EC 2.2.1.9: 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylic-acid synthase
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CatLog read 73 records for EC 2.2.1.9 from 3 papers, for 2 organisms. Names in these papers: MenD (2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC) synthase), 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexadiene-1-carboxylate synthase (MenD, SEPHCHC synthase), MenD (SEPHCHC synthase).
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units.
CatLog also holds 6 records for EC 2.2.1.9 that are not yet re-read: from its earlier version, built from the enzyme databases and partly checked against the papers. All records for EC 2.2.1.9 in Browse.
Papers
| Paper | Organisms | Records |
|---|---|---|
| Using substrate analogues to probe the kinetic mechanism and active site of Escherichia coli MenD | Escherichia coli | 45 |
| Structure and reactivity of Bacillus subtilis MenD catalyzing the first committed step in menaquinone biosynthesis | Bacillus subtilis | 18 |
| Two active site arginines are critical determinants of substrate binding and catalysis in MenD: a thiamine-dependent enzyme in menaquinone biosynthesis | Escherichia coli | 10 |
Records
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Paper | Open record |
|---|---|---|---|---|---|---|---|---|
| 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexadiene-1-carboxylate synthase (MenD, SEPHCHC synthase) | Bacillus subtilis | isochorismate | 0.32 | 0.0011 | 320 | pH 7.8, 23 °C | PMID 20600129 | Open record: 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexadiene-1-carboxylate synthase (MenD, SEPHCHC synthase), isochorismate |
| 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexadiene-1-carboxylate synthase (MenD, SEPHCHC synthase) | Bacillus subtilis | α-ketoglutarate | 0.23 | 0.022 | 10.7 | pH 7.8, 23 °C | PMID 20600129 | Open record: 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexadiene-1-carboxylate synthase (MenD, SEPHCHC synthase), α-ketoglutarate |
| 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexadiene-1-carboxylate synthase (MenD, SEPHCHC synthase)variant F490A | Bacillus subtilis | isochorismate | 0.028 | 0.029 | 0.97 | pH 7.8, 23 °C | PMID 20600129 | Open record: 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexadiene-1-carboxylate synthase (MenD, SEPHCHC synthase) variant F490A, isochorismate |
| 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexadiene-1-carboxylate synthase (MenD, SEPHCHC synthase)variant F490A | Bacillus subtilis | α-ketoglutarate | 0.018 | 0.274 | 0.065 | pH 7.8, 23 °C | PMID 20600129 | Open record: 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexadiene-1-carboxylate synthase (MenD, SEPHCHC synthase) variant F490A, α-ketoglutarate |
| 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexadiene-1-carboxylate synthase (MenD, SEPHCHC synthase)variant I489A | Bacillus subtilis | isochorismate | 0.095 | 0.027 | 3.5 | pH 7.8, 23 °C | PMID 20600129 | Open record: 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexadiene-1-carboxylate synthase (MenD, SEPHCHC synthase) variant I489A, isochorismate |
| 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexadiene-1-carboxylate synthase (MenD, SEPHCHC synthase)variant I489A | Bacillus subtilis | α-ketoglutarate | 0.095 | 0.346 | 52 | pH 7.8, 23 °C | PMID 20600129 | Open record: 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexadiene-1-carboxylate synthase (MenD, SEPHCHC synthase) variant I489A, α-ketoglutarate |
| 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexadiene-1-carboxylate synthase (MenD, SEPHCHC synthase)variant K299A | Bacillus subtilis | isochorismate | 0.42 | 0.0084 | 50 | pH 7.8, 23 °C | PMID 20600129 | Open record: 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexadiene-1-carboxylate synthase (MenD, SEPHCHC synthase) variant K299A, isochorismate |
| 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexadiene-1-carboxylate synthase (MenD, SEPHCHC synthase)variant K299A | Bacillus subtilis | α-ketoglutarate | 0.37 | 0.022 | 17 | pH 7.8, 23 °C | PMID 20600129 | Open record: 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexadiene-1-carboxylate synthase (MenD, SEPHCHC synthase) variant K299A, α-ketoglutarate |
| 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexadiene-1-carboxylate synthase (MenD, SEPHCHC synthase)variant L493A | Bacillus subtilis | isochorismate | 0.32 | 0.039 | 8.2 | pH 7.8, 23 °C | PMID 20600129 | Open record: 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexadiene-1-carboxylate synthase (MenD, SEPHCHC synthase) variant L493A, isochorismate |
| 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexadiene-1-carboxylate synthase (MenD, SEPHCHC synthase)variant L493A | Bacillus subtilis | α-ketoglutarate | 0.17 | 0.018 | 8.3 | pH 7.8, 23 °C | PMID 20600129 | Open record: 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexadiene-1-carboxylate synthase (MenD, SEPHCHC synthase) variant L493A, α-ketoglutarate |
