Values CatLog read from this paper
Effects of site-directed mutagenesis of Asn116 in the β-hairpin of the N-terminal domain of thermolysin on its activity and stability
PMID 22648563doi:10.1093/jb/mvs064Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 25 records (35 values) from this paper: thermolysin from Bacillus thermoproteolyticus.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| thermolysin | Bacillus thermoproteolyticus | N-[3-(2-furyl)acryloyl]-glycyl-L-leucine amidepH dependence in 40 mM acetate-NaOH (pH 4.0-5.5) | — | — | 37 | 25 °C | Open record: thermolysin, N-[3-(2-furyl)acryloyl]-glycyl-L-leucine amide |
| thermolysin | Bacillus thermoproteolyticus | N-[3-(2-furyl)acryloyl]-glycyl-L-leucine amidewithout NaCl | — | — | 30 | pH 7.5, 25 °C | Open record: thermolysin, N-[3-(2-furyl)acryloyl]-glycyl-L-leucine amide |
| thermolysin | Bacillus thermoproteolyticus | N-[3-(2-furyl)acryloyl]-glycyl-L-leucine amidewith 4 M NaCl | — | — | 440 | pH 7.5, 25 °C | Open record: thermolysin, N-[3-(2-furyl)acryloyl]-glycyl-L-leucine amide |
| thermolysin | Bacillus thermoproteolyticus | N-[3-(2-furyl)acryloyl]-glycyl-L-leucine amide | — | — | 29 | 25 °C | Open record: thermolysin, N-[3-(2-furyl)acryloyl]-glycyl-L-leucine amide |
| thermolysin | Bacillus thermoproteolyticus | N-carbobenzoxy-L-aspartyl-L-phenylalanine methyl ester | 7.7 | 0.39 | 20 | pH 7.5, 25 °C | Open record: thermolysin, N-carbobenzoxy-L-aspartyl-L-phenylalanine methyl ester |
| thermolysinvariant N116A | Bacillus thermoproteolyticus | N-[3-(2-furyl)acryloyl]-glycyl-L-leucine amidepH dependence in 40 mM acetate-NaOH (pH 4.0-5.5) | — | — | 31 | 25 °C | Open record: thermolysin variant N116A, N-[3-(2-furyl)acryloyl]-glycyl-L-leucine amide |
| thermolysinvariant N116A | Bacillus thermoproteolyticus | N-[3-(2-furyl)acryloyl]-glycyl-L-leucine amidewithout NaCl | — | — | 24 | pH 7.5, 25 °C | Open record: thermolysin variant N116A, N-[3-(2-furyl)acryloyl]-glycyl-L-leucine amide |
| thermolysinvariant N116A | Bacillus thermoproteolyticus | N-[3-(2-furyl)acryloyl]-glycyl-L-leucine amidewith 4 M NaCl | — | — | 330 | pH 7.5, 25 °C | Open record: thermolysin variant N116A, N-[3-(2-furyl)acryloyl]-glycyl-L-leucine amide |
| thermolysinvariant N116A | Bacillus thermoproteolyticus | N-[3-(2-furyl)acryloyl]-glycyl-L-leucine amide | — | — | 23 | 25 °C | Open record: thermolysin variant N116A, N-[3-(2-furyl)acryloyl]-glycyl-L-leucine amide |
| thermolysinvariant N116A | Bacillus thermoproteolyticus | N-carbobenzoxy-L-aspartyl-L-phenylalanine methyl ester | 6.7 | 0.41 | 16 | pH 7.5, 25 °C | Open record: thermolysin variant N116A, N-carbobenzoxy-L-aspartyl-L-phenylalanine methyl ester |
| thermolysinvariant N116D | Bacillus thermoproteolyticus | N-[3-(2-furyl)acryloyl]-glycyl-L-leucine amidepH dependence in 40 mM acetate-NaOH (pH 4.0-5.5) | — | — | 117 | 25 °C | Open record: thermolysin variant N116D, N-[3-(2-furyl)acryloyl]-glycyl-L-leucine amide |
| thermolysinvariant N116D | Bacillus thermoproteolyticus | N-[3-(2-furyl)acryloyl]-glycyl-L-leucine amidewithout NaCl | — | — | 95 | pH 7.5, 25 °C | Open record: thermolysin variant N116D, N-[3-(2-furyl)acryloyl]-glycyl-L-leucine amide |
