Values CatLog read from this paper
Identification and characterization of important residues in the catalytic mechanism of CMP-Neu5Ac synthetase from Neisseria meningitidis
PMID 20491913doi:10.1111/j.1742-4658.2010.07696.xOpen in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 28 records (69 values) from this paper: CMP-Neu5Ac synthetase (CNS) from Neisseria meningitidis.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 1 kcat/Km value marked “calc.” from kcat and Km; no printed kcat/Km was read for that record.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| CMP-Neu5Ac synthetase (CNS) | Neisseria meningitidis | cytidine 5'-triphosphate | 10.17 | 0.017 | 600 | pH 7.5, 22 °C | Open record: CMP-Neu5Ac synthetase (CNS), cytidine 5'-triphosphate |
| CMP-Neu5Ac synthetase (CNS) | Neisseria meningitidis | cytidine 5'-triphosphateapparent parameters | 9 | 0.034 | 270 | pH 7.5, 22 °C | Open record: CMP-Neu5Ac synthetase (CNS), cytidine 5'-triphosphate |
| CMP-Neu5Ac synthetase (CNS) | Neisseria meningitidis | N-acetylneuraminic acid | — | 0.068 | 148 | pH 7.5, 22 °C | Open record: CMP-Neu5Ac synthetase (CNS), N-acetylneuraminic acid |
| CMP-Neu5Ac synthetase (CNS) | Neisseria meningitidis | N-acetylneuraminic acidapparent parameters | 9 | 0.13 | 68 | pH 7.5, 22 °C | Open record: CMP-Neu5Ac synthetase (CNS), N-acetylneuraminic acid |
| CMP-Neu5Ac synthetase (CNS) | Neisseria meningitidis | N-acetylneuraminic acidwith 0.2 mM dithiothreitol; apparent parameters | 9.3 | 0.19 | 49 calc. | pH 7.5, 22 °C | Open record: CMP-Neu5Ac synthetase (CNS), N-acetylneuraminic acid |
| CMP-Neu5Ac synthetase (CNS)variant D211A | Neisseria meningitidis | cytidine 5'-triphosphate | 6.8 × 10⁻⁴ | 0.026 | 0.027 | pH 7.5, 22 °C | Open record: CMP-Neu5Ac synthetase (CNS) variant D211A, cytidine 5'-triphosphate |
| CMP-Neu5Ac synthetase (CNS)variant D211A | Neisseria meningitidis | N-acetylneuraminic acid | — | 0.066 | 0.0103 | pH 7.5, 22 °C | Open record: CMP-Neu5Ac synthetase (CNS) variant D211A, N-acetylneuraminic acid |
| CMP-Neu5Ac synthetase (CNS)variant F192A | Neisseria meningitidis | cytidine 5'-triphosphate | 20 | 0.15 | 132 | pH 7.5, 22 °C | Open record: CMP-Neu5Ac synthetase (CNS) variant F192A, cytidine 5'-triphosphate |
| CMP-Neu5Ac synthetase (CNS)variant F192A | Neisseria meningitidis | N-acetylneuraminic acid | — | 0.38 | 52 | pH 7.5, 22 °C | Open record: CMP-Neu5Ac synthetase (CNS) variant F192A, N-acetylneuraminic acid |
| CMP-Neu5Ac synthetase (CNS)variant F193A | Neisseria meningitidis | cytidine 5'-triphosphate | 5 | 0.052 | 97 | pH 7.5, 22 °C | Open record: CMP-Neu5Ac synthetase (CNS) variant F193A, cytidine 5'-triphosphate |
| CMP-Neu5Ac synthetase (CNS)variant F193A | Neisseria meningitidis | N-acetylneuraminic acid | — | 0.78 | 6.3 | pH 7.5, 22 °C | Open record: CMP-Neu5Ac synthetase (CNS) variant F193A, N-acetylneuraminic acid |
| CMP-Neu5Ac synthetase (CNS)variant K142A | Neisseria meningitidis | cytidine 5'-triphosphate | 9.3 × 10⁻⁴ | 0.14 | 0.0063 | pH 7.5, 22 °C | Open record: CMP-Neu5Ac synthetase (CNS) variant K142A, cytidine 5'-triphosphate |
| CMP-Neu5Ac synthetase (CNS)variant K142A | Neisseria meningitidis | N-acetylneuraminic acid | — | — | 6 × 10⁻⁴ | pH 7.5, 22 °C | Open record: CMP-Neu5Ac synthetase (CNS) variant K142A, N-acetylneuraminic acid |
