open record: thermolysin variant N116Q, N-[3-(2-furyl)acryloyl]-glycyl-L-leucine amide
thermolysinvariant N116Q	Bacillus thermoproteolyticus	N-[3-(2-furyl)acryloyl]-glycyl-L-leucine amidewithout NaCl	—	—	31	pH 7.5, 25 °C	PMID 22648563	Open record: thermolysin variant N116Q, N-[3-(2-furyl)acryloyl]-glycyl-L-leucine amide
thermolysinvariant N116Q	Bacillus thermoproteolyticus	N-[3-(2-furyl)acryloyl]-glycyl-L-leucine amidewith 4 M NaCl	—	—	270	pH 7.5, 25 °C	PMID 22648563	Open record: thermolysin variant N116Q, N-[3-(2-furyl)acryloyl]-glycyl-L-leucine amide
thermolysinvariant N116Q	Bacillus thermoproteolyticus	N-[3-(2-furyl)acryloyl]-glycyl-L-leucine amide	—	—	29	25 °C	PMID 22648563	Open record: thermolysin variant N116Q, N-[3-(2-furyl)acryloyl]-glycyl-L-leucine amide
thermolysinvariant N116Q	Bacillus thermoproteolyticus	N-carbobenzoxy-L-aspartyl-L-phenylalanine methyl ester	7.1	0.38	19	pH 7.5, 25 °C	PMID 22648563	Open record: thermolysin variant N116Q, N-carbobenzoxy-L-aspartyl-L-phenylalanine methyl ester
thermolysinvariant N116T	Bacillus thermoproteolyticus	N-[3-(2-furyl)acryloyl]-glycyl-L-leucine amidepH dependence in 40 mM acetate-NaOH (pH 4.0-5.5)	—	—	26	25 °C	PMID 22648563	Open record: thermolysin variant N116T, N-[3-(2-furyl)acryloyl]-glycyl-L-leucine amide
thermolysinvariant N116T	Bacillus thermoproteolyticus	N-[3-(2-furyl)acryloyl]-glycyl-L-leucine amidewithout NaCl	—	—	24	pH 7.5, 25 °C	PMID 22648563	Open record: thermolysin variant N116T, N-[3-(2-furyl)acryloyl]-glycyl-L-leucine amide
thermolysinvariant N116T	Bacillus thermoproteolyticus	N-[3-(2-furyl)acryloyl]-glycyl-L-leucine amidewith 4 M NaCl	—	—	240	pH 7.5, 25 °C	PMID 22648563	Open record: thermolysin variant N116T, N-[3-(2-furyl)acryloyl]-glycyl-L-leucine amide
thermolysinvariant N116T	Bacillus thermoproteolyticus	N-[3-(2-furyl)acryloyl]-glycyl-L-leucine amide	—	—	23	25 °C	PMID 22648563	Open record: thermolysin variant N116T, N-[3-(2-furyl)acryloyl]-glycyl-L-leucine amide
thermolysinvariant N116T	Bacillus thermoproteolyticus	N-carbobenzoxy-L-aspartyl-L-phenylalanine methyl ester	5.4	0.49	11	pH 7.5, 25 °C	PMID 22648563	Open record: thermolysin variant N116T, N-carbobenzoxy-L-aspartyl-L-phenylalanine methyl ester
thermolysinvariant N227H	Bacillus thermoproteolyticus	L-phenylalanine methyl ester	2.3	8.8	0.26 calc.	pH 7.5, 25 °C	doi:10.1016/j.enzmictec.2009.12.001	Open record: thermolysin variant N227H, L-phenylalanine methyl ester
thermolysinvariant N227H	Bacillus thermoproteolyticus	N-carbobenzoxy-L-aspartic acid	—	70	—	pH 7.5, 25 °C	doi:10.1016/j.enzmictec.2009.12.001	Open record: thermolysin variant N227H, N-carbobenzoxy-L-aspartic acid
thermolysinvariant N227H	Bacillus thermoproteolyticus	N-carbobenzoxy-L-aspartyl-L-phenylalanine methyl ester	17.1	1.3	13.7	pH 7.5, 25 °C	doi:10.1016/j.enzmictec.2009.12.001	Open record: thermolysin variant N227H, N-carbobenzoxy-L-aspartyl-L-phenylalanine methyl ester