| thermolysinvariant N116D | Bacillus thermoproteolyticus | N-[3-(2-furyl)acryloyl]-glycyl-L-leucine amidewith 4 M NaCl | — | — | 1 × 10³ | pH 7.5, 25 °C | Open record: thermolysin variant N116D, N-[3-(2-furyl)acryloyl]-glycyl-L-leucine amide |
| thermolysinvariant N116D | Bacillus thermoproteolyticus | N-[3-(2-furyl)acryloyl]-glycyl-L-leucine amide | — | — | 95 | 25 °C | Open record: thermolysin variant N116D, N-[3-(2-furyl)acryloyl]-glycyl-L-leucine amide |
| thermolysinvariant N116D | Bacillus thermoproteolyticus | N-carbobenzoxy-L-aspartyl-L-phenylalanine methyl ester | 12 | 0.43 | 27 | pH 7.5, 25 °C | Open record: thermolysin variant N116D, N-carbobenzoxy-L-aspartyl-L-phenylalanine methyl ester |
| thermolysinvariant N116Q | Bacillus thermoproteolyticus | N-[3-(2-furyl)acryloyl]-glycyl-L-leucine amidepH dependence in 40 mM acetate-NaOH (pH 4.0-5.5) | — | — | 37 | 25 °C | Open record: thermolysin variant N116Q, N-[3-(2-furyl)acryloyl]-glycyl-L-leucine amide |
| thermolysinvariant N116Q | Bacillus thermoproteolyticus | N-[3-(2-furyl)acryloyl]-glycyl-L-leucine amidewithout NaCl | — | — | 31 | pH 7.5, 25 °C | Open record: thermolysin variant N116Q, N-[3-(2-furyl)acryloyl]-glycyl-L-leucine amide |
| thermolysinvariant N116Q | Bacillus thermoproteolyticus | N-[3-(2-furyl)acryloyl]-glycyl-L-leucine amidewith 4 M NaCl | — | — | 270 | pH 7.5, 25 °C | Open record: thermolysin variant N116Q, N-[3-(2-furyl)acryloyl]-glycyl-L-leucine amide |
| thermolysinvariant N116Q | Bacillus thermoproteolyticus | N-[3-(2-furyl)acryloyl]-glycyl-L-leucine amide | — | — | 29 | 25 °C | Open record: thermolysin variant N116Q, N-[3-(2-furyl)acryloyl]-glycyl-L-leucine amide |
| thermolysinvariant N116Q | Bacillus thermoproteolyticus | N-carbobenzoxy-L-aspartyl-L-phenylalanine methyl ester | 7.1 | 0.38 | 19 | pH 7.5, 25 °C | Open record: thermolysin variant N116Q, N-carbobenzoxy-L-aspartyl-L-phenylalanine methyl ester |
| thermolysinvariant N116T | Bacillus thermoproteolyticus | N-[3-(2-furyl)acryloyl]-glycyl-L-leucine amidepH dependence in 40 mM acetate-NaOH (pH 4.0-5.5) | — | — | 26 | 25 °C | Open record: thermolysin variant N116T, N-[3-(2-furyl)acryloyl]-glycyl-L-leucine amide |
| thermolysinvariant N116T | Bacillus thermoproteolyticus | N-[3-(2-furyl)acryloyl]-glycyl-L-leucine amidewithout NaCl | — | — | 24 | pH 7.5, 25 °C | Open record: thermolysin variant N116T, N-[3-(2-furyl)acryloyl]-glycyl-L-leucine amide |
| thermolysinvariant N116T | Bacillus thermoproteolyticus | N-[3-(2-furyl)acryloyl]-glycyl-L-leucine amidewith 4 M NaCl | — | — | 240 | pH 7.5, 25 °C | Open record: thermolysin variant N116T, N-[3-(2-furyl)acryloyl]-glycyl-L-leucine amide |
| thermolysinvariant N116T | Bacillus thermoproteolyticus | N-[3-(2-furyl)acryloyl]-glycyl-L-leucine amide | — | — | 23 | 25 °C | Open record: thermolysin variant N116T, N-[3-(2-furyl)acryloyl]-glycyl-L-leucine amide |
| thermolysinvariant N116T | Bacillus thermoproteolyticus | N-carbobenzoxy-L-aspartyl-L-phenylalanine methyl ester | 5.4 | 0.49 | 11 | pH 7.5, 25 °C | Open record: thermolysin variant N116T, N-carbobenzoxy-L-aspartyl-L-phenylalanine methyl ester |