Values CatLog read from this paper
The functions of key residues in the inhibitor, substrate and cofactor sites of human 3beta-hydroxysteroid dehydrogenase type 1 are validated by mutagenesis
PMID 20420909doi:10.1016/j.jsbmb.2010.04.015Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 23 records (67 values) from this paper: 3β-hydroxysteroid dehydrogenase/Δ5→4-isomerase type 1 (3β-HSD1), 3β-hydroxysteroid dehydrogenase/Δ5→4-isomerase type 2 (3β-HSD2) from Homo sapiens.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| 3β-hydroxysteroid dehydrogenase/Δ5→4-isomerase type 1 (3β-HSD1) | Homo sapiens | 16α-hydroxydehydroepiandrosterone | 0.097 | 0.0064 | 15 | pH 7.4, 27 °C | Open record: 3β-hydroxysteroid dehydrogenase/Δ5→4-isomerase type 1 (3β-HSD1), 16α-hydroxydehydroepiandrosterone |
| 3β-hydroxysteroid dehydrogenase/Δ5→4-isomerase type 1 (3β-HSD1) | Homo sapiens | 16β-hydroxydehydroepiandrosterone | 0.048 | 0.0014 | 34.5 | pH 7.4, 27 °C | Open record: 3β-hydroxysteroid dehydrogenase/Δ5→4-isomerase type 1 (3β-HSD1), 16β-hydroxydehydroepiandrosterone |
| 3β-hydroxysteroid dehydrogenase/Δ5→4-isomerase type 1 (3β-HSD1) | Homo sapiens | androst-5-ene-3,17-dione | — | — | 30 | pH 7.4, 27 °C | Open record: 3β-hydroxysteroid dehydrogenase/Δ5→4-isomerase type 1 (3β-HSD1), androst-5-ene-3,17-dione |
| 3β-hydroxysteroid dehydrogenase/Δ5→4-isomerase type 1 (3β-HSD1)variant D61N | Homo sapiens | androst-5-ene-3,17-dione | 0.833 | 0.0288 | 29 | pH 7.4, 27 °C | Open record: 3β-hydroxysteroid dehydrogenase/Δ5→4-isomerase type 1 (3β-HSD1) variant D61N, androst-5-ene-3,17-dione |
| 3β-hydroxysteroid dehydrogenase/Δ5→4-isomerase type 1 (3β-HSD1)variant D61N | Homo sapiens | dehydroepiandrosterone | 0.047 | 0.0047 | 10 | pH 7.4, 27 °C | Open record: 3β-hydroxysteroid dehydrogenase/Δ5→4-isomerase type 1 (3β-HSD1) variant D61N, dehydroepiandrosterone |
| 3β-hydroxysteroid dehydrogenase/Δ5→4-isomerase type 1 (3β-HSD1)variant D61N | Homo sapiens | NAD+ | 0.062 | 0.0643 | 1 | pH 7.4, 27 °C | Open record: 3β-hydroxysteroid dehydrogenase/Δ5→4-isomerase type 1 (3β-HSD1) variant D61N, NAD+ |
| 3β-hydroxysteroid dehydrogenase/Δ5→4-isomerase type 1 (3β-HSD1)variant E192A | Homo sapiens | androst-5-ene-3,17-dione | 0.282 | 0.0198 | 14.2 | pH 7.4, 27 °C | Open record: 3β-hydroxysteroid dehydrogenase/Δ5→4-isomerase type 1 (3β-HSD1) variant E192A, androst-5-ene-3,17-dione |
| 3β-hydroxysteroid dehydrogenase/Δ5→4-isomerase type 1 (3β-HSD1)variant E192A | Homo sapiens | dehydroepiandrosterone | 0.03 | 0.0304 | 1 | pH 7.4, 27 °C | Open record: 3β-hydroxysteroid dehydrogenase/Δ5→4-isomerase type 1 (3β-HSD1) variant E192A, dehydroepiandrosterone |
| 3β-hydroxysteroid dehydrogenase/Δ5→4-isomerase type 1 (3β-HSD1)variant E192A | Homo sapiens | NAD+ | 0.027 | 0.0351 | 0.83 | pH 7.4, 27 °C | Open record: 3β-hydroxysteroid dehydrogenase/Δ5→4-isomerase type 1 (3β-HSD1) variant E192A, NAD+ |
| 3β-hydroxysteroid dehydrogenase/Δ5→4-isomerase type 1 (3β-HSD1)variant R195P | Homo sapiens | 16α-hydroxydehydroepiandrosterone | 0.155 | 0.0196 | 7.8 | pH 7.4, 27 °C | Open record: 3β-hydroxysteroid dehydrogenase/Δ5→4-isomerase type 1 (3β-HSD1) variant R195P, 16α-hydroxydehydroepiandrosterone |
| 3β-hydroxysteroid dehydrogenase/Δ5→4-isomerase type 1 (3β-HSD1)variant R195P | Homo sapiens | 16β-hydroxydehydroepiandrosterone | 0.155 | 0.015 | 10.3 | pH 7.4, 27 °C | Open record: 3β-hydroxysteroid dehydrogenase/Δ5→4-isomerase type 1 (3β-HSD1) variant R195P, 16β-hydroxydehydroepiandrosterone |
