Values CatLog read from this paper
Selective neurotensin-derived internally quenched fluorogenic substrates for neurolysin (EC 3.4.24.16): comparison with thimet oligopeptidase (EC 3.4.24.15) and neprilysin (EC 3.4.24.11)
PMID 11355859doi:10.1006/abio.2001.5083Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 36 records (82 values) from this paper: neurolysin, thimet oligopeptidase (TOP), neprilysin (NEP) and 1 more from Sus scrofa, Rattus norvegicus.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
EC 3.4.21.26, EC 3.4.24.11, EC 3.4.24.15, EC 3.4.24.16
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| neprilysin (NEP) | — | ortho-aminobenzoyl-Glu-Asn-Lys-Pro-Arg-Arg-Pro-Tyr-Gln-N-(2,4-dinitrophenyl)ethylenediamine | 3 | — | — | pH 8, 37 °C | Open record: neprilysin (NEP), ortho-aminobenzoyl-Glu-Asn-Lys-Pro-Arg-Arg-Pro-Tyr-Gln-N-(2,4-dinitrophenyl)ethylenediamine |
| neprilysin (NEP) | — | ortho-aminobenzoyl-Glu-Asn-Lys-Pro-Arg-Arg-Pro-Tyr-Ile-Gln-N-(2,4-dinitrophenyl)ethylenediamine | 1.4 | — | — | pH 8, 37 °C | Open record: neprilysin (NEP), ortho-aminobenzoyl-Glu-Asn-Lys-Pro-Arg-Arg-Pro-Tyr-Ile-Gln-N-(2,4-dinitrophenyl)ethylenediamine |
| neprilysin (NEP) | — | ortho-aminobenzoyl-Glu-Asn-Lys-Pro-Arg-Arg-Pro-Tyr-Ile-Leu-Gln-N-(2,4-dinitrophenyl)ethylenediamine | 12 | — | — | pH 8, 37 °C | Open record: neprilysin (NEP), ortho-aminobenzoyl-Glu-Asn-Lys-Pro-Arg-Arg-Pro-Tyr-Ile-Leu-Gln-N-(2,4-dinitrophenyl)ethylenediamine |
| neprilysin (NEP) | — | ortho-aminobenzoyl-Leu-Tyr-Glu-Asn-Lys-Pro-Arg-Arg-Pro-Tyr-Ile-Leu-Gln-N-(2,4-dinitrophenyl)ethylenediamine | 0.8 | — | — | pH 8, 37 °C | Open record: neprilysin (NEP), ortho-aminobenzoyl-Leu-Tyr-Glu-Asn-Lys-Pro-Arg-Arg-Pro-Tyr-Ile-Leu-Gln-N-(2,4-dinitrophenyl)ethylenediamine |
| neprilysin (NEP) | — | ortho-aminobenzoyl-Lys-Pro-Arg-Arg-Pro-Tyr-Ile-Leu-Gln-N-(2,4-dinitrophenyl)ethylenediamine | 0.3 | — | — | pH 8, 37 °C | Open record: neprilysin (NEP), ortho-aminobenzoyl-Lys-Pro-Arg-Arg-Pro-Tyr-Ile-Leu-Gln-N-(2,4-dinitrophenyl)ethylenediamine |
| neurolysin | Sus scrofa | ortho-aminobenzoyl-Ala-Lys-Pro-Arg-Arg-Pro-Gln-N-(2,4-dinitrophenyl)ethylenediamine | 4.97 | 0.00115 | 4322 | pH 7.4, 37 °C | Open record: neurolysin, ortho-aminobenzoyl-Ala-Lys-Pro-Arg-Arg-Pro-Gln-N-(2,4-dinitrophenyl)ethylenediamine |
