EC 3.4.24.11: neprilysin

HydrolasesOpen in Browse

CatLog read 124 records for EC 3.4.24.11 from 6 papers, for 3 organisms. Names in these papers: neprilysin (neutral endopeptidase, NEP), neprilysin (NEP), neprilysin 2 (NEP2), secreted isoform NEP2(s), neutral endopeptidase (NEP, neprilysin) and 2 more.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units.

CatLog also holds 28 records for EC 3.4.24.11 that are not yet re-read: from its earlier version, built from the enzyme databases and partly checked against the papers. All records for EC 3.4.24.11 in Browse.

Papers

Papers with records for EC 3.4.24.11
Paper	Organisms	Records
Engineering neprilysin activity and specificity to create a novel therapeutic for Alzheimer's disease	Homo sapiens	46
Cell-specific activity of neprilysin 2 isoforms and enzymic specificity compared with neprilysin	Homo sapiens, Rattus norvegicus	45
Heterologous expression and characterization of recombinant Lactococcus lactis neutral endopeptidase (neprilysin)	Lactococcus lactis subsp. cremoris	19
A three-dimensional model of the neprilysin 2 active site based on the X-ray structure of neprilysin. Identification of residues involved in substrate hydrolysis and inhibitor binding of neprilysin…	Rattus norvegicus	6
Selective neurotensin-derived internally quenched fluorogenic substrates for neurolysin (EC 3.4.24.16): comparison with thimet oligopeptidase (EC 3.4.24.15) and neprilysin (EC 3.4.24.11)	—	5
Differences in transition state stabilization between thermolysin (EC 3.4.24.27) and neprilysin (EC 3.4.24.11)	—	3

Records

Records for EC 3.4.24.11 read from the paper
Enzyme	Organism	Substrate	k_cat (s⁻¹)	K_m (mM)	k_cat/K_m (mM⁻¹ s⁻¹)	Conditions	Paper	Open record
neprilysin	—	tyrosyl-D-alanyl-glycyl-phenylalanyl-leucine	19.15	0.055	348		PMID 9827548	Open record: neprilysin, tyrosyl-D-alanyl-glycyl-phenylalanyl-leucine
neprilysinvariant D709A	—	tyrosyl-D-alanyl-glycyl-phenylalanyl-leucine	0.06	0.047	1.2		PMID 9827548	Open record: neprilysin variant D709A, tyrosyl-D-alanyl-glycyl-phenylalanyl-leucine
neprilysinvariant Y625F	—	tyrosyl-D-alanyl-glycyl-phenylalanyl-leucine	16.72	0.141	118		PMID 9827548	Open record: neprilysin variant Y625F, tyrosyl-D-alanyl-glycyl-phenylalanyl-leucine
neprilysin (NEP)	—	ortho-aminobenzoyl-Glu-Asn-Lys-Pro-Arg-Arg-Pro-Tyr-Gln-N-(2,4-dinitrophenyl)ethylenediamine	3	—	—	pH 8, 37 °C	PMID 11355859	Open record: neprilysin (NEP), ortho-aminobenzoyl-Glu-Asn-Lys-Pro-Arg-Arg-Pro-Tyr-Gln-N-(2,4-dinitrophenyl)ethylenediamine
neprilysin (NEP)	—	ortho-aminobenzoyl-Glu-Asn-Lys-Pro-Arg-Arg-Pro-Tyr-Ile-Gln-N-(2,4-dinitrophenyl)ethylenediamine	1.4	—	—	pH 8, 37 °C	PMID 11355859	Open record: neprilysin (NEP), ortho-aminobenzoyl-Glu-Asn-Lys-Pro-Arg-Arg-Pro-Tyr-Ile-Gln-N-(2,4-dinitrophenyl)ethylenediamine
neprilysin (NEP)	—	ortho-aminobenzoyl-Glu-Asn-Lys-Pro-Arg-Arg-Pro-Tyr-Ile-Leu-Gln-N-(2,4-dinitrophenyl)ethylenediamine	12	—	—	pH 8, 37 °C	PMID 11355859	Open record: neprilysin (NEP), ortho-aminobenzoyl-Glu-Asn-Lys-Pro-Arg-Arg-Pro-Tyr-Ile-Leu-Gln-N-(2,4-dinitrophenyl)ethylenediamine
neprilysin (NEP)	—	ortho-aminobenzoyl-Leu-Tyr-Glu-Asn-Lys-Pro-Arg-Arg-Pro-Tyr-Ile-Leu-Gln-N-(2,4-dinitrophenyl)ethylenediamine	0.8	—	—	pH 8, 37 °C	PMID 11355859	Open record: neprilysin (NEP), ortho-aminobenzoyl-Leu-Tyr-Glu-Asn-Lys-Pro-Arg-Arg-Pro-Tyr-Ile-Leu-Gln-N-(2,4-dinitrophenyl)ethylenediamine