| 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexadiene-1-carboxylate synthase (MenD, SEPHCHC synthase)variant R106A | Bacillus subtilis | isochorismate | 0.012 | 0.041 | 0.3 | pH 7.8, 23 °C | PMID 20600129 | Open record: 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexadiene-1-carboxylate synthase (MenD, SEPHCHC synthase) variant R106A, isochorismate |
| 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexadiene-1-carboxylate synthase (MenD, SEPHCHC synthase)variant R106A | Bacillus subtilis | α-ketoglutarate | 0.01 | 0.0077 | 1.5 | pH 7.8, 23 °C | PMID 20600129 | Open record: 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexadiene-1-carboxylate synthase (MenD, SEPHCHC synthase) variant R106A, α-ketoglutarate |
| 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexadiene-1-carboxylate synthase (MenD, SEPHCHC synthase)variant R32A | Bacillus subtilis | isochorismate | 0.18 | 0.049 | 3.7 | pH 7.8, 23 °C | PMID 20600129 | Open record: 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexadiene-1-carboxylate synthase (MenD, SEPHCHC synthase) variant R32A, isochorismate |
| 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexadiene-1-carboxylate synthase (MenD, SEPHCHC synthase)variant R32A | Bacillus subtilis | α-ketoglutarate | 0.08 | 0.022 | 3.7 | pH 7.8, 23 °C | PMID 20600129 | Open record: 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexadiene-1-carboxylate synthase (MenD, SEPHCHC synthase) variant R32A, α-ketoglutarate |
| 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexadiene-1-carboxylate synthase (MenD, SEPHCHC synthase)variant R409A | Bacillus subtilis | isochorismate | 0.023 | 0.0085 | 2.7 | pH 7.8, 23 °C | PMID 20600129 | Open record: 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexadiene-1-carboxylate synthase (MenD, SEPHCHC synthase) variant R409A, isochorismate |
| 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexadiene-1-carboxylate synthase (MenD, SEPHCHC synthase)variant R409A | Bacillus subtilis | α-ketoglutarate | 0.045 | 0.159 | 2.7 | pH 7.8, 23 °C | PMID 20600129 | Open record: 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexadiene-1-carboxylate synthase (MenD, SEPHCHC synthase) variant R409A, α-ketoglutarate |
| 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexadiene-1-carboxylate synthase (MenD, SEPHCHC synthase)variant R428A | Bacillus subtilis | isochorismate | 0.047 | 0.0011 | 47 | pH 7.8, 23 °C | PMID 20600129 | Open record: 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexadiene-1-carboxylate synthase (MenD, SEPHCHC synthase) variant R428A, isochorismate |
| 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexadiene-1-carboxylate synthase (MenD, SEPHCHC synthase)variant R428A | Bacillus subtilis | α-ketoglutarate | 0.057 | 0.514 | 0.12 | pH 7.8, 23 °C | PMID 20600129 | Open record: 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexadiene-1-carboxylate synthase (MenD, SEPHCHC synthase) variant R428A, α-ketoglutarate |
| MenD (2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC) synthase) | Escherichia coli | (+)-trans-5-(carboxylatomethoxy)-6-hydroxycyclohexa-1,3-diene carboxylate | 0.45 | 0.0091 | 50 | pH 7.4 | PMID 21928762 | Open record: MenD (2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC) synthase), (+)-trans-5-(carboxylatomethoxy)-6-hydroxycyclohexa-1,3-diene carboxylate |
| MenD (2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC) synthase) | Escherichia coli | (+)-trans-5-(carboxylatomethoxy)-6-hydroxycyclohexa-1,3-diene carboxylate | 0.4 | 0.012 | 33 | PMID 21928762 | Open record: MenD (2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC) synthase), (+)-trans-5-(carboxylatomethoxy)-6-hydroxycyclohexa-1,3-diene carboxylate | |
| MenD (2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC) synthase) | Escherichia coli | isochorismate | 0.28 | — | — | PMID 21928762 | Open record: MenD (2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC) synthase), isochorismate | |
| MenD (2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC) synthase) | Escherichia coli | thiamine diphosphate | 0.28 | 0.008 | 35 | PMID 21928762 | Open record: MenD (2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC) synthase), thiamine diphosphate | |
| MenD (2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC) synthase) | Escherichia coli | α-ketoglutarate | — | 0.0038 | 120 | pH 7.4 | PMID 21928762 | Open record: MenD (2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC) synthase), α-ketoglutarate |
| MenD (2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC) synthase) | Escherichia coli | α-ketoglutarate | 0.28 | 0.0099 | 28 | PMID 21928762 | Open record: MenD (2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC) synthase), α-ketoglutarate | |