| 3β-hydroxysteroid dehydrogenase/Δ5→4-isomerase type 1 (3β-HSD1)variant R195P | Homo sapiens | dehydroepiandrosterone | 0.128 | 0.0107 | 12 | pH 7.4, 27 °C | Open record: 3β-hydroxysteroid dehydrogenase/Δ5→4-isomerase type 1 (3β-HSD1) variant R195P, dehydroepiandrosterone |
| 3β-hydroxysteroid dehydrogenase/Δ5→4-isomerase type 1 (3β-HSD1)variant R195P | Homo sapiens | NAD+ | 0.1 | 0.0205 | 4.8 | pH 7.4, 27 °C | Open record: 3β-hydroxysteroid dehydrogenase/Δ5→4-isomerase type 1 (3β-HSD1) variant R195P, NAD+ |
| 3β-hydroxysteroid dehydrogenase/Δ5→4-isomerase type 1 (3β-HSD1)variant T8A | Homo sapiens | androst-5-ene-3,17-dione | 0.74 | 0.0279 | 26.5 | pH 7.4, 27 °C | Open record: 3β-hydroxysteroid dehydrogenase/Δ5→4-isomerase type 1 (3β-HSD1) variant T8A, androst-5-ene-3,17-dione |
| 3β-hydroxysteroid dehydrogenase/Δ5→4-isomerase type 1 (3β-HSD1)variant T8A | Homo sapiens | dehydroepiandrosterone | 0.06 | 0.0082 | 7.3 | pH 7.4, 27 °C | Open record: 3β-hydroxysteroid dehydrogenase/Δ5→4-isomerase type 1 (3β-HSD1) variant T8A, dehydroepiandrosterone |
| 3β-hydroxysteroid dehydrogenase/Δ5→4-isomerase type 1 (3β-HSD1)variant T8A | Homo sapiens | NAD+ | 0.045 | 0.0333 | 1.3 | pH 7.4, 27 °C | Open record: 3β-hydroxysteroid dehydrogenase/Δ5→4-isomerase type 1 (3β-HSD1) variant T8A, NAD+ |
| 3β-hydroxysteroid dehydrogenase/Δ5→4-isomerase type 2 (3β-HSD2) | Homo sapiens | 16α-hydroxydehydroepiandrosterone | 0.152 | 0.0148 | 10.2 | pH 7.4, 27 °C | Open record: 3β-hydroxysteroid dehydrogenase/Δ5→4-isomerase type 2 (3β-HSD2), 16α-hydroxydehydroepiandrosterone |
| 3β-hydroxysteroid dehydrogenase/Δ5→4-isomerase type 2 (3β-HSD2) | Homo sapiens | 16β-hydroxydehydroepiandrosterone | 0.14 | 0.0184 | 7.7 | pH 7.4, 27 °C | Open record: 3β-hydroxysteroid dehydrogenase/Δ5→4-isomerase type 2 (3β-HSD2), 16β-hydroxydehydroepiandrosterone |
| 3β-hydroxysteroid dehydrogenase/Δ5→4-isomerase type 2 (3β-HSD2) | Homo sapiens | dehydroepiandrosterone | 0.145 | 0.0156 | 7.3 | pH 7.4, 27 °C | Open record: 3β-hydroxysteroid dehydrogenase/Δ5→4-isomerase type 2 (3β-HSD2), dehydroepiandrosterone |
| 3β-hydroxysteroid dehydrogenase/Δ5→4-isomerase type 2 (3β-HSD2)variant P195R | Homo sapiens | 16α-hydroxydehydroepiandrosterone | 0.077 | 0.004 | 19.2 | pH 7.4, 27 °C | Open record: 3β-hydroxysteroid dehydrogenase/Δ5→4-isomerase type 2 (3β-HSD2) variant P195R, 16α-hydroxydehydroepiandrosterone |
| 3β-hydroxysteroid dehydrogenase/Δ5→4-isomerase type 2 (3β-HSD2)variant P195R | Homo sapiens | 16β-hydroxydehydroepiandrosterone | 0.053 | 0.0015 | 35.5 | pH 7.4, 27 °C | Open record: 3β-hydroxysteroid dehydrogenase/Δ5→4-isomerase type 2 (3β-HSD2) variant P195R, 16β-hydroxydehydroepiandrosterone |
| 3β-hydroxysteroid dehydrogenase/Δ5→4-isomerase type 2 (3β-HSD2)variant P195R | Homo sapiens | dehydroepiandrosterone | 0.093 | 0.0077 | 12.2 | pH 7.4, 27 °C | Open record: 3β-hydroxysteroid dehydrogenase/Δ5→4-isomerase type 2 (3β-HSD2) variant P195R, dehydroepiandrosterone |
| 3β-hydroxysteroid dehydrogenase/Δ5→4-isomerase type 2 (3β-HSD2)variant P195R | Homo sapiens | NAD+ | 0.175 | 0.0564 | 3.2 | pH 7.4, 27 °C | Open record: 3β-hydroxysteroid dehydrogenase/Δ5→4-isomerase type 2 (3β-HSD2) variant P195R, NAD+ |