neprilysin (NEP)	—	ortho-aminobenzoyl-Lys-Pro-Arg-Arg-Pro-Tyr-Ile-Leu-Gln-N-(2,4-dinitrophenyl)ethylenediamine	0.3	—	—	pH 8, 37 °C	PMID 11355859	Open record: neprilysin (NEP), ortho-aminobenzoyl-Lys-Pro-Arg-Arg-Pro-Tyr-Ile-Leu-Gln-N-(2,4-dinitrophenyl)ethylenediamine
neprilysin (NEP)	Homo sapiens	3-methoxysuccinyl-Gly-Leu-Phe-7-amido-4-methylcoumarin	22	0.08	270	37 °C	PMID 11964170	Open record: neprilysin (NEP), 3-methoxysuccinyl-Gly-Leu-Phe-7-amido-4-methylcoumarin
neprilysin (NEP)	Homo sapiens	Arg-Pro-Lys-Pro-Gln-Gln-Phe-Phe-Gly-Leu-Met-NH2	13	—	—	pH 7.2, 37 °C	PMID 11964170	Open record: neprilysin (NEP), Arg-Pro-Lys-Pro-Gln-Gln-Phe-Phe-Gly-Leu-Met-NH2
neprilysin (NEP)	Homo sapiens	Arg-Pro-Pro-Gly-Phe-Ser-Pro-Phe-Arg	25	—	—	pH 7.2, 37 °C	PMID 11964170	Open record: neprilysin (NEP), Arg-Pro-Pro-Gly-Phe-Ser-Pro-Phe-Arg
neprilysin (NEP)	Homo sapiens	Asp-Arg-Val-Tyr-Ile-His-Pro-Phe-His-Leu	7	—	—	pH 7.2, 37 °C	PMID 11964170	Open record: neprilysin (NEP), Asp-Arg-Val-Tyr-Ile-His-Pro-Phe-His-Leu
neprilysin (NEP)	Homo sapiens	Asp-Tyr-Met-Gly-Trp-Met	23	—	—	pH 7.2, 37 °C	PMID 11964170	Open record: neprilysin (NEP), Asp-Tyr-Met-Gly-Trp-Met
neprilysin (NEP)	Homo sapiens	Asp-Tyr-Met-Gly-Trp-Met-Asp-Phe-NH2	12.5	—	—	pH 7.2, 37 °C	PMID 11964170	Open record: neprilysin (NEP), Asp-Tyr-Met-Gly-Trp-Met-Asp-Phe-NH2
neprilysin (NEP)	Homo sapiens	CCK-8 (sulphated)	8.8	—	—	pH 7.2, 37 °C	PMID 11964170	Open record: neprilysin (NEP), CCK-8 (sulphated)
neprilysin (NEP)	Homo sapiens	Gly-Gly-Phe-Met	17	—	—	pH 7.2, 37 °C	PMID 11964170	Open record: neprilysin (NEP), Gly-Gly-Phe-Met
neprilysin (NEP)	Homo sapiens	Gly-Trp-Met-Asp-Phe-NH2	8.3	—	—	pH 7.2, 37 °C	PMID 11964170	Open record: neprilysin (NEP), Gly-Trp-Met-Asp-Phe-NH2
neprilysin (NEP)	Homo sapiens	pGlu-His-Trp-Ser-Tyr-Gly-Leu-Arg-Pro-Gly-NH2	2	—	—	pH 7.2, 37 °C	PMID 11964170	Open record: neprilysin (NEP), pGlu-His-Trp-Ser-Tyr-Gly-Leu-Arg-Pro-Gly-NH2
neprilysin (NEP)	Homo sapiens	Phe-Gly-Leu-Met-NH2	12	—	—	pH 7.2, 37 °C	PMID 11964170	Open record: neprilysin (NEP), Phe-Gly-Leu-Met-NH2
neprilysin (NEP)	Homo sapiens	Phe-Phe-Gly-Leu-Met-NH2	30	—	—	pH 7.2, 37 °C	PMID 11964170	Open record: neprilysin (NEP), Phe-Phe-Gly-Leu-Met-NH2
neprilysin (NEP)	Homo sapiens	rANP-(1-28)	6.7	—	—	pH 7.2, 37 °C	PMID 11964170	Open record: neprilysin (NEP), rANP-(1-28)
neprilysin (NEP)	Homo sapiens	succinyl-Ala-Ala-Phe-7-amido-4-methylcoumarin	37	0.07	523	37 °C	PMID 11964170	Open record: neprilysin (NEP), succinyl-Ala-Ala-Phe-7-amido-4-methylcoumarin
neprilysin (NEP)	Homo sapiens	succinyl-Ala-Ala-Val-7-amido-4-methylcoumarin	23	0.1	230	37 °C	PMID 11964170	Open record: neprilysin (NEP), succinyl-Ala-Ala-Val-7-amido-4-methylcoumarin
neprilysin (NEP)	Homo sapiens	Trp-Met-Asp-Phe-NH2	6.7	—	—	pH 7.2, 37 °C	PMID 11964170	Open record: neprilysin (NEP), Trp-Met-Asp-Phe-NH2
neprilysin (NEP)	Homo sapiens	Tyr-Gly-Ala-Phe-Met	27	—	—	pH 7.2, 37 °C	PMID 11964170	Open record: neprilysin (NEP), Tyr-Gly-Ala-Phe-Met
neprilysin (NEP)	Homo sapiens	Tyr-Gly-Gly-Phe-Leu	20	—	—	pH 7.2, 37 °C	PMID 11964170	Open record: neprilysin (NEP), Tyr-Gly-Gly-Phe-Leu
neprilysin (NEP)	Homo sapiens	Tyr-Gly-Gly-Phe-Leu-NH2	3.3	—	—	pH 7.2, 37 °C	PMID 11964170	Open record: neprilysin (NEP), Tyr-Gly-Gly-Phe-Leu-NH2
neprilysin (NEP)	Homo sapiens	Tyr-Gly-Gly-Phe-Met	22	—	—	pH 7.2, 37 °C	PMID 11964170	Open record: neprilysin (NEP), Tyr-Gly-Gly-Phe-Met
neprilysin (NEP)	Homo sapiens	Tyr-Gly-Gly-Phe-Met-NH2	13	—	—	pH 7.2, 37 °C	PMID 11964170	Open record: neprilysin (NEP), Tyr-Gly-Gly-Phe-Met-NH2