| MenD (2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC) synthase)variant E55D | Escherichia coli | thiamine diphosphate | 0.22 | 0.024 | 8.8 | PMID 21928762 | Open record: MenD (2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC) synthase) variant E55D, thiamine diphosphate | |
| MenD (2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC) synthase)variant E55D | Escherichia coli | α-ketoglutarate | 0.22 | 0.012 | 18 | PMID 21928762 | Open record: MenD (2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC) synthase) variant E55D, α-ketoglutarate | |
| MenD (2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC) synthase)variant I418L | Escherichia coli | (+)-trans-5-(carboxylatomethoxy)-6-hydroxycyclohexa-1,3-diene carboxylate | 0.38 | 0.027 | 14.2 | PMID 21928762 | Open record: MenD (2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC) synthase) variant I418L, (+)-trans-5-(carboxylatomethoxy)-6-hydroxycyclohexa-1,3-diene carboxylate | |
| MenD (2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC) synthase)variant I418L | Escherichia coli | isochorismate | 0.22 | — | — | PMID 21928762 | Open record: MenD (2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC) synthase) variant I418L, isochorismate | |
| MenD (2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC) synthase)variant I418L | Escherichia coli | α-ketoglutarate | 0.22 | 0.2 | 1.05 | PMID 21928762 | Open record: MenD (2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC) synthase) variant I418L, α-ketoglutarate | |
| MenD (2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC) synthase)variant K292Q | Escherichia coli | (+)-trans-5-(carboxylatomethoxy)-6-hydroxycyclohexa-1,3-diene carboxylate | 0.28 | 0.05 | 5.7 | PMID 21928762 | Open record: MenD (2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC) synthase) variant K292Q, (+)-trans-5-(carboxylatomethoxy)-6-hydroxycyclohexa-1,3-diene carboxylate | |
| MenD (2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC) synthase)variant K292Q | Escherichia coli | isochorismate | 0.2 | — | — | PMID 21928762 | Open record: MenD (2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC) synthase) variant K292Q, isochorismate | |
| MenD (2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC) synthase)variant K292Q | Escherichia coli | thiamine diphosphate | 0.2 | 0.002 | 100 | PMID 21928762 | Open record: MenD (2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC) synthase) variant K292Q, thiamine diphosphate | |
| MenD (2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC) synthase)variant K292Q | Escherichia coli | α-ketoglutarate | 0.2 | 0.0026 | 77 | PMID 21928762 | Open record: MenD (2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC) synthase) variant K292Q, α-ketoglutarate | |
| MenD (2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC) synthase)variant R107K | Escherichia coli | (+)-trans-5-(carboxylatomethoxy)-6-hydroxycyclohexa-1,3-diene carboxylate | 0.118 | 0.091 | 1.3 | PMID 21928762 | Open record: MenD (2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC) synthase) variant R107K, (+)-trans-5-(carboxylatomethoxy)-6-hydroxycyclohexa-1,3-diene carboxylate | |
| MenD (2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC) synthase)variant R107K | Escherichia coli | isochorismate | 0.17 | 0.0075 | 22 | PMID 21928762 | Open record: MenD (2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC) synthase) variant R107K, isochorismate | |
| MenD (2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC) synthase)variant R107K | Escherichia coli | thiamine diphosphate | 0.17 | 0.0048 | 37 | PMID 21928762 | Open record: MenD (2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC) synthase) variant R107K, thiamine diphosphate | |
| MenD (2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC) synthase)variant R107K | Escherichia coli | α-ketoglutarate | 0.17 | 0.0053 | 32 | PMID 21928762 | Open record: MenD (2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC) synthase) variant R107K, α-ketoglutarate | |
| MenD (2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC) synthase)variant R293K | Escherichia coli | (+)-trans-5-(carboxylatomethoxy)-6-hydroxycyclohexa-1,3-diene carboxylate | 0.4 | 0.0042 | 95 | PMID 21928762 | Open record: MenD (2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC) synthase) variant R293K, (+)-trans-5-(carboxylatomethoxy)-6-hydroxycyclohexa-1,3-diene carboxylate | |
| MenD (2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC) synthase)variant R293K | Escherichia coli | isochorismate | 0.3 | — | — | PMID 21928762 | Open record: MenD (2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC) synthase) variant R293K, isochorismate | |
