Values CatLog read from this paper
Additional data about thermolysin specificity in buffer- and glycerol-containing media
PMID 9003446doi:10.1016/s0167-4838(96)00142-2Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 12 records (34 values) from this paper: thermolysin from Bacillus thermoproteolyticus.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 2 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| thermolysin | Bacillus thermoproteolyticus | H-Gly-Phe-Ala-OHno glycerol | 0.06 | 0.3 | 0.2 | pH 8, 37 °C | Open record: thermolysin, H-Gly-Phe-Ala-OH |
| thermolysin | Bacillus thermoproteolyticus | H-Gly-Phe-Ala-OH5 M glycerol | 0.077 | 0.6 | 0.128 | pH 8, 37 °C | Open record: thermolysin, H-Gly-Phe-Ala-OH |
| thermolysin | Bacillus thermoproteolyticus | H-Gly-Phe-Leu-OHno glycerol | 0.045 | 0.4 | 0.113 | pH 8, 37 °C | Open record: thermolysin, H-Gly-Phe-Leu-OH |
| thermolysin | Bacillus thermoproteolyticus | H-Gly-Phe-Leu-OH5 M glycerol | 0.027 | 1.8 | 0.015 calc. | pH 8, 37 °C | Open record: thermolysin, H-Gly-Phe-Leu-OH |
| thermolysin | Bacillus thermoproteolyticus | H-Gly-Phe-Leu-OHno glycerol | 0.013 | 0.7 | 0.018 | pH 8, 37 °C | Open record: thermolysin, H-Gly-Phe-Leu-OH |
| thermolysin | Bacillus thermoproteolyticus | H-Gly-Phe-Leu-OH5 M glycerol | 0.0067 | 2.5 | 0.0033 | pH 8, 37 °C | Open record: thermolysin, H-Gly-Phe-Leu-OH |
| thermolysin | Bacillus thermoproteolyticus | H-Gly-Phe-Ser-Ala-OHno glycerol | 0.3 | 1.2 | 0.25 | pH 8, 37 °C | Open record: thermolysin, H-Gly-Phe-Ser-Ala-OH |
| thermolysin | Bacillus thermoproteolyticus | H-Gly-Phe-Ser-Ala-OH5 M glycerol | 0.592 | 12 | 0.05 | pH 8, 37 °C | Open record: thermolysin, H-Gly-Phe-Ser-Ala-OH |
| thermolysin | Bacillus thermoproteolyticus | H-Gly-Phe-Ser-Ala-OHno glycerol | 0.07 | 0.7 | 0.1 | pH 8, 37 °C | Open record: thermolysin, H-Gly-Phe-Ser-Ala-OH |
| thermolysin | Bacillus thermoproteolyticus | H-Gly-Phe-Ser-Ala-OH5 M glycerol | 0.173 | 6.8 | 0.025 | pH 8, 37 °C | Open record: thermolysin, H-Gly-Phe-Ser-Ala-OH |
| thermolysin | Bacillus thermoproteolyticus | H-Gly-Phe-Ser-OHno glycerol | 0.035 | 1.3 | 0.027 | pH 8, 37 °C | Open record: thermolysin, H-Gly-Phe-Ser-OH |
| thermolysin | Bacillus thermoproteolyticus | H-Gly-Phe-Ser-OH5 M glycerol | 0.05 | 3.5 | 0.014 calc. | pH 8, 37 °C | Open record: thermolysin, H-Gly-Phe-Ser-OH |