| neurolysin | Sus scrofa | ortho-aminobenzoyl-Asn-Ala-Pro-Arg-Arg-Pro-Gln-N-(2,4-dinitrophenyl)ethylenediamine | 6.97 | 0.00103 | 6767 | pH 7.4, 37 °C | Open record: neurolysin, ortho-aminobenzoyl-Asn-Ala-Pro-Arg-Arg-Pro-Gln-N-(2,4-dinitrophenyl)ethylenediamine |
| neurolysin | Sus scrofa | ortho-aminobenzoyl-Asn-Lys-Ala-Arg-Arg-Pro-Gln-N-(2,4-dinitrophenyl)ethylenediamine | 5.76 | 0.00138 | 4174 | pH 7.4, 37 °C | Open record: neurolysin, ortho-aminobenzoyl-Asn-Lys-Ala-Arg-Arg-Pro-Gln-N-(2,4-dinitrophenyl)ethylenediamine |
| neurolysin | Sus scrofa | ortho-aminobenzoyl-Asn-Lys-Pro-Ala-Arg-Pro-Gln-N-(2,4-dinitrophenyl)ethylenediamine | 1.23 | 5.6 × 10⁻⁴ | 2196 | pH 7.4, 37 °C | Open record: neurolysin, ortho-aminobenzoyl-Asn-Lys-Pro-Ala-Arg-Pro-Gln-N-(2,4-dinitrophenyl)ethylenediamine |
| neurolysin | Sus scrofa | ortho-aminobenzoyl-Asn-Lys-Pro-Arg-Ala-Pro-Gln-N-(2,4-dinitrophenyl)ethylenediamine | 14.03 | 8.2 × 10⁻⁴ | 1.711 × 10⁴ | pH 7.4, 37 °C | Open record: neurolysin, ortho-aminobenzoyl-Asn-Lys-Pro-Arg-Ala-Pro-Gln-N-(2,4-dinitrophenyl)ethylenediamine |
| neurolysin | Sus scrofa | ortho-aminobenzoyl-Asn-Lys-Pro-Arg-Arg-Ala-Gln-N-(2,4-dinitrophenyl)ethylenediamine | 1.24 | 2.5 × 10⁻⁴ | 4.96 × 10³ | pH 7.4, 37 °C | Open record: neurolysin, ortho-aminobenzoyl-Asn-Lys-Pro-Arg-Arg-Ala-Gln-N-(2,4-dinitrophenyl)ethylenediamine |
| neurolysin | Sus scrofa | ortho-aminobenzoyl-Asn-Lys-Pro-Arg-Arg-Pro-Gln-N-(2,4-dinitrophenyl)ethylenediamine | 7 | 0.00119 | 5882 | pH 7.4, 37 °C | Open record: neurolysin, ortho-aminobenzoyl-Asn-Lys-Pro-Arg-Arg-Pro-Gln-N-(2,4-dinitrophenyl)ethylenediamine |
| neurolysin | Sus scrofa | ortho-aminobenzoyl-Glu-Asn-Lys-Pro-Arg-Arg-Pro-Gln-N-(2,4-dinitrophenyl)ethylenediamine | 1.35 | 0.0058 | 233 | pH 7.4, 37 °C | Open record: neurolysin, ortho-aminobenzoyl-Glu-Asn-Lys-Pro-Arg-Arg-Pro-Gln-N-(2,4-dinitrophenyl)ethylenediamine |
| neurolysin | Sus scrofa | ortho-aminobenzoyl-Glu-Asn-Lys-Pro-Arg-Arg-Pro-Tyr-Gln-N-(2,4-dinitrophenyl)ethylenediamine | 0.8 | — | — | pH 7.4, 37 °C | Open record: neurolysin, ortho-aminobenzoyl-Glu-Asn-Lys-Pro-Arg-Arg-Pro-Tyr-Gln-N-(2,4-dinitrophenyl)ethylenediamine |
