Values CatLog read from this paper
The functional role of cysteines in isopenicillin N synthase. Correlation of cysteine reactivities toward sulfhydryl reagents with kinetic properties of cysteine mutants
PMID 2050677doi:10.1016/s0021-9258(18)99025-3Open in Browse
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CatLog read 5 records (15 values) from this paper: isopenicillin N synthase (IPNS) from Cephalosporium acremonium.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| isopenicillin N synthase (IPNS) | Cephalosporium acremonium | delta-(L-alpha-aminoadipoyl)-L-cysteinyl-D-valine | 3.2 | 0.37 | 8.6 | pH 7.1, 25 °C | Open record: isopenicillin N synthase (IPNS), delta-(L-alpha-aminoadipoyl)-L-cysteinyl-D-valine |
| isopenicillin N synthase (IPNS) | Cephalosporium acremonium | delta-(L-alpha-aminoadipoyl)-L-cysteinyl-D-valinerecombinant | 4.6 | 0.56 | 8.2 | pH 7.1, 25 °C | Open record: isopenicillin N synthase (IPNS), delta-(L-alpha-aminoadipoyl)-L-cysteinyl-D-valine |
| isopenicillin N synthase (IPNS)variant C106S | Cephalosporium acremonium | delta-(L-alpha-aminoadipoyl)-L-cysteinyl-D-valine | 1.7 | 7.8 | 0.2 | pH 7.1, 25 °C | Open record: isopenicillin N synthase (IPNS) variant C106S, delta-(L-alpha-aminoadipoyl)-L-cysteinyl-D-valine |
| isopenicillin N synthase (IPNS)variant C106S/C255S | Cephalosporium acremonium | delta-(L-alpha-aminoadipoyl)-L-cysteinyl-D-valine | 1.7 | 7.82 | 0.2 | pH 7.1, 25 °C | Open record: isopenicillin N synthase (IPNS) variant C106S/C255S, delta-(L-alpha-aminoadipoyl)-L-cysteinyl-D-valine |
| isopenicillin N synthase (IPNS)variant C255S | Cephalosporium acremonium | delta-(L-alpha-aminoadipoyl)-L-cysteinyl-D-valine | 3.1 | 0.78 | 4 | pH 7.1, 25 °C | Open record: isopenicillin N synthase (IPNS) variant C255S, delta-(L-alpha-aminoadipoyl)-L-cysteinyl-D-valine |