neprilysin (neutral endopeptidase, NEP)	Homo sapiens	amyloid beta 1-40	0.83	0.104	8	pH 7.4, 37 °C	PMID 25089527	Open record: neprilysin (neutral endopeptidase, NEP), amyloid beta 1-40
neprilysin (neutral endopeptidase, NEP)	Homo sapiens	Angiotensin	8.2	0.0086	970	pH 7.4, 37 °C	PMID 25089527	Open record: neprilysin (neutral endopeptidase, NEP), Angiotensin
neprilysin (neutral endopeptidase, NEP)	Homo sapiens	Arg-vasopressin	2.8	0.0062	450	pH 7.4, 37 °C	PMID 25089527	Open record: neprilysin (neutral endopeptidase, NEP), Arg-vasopressin
neprilysin (neutral endopeptidase, NEP)	Homo sapiens	atrial natriuretic peptide	7.1	0.0084	910	pH 7.4, 37 °C	PMID 25089527	Open record: neprilysin (neutral endopeptidase, NEP), atrial natriuretic peptide
neprilysin (neutral endopeptidase, NEP)	Homo sapiens	Bradykinin	16	0.032	480	pH 7.4, 37 °C	PMID 25089527	Open record: neprilysin (neutral endopeptidase, NEP), Bradykinin
neprilysin (neutral endopeptidase, NEP)	Homo sapiens	Endothelin-1	0.68	0.053	13	37 °C	PMID 25089527	Open record: neprilysin (neutral endopeptidase, NEP), Endothelin-1
neprilysin (neutral endopeptidase, NEP)	Homo sapiens	gastrin-releasing peptide	26	0.15	170	pH 7.4, 37 °C	PMID 25089527	Open record: neprilysin (neutral endopeptidase, NEP), gastrin-releasing peptide
neprilysin (neutral endopeptidase, NEP)	Homo sapiens	Glucagon	4.5	0.025	180	pH 7.4, 37 °C	PMID 25089527	Open record: neprilysin (neutral endopeptidase, NEP), Glucagon
neprilysin (neutral endopeptidase, NEP)	Homo sapiens	glucagon-like peptide-1	3.3	0.024	140	pH 7.4, 37 °C	PMID 25089527	Open record: neprilysin (neutral endopeptidase, NEP), glucagon-like peptide-1
neprilysin (neutral endopeptidase, NEP)	Homo sapiens	Neurokinin A	17	0.0093	1.7 × 10³	pH 7.4, 37 °C	PMID 25089527	Open record: neprilysin (neutral endopeptidase, NEP), Neurokinin A
neprilysin (neutral endopeptidase, NEP)	Homo sapiens	Neurokinin B	20	0.022	920	pH 7.4, 37 °C	PMID 25089527	Open record: neprilysin (neutral endopeptidase, NEP), Neurokinin B
neprilysin (neutral endopeptidase, NEP)	Homo sapiens	Neurotensin	13	0.016	820	pH 7.4, 37 °C	PMID 25089527	Open record: neprilysin (neutral endopeptidase, NEP), Neurotensin
neprilysin (neutral endopeptidase, NEP)	Homo sapiens	Nociceptin	11	0.02	570	pH 7.4, 37 °C	PMID 25089527	Open record: neprilysin (neutral endopeptidase, NEP), Nociceptin
neprilysin (neutral endopeptidase, NEP)	Homo sapiens	Somatostatin 14	32	0.0074	4.4 × 10³	pH 7.4, 37 °C	PMID 25089527	Open record: neprilysin (neutral endopeptidase, NEP), Somatostatin 14
neprilysin (neutral endopeptidase, NEP)	Homo sapiens	Somatostatin 28	9.1	0.031	160	pH 7.4, 37 °C	PMID 25089527	Open record: neprilysin (neutral endopeptidase, NEP), Somatostatin 28
neprilysin (neutral endopeptidase, NEP)	Homo sapiens	Substance P	18	0.016	1.1 × 10³	pH 7.4, 37 °C	PMID 25089527	Open record: neprilysin (neutral endopeptidase, NEP), Substance P
neprilysin (neutral endopeptidase, NEP)	Homo sapiens	α-endorphin	8.9	0.024	390	pH 7.4, 37 °C	PMID 25089527	Open record: neprilysin (neutral endopeptidase, NEP), α-endorphin
neprilysin (neutral endopeptidase, NEP)	Homo sapiens	γ-endorphin	4.8	0.013	380	pH 7.4, 37 °C	PMID 25089527	Open record: neprilysin (neutral endopeptidase, NEP), γ-endorphin
neprilysin (neutral endopeptidase, NEP)variant G399V	Homo sapiens	amyloid beta 1-40	2.7	0.055	49	pH 7.4, 37 °C	PMID 25089527	Open record: neprilysin (neutral endopeptidase, NEP) variant G399V, amyloid beta 1-40