| CMP-Neu5Ac synthetase (CNS)variant N175A | Neisseria meningitidis | cytidine 5'-triphosphate | 25 | 0.27 | 90 | pH 7.5, 22 °C | Open record: CMP-Neu5Ac synthetase (CNS) variant N175A, cytidine 5'-triphosphate |
| CMP-Neu5Ac synthetase (CNS)variant N175A | Neisseria meningitidis | N-acetylneuraminic acid | — | 1.6 | 15.5 | pH 7.5, 22 °C | Open record: CMP-Neu5Ac synthetase (CNS) variant N175A, N-acetylneuraminic acid |
| CMP-Neu5Ac synthetase (CNS)variant Q104A | Neisseria meningitidis | cytidine 5'-triphosphate | 2.17 | 0.031 | 72 | pH 7.5, 22 °C | Open record: CMP-Neu5Ac synthetase (CNS) variant Q104A, cytidine 5'-triphosphate |
| CMP-Neu5Ac synthetase (CNS)variant Q104A | Neisseria meningitidis | N-acetylneuraminic acid | — | 2.6 | 0.85 | pH 7.5, 22 °C | Open record: CMP-Neu5Ac synthetase (CNS) variant Q104A, N-acetylneuraminic acid |
| CMP-Neu5Ac synthetase (CNS)variant Q104A | Neisseria meningitidis | N-acetylneuraminic acidapparent parameters | 1.83 | 4.7 | 0.4 | pH 7.5, 22 °C | Open record: CMP-Neu5Ac synthetase (CNS) variant Q104A, N-acetylneuraminic acid |
| CMP-Neu5Ac synthetase (CNS)variant Q104E | Neisseria meningitidis | cytidine 5'-triphosphate | 7.7 × 10⁻⁴ | 0.2 | 0.0038 | pH 7.5, 22 °C | Open record: CMP-Neu5Ac synthetase (CNS) variant Q104E, cytidine 5'-triphosphate |
| CMP-Neu5Ac synthetase (CNS)variant Q104E | Neisseria meningitidis | N-acetylneuraminic acid | 4.5 × 10⁻⁴ | 0.31 | 0.00145 | pH 7.5, 22 °C | Open record: CMP-Neu5Ac synthetase (CNS) variant Q104E, N-acetylneuraminic acid |
| CMP-Neu5Ac synthetase (CNS)variant Q104L | Neisseria meningitidis | cytidine 5'-triphosphate | 0.0028 | 0.15 | 0.018 | pH 7.5, 22 °C | Open record: CMP-Neu5Ac synthetase (CNS) variant Q104L, cytidine 5'-triphosphate |
| CMP-Neu5Ac synthetase (CNS)variant Q104L | Neisseria meningitidis | N-acetylneuraminic acid | — | — | 0.0048 | pH 7.5, 22 °C | Open record: CMP-Neu5Ac synthetase (CNS) variant Q104L, N-acetylneuraminic acid |
| CMP-Neu5Ac synthetase (CNS)variant Q104N | Neisseria meningitidis | cytidine 5'-triphosphate | 0.0123 | 0.032 | 0.38 | pH 7.5, 22 °C | Open record: CMP-Neu5Ac synthetase (CNS) variant Q104N, cytidine 5'-triphosphate |
| CMP-Neu5Ac synthetase (CNS)variant Q104N | Neisseria meningitidis | N-acetylneuraminic acid | — | — | 0.0125 | pH 7.5, 22 °C | Open record: CMP-Neu5Ac synthetase (CNS) variant Q104N, N-acetylneuraminic acid |
| CMP-Neu5Ac synthetase (CNS)variant R173A | Neisseria meningitidis | cytidine 5'-triphosphate | 7 | 0.048 | 143 | pH 7.5, 22 °C | Open record: CMP-Neu5Ac synthetase (CNS) variant R173A, cytidine 5'-triphosphate |
| CMP-Neu5Ac synthetase (CNS)variant R173A | Neisseria meningitidis | N-acetylneuraminic acid | — | 0.29 | 23 | pH 7.5, 22 °C | Open record: CMP-Neu5Ac synthetase (CNS) variant R173A, N-acetylneuraminic acid |
| CMP-Neu5Ac synthetase (CNS)variant Y179A | Neisseria meningitidis | cytidine 5'-triphosphate | 0.052 | 0.04 | 1.28 | pH 7.5, 22 °C | Open record: CMP-Neu5Ac synthetase (CNS) variant Y179A, cytidine 5'-triphosphate |
| CMP-Neu5Ac synthetase (CNS)variant Y179A | Neisseria meningitidis | N-acetylneuraminic acid | — | 0.11 | 0.47 | pH 7.5, 22 °C | Open record: CMP-Neu5Ac synthetase (CNS) variant Y179A, N-acetylneuraminic acid |