thermolysinvariant S53D	Bacillus thermoproteolyticus	N-[3-(2-furyl)acryloyl]-Gly-L-Leu amide0 M NaCl	—	—	32	pH 7.5, 25 °C	PMID 20214932	Open record: thermolysin variant S53D, N-[3-(2-furyl)acryloyl]-Gly-L-Leu amide
thermolysinvariant S53D	Bacillus thermoproteolyticus	N-[3-(2-furyl)acryloyl]-Gly-L-Leu amide4 M NaCl	—	—	390	pH 7.5, 25 °C	PMID 20214932	Open record: thermolysin variant S53D, N-[3-(2-furyl)acryloyl]-Gly-L-Leu amide
thermolysinvariant S53D	Bacillus thermoproteolyticus	N-carbobenzoxy-L-Asp-L-Phe methyl ester	6.9	0.77	9	pH 7.5, 25 °C	PMID 20214932	Open record: thermolysin variant S53D, N-carbobenzoxy-L-Asp-L-Phe methyl ester
thermolysinvariant S53D/G8C/N60C/S65P	Bacillus thermoproteolyticus	N-[3-(2-furyl)acryloyl]-Gly-L-Leu amide0 M NaCl	—	—	31	pH 7.5, 25 °C	PMID 20214932	Open record: thermolysin variant S53D/G8C/N60C/S65P, N-[3-(2-furyl)acryloyl]-Gly-L-Leu amide
thermolysinvariant S53D/G8C/N60C/S65P	Bacillus thermoproteolyticus	N-[3-(2-furyl)acryloyl]-Gly-L-Leu amide4 M NaCl	—	—	400	pH 7.5, 25 °C	PMID 20214932	Open record: thermolysin variant S53D/G8C/N60C/S65P, N-[3-(2-furyl)acryloyl]-Gly-L-Leu amide
thermolysinvariant S53D/G8C/N60C/S65P	Bacillus thermoproteolyticus	N-carbobenzoxy-L-Asp-L-Phe methyl ester	7.4	0.94	7.9	pH 7.5, 25 °C	PMID 20214932	Open record: thermolysin variant S53D/G8C/N60C/S65P, N-carbobenzoxy-L-Asp-L-Phe methyl ester
thermolysinvariant S53D/L155A	Bacillus thermoproteolyticus	N-[3-(2-furyl)acryloyl]-Gly-L-Leu amide0 M NaCl	—	—	33	pH 7.5, 25 °C	PMID 20214932	Open record: thermolysin variant S53D/L155A, N-[3-(2-furyl)acryloyl]-Gly-L-Leu amide
thermolysinvariant S53D/L155A	Bacillus thermoproteolyticus	N-[3-(2-furyl)acryloyl]-Gly-L-Leu amide4 M NaCl	—	—	320	pH 7.5, 25 °C	PMID 20214932	Open record: thermolysin variant S53D/L155A, N-[3-(2-furyl)acryloyl]-Gly-L-Leu amide
thermolysinvariant S53D/L155A	Bacillus thermoproteolyticus	N-carbobenzoxy-L-Asp-L-Phe methyl ester	6.4	0.69	9.2	pH 7.5, 25 °C	PMID 20214932	Open record: thermolysin variant S53D/L155A, N-carbobenzoxy-L-Asp-L-Phe methyl ester
thermolysinvariant S53D/L155A/G8C/N60C/S65P	Bacillus thermoproteolyticus	N-[3-(2-furyl)acryloyl]-Gly-L-Leu amide0 M NaCl	—	—	23	pH 7.5, 25 °C	PMID 20214932	Open record: thermolysin variant S53D/L155A/G8C/N60C/S65P, N-[3-(2-furyl)acryloyl]-Gly-L-Leu amide
thermolysinvariant S53D/L155A/G8C/N60C/S65P	Bacillus thermoproteolyticus	N-[3-(2-furyl)acryloyl]-Gly-L-Leu amide4 M NaCl	—	—	330	pH 7.5, 25 °C	PMID 20214932	Open record: thermolysin variant S53D/L155A/G8C/N60C/S65P, N-[3-(2-furyl)acryloyl]-Gly-L-Leu amide
thermolysinvariant S53D/L155A/G8C/N60C/S65P	Bacillus thermoproteolyticus	N-carbobenzoxy-L-Asp-L-Phe methyl ester	3.3	0.55	6	pH 7.5, 25 °C	PMID 20214932	Open record: thermolysin variant S53D/L155A/G8C/N60C/S65P, N-carbobenzoxy-L-Asp-L-Phe methyl ester
thermolysinvariant Y157F	Bacillus thermoproteolyticus	tyrosyl-glycyl-glycyl-phenylalanyl-leucine	1.67	0.48	3.5		PMID 9827548	Open record: thermolysin variant Y157F, tyrosyl-glycyl-glycyl-phenylalanyl-leucine