neprilysin (neutral endopeptidase, NEP)variant G399V	Homo sapiens	Angiotensin	0.73	0.0036	210	pH 7.4, 37 °C	PMID 25089527	Open record: neprilysin (neutral endopeptidase, NEP) variant G399V, Angiotensin
neprilysin (neutral endopeptidase, NEP)variant G399V	Homo sapiens	Bradykinin	0.15	0.079	1.9	pH 7.4, 37 °C	PMID 25089527	Open record: neprilysin (neutral endopeptidase, NEP) variant G399V, Bradykinin
neprilysin (neutral endopeptidase, NEP)variant G399V	Homo sapiens	Glucagon	2.9	0.02	150	pH 7.4, 37 °C	PMID 25089527	Open record: neprilysin (neutral endopeptidase, NEP) variant G399V, Glucagon
neprilysin (neutral endopeptidase, NEP)variant G399V	Homo sapiens	Neurotensin	1.1	0.012	100	pH 7.4, 37 °C	PMID 25089527	Open record: neprilysin (neutral endopeptidase, NEP) variant G399V, Neurotensin
neprilysin (neutral endopeptidase, NEP)variant G399V/G714K	Homo sapiens	amyloid beta 1-40	2.1	0.014	150	pH 7.4, 37 °C	PMID 25089527	Open record: neprilysin (neutral endopeptidase, NEP) variant G399V/G714K, amyloid beta 1-40
neprilysin (neutral endopeptidase, NEP)variant G399V/G714K	Homo sapiens	Angiotensin	0.14	0.013	11	pH 7.4, 37 °C	PMID 25089527	Open record: neprilysin (neutral endopeptidase, NEP) variant G399V/G714K, Angiotensin
neprilysin (neutral endopeptidase, NEP)variant G399V/G714K	Homo sapiens	Arg-vasopressin	0.017	0.022	0.9	pH 7.4, 37 °C	PMID 25089527	Open record: neprilysin (neutral endopeptidase, NEP) variant G399V/G714K, Arg-vasopressin
neprilysin (neutral endopeptidase, NEP)variant G399V/G714K	Homo sapiens	atrial natriuretic peptide	0.56	0.033	17	pH 7.4, 37 °C	PMID 25089527	Open record: neprilysin (neutral endopeptidase, NEP) variant G399V/G714K, atrial natriuretic peptide
neprilysin (neutral endopeptidase, NEP)variant G399V/G714K	Homo sapiens	Bradykinin	0.0055	0.037	0.15	pH 7.4, 37 °C	PMID 25089527	Open record: neprilysin (neutral endopeptidase, NEP) variant G399V/G714K, Bradykinin
neprilysin (neutral endopeptidase, NEP)variant G399V/G714K	Homo sapiens	Endothelin-1	0.059	0.057	1	37 °C	PMID 25089527	Open record: neprilysin (neutral endopeptidase, NEP) variant G399V/G714K, Endothelin-1
neprilysin (neutral endopeptidase, NEP)variant G399V/G714K	Homo sapiens	gastrin-releasing peptide	0.39	0.12	3.3	pH 7.4, 37 °C	PMID 25089527	Open record: neprilysin (neutral endopeptidase, NEP) variant G399V/G714K, gastrin-releasing peptide
neprilysin (neutral endopeptidase, NEP)variant G399V/G714K	Homo sapiens	Glucagon	1.2	0.03	46	pH 7.4, 37 °C	PMID 25089527	Open record: neprilysin (neutral endopeptidase, NEP) variant G399V/G714K, Glucagon
neprilysin (neutral endopeptidase, NEP)variant G399V/G714K	Homo sapiens	glucagon-like peptide-1	0.89	0.023	40	pH 7.4, 37 °C	PMID 25089527	Open record: neprilysin (neutral endopeptidase, NEP) variant G399V/G714K, glucagon-like peptide-1
neprilysin (neutral endopeptidase, NEP)variant G399V/G714K	Homo sapiens	Neurokinin A	1.1	0.034	34	pH 7.4, 37 °C	PMID 25089527	Open record: neprilysin (neutral endopeptidase, NEP) variant G399V/G714K, Neurokinin A
neprilysin (neutral endopeptidase, NEP)variant G399V/G714K	Homo sapiens	Neurokinin B	0.42	0.022	19	pH 7.4, 37 °C	PMID 25089527	Open record: neprilysin (neutral endopeptidase, NEP) variant G399V/G714K, Neurokinin B
neprilysin (neutral endopeptidase, NEP)variant G399V/G714K	Homo sapiens	Neurotensin	0.039	0.016	2.6	pH 7.4, 37 °C	PMID 25089527	Open record: neprilysin (neutral endopeptidase, NEP) variant G399V/G714K, Neurotensin
neprilysin (neutral endopeptidase, NEP)variant G399V/G714K	Homo sapiens	Nociceptin	14	0.063	220	pH 7.4, 37 °C	PMID 25089527	Open record: neprilysin (neutral endopeptidase, NEP) variant G399V/G714K, Nociceptin