| neurolysin | Sus scrofa | ortho-aminobenzoyl-Glu-Asn-Lys-Pro-Arg-Arg-Pro-Tyr-Ile-Gln-N-(2,4-dinitrophenyl)ethylenediamine | 2.3 | — | — | pH 7.4, 37 °C | Open record: neurolysin, ortho-aminobenzoyl-Glu-Asn-Lys-Pro-Arg-Arg-Pro-Tyr-Ile-Gln-N-(2,4-dinitrophenyl)ethylenediamine |
| neurolysin | Sus scrofa | ortho-aminobenzoyl-Glu-Asn-Lys-Pro-Arg-Arg-Pro-Tyr-Ile-Leu-Gln-N-(2,4-dinitrophenyl)ethylenediamine | 1.6 | — | — | pH 7.4, 37 °C | Open record: neurolysin, ortho-aminobenzoyl-Glu-Asn-Lys-Pro-Arg-Arg-Pro-Tyr-Ile-Leu-Gln-N-(2,4-dinitrophenyl)ethylenediamine |
| neurolysin | Sus scrofa | ortho-aminobenzoyl-Gly-Gly-Phe-Leu-Pro-Arg-Arg-Pro-Gln-N-(2,4-dinitrophenyl)ethylenediamine | 1.77 | 4.7 × 10⁻⁴ | 3766 | pH 7.4, 37 °C | Open record: neurolysin, ortho-aminobenzoyl-Gly-Gly-Phe-Leu-Pro-Arg-Arg-Pro-Gln-N-(2,4-dinitrophenyl)ethylenediamine |
| neurolysin | Sus scrofa | ortho-aminobenzoyl-Leu-Tyr-Glu-Asn-Lys-Pro-Arg-Arg-Pro-Gln-N-(2,4-dinitrophenyl)ethylenediamine | 3.8 | 4.4 × 10⁻⁴ | 8636 | pH 7.4, 37 °C | Open record: neurolysin, ortho-aminobenzoyl-Leu-Tyr-Glu-Asn-Lys-Pro-Arg-Arg-Pro-Gln-N-(2,4-dinitrophenyl)ethylenediamine |
| neurolysin | Sus scrofa | ortho-aminobenzoyl-Leu-Tyr-Glu-Asn-Lys-Pro-Arg-Arg-Pro-Tyr-Ile-Leu-Gln-N-(2,4-dinitrophenyl)ethylenediamine | 1.5 | — | — | pH 7.4, 37 °C | Open record: neurolysin, ortho-aminobenzoyl-Leu-Tyr-Glu-Asn-Lys-Pro-Arg-Arg-Pro-Tyr-Ile-Leu-Gln-N-(2,4-dinitrophenyl)ethylenediamine |
| neurolysin | Sus scrofa | ortho-aminobenzoyl-Lys-Pro-Arg-Arg-Pro-Gln-N-(2,4-dinitrophenyl)ethylenediamine | 2.63 | 0.0033 | 797 | pH 7.4, 37 °C | Open record: neurolysin, ortho-aminobenzoyl-Lys-Pro-Arg-Arg-Pro-Gln-N-(2,4-dinitrophenyl)ethylenediamine |
| neurolysin | Sus scrofa | ortho-aminobenzoyl-Lys-Pro-Arg-Arg-Pro-Tyr-Ile-Leu-Gln-N-(2,4-dinitrophenyl)ethylenediamine | 0.4 | — | — | pH 7.4, 37 °C | Open record: neurolysin, ortho-aminobenzoyl-Lys-Pro-Arg-Arg-Pro-Tyr-Ile-Leu-Gln-N-(2,4-dinitrophenyl)ethylenediamine |
| prolyl oligopeptidase | Sus scrofa | ortho-aminobenzoyl-Asn-Lys-Pro-Arg-Arg-Pro-Gln-N-(2,4-dinitrophenyl)ethylenediamine | 0.03 | 4.4 × 10⁻⁴ | 68 | pH 7.5, 37 °C | Open record: prolyl oligopeptidase, ortho-aminobenzoyl-Asn-Lys-Pro-Arg-Arg-Pro-Gln-N-(2,4-dinitrophenyl)ethylenediamine |
