Values CatLog read from this paper
Thermolysin: kinetic study with oligopeptides
PMID 4993757doi:10.1111/j.1432-1033.1970.tb01018.xOpen in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 40 records (118 values) from this paper: thermolysin from Bacillus thermoproteolyticus.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 2 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| thermolysin | Bacillus thermoproteolyticus | L-phenylalanylglycyl-glycyl-L-leucyl-L-alanine | 150.6 | 4.8 | 50.2 | pH 7, 40 °C | Open record: thermolysin, L-phenylalanylglycyl-glycyl-L-leucyl-L-alanine |
| thermolysin | Bacillus thermoproteolyticus | L-phenylalanylglycyl-L-leucyl-L-alanine | 53 | 4.6 | 11.5 | pH 7, 40 °C | Open record: thermolysin, L-phenylalanylglycyl-L-leucyl-L-alanine |
| thermolysin | Bacillus thermoproteolyticus | N-benzyloxycarbonyl-D-alanylglycyl-glycyl-L-leucyl-L-alanine | 127.5 | 7.1 | 18 | pH 7, 40 °C | Open record: thermolysin, N-benzyloxycarbonyl-D-alanylglycyl-glycyl-L-leucyl-L-alanine |
| thermolysin | Bacillus thermoproteolyticus | N-benzyloxycarbonyl-D-alanylglycyl-L-leucyl-L-alanine | 625 | 16 | 39 | pH 7, 40 °C | Open record: thermolysin, N-benzyloxycarbonyl-D-alanylglycyl-L-leucyl-L-alanine |
| thermolysin | Bacillus thermoproteolyticus | N-benzyloxycarbonyl-L-alanyl-L-leucyl-L-alanine | 996.9 | 4.9 | 203.4 | pH 7, 40 °C | Open record: thermolysin, N-benzyloxycarbonyl-L-alanyl-L-leucyl-L-alanine |
| thermolysin | Bacillus thermoproteolyticus | N-benzyloxycarbonyl-L-alanylglycyl-glycyl-L-leucyl-L-alanine | 50 | 7.5 | 6.6 | pH 7, 40 °C | Open record: thermolysin, N-benzyloxycarbonyl-L-alanylglycyl-glycyl-L-leucyl-L-alanine |
| thermolysin | Bacillus thermoproteolyticus | N-benzyloxycarbonyl-L-alanylglycyl-L-leucyl-L-alanine | 5208 | 8.6 | 605.6 | pH 7, 40 °C | Open record: thermolysin, N-benzyloxycarbonyl-L-alanylglycyl-L-leucyl-L-alanine |
| thermolysin | Bacillus thermoproteolyticus | N-benzyloxycarbonyl-L-phenylalanyl-L-alanyl-L-alanine | 21.9 | 2.6 | 8.4 | pH 7, 40 °C | Open record: thermolysin, N-benzyloxycarbonyl-L-phenylalanyl-L-alanyl-L-alanine |
| thermolysin | Bacillus thermoproteolyticus | N-benzyloxycarbonyl-L-phenylalanyl-L-isoleucyl-L-alanine | 148 | 0.82 | 205.5 | pH 7, 40 °C | Open record: thermolysin, N-benzyloxycarbonyl-L-phenylalanyl-L-isoleucyl-L-alanine |
| thermolysin | Bacillus thermoproteolyticus | N-benzyloxycarbonyl-L-phenylalanyl-L-leucyl-L-alanineinitial substrate concentrations up to approx. 25 mM | 398.3 | 0.58 | 678 | pH 7, 40 °C | Open record: thermolysin, N-benzyloxycarbonyl-L-phenylalanyl-L-leucyl-L-alanine |
| thermolysin | Bacillus thermoproteolyticus | N-benzyloxycarbonyl-L-phenylalanyl-L-leucyl-L-alanine1.7% ethanol | 416 | 0.72 | 577.8 | pH 7, 40 °C | Open record: thermolysin, N-benzyloxycarbonyl-L-phenylalanyl-L-leucyl-L-alanine |
| thermolysin | Bacillus thermoproteolyticus | N-benzyloxycarbonyl-L-phenylalanyl-L-phenylalanyl-L-alanine | 162 | 0.45 | 360 | pH 7, 40 °C | Open record: thermolysin, N-benzyloxycarbonyl-L-phenylalanyl-L-phenylalanyl-L-alanine |