neprilysin (neutral endopeptidase, NEP)variant G399V/G714K	Homo sapiens	Somatostatin 14	9.1	0.032	300	pH 7.4, 37 °C	PMID 25089527	Open record: neprilysin (neutral endopeptidase, NEP) variant G399V/G714K, Somatostatin 14
neprilysin (neutral endopeptidase, NEP)variant G399V/G714K	Homo sapiens	Somatostatin 28	0.086	0.047	1.8	pH 7.4, 37 °C	PMID 25089527	Open record: neprilysin (neutral endopeptidase, NEP) variant G399V/G714K, Somatostatin 28
neprilysin (neutral endopeptidase, NEP)variant G399V/G714K	Homo sapiens	Substance P	12	0.092	130	pH 7.4, 37 °C	PMID 25089527	Open record: neprilysin (neutral endopeptidase, NEP) variant G399V/G714K, Substance P
neprilysin (neutral endopeptidase, NEP)variant G399V/G714K	Homo sapiens	α-endorphin	0.089	0.0065	15	pH 7.4, 37 °C	PMID 25089527	Open record: neprilysin (neutral endopeptidase, NEP) variant G399V/G714K, α-endorphin
neprilysin (neutral endopeptidase, NEP)variant G399V/G714K	Homo sapiens	γ-endorphin	0.16	0.0044	38	pH 7.4, 37 °C	PMID 25089527	Open record: neprilysin (neutral endopeptidase, NEP) variant G399V/G714K, γ-endorphin
neprilysin (neutral endopeptidase, NEP)variant G714K	Homo sapiens	amyloid beta 1-40	1.9	0.042	45	pH 7.4, 37 °C	PMID 25089527	Open record: neprilysin (neutral endopeptidase, NEP) variant G714K, amyloid beta 1-40
neprilysin (neutral endopeptidase, NEP)variant G714K	Homo sapiens	Angiotensin	12	0.018	670	pH 7.4, 37 °C	PMID 25089527	Open record: neprilysin (neutral endopeptidase, NEP) variant G714K, Angiotensin
neprilysin (neutral endopeptidase, NEP)variant G714K	Homo sapiens	Bradykinin	7.4	0.12	64	pH 7.4, 37 °C	PMID 25089527	Open record: neprilysin (neutral endopeptidase, NEP) variant G714K, Bradykinin
neprilysin (neutral endopeptidase, NEP)variant G714K	Homo sapiens	Glucagon	5.2	0.042	120	pH 7.4, 37 °C	PMID 25089527	Open record: neprilysin (neutral endopeptidase, NEP) variant G714K, Glucagon
neprilysin (neutral endopeptidase, NEP)variant G714K	Homo sapiens	Neurotensin	8.6	0.01	840	pH 7.4, 37 °C	PMID 25089527	Open record: neprilysin (neutral endopeptidase, NEP) variant G714K, Neurotensin
neprilysin 2 (NEP2), secreted isoform NEP2(s)	Rattus norvegicus	3-methoxysuccinyl-Gly-Leu-Phe-7-amido-4-methylcoumarin	11.5	0.035	328	37 °C	PMID 11964170	Open record: neprilysin 2 (NEP2), secreted isoform NEP2(s), 3-methoxysuccinyl-Gly-Leu-Phe-7-amido-4-methylcoumarin
neprilysin 2 (NEP2), secreted isoform NEP2(s)	Rattus norvegicus	Arg-Pro-Lys-Pro-Gln-Gln-Phe-Phe-Gly-Leu-Met-NH2	6	—	—	pH 7.2, 37 °C	PMID 11964170	Open record: neprilysin 2 (NEP2), secreted isoform NEP2(s), Arg-Pro-Lys-Pro-Gln-Gln-Phe-Phe-Gly-Leu-Met-NH2
neprilysin 2 (NEP2), secreted isoform NEP2(s)	Rattus norvegicus	Arg-Pro-Lys-Pro-Gln-Gln-Phe-Phe-Gly-Leu-Met-NH2	—	0.013	—	pH 7.2, 37 °C	PMID 11964170	Open record: neprilysin 2 (NEP2), secreted isoform NEP2(s), Arg-Pro-Lys-Pro-Gln-Gln-Phe-Phe-Gly-Leu-Met-NH2
neprilysin 2 (NEP2), secreted isoform NEP2(s)	Rattus norvegicus	Arg-Pro-Pro-Gly-Phe-Ser-Pro-Phe-Arg	2.5	—	—	pH 7.2, 37 °C	PMID 11964170	Open record: neprilysin 2 (NEP2), secreted isoform NEP2(s), Arg-Pro-Pro-Gly-Phe-Ser-Pro-Phe-Arg
neprilysin 2 (NEP2), secreted isoform NEP2(s)	Rattus norvegicus	Asp-Arg-Val-Tyr-Ile-His-Pro-Phe-His-Leu	3	—	—	pH 7.2, 37 °C	PMID 11964170	Open record: neprilysin 2 (NEP2), secreted isoform NEP2(s), Asp-Arg-Val-Tyr-Ile-His-Pro-Phe-His-Leu
