Values CatLog read from this paper
Molecular basis for enantioselectivity in the (R)- and (S)-hydroxypropylthioethanesulfonate dehydrogenases, a unique pair of stereoselective short-chain dehydrogenases/reductases involved in…
PMID 20302306doi:10.1021/bi100294mOpen in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 29 records (79 values) from this paper: S-HPCDH3 ((S)-2-hydroxypropyl-CoM dehydrogenase 3, XecE3), R-HPCDH1 ((R)-2-hydroxypropyl-CoM dehydrogenase 1, XecD1), S-HPCDH1 ((S)-2-hydroxypropyl-CoM dehydrogenase 1, XecE1) from Xanthobacter autotrophicus.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| R-HPCDH1 ((R)-2-hydroxypropyl-CoM dehydrogenase 1, XecD1) | Xanthobacter autotrophicus | (R)-2-(2-hydroxypropylthio)ethanesulfonate | 48 | 0.096 | 500 | pH 7.5, 30 °C | Open record: R-HPCDH1 ((R)-2-hydroxypropyl-CoM dehydrogenase 1, XecD1), (R)-2-(2-hydroxypropylthio)ethanesulfonate |
| R-HPCDH1 ((R)-2-hydroxypropyl-CoM dehydrogenase 1, XecD1) | Xanthobacter autotrophicus | (R)-2-butanol | 1.9 | 220 | 0.00869 | pH 7.5, 30 °C | Open record: R-HPCDH1 ((R)-2-hydroxypropyl-CoM dehydrogenase 1, XecD1), (R)-2-butanol |
| R-HPCDH1 ((R)-2-hydroxypropyl-CoM dehydrogenase 1, XecD1) | Xanthobacter autotrophicus | (S)-2-(2-hydroxypropylthio)ethanesulfonate | 0.12 | 0.22 | 0.53 | pH 7.5, 30 °C | Open record: R-HPCDH1 ((R)-2-hydroxypropyl-CoM dehydrogenase 1, XecD1), (S)-2-(2-hydroxypropylthio)ethanesulfonate |
| R-HPCDH1 ((R)-2-hydroxypropyl-CoM dehydrogenase 1, XecD1) | Xanthobacter autotrophicus | (S)-2-butanol | 9.3 | 350 | 0.0264 | pH 7.5, 30 °C | Open record: R-HPCDH1 ((R)-2-hydroxypropyl-CoM dehydrogenase 1, XecD1), (S)-2-butanol |
| R-HPCDH1 ((R)-2-hydroxypropyl-CoM dehydrogenase 1, XecD1) | Xanthobacter autotrophicus | 2-(2-hydroxyethylthio)ethanesulfonate | — | 0.96 | — | pH 7.5, 30 °C | Open record: R-HPCDH1 ((R)-2-hydroxypropyl-CoM dehydrogenase 1, XecD1), 2-(2-hydroxyethylthio)ethanesulfonate |
| R-HPCDH1 ((R)-2-hydroxypropyl-CoM dehydrogenase 1, XecD1) | Xanthobacter autotrophicus | 2-(2-oxopropylthio)ethanesulfonate | 29 | 0.068 | 420 | pH 7.5, 30 °C | Open record: R-HPCDH1 ((R)-2-hydroxypropyl-CoM dehydrogenase 1, XecD1), 2-(2-oxopropylthio)ethanesulfonate |
| S-HPCDH1 ((S)-2-hydroxypropyl-CoM dehydrogenase 1, XecE1) | Xanthobacter autotrophicus | (R)-2-(2-hydroxypropylthio)ethanesulfonate | 2.4 | 5.2 | 0.46 | pH 7.5, 30 °C | Open record: S-HPCDH1 ((S)-2-hydroxypropyl-CoM dehydrogenase 1, XecE1), (R)-2-(2-hydroxypropylthio)ethanesulfonate |
| S-HPCDH1 ((S)-2-hydroxypropyl-CoM dehydrogenase 1, XecE1) | Xanthobacter autotrophicus | (S)-2-(2-hydroxypropylthio)ethanesulfonate | 28 | 0.22 | 130 | pH 7.5, 30 °C | Open record: S-HPCDH1 ((S)-2-hydroxypropyl-CoM dehydrogenase 1, XecE1), (S)-2-(2-hydroxypropylthio)ethanesulfonate |