| thermolysin | Bacillus thermoproteolyticus | N-benzyloxycarbonyl-L-phenylalanyl-L-seryl-L-alanine | 3 | 1.6 | 1.9 | pH 7, 40 °C | Open record: thermolysin, N-benzyloxycarbonyl-L-phenylalanyl-L-seryl-L-alanine |
| thermolysin | Bacillus thermoproteolyticus | N-benzyloxycarbonyl-L-phenylalanyl-L-tyrosyl-L-alanine | 7.1 | 0.72 | 9.9 | pH 7, 40 °C | Open record: thermolysin, N-benzyloxycarbonyl-L-phenylalanyl-L-tyrosyl-L-alanine |
| thermolysin | Bacillus thermoproteolyticus | N-benzyloxycarbonyl-L-phenylalanyl-L-valyl-L-alanine | 169 | 0.78 | 217 | pH 7, 40 °C | Open record: thermolysin, N-benzyloxycarbonyl-L-phenylalanyl-L-valyl-L-alanine |
| thermolysin | Bacillus thermoproteolyticus | N-benzyloxycarbonyl-L-phenylalanylglycyl-glycyl-L-leucyl-L-alanine | 21.9 | 3 | 7.3 | pH 7, 40 °C | Open record: thermolysin, N-benzyloxycarbonyl-L-phenylalanylglycyl-glycyl-L-leucyl-L-alanine |
| thermolysin | Bacillus thermoproteolyticus | N-benzyloxycarbonyl-L-phenylalanylglycyl-L-alanine | 7.8 | 7.4 | 1.05 | pH 7, 40 °C | Open record: thermolysin, N-benzyloxycarbonyl-L-phenylalanylglycyl-L-alanine |
| thermolysin | Bacillus thermoproteolyticus | N-benzyloxycarbonyl-L-phenylalanylglycyl-L-leucyl-L-alanine | 446.4 | 0.91 | 490.5 | pH 7, 40 °C | Open record: thermolysin, N-benzyloxycarbonyl-L-phenylalanylglycyl-L-leucyl-L-alanine |
| thermolysin | Bacillus thermoproteolyticus | N-benzyloxycarbonyl-L-tyrosyl-glycinamide | 0.125 | 20.8 | 0.006 | pH 7, 40 °C | Open record: thermolysin, N-benzyloxycarbonyl-L-tyrosyl-glycinamide |
| thermolysin | Bacillus thermoproteolyticus | N-benzyloxycarbonyl-L-tyrosyl-L-serinamide | 0.36 | 2.3 | 0.16 | pH 7, 40 °C | Open record: thermolysin, N-benzyloxycarbonyl-L-tyrosyl-L-serinamide |
| thermolysin | Bacillus thermoproteolyticus | N-benzyloxycarbonylglycyl-L-alaninamide | 44.5 | 600 | 0.074 | pH 7, 40 °C | Open record: thermolysin, N-benzyloxycarbonylglycyl-L-alaninamide |
| thermolysin | Bacillus thermoproteolyticus | N-benzyloxycarbonylglycyl-L-isoleucinamide | 59.3 | 33.3 | 1.8 | pH 7, 40 °C | Open record: thermolysin, N-benzyloxycarbonylglycyl-L-isoleucinamide |
| thermolysin | Bacillus thermoproteolyticus | N-benzyloxycarbonylglycyl-L-leucinamideinitial substrate concentrations up to approx. 25 mM | 105 | 20.6 | 5.1 | pH 7, 40 °C | Open record: thermolysin, N-benzyloxycarbonylglycyl-L-leucinamide |
| thermolysin | Bacillus thermoproteolyticus | N-benzyloxycarbonylglycyl-L-leucinamide1.7% ethanol | 89 | 33.3 | 2.7 | pH 7, 40 °C | Open record: thermolysin, N-benzyloxycarbonylglycyl-L-leucinamide |
| thermolysin | Bacillus thermoproteolyticus | N-benzyloxycarbonylglycyl-L-leucyl-D-alanine | 5.2 | 16.6 | 0.3 | pH 7, 40 °C | Open record: thermolysin, N-benzyloxycarbonylglycyl-L-leucyl-D-alanine |
| thermolysin | Bacillus thermoproteolyticus | N-benzyloxycarbonylglycyl-L-leucyl-L-alanine | 780.6 | 10.6 | 78.1 | pH 7, 40 °C | Open record: thermolysin, N-benzyloxycarbonylglycyl-L-leucyl-L-alanine |