neprilysin 2 (NEP2), secreted isoform NEP2(s)	Rattus norvegicus	Asp-Tyr-Met-Gly-Trp-Met	34.5	—	—	pH 7.2, 37 °C	PMID 11964170	Open record: neprilysin 2 (NEP2), secreted isoform NEP2(s), Asp-Tyr-Met-Gly-Trp-Met
neprilysin 2 (NEP2), secreted isoform NEP2(s)	Rattus norvegicus	Asp-Tyr-Met-Gly-Trp-Met-Asp-Phe-NH2	13.2	—	—	pH 7.2, 37 °C	PMID 11964170	Open record: neprilysin 2 (NEP2), secreted isoform NEP2(s), Asp-Tyr-Met-Gly-Trp-Met-Asp-Phe-NH2
neprilysin 2 (NEP2), secreted isoform NEP2(s)	Rattus norvegicus	CCK-8 (sulphated)	6.2	—	—	pH 7.2, 37 °C	PMID 11964170	Open record: neprilysin 2 (NEP2), secreted isoform NEP2(s), CCK-8 (sulphated)
neprilysin 2 (NEP2), secreted isoform NEP2(s)	Rattus norvegicus	Gly-Gly-Phe-Met	8.5	—	—	pH 7.2, 37 °C	PMID 11964170	Open record: neprilysin 2 (NEP2), secreted isoform NEP2(s), Gly-Gly-Phe-Met
neprilysin 2 (NEP2), secreted isoform NEP2(s)	Rattus norvegicus	Gly-Trp-Met-Asp-Phe-NH2	4	—	—	pH 7.2, 37 °C	PMID 11964170	Open record: neprilysin 2 (NEP2), secreted isoform NEP2(s), Gly-Trp-Met-Asp-Phe-NH2
neprilysin 2 (NEP2), secreted isoform NEP2(s)	Rattus norvegicus	pGlu-His-Trp-Ser-Tyr-Gly-Leu-Arg-Pro-Gly-NH2	8.5	—	—	pH 7.2, 37 °C	PMID 11964170	Open record: neprilysin 2 (NEP2), secreted isoform NEP2(s), pGlu-His-Trp-Ser-Tyr-Gly-Leu-Arg-Pro-Gly-NH2
neprilysin 2 (NEP2), secreted isoform NEP2(s)	Rattus norvegicus	pGlu-His-Trp-Ser-Tyr-Gly-Leu-Arg-Pro-Gly-NH2	—	0.032	—	pH 7.2, 37 °C	PMID 11964170	Open record: neprilysin 2 (NEP2), secreted isoform NEP2(s), pGlu-His-Trp-Ser-Tyr-Gly-Leu-Arg-Pro-Gly-NH2
neprilysin 2 (NEP2), secreted isoform NEP2(s)	Rattus norvegicus	Phe-Gly-Leu-Met-NH2	22.8	—	—	pH 7.2, 37 °C	PMID 11964170	Open record: neprilysin 2 (NEP2), secreted isoform NEP2(s), Phe-Gly-Leu-Met-NH2
neprilysin 2 (NEP2), secreted isoform NEP2(s)	Rattus norvegicus	Phe-Phe-Gly-Leu-Met-NH2	50	—	—	pH 7.2, 37 °C	PMID 11964170	Open record: neprilysin 2 (NEP2), secreted isoform NEP2(s), Phe-Phe-Gly-Leu-Met-NH2
neprilysin 2 (NEP2), secreted isoform NEP2(s)	Rattus norvegicus	Phe-Phe-Gly-Leu-Met-NH2	—	0.081	—	pH 7.2, 37 °C	PMID 11964170	Open record: neprilysin 2 (NEP2), secreted isoform NEP2(s), Phe-Phe-Gly-Leu-Met-NH2
neprilysin 2 (NEP2), secreted isoform NEP2(s)	Rattus norvegicus	rANP-(1-28)	2	—	—	pH 7.2, 37 °C	PMID 11964170	Open record: neprilysin 2 (NEP2), secreted isoform NEP2(s), rANP-(1-28)
neprilysin 2 (NEP2), secreted isoform NEP2(s)	Rattus norvegicus	succinyl-Ala-Ala-Phe-7-amido-4-methylcoumarin	8.5	0.05	170	37 °C	PMID 11964170	Open record: neprilysin 2 (NEP2), secreted isoform NEP2(s), succinyl-Ala-Ala-Phe-7-amido-4-methylcoumarin
neprilysin 2 (NEP2), secreted isoform NEP2(s)	Rattus norvegicus	succinyl-Ala-Ala-Val-7-amido-4-methylcoumarin	13.8	0.1	138	37 °C	PMID 11964170	Open record: neprilysin 2 (NEP2), secreted isoform NEP2(s), succinyl-Ala-Ala-Val-7-amido-4-methylcoumarin
neprilysin 2 (NEP2), secreted isoform NEP2(s)	Rattus norvegicus	Tyr-Gly-Ala-Phe-Met	42	—	—	pH 7.2, 37 °C	PMID 11964170	Open record: neprilysin 2 (NEP2), secreted isoform NEP2(s), Tyr-Gly-Ala-Phe-Met
neprilysin 2 (NEP2), secreted isoform NEP2(s)	Rattus norvegicus	Tyr-Gly-Gly-Phe-Leu	2.7	—	—	pH 7.2, 37 °C	PMID 11964170	Open record: neprilysin 2 (NEP2), secreted isoform NEP2(s), Tyr-Gly-Gly-Phe-Leu
neprilysin 2 (NEP2), secreted isoform NEP2(s)	Rattus norvegicus	Tyr-Gly-Gly-Phe-Leu-NH2	0.33	—	—	pH 7.2, 37 °C	PMID 11964170	Open record: neprilysin 2 (NEP2), secreted isoform NEP2(s), Tyr-Gly-Gly-Phe-Leu-NH2
neprilysin 2 (NEP2), secreted isoform NEP2(s)	Rattus norvegicus	Tyr-Gly-Gly-Phe-Met	9	—	—	pH 7.2, 37 °C	PMID 11964170	Open record: neprilysin 2 (NEP2), secreted isoform NEP2(s), Tyr-Gly-Gly-Phe-Met