| S-HPCDH1 ((S)-2-hydroxypropyl-CoM dehydrogenase 1, XecE1) | Xanthobacter autotrophicus | 2-(2-oxopropylthio)ethanesulfonate | 20 | 0.24 | 83 | pH 7.5, 30 °C | Open record: S-HPCDH1 ((S)-2-hydroxypropyl-CoM dehydrogenase 1, XecE1), 2-(2-oxopropylthio)ethanesulfonate |
| S-HPCDH3 ((S)-2-hydroxypropyl-CoM dehydrogenase 3, XecE3) | Xanthobacter autotrophicus | (R)-2-(2-hydroxypropylthio)ethanesulfonate | 5.5 | 9.1 | 1.2 | pH 7.5, 30 °C | Open record: S-HPCDH3 ((S)-2-hydroxypropyl-CoM dehydrogenase 3, XecE3), (R)-2-(2-hydroxypropylthio)ethanesulfonate |
| S-HPCDH3 ((S)-2-hydroxypropyl-CoM dehydrogenase 3, XecE3) | Xanthobacter autotrophicus | (R)-2-butanol | 1 | 68 | 0.0148 | pH 7.5, 30 °C | Open record: S-HPCDH3 ((S)-2-hydroxypropyl-CoM dehydrogenase 3, XecE3), (R)-2-butanol |
| S-HPCDH3 ((S)-2-hydroxypropyl-CoM dehydrogenase 3, XecE3) | Xanthobacter autotrophicus | (S)-2-(2-hydroxypropylthio)ethanesulfonate | 25 | 0.031 | 790 | pH 7.5, 30 °C | Open record: S-HPCDH3 ((S)-2-hydroxypropyl-CoM dehydrogenase 3, XecE3), (S)-2-(2-hydroxypropylthio)ethanesulfonate |
| S-HPCDH3 ((S)-2-hydroxypropyl-CoM dehydrogenase 3, XecE3) | Xanthobacter autotrophicus | (S)-2-butanol | 2.6 | 28 | 0.0928 | pH 7.5, 30 °C | Open record: S-HPCDH3 ((S)-2-hydroxypropyl-CoM dehydrogenase 3, XecE3), (S)-2-butanol |
| S-HPCDH3 ((S)-2-hydroxypropyl-CoM dehydrogenase 3, XecE3) | Xanthobacter autotrophicus | 2-(2-hydroxyethylthio)ethanesulfonate | — | 0.97 | — | pH 7.5, 30 °C | Open record: S-HPCDH3 ((S)-2-hydroxypropyl-CoM dehydrogenase 3, XecE3), 2-(2-hydroxyethylthio)ethanesulfonate |
| S-HPCDH3 ((S)-2-hydroxypropyl-CoM dehydrogenase 3, XecE3) | Xanthobacter autotrophicus | 2-(2-oxopropylthio)ethanesulfonate | 11 | 0.27 | 39 | pH 7.5, 30 °C | Open record: S-HPCDH3 ((S)-2-hydroxypropyl-CoM dehydrogenase 3, XecE3), 2-(2-oxopropylthio)ethanesulfonate |
| S-HPCDH3 ((S)-2-hydroxypropyl-CoM dehydrogenase 3, XecE3) | Xanthobacter autotrophicus | 2-butanone | 0.044 | 120 | 3.7 × 10⁻⁴ | pH 7.5, 30 °C | Open record: S-HPCDH3 ((S)-2-hydroxypropyl-CoM dehydrogenase 3, XecE3), 2-butanone |
| S-HPCDH3 ((S)-2-hydroxypropyl-CoM dehydrogenase 3, XecE3) | Xanthobacter autotrophicus | 2-propanol | 2 | 1.4 × 10³ | 0.0014 | pH 7.5, 30 °C | Open record: S-HPCDH3 ((S)-2-hydroxypropyl-CoM dehydrogenase 3, XecE3), 2-propanol |
| S-HPCDH3 ((S)-2-hydroxypropyl-CoM dehydrogenase 3, XecE3) | Xanthobacter autotrophicus | NAD+ | — | 0.191 | — | pH 7.5, 30 °C | Open record: S-HPCDH3 ((S)-2-hydroxypropyl-CoM dehydrogenase 3, XecE3), NAD+ |
| S-HPCDH3 ((S)-2-hydroxypropyl-CoM dehydrogenase 3, XecE3) | Xanthobacter autotrophicus | NADH | — | 0.00842 | — | pH 7.5, 30 °C | Open record: S-HPCDH3 ((S)-2-hydroxypropyl-CoM dehydrogenase 3, XecE3), NADH |
