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Structure and reactivity of Bacillus subtilis MenD catalyzing the first committed step in menaquinone biosynthesis

PMID 20600129doi:10.1016/j.jmb.2010.06.025Open in Browse

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CatLog read 18 records (54 values) from this paper: 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexadiene-1-carboxylate synthase (MenD, SEPHCHC synthase) from Bacillus subtilis.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.

EC 2.2.1.9

Records read from this paper
Enzyme	Organism	Substrate	k_cat (s⁻¹)	K_m (mM)	k_cat/K_m (mM⁻¹ s⁻¹)	Conditions	Open record
2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexadiene-1-carboxylate synthase (MenD, SEPHCHC synthase)	Bacillus subtilis	isochorismate	0.32	0.0011	320	pH 7.8, 23 °C	Open record: 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexadiene-1-carboxylate synthase (MenD, SEPHCHC synthase), isochorismate
2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexadiene-1-carboxylate synthase (MenD, SEPHCHC synthase)	Bacillus subtilis	α-ketoglutarate	0.23	0.022	10.7	pH 7.8, 23 °C	Open record: 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexadiene-1-carboxylate synthase (MenD, SEPHCHC synthase), α-ketoglutarate
2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexadiene-1-carboxylate synthase (MenD, SEPHCHC synthase)variant F490A	Bacillus subtilis	isochorismate	0.028	0.029	0.97	pH 7.8, 23 °C	Open record: 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexadiene-1-carboxylate synthase (MenD, SEPHCHC synthase) variant F490A, isochorismate
2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexadiene-1-carboxylate synthase (MenD, SEPHCHC synthase)variant F490A	Bacillus subtilis	α-ketoglutarate	0.018	0.274	0.065	pH 7.8, 23 °C	Open record: 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexadiene-1-carboxylate synthase (MenD, SEPHCHC synthase) variant F490A, α-ketoglutarate
2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexadiene-1-carboxylate synthase (MenD, SEPHCHC synthase)variant I489A	Bacillus subtilis	isochorismate	0.095	0.027	3.5	pH 7.8, 23 °C	Open record: 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexadiene-1-carboxylate synthase (MenD, SEPHCHC synthase) variant I489A, isochorismate
2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexadiene-1-carboxylate synthase (MenD, SEPHCHC synthase)variant I489A	Bacillus subtilis	α-ketoglutarate	0.095	0.346	52	pH 7.8, 23 °C	Open record: 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexadiene-1-carboxylate synthase (MenD, SEPHCHC synthase) variant I489A, α-ketoglutarate
2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexadiene-1-carboxylate synthase (MenD, SEPHCHC synthase)variant K299A	Bacillus subtilis	isochorismate	0.42	0.0084	50	pH 7.8, 23 °C	Open record: 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexadiene-1-carboxylate synthase (MenD, SEPHCHC synthase) variant K299A, isochorismate
2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexadiene-1-carboxylate synthase (MenD, SEPHCHC synthase)variant K299A	Bacillus subtilis	α-ketoglutarate	0.37	0.022	17	pH 7.8, 23 °C	Open record: 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexadiene-1-carboxylate synthase (MenD, SEPHCHC synthase) variant K299A, α-ketoglutarate
2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexadiene-1-carboxylate synthase (MenD, SEPHCHC synthase)variant L493A	Bacillus subtilis	isochorismate	0.32	0.039	8.2	pH 7.8, 23 °C	Open record: 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexadiene-1-carboxylate synthase (MenD, SEPHCHC synthase) variant L493A, isochorismate
2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexadiene-1-carboxylate synthase (MenD, SEPHCHC synthase)variant L493A	Bacillus subtilis	α-ketoglutarate	0.17	0.018	8.3	pH 7.8, 23 °C	Open record: 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexadiene-1-carboxylate synthase (MenD, SEPHCHC synthase) variant L493A, α-ketoglutarate
2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexadiene-1-carboxylate synthase (MenD, SEPHCHC synthase)variant R106A	Bacillus subtilis	isochorismate	0.012	0.041	0.3	pH 7.8, 23 °C	Open record: 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexadiene-1-carboxylate synthase (MenD, SEPHCHC synthase) variant R106A, isochorismate
2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexadiene-1-carboxylate synthase (MenD, SEPHCHC synthase)variant R106A	Bacillus subtilis	α-ketoglutarate	0.01	0.0077	1.5	pH 7.8, 23 °C	Open record: 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexadiene-1-carboxylate synthase (MenD, SEPHCHC synthase) variant R106A, α-ketoglutarate
2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexadiene-1-carboxylate synthase (MenD, SEPHCHC synthase)variant R32A	Bacillus subtilis	isochorismate	0.18	0.049	3.7	pH 7.8, 23 °C	Open record: 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexadiene-1-carboxylate synthase (MenD, SEPHCHC synthase) variant R32A, isochorismate
2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexadiene-1-carboxylate synthase (MenD, SEPHCHC synthase)variant R32A	Bacillus subtilis	α-ketoglutarate	0.08	0.022	3.7	pH 7.8, 23 °C	Open record: 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexadiene-1-carboxylate synthase (MenD, SEPHCHC synthase) variant R32A, α-ketoglutarate
2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexadiene-1-carboxylate synthase (MenD, SEPHCHC synthase)variant R409A	Bacillus subtilis	isochorismate	0.023	0.0085	2.7	pH 7.8, 23 °C	Open record: 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexadiene-1-carboxylate synthase (MenD, SEPHCHC synthase) variant R409A, isochorismate
2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexadiene-1-carboxylate synthase (MenD, SEPHCHC synthase)variant R409A	Bacillus subtilis	α-ketoglutarate	0.045	0.159	2.7	pH 7.8, 23 °C	Open record: 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexadiene-1-carboxylate synthase (MenD, SEPHCHC synthase) variant R409A, α-ketoglutarate
2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexadiene-1-carboxylate synthase (MenD, SEPHCHC synthase)variant R428A	Bacillus subtilis	isochorismate	0.047	0.0011	47	pH 7.8, 23 °C	Open record: 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexadiene-1-carboxylate synthase (MenD, SEPHCHC synthase) variant R428A, isochorismate
2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexadiene-1-carboxylate synthase (MenD, SEPHCHC synthase)variant R428A	Bacillus subtilis	α-ketoglutarate	0.057	0.514	0.12	pH 7.8, 23 °C	Open record: 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexadiene-1-carboxylate synthase (MenD, SEPHCHC synthase) variant R428A, α-ketoglutarate