CatLogenzyme kinetics catalog

Values CatLog read from this paper

Differences in transition state stabilization between thermolysin (EC 3.4.24.27) and neprilysin (EC 3.4.24.11)

PMID 9827548doi:10.1016/s0014-5793(98)01267-8Open in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 6 records (18 values) from this paper: neprilysin, thermolysin from Bacillus thermoproteolyticus.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.

EC 3.4.24.11, EC 3.4.24.27

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)Conditions
neprilysin—tyrosyl-D-alanyl-glycyl-phenylalanyl-leucine19.150.055348Open
neprilysinvariant D709A—tyrosyl-D-alanyl-glycyl-phenylalanyl-leucine0.060.0471.2Open
neprilysinvariant Y625F—tyrosyl-D-alanyl-glycyl-phenylalanyl-leucine16.720.141118Open
thermolysinBacillus thermoproteolyticustyrosyl-glycyl-glycyl-phenylalanyl-leucine155.50.21740Open
thermolysinvariant D226ABacillus thermoproteolyticustyrosyl-glycyl-glycyl-phenylalanyl-leucine37.531.98518.8Open
thermolysinvariant Y157FBacillus thermoproteolyticustyrosyl-glycyl-glycyl-phenylalanyl-leucine1.670.483.5Open