neprilysin 2 (NEP2), secreted isoform NEP2(s)	Rattus norvegicus	Tyr-Gly-Gly-Phe-Met	—	0.019	—	pH 7.2, 37 °C	PMID 11964170	Open record: neprilysin 2 (NEP2), secreted isoform NEP2(s), Tyr-Gly-Gly-Phe-Met
neprilysin 2 (NEP2), secreted isoform NEP2(s)	Rattus norvegicus	Tyr-Gly-Gly-Phe-Met-NH2	0.33	—	—	pH 7.2, 37 °C	PMID 11964170	Open record: neprilysin 2 (NEP2), secreted isoform NEP2(s), Tyr-Gly-Gly-Phe-Met-NH2
neprilysin 2 secreted isoform (rNEP2(s))	Rattus norvegicus	Suc-AAF-AMC	14.03	0.062	220	pH 7.2, 37 °C	PMID 15294904	Open record: neprilysin 2 secreted isoform (rNEP2(s)), Suc-AAF-AMC
neprilysin 2 secreted isoform (rNEP2(s))variant L739G	Rattus norvegicus	Suc-AAF-AMC	30.67	0.04	770	pH 7.2, 37 °C	PMID 15294904	Open record: neprilysin 2 secreted isoform (rNEP2(s)) variant L739G, Suc-AAF-AMC
neprilysin 2 secreted isoform (rNEP2(s))variant N567G	Rattus norvegicus	Suc-AAF-AMC	5.23	1	5	pH 7.2, 37 °C	PMID 15294904	Open record: neprilysin 2 secreted isoform (rNEP2(s)) variant N567G, Suc-AAF-AMC
neprilysin 2 secreted isoform (rNEP2(s))variant R131M	Rattus norvegicus	Suc-AAF-AMC	17.3	0.045	380	pH 7.2, 37 °C	PMID 15294904	Open record: neprilysin 2 secreted isoform (rNEP2(s)) variant R131M, Suc-AAF-AMC
neprilysin 2 secreted isoform (rNEP2(s))variant S133G	Rattus norvegicus	Suc-AAF-AMC	1.97	0.185	10	pH 7.2, 37 °C	PMID 15294904	Open record: neprilysin 2 secreted isoform (rNEP2(s)) variant S133G, Suc-AAF-AMC
neprilysin 2 secreted isoform (rNEP2(s))variant S133G/L739G	Rattus norvegicus	Suc-AAF-AMC	19	0.044	430	pH 7.2, 37 °C	PMID 15294904	Open record: neprilysin 2 secreted isoform (rNEP2(s)) variant S133G/L739G, Suc-AAF-AMC
neutral endopeptidase (NEP, neprilysin)	Lactococcus lactis subsp. cremoris	adrenocorticotropic hormone	0.028	0.0028	10.12	pH 7, 37 °C	PMID 8806762	Open record: neutral endopeptidase (NEP, neprilysin), adrenocorticotropic hormone
neutral endopeptidase (NEP, neprilysin)	Lactococcus lactis subsp. cremoris	alpha-neo-endorphin (Tyr-Gly-Gly-Phe-Leu-Arg-Lys-Tyr-Pro-Lys)	0.743	0.135	5.52	pH 7, 37 °C	PMID 8806762	Open record: neutral endopeptidase (NEP, neprilysin), alpha-neo-endorphin (Tyr-Gly-Gly-Phe-Leu-Arg-Lys-Tyr-Pro-Lys)
neutral endopeptidase (NEP, neprilysin)	Lactococcus lactis subsp. cremoris	angiotensin I (Asp-Arg-Val-Tyr-Ile-His-Pro-Phe-His-Leu)	—	4 × 10⁻⁴	—	pH 7, 37 °C	PMID 8806762	Open record: neutral endopeptidase (NEP, neprilysin), angiotensin I (Asp-Arg-Val-Tyr-Ile-His-Pro-Phe-His-Leu)
neutral endopeptidase (NEP, neprilysin)	Lactococcus lactis subsp. cremoris	beta-endorphin	0.43	0.0065	67	pH 7, 37 °C	PMID 8806762	Open record: neutral endopeptidase (NEP, neprilysin), beta-endorphin
neutral endopeptidase (NEP, neprilysin)	Lactococcus lactis subsp. cremoris	bradykinin (Arg-Pro-Pro-Gly-Phe-Ser-Pro-Phe-Arg)	0.072	0.005	14.27	pH 7, 37 °C	PMID 8806762	Open record: neutral endopeptidase (NEP, neprilysin), bradykinin (Arg-Pro-Pro-Gly-Phe-Ser-Pro-Phe-Arg)
neutral endopeptidase (NEP, neprilysin)	Lactococcus lactis subsp. cremoris	dynorphin A-10 (Tyr-Gly-Gly-Phe-Leu-Arg-Arg-Ile-Arg-Pro)	0.397	0.035	11.3	pH 7, 37 °C	PMID 8806762	Open record: neutral endopeptidase (NEP, neprilysin), dynorphin A-10 (Tyr-Gly-Gly-Phe-Leu-Arg-Arg-Ile-Arg-Pro)
neutral endopeptidase (NEP, neprilysin)	Lactococcus lactis subsp. cremoris	dynorphin A-13 (Tyr-Gly-Gly-Phe-Leu-Arg-Arg-Ile-Arg-Pro-Lys-Leu-Lys)	0.31	0.01	31	pH 7, 37 °C	PMID 8806762	Open record: neutral endopeptidase (NEP, neprilysin), dynorphin A-13 (Tyr-Gly-Gly-Phe-Leu-Arg-Arg-Ile-Arg-Pro-Lys-Leu-Lys)