| thimet oligopeptidase (TOP) | Rattus norvegicus | ortho-aminobenzoyl-Ala-Lys-Pro-Arg-Arg-Pro-Gln-N-(2,4-dinitrophenyl)ethylenediamine | 0.75 | 0.0102 | 74 | pH 7.4, 37 °C | Open record: thimet oligopeptidase (TOP), ortho-aminobenzoyl-Ala-Lys-Pro-Arg-Arg-Pro-Gln-N-(2,4-dinitrophenyl)ethylenediamine |
| thimet oligopeptidase (TOP) | Rattus norvegicus | ortho-aminobenzoyl-Asn-Ala-Pro-Arg-Arg-Pro-Gln-N-(2,4-dinitrophenyl)ethylenediamine | 3.6 | 0.0071 | 507 | pH 7.4, 37 °C | Open record: thimet oligopeptidase (TOP), ortho-aminobenzoyl-Asn-Ala-Pro-Arg-Arg-Pro-Gln-N-(2,4-dinitrophenyl)ethylenediamine |
| thimet oligopeptidase (TOP) | Rattus norvegicus | ortho-aminobenzoyl-Asn-Lys-Ala-Arg-Arg-Pro-Gln-N-(2,4-dinitrophenyl)ethylenediamine | 8 | 0.064 | 125 | pH 7.4, 37 °C | Open record: thimet oligopeptidase (TOP), ortho-aminobenzoyl-Asn-Lys-Ala-Arg-Arg-Pro-Gln-N-(2,4-dinitrophenyl)ethylenediamine |
| thimet oligopeptidase (TOP) | Rattus norvegicus | ortho-aminobenzoyl-Asn-Lys-Pro-Ala-Arg-Pro-Gln-N-(2,4-dinitrophenyl)ethylenediamine | 4.2 | 0.036 | 117 | pH 7.4, 37 °C | Open record: thimet oligopeptidase (TOP), ortho-aminobenzoyl-Asn-Lys-Pro-Ala-Arg-Pro-Gln-N-(2,4-dinitrophenyl)ethylenediamine |
| thimet oligopeptidase (TOP) | Rattus norvegicus | ortho-aminobenzoyl-Asn-Lys-Pro-Arg-Ala-Pro-Gln-N-(2,4-dinitrophenyl)ethylenediamine | 8.7 | 0.015 | 580 | pH 7.4, 37 °C | Open record: thimet oligopeptidase (TOP), ortho-aminobenzoyl-Asn-Lys-Pro-Arg-Ala-Pro-Gln-N-(2,4-dinitrophenyl)ethylenediamine |
| thimet oligopeptidase (TOP) | Rattus norvegicus | ortho-aminobenzoyl-Asn-Lys-Pro-Arg-Arg-Ala-Gln-N-(2,4-dinitrophenyl)ethylenediamine | 5.7 | 0.0124 | 460 | pH 7.4, 37 °C | Open record: thimet oligopeptidase (TOP), ortho-aminobenzoyl-Asn-Lys-Pro-Arg-Arg-Ala-Gln-N-(2,4-dinitrophenyl)ethylenediamine |
| thimet oligopeptidase (TOP) | Rattus norvegicus | ortho-aminobenzoyl-Asn-Lys-Pro-Arg-Arg-Pro-Gln-N-(2,4-dinitrophenyl)ethylenediamine | 0.7 | 0.0121 | 58 | pH 7.4, 37 °C | Open record: thimet oligopeptidase (TOP), ortho-aminobenzoyl-Asn-Lys-Pro-Arg-Arg-Pro-Gln-N-(2,4-dinitrophenyl)ethylenediamine |
