CatLogenzyme kinetics catalog

Values CatLog read from this paper

Heterologous expression and characterization of recombinant Lactococcus lactis neutral endopeptidase (neprilysin)

PMID 8806762doi:10.1006/abbi.1996.0372Open in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 19 records (53 values) from this paper: neutral endopeptidase (NEP, neprilysin) from Lactococcus lactis subsp. cremoris.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 2 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.

EC 3.4.24.11

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)Conditions
neutral endopeptidase (NEP, neprilysin)Lactococcus lactis subsp. cremorisadrenocorticotropic hormone0.0280.002810.12pH 7, 37 °COpen
neutral endopeptidase (NEP, neprilysin)Lactococcus lactis subsp. cremorisalpha-neo-endorphin (Tyr-Gly-Gly-Phe-Leu-Arg-Lys-Tyr-Pro-Lys)0.7430.1355.52pH 7, 37 °COpen
neutral endopeptidase (NEP, neprilysin)Lactococcus lactis subsp. cremorisangiotensin I (Asp-Arg-Val-Tyr-Ile-His-Pro-Phe-His-Leu)—4 × 10⁻⁴—pH 7, 37 °COpen
neutral endopeptidase (NEP, neprilysin)Lactococcus lactis subsp. cremorisbeta-endorphin0.430.006567pH 7, 37 °COpen
neutral endopeptidase (NEP, neprilysin)Lactococcus lactis subsp. cremorisbradykinin (Arg-Pro-Pro-Gly-Phe-Ser-Pro-Phe-Arg)0.0720.00514.27pH 7, 37 °COpen
neutral endopeptidase (NEP, neprilysin)Lactococcus lactis subsp. cremorisdynorphin A-10 (Tyr-Gly-Gly-Phe-Leu-Arg-Arg-Ile-Arg-Pro)0.3970.03511.3pH 7, 37 °COpen
neutral endopeptidase (NEP, neprilysin)Lactococcus lactis subsp. cremorisdynorphin A-13 (Tyr-Gly-Gly-Phe-Leu-Arg-Arg-Ile-Arg-Pro-Lys-Leu-Lys)0.310.0131pH 7, 37 °COpen
neutral endopeptidase (NEP, neprilysin)Lactococcus lactis subsp. cremorisdynorphin A-17 (Tyr-Gly-Gly-Phe-Leu-Arg-Arg-Ile-Arg-Pro-Lys-Leu-Lys-Trp-Asp-Asn-Gln)0.4230.008251.57pH 7, 37 °COpen
neutral endopeptidase (NEP, neprilysin)Lactococcus lactis subsp. cremorisdynorphin A-8 (Tyr-Gly-Gly-Phe-Leu-Arg-Arg-Ile)0.3270.2981.1pH 7, 37 °COpen
neutral endopeptidase (NEP, neprilysin)Lactococcus lactis subsp. cremorisdynorphin A-9 (Tyr-Gly-Gly-Phe-Leu-Arg-Arg-Ile-Arg)0.5370.0413.42pH 7, 37 °COpen
neutral endopeptidase (NEP, neprilysin)Lactococcus lactis subsp. cremorisglutaryl (or succinyl)-Ala-Ala-Phe-4-methoxy-2-naphthylamide (or 7-amino-4-methylcoumarin)0.1580.8330.190 calc.pH 7, 37 °COpen
neutral endopeptidase (NEP, neprilysin)Lactococcus lactis subsp. cremorisLeu-enkephalin (Tyr-Gly-Gly-Phe-Leu)0.250.21.25pH 7, 37 °COpen
neutral endopeptidase (NEP, neprilysin)Lactococcus lactis subsp. cremorisLeu-enkephalin amide (Tyr-Gly-Gly-Phe-Leu-NH2)0.1370.6120.22pH 7, 37 °COpen
neutral endopeptidase (NEP, neprilysin)Lactococcus lactis subsp. cremorisLeu-enkephalin-Arg6, dynorphin A-6 (Tyr-Gly-Gly-Phe-Leu-Arg)0.2330.0259.3pH 7, 37 °COpen
neutral endopeptidase (NEP, neprilysin)Lactococcus lactis subsp. cremorisMet-enkephalin (Tyr-Gly-Gly-Phe-Met)0.130.11.3pH 7, 37 °COpen
neutral endopeptidase (NEP, neprilysin)Lactococcus lactis subsp. cremorisMet-enkephalin amide (Tyr-Gly-Gly-Phe-Met-NH2)0.00830.410.017pH 7, 37 °COpen
neutral endopeptidase (NEP, neprilysin)Lactococcus lactis subsp. cremorissubstance P (Arg-Pro-Lys-Pro-Gln-Gln-Phe-Phe-Gly-Leu-Met-NH2)0.4670.0147pH 7, 37 °COpen
neutral endopeptidase (NEP, neprilysin)Lactococcus lactis subsp. cremorissuccinyl-Arg-Pro-Phe-His-Leu-Leu-Val-Tyr-7-amino-4-methylcoumarin0.230.006238 calc.pH 7, 37 °COpen
neutral endopeptidase (NEP, neprilysin)Lactococcus lactis subsp. cremorisvasoactive intestinal peptide0.030.001817pH 7, 37 °COpen