neutral endopeptidase (NEP, neprilysin)	Lactococcus lactis subsp. cremoris	dynorphin A-17 (Tyr-Gly-Gly-Phe-Leu-Arg-Arg-Ile-Arg-Pro-Lys-Leu-Lys-Trp-Asp-Asn-Gln)	0.423	0.0082	51.57	pH 7, 37 °C	PMID 8806762	Open record: neutral endopeptidase (NEP, neprilysin), dynorphin A-17 (Tyr-Gly-Gly-Phe-Leu-Arg-Arg-Ile-Arg-Pro-Lys-Leu-Lys-Trp-Asp-Asn-Gln)
neutral endopeptidase (NEP, neprilysin)	Lactococcus lactis subsp. cremoris	dynorphin A-8 (Tyr-Gly-Gly-Phe-Leu-Arg-Arg-Ile)	0.327	0.298	1.1	pH 7, 37 °C	PMID 8806762	Open record: neutral endopeptidase (NEP, neprilysin), dynorphin A-8 (Tyr-Gly-Gly-Phe-Leu-Arg-Arg-Ile)
neutral endopeptidase (NEP, neprilysin)	Lactococcus lactis subsp. cremoris	dynorphin A-9 (Tyr-Gly-Gly-Phe-Leu-Arg-Arg-Ile-Arg)	0.537	0.04	13.42	pH 7, 37 °C	PMID 8806762	Open record: neutral endopeptidase (NEP, neprilysin), dynorphin A-9 (Tyr-Gly-Gly-Phe-Leu-Arg-Arg-Ile-Arg)
neutral endopeptidase (NEP, neprilysin)	Lactococcus lactis subsp. cremoris	glutaryl (or succinyl)-Ala-Ala-Phe-4-methoxy-2-naphthylamide (or 7-amino-4-methylcoumarin)	0.158	0.833	0.190 calc.	pH 7, 37 °C	PMID 8806762	Open record: neutral endopeptidase (NEP, neprilysin), glutaryl (or succinyl)-Ala-Ala-Phe-4-methoxy-2-naphthylamide (or 7-amino-4-methylcoumarin)
neutral endopeptidase (NEP, neprilysin)	Lactococcus lactis subsp. cremoris	Leu-enkephalin (Tyr-Gly-Gly-Phe-Leu)	0.25	0.2	1.25	pH 7, 37 °C	PMID 8806762	Open record: neutral endopeptidase (NEP, neprilysin), Leu-enkephalin (Tyr-Gly-Gly-Phe-Leu)
neutral endopeptidase (NEP, neprilysin)	Lactococcus lactis subsp. cremoris	Leu-enkephalin amide (Tyr-Gly-Gly-Phe-Leu-NH2)	0.137	0.612	0.22	pH 7, 37 °C	PMID 8806762	Open record: neutral endopeptidase (NEP, neprilysin), Leu-enkephalin amide (Tyr-Gly-Gly-Phe-Leu-NH2)
neutral endopeptidase (NEP, neprilysin)	Lactococcus lactis subsp. cremoris	Leu-enkephalin-Arg6, dynorphin A-6 (Tyr-Gly-Gly-Phe-Leu-Arg)	0.233	0.025	9.3	pH 7, 37 °C	PMID 8806762	Open record: neutral endopeptidase (NEP, neprilysin), Leu-enkephalin-Arg6, dynorphin A-6 (Tyr-Gly-Gly-Phe-Leu-Arg)
neutral endopeptidase (NEP, neprilysin)	Lactococcus lactis subsp. cremoris	Met-enkephalin (Tyr-Gly-Gly-Phe-Met)	0.13	0.1	1.3	pH 7, 37 °C	PMID 8806762	Open record: neutral endopeptidase (NEP, neprilysin), Met-enkephalin (Tyr-Gly-Gly-Phe-Met)
neutral endopeptidase (NEP, neprilysin)	Lactococcus lactis subsp. cremoris	Met-enkephalin amide (Tyr-Gly-Gly-Phe-Met-NH2)	0.0083	0.41	0.017	pH 7, 37 °C	PMID 8806762	Open record: neutral endopeptidase (NEP, neprilysin), Met-enkephalin amide (Tyr-Gly-Gly-Phe-Met-NH2)
neutral endopeptidase (NEP, neprilysin)	Lactococcus lactis subsp. cremoris	substance P (Arg-Pro-Lys-Pro-Gln-Gln-Phe-Phe-Gly-Leu-Met-NH2)	0.467	0.01	47	pH 7, 37 °C	PMID 8806762	Open record: neutral endopeptidase (NEP, neprilysin), substance P (Arg-Pro-Lys-Pro-Gln-Gln-Phe-Phe-Gly-Leu-Met-NH2)
neutral endopeptidase (NEP, neprilysin)	Lactococcus lactis subsp. cremoris	succinyl-Arg-Pro-Phe-His-Leu-Leu-Val-Tyr-7-amino-4-methylcoumarin	0.23	0.0062	38 calc.	pH 7, 37 °C	PMID 8806762	Open record: neutral endopeptidase (NEP, neprilysin), succinyl-Arg-Pro-Phe-His-Leu-Leu-Val-Tyr-7-amino-4-methylcoumarin
neutral endopeptidase (NEP, neprilysin)	Lactococcus lactis subsp. cremoris	vasoactive intestinal peptide	0.03	0.0018	17	pH 7, 37 °C	PMID 8806762	Open record: neutral endopeptidase (NEP, neprilysin), vasoactive intestinal peptide