| S-HPCDH3 ((S)-2-hydroxypropyl-CoM dehydrogenase 3, XecE3)variant K214A | Xanthobacter autotrophicus | (S)-2-(2-hydroxypropylthio)ethanesulfonate | 5.8 | 2.3 | 2.5 | pH 7.5, 30 °C | Open record: S-HPCDH3 ((S)-2-hydroxypropyl-CoM dehydrogenase 3, XecE3) variant K214A, (S)-2-(2-hydroxypropylthio)ethanesulfonate |
| S-HPCDH3 ((S)-2-hydroxypropyl-CoM dehydrogenase 3, XecE3)variant K214A | Xanthobacter autotrophicus | 2-(2-oxopropylthio)ethanesulfonate | 9.6 | 12 | 0.81 | pH 7.5, 30 °C | Open record: S-HPCDH3 ((S)-2-hydroxypropyl-CoM dehydrogenase 3, XecE3) variant K214A, 2-(2-oxopropylthio)ethanesulfonate |
| S-HPCDH3 ((S)-2-hydroxypropyl-CoM dehydrogenase 3, XecE3)variant K214A | Xanthobacter autotrophicus | 2-butanone | 0.039 | 72 | 5.4 × 10⁻⁴ | pH 7.5, 30 °C | Open record: S-HPCDH3 ((S)-2-hydroxypropyl-CoM dehydrogenase 3, XecE3) variant K214A, 2-butanone |
| S-HPCDH3 ((S)-2-hydroxypropyl-CoM dehydrogenase 3, XecE3)variant K214A | Xanthobacter autotrophicus | 2-propanol | 1.7 | 950 | 0.0018 | pH 7.5, 30 °C | Open record: S-HPCDH3 ((S)-2-hydroxypropyl-CoM dehydrogenase 3, XecE3) variant K214A, 2-propanol |
| S-HPCDH3 ((S)-2-hydroxypropyl-CoM dehydrogenase 3, XecE3)variant R211A | Xanthobacter autotrophicus | (S)-2-(2-hydroxypropylthio)ethanesulfonate | 16 | 1.6 | 10 | pH 7.5, 30 °C | Open record: S-HPCDH3 ((S)-2-hydroxypropyl-CoM dehydrogenase 3, XecE3) variant R211A, (S)-2-(2-hydroxypropylthio)ethanesulfonate |
| S-HPCDH3 ((S)-2-hydroxypropyl-CoM dehydrogenase 3, XecE3)variant R211A | Xanthobacter autotrophicus | 2-(2-oxopropylthio)ethanesulfonate | 8.6 | 11 | 0.75 | pH 7.5, 30 °C | Open record: S-HPCDH3 ((S)-2-hydroxypropyl-CoM dehydrogenase 3, XecE3) variant R211A, 2-(2-oxopropylthio)ethanesulfonate |
| S-HPCDH3 ((S)-2-hydroxypropyl-CoM dehydrogenase 3, XecE3)variant R211A | Xanthobacter autotrophicus | 2-butanone | 0.072 | 180 | 4 × 10⁻⁴ | pH 7.5, 30 °C | Open record: S-HPCDH3 ((S)-2-hydroxypropyl-CoM dehydrogenase 3, XecE3) variant R211A, 2-butanone |
| S-HPCDH3 ((S)-2-hydroxypropyl-CoM dehydrogenase 3, XecE3)variant R211A | Xanthobacter autotrophicus | 2-propanol | 1.7 | 720 | 0.0024 | pH 7.5, 30 °C | Open record: S-HPCDH3 ((S)-2-hydroxypropyl-CoM dehydrogenase 3, XecE3) variant R211A, 2-propanol |
| S-HPCDH3 ((S)-2-hydroxypropyl-CoM dehydrogenase 3, XecE3)variant S143A | Xanthobacter autotrophicus | (S)-2-(2-hydroxypropylthio)ethanesulfonate | 0.013 | 9.5 | 0.0014 | pH 7.5, 30 °C | Open record: S-HPCDH3 ((S)-2-hydroxypropyl-CoM dehydrogenase 3, XecE3) variant S143A, (S)-2-(2-hydroxypropylthio)ethanesulfonate |
| S-HPCDH3 ((S)-2-hydroxypropyl-CoM dehydrogenase 3, XecE3)variant Y156A | Xanthobacter autotrophicus | (S)-2-(2-hydroxypropylthio)ethanesulfonate | 0.65 | 1.8 | 0.35 | pH 7.5, 30 °C | Open record: S-HPCDH3 ((S)-2-hydroxypropyl-CoM dehydrogenase 3, XecE3) variant Y156A, (S)-2-(2-hydroxypropylthio)ethanesulfonate |