| thimet oligopeptidase (TOP) | Rattus norvegicus | ortho-aminobenzoyl-Glu-Asn-Lys-Pro-Arg-Arg-Pro-Gln-N-(2,4-dinitrophenyl)ethylenediamine | 3.2 | 0.021 | 152 | pH 7.4, 37 °C | Open record: thimet oligopeptidase (TOP), ortho-aminobenzoyl-Glu-Asn-Lys-Pro-Arg-Arg-Pro-Gln-N-(2,4-dinitrophenyl)ethylenediamine |
| thimet oligopeptidase (TOP) | Rattus norvegicus | ortho-aminobenzoyl-Glu-Asn-Lys-Pro-Arg-Arg-Pro-Tyr-Gln-N-(2,4-dinitrophenyl)ethylenediamine | 1.1 | — | — | pH 7.4, 37 °C | Open record: thimet oligopeptidase (TOP), ortho-aminobenzoyl-Glu-Asn-Lys-Pro-Arg-Arg-Pro-Tyr-Gln-N-(2,4-dinitrophenyl)ethylenediamine |
| thimet oligopeptidase (TOP) | Rattus norvegicus | ortho-aminobenzoyl-Glu-Asn-Lys-Pro-Arg-Arg-Pro-Tyr-Ile-Gln-N-(2,4-dinitrophenyl)ethylenediamine | 0.9 | — | — | pH 7.4, 37 °C | Open record: thimet oligopeptidase (TOP), ortho-aminobenzoyl-Glu-Asn-Lys-Pro-Arg-Arg-Pro-Tyr-Ile-Gln-N-(2,4-dinitrophenyl)ethylenediamine |
| thimet oligopeptidase (TOP) | Rattus norvegicus | ortho-aminobenzoyl-Gly-Gly-Phe-Leu-Pro-Arg-Arg-Pro-Gln-N-(2,4-dinitrophenyl)ethylenediamine | 3.7 | 0.0028 | 1321 | pH 7.4, 37 °C | Open record: thimet oligopeptidase (TOP), ortho-aminobenzoyl-Gly-Gly-Phe-Leu-Pro-Arg-Arg-Pro-Gln-N-(2,4-dinitrophenyl)ethylenediamine |
| thimet oligopeptidase (TOP) | Rattus norvegicus | ortho-aminobenzoyl-Leu-Tyr-Glu-Asn-Lys-Pro-Arg-Arg-Pro-Gln-N-(2,4-dinitrophenyl)ethylenediamine | 0.3 | 0.007 | 43 | pH 7.4, 37 °C | Open record: thimet oligopeptidase (TOP), ortho-aminobenzoyl-Leu-Tyr-Glu-Asn-Lys-Pro-Arg-Arg-Pro-Gln-N-(2,4-dinitrophenyl)ethylenediamine |
| thimet oligopeptidase (TOP) | Rattus norvegicus | ortho-aminobenzoyl-Leu-Tyr-Glu-Asn-Lys-Pro-Arg-Arg-Pro-Tyr-Ile-Leu-Gln-N-(2,4-dinitrophenyl)ethylenediamine | 0.3 | — | — | pH 7.4, 37 °C | Open record: thimet oligopeptidase (TOP), ortho-aminobenzoyl-Leu-Tyr-Glu-Asn-Lys-Pro-Arg-Arg-Pro-Tyr-Ile-Leu-Gln-N-(2,4-dinitrophenyl)ethylenediamine |
| thimet oligopeptidase (TOP) | Rattus norvegicus | ortho-aminobenzoyl-Lys-Pro-Arg-Arg-Pro-Gln-N-(2,4-dinitrophenyl)ethylenediamine | 3.7 | 0.114 | 32 | pH 7.4, 37 °C | Open record: thimet oligopeptidase (TOP), ortho-aminobenzoyl-Lys-Pro-Arg-Arg-Pro-Gln-N-(2,4-dinitrophenyl)ethylenediamine |