| thermolysin | Bacillus thermoproteolyticus | N-benzyloxycarbonylglycyl-L-leucyl-L-leucine | 374 | 2.6 | 143.9 | pH 7, 40 °C | Open record: thermolysin, N-benzyloxycarbonylglycyl-L-leucyl-L-leucine |
| thermolysin | Bacillus thermoproteolyticus | N-benzyloxycarbonylglycyl-L-leucyl-L-phenylalanine | 120 | 2.4 | 50 | pH 7, 40 °C | Open record: thermolysin, N-benzyloxycarbonylglycyl-L-leucyl-L-phenylalanine |
| thermolysin | Bacillus thermoproteolyticus | N-benzyloxycarbonylglycyl-L-leucylglycine | 65.4 | 10.8 | 6.1 | pH 7, 40 °C | Open record: thermolysin, N-benzyloxycarbonylglycyl-L-leucylglycine |
| thermolysin | Bacillus thermoproteolyticus | N-benzyloxycarbonylglycyl-L-leucylglycyl-D-alanine | 25 | 13.4 | 1.9 | pH 7, 40 °C | Open record: thermolysin, N-benzyloxycarbonylglycyl-L-leucylglycyl-D-alanine |
| thermolysin | Bacillus thermoproteolyticus | N-benzyloxycarbonylglycyl-L-leucylglycyl-glycine | 62.4 | 11.8 | 5.3 | pH 7, 40 °C | Open record: thermolysin, N-benzyloxycarbonylglycyl-L-leucylglycyl-glycine |
| thermolysin | Bacillus thermoproteolyticus | N-benzyloxycarbonylglycyl-L-leucylglycyl-L-alanine | 418.9 | 13.6 | 30.8 | pH 7, 40 °C | Open record: thermolysin, N-benzyloxycarbonylglycyl-L-leucylglycyl-L-alanine |
| thermolysin | Bacillus thermoproteolyticus | N-benzyloxycarbonylglycyl-L-leucylglycyl-L-phenylalanine | 131.6 | 11 | 12 | pH 7, 40 °C | Open record: thermolysin, N-benzyloxycarbonylglycyl-L-leucylglycyl-L-phenylalanine |
| thermolysin | Bacillus thermoproteolyticus | N-benzyloxycarbonylglycyl-L-phenylalaninamide | 38.5 | 14.7 | 2.6 | pH 7, 40 °C | Open record: thermolysin, N-benzyloxycarbonylglycyl-L-phenylalaninamide |
| thermolysin | Bacillus thermoproteolyticus | N-benzyloxycarbonylglycyl-L-prolyl-L-leucylglycine | 11.2 | 12.8 | 0.875 calc. | pH 7, 40 °C | Open record: thermolysin, N-benzyloxycarbonylglycyl-L-prolyl-L-leucylglycine |
| thermolysin | Bacillus thermoproteolyticus | N-benzyloxycarbonylglycyl-L-prolyl-L-leucylglycyl-L-proline | 41.6 | 13.3 | 3.13 calc. | pH 7, 40 °C | Open record: thermolysin, N-benzyloxycarbonylglycyl-L-prolyl-L-leucylglycyl-L-proline |
| thermolysin | Bacillus thermoproteolyticus | N-benzyloxycarbonylglycyl-L-serinamide | 1.6 | 600 | 0.003 | pH 7, 40 °C | Open record: thermolysin, N-benzyloxycarbonylglycyl-L-serinamide |
| thermolysin | Bacillus thermoproteolyticus | N-benzyloxycarbonylglycyl-L-tyrosinamide | 1.6 | 14.7 | 0.11 | pH 7, 40 °C | Open record: thermolysin, N-benzyloxycarbonylglycyl-L-tyrosinamide |
| thermolysin | Bacillus thermoproteolyticus | N-benzyloxycarbonylglycyl-L-valinamide | 22.6 | 71.5 | 0.32 | pH 7, 40 °C | Open record: thermolysin, N-benzyloxycarbonylglycyl-L-valinamide |
| thermolysin | Bacillus thermoproteolyticus | N-benzyloxycarbonylglycylglycyl-L-leucyl-L-alanine | 362 | 12.2 | 29.7 | pH 7, 40 °C | Open record: thermolysin, N-benzyloxycarbonylglycylglycyl-L-leucyl-L-alanine |