| MenD (2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC) synthase)variant R293K | Escherichia coli | thiamine diphosphate | 0.3 | 0.0047 | 63 | PMID 21928762 | Open record: MenD (2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC) synthase) variant R293K, thiamine diphosphate | |
| MenD (2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC) synthase)variant R293K | Escherichia coli | α-ketoglutarate | 0.3 | 0.0091 | 33 | PMID 21928762 | Open record: MenD (2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC) synthase) variant R293K, α-ketoglutarate | |
| MenD (2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC) synthase)variant R33K | Escherichia coli | isochorismate | 0.105 | 0.14 | 0.78 | PMID 21928762 | Open record: MenD (2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC) synthase) variant R33K, isochorismate | |
| MenD (2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC) synthase)variant R33Q | Escherichia coli | (+)-trans-5-(carboxylatomethoxy)-6-hydroxycyclohexa-1,3-diene carboxylate | 0.04 | 0.43 | 0.093 | PMID 21928762 | Open record: MenD (2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC) synthase) variant R33Q, (+)-trans-5-(carboxylatomethoxy)-6-hydroxycyclohexa-1,3-diene carboxylate | |
| MenD (2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC) synthase)variant R33Q | Escherichia coli | isochorismate | 0.122 | 0.054 | 2.3 | PMID 21928762 | Open record: MenD (2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC) synthase) variant R33Q, isochorismate | |
| MenD (2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC) synthase)variant R395A | Escherichia coli | (+)-trans-5-(carboxylatomethoxy)-6-hydroxycyclohexa-1,3-diene carboxylate | 0.04 | 0.39 | 0.102 | PMID 21928762 | Open record: MenD (2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC) synthase) variant R395A, (+)-trans-5-(carboxylatomethoxy)-6-hydroxycyclohexa-1,3-diene carboxylate | |
| MenD (2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC) synthase)variant R395A | Escherichia coli | isochorismate | 0.043 | 0.036 | 1.2 | PMID 21928762 | Open record: MenD (2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC) synthase) variant R395A, isochorismate | |
| MenD (2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC) synthase)variant R395K | Escherichia coli | (+)-trans-5-(carboxylatomethoxy)-6-hydroxycyclohexa-1,3-diene carboxylate | 0.18 | 0.109 | 1.7 | PMID 21928762 | Open record: MenD (2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC) synthase) variant R395K, (+)-trans-5-(carboxylatomethoxy)-6-hydroxycyclohexa-1,3-diene carboxylate | |
| MenD (2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC) synthase)variant R395K | Escherichia coli | isochorismate | 0.132 | 0.0053 | 25 | PMID 21928762 | Open record: MenD (2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC) synthase) variant R395K, isochorismate | |
| MenD (2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC) synthase)variant R395K | Escherichia coli | thiamine diphosphate | 0.132 | 0.0022 | 60 | PMID 21928762 | Open record: MenD (2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC) synthase) variant R395K, thiamine diphosphate | |
| MenD (2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC) synthase)variant R395K | Escherichia coli | α-ketoglutarate | 0.132 | 0.045 | 3 | PMID 21928762 | Open record: MenD (2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC) synthase) variant R395K, α-ketoglutarate | |
| MenD (2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC) synthase)variant R413K | Escherichia coli | (+)-trans-5-(carboxylatomethoxy)-6-hydroxycyclohexa-1,3-diene carboxylate | 0.17 | 0.01 | 17 | PMID 21928762 | Open record: MenD (2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC) synthase) variant R413K, (+)-trans-5-(carboxylatomethoxy)-6-hydroxycyclohexa-1,3-diene carboxylate | |
| MenD (2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC) synthase)variant R413K | Escherichia coli | isochorismate | 0.22 | — | — | PMID 21928762 | Open record: MenD (2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC) synthase) variant R413K, isochorismate | |
| MenD (2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC) synthase)variant R413K | Escherichia coli | thiamine diphosphate | 0.102 | 0.005 | 20 | PMID 21928762 | Open record: MenD (2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC) synthase) variant R413K, thiamine diphosphate | |
| MenD (2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC) synthase)variant R413K | Escherichia coli | α-ketoglutarate | 0.102 | 0.22 | 0.47 | PMID 21928762 | Open record: MenD (2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC) synthase) variant R413K, α-ketoglutarate | |
| MenD (2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC) synthase)variant S32A | Escherichia coli | (+)-trans-5-(carboxylatomethoxy)-6-hydroxycyclohexa-1,3-diene carboxylate | 0.25 | 0.042 | 5.8 | PMID 21928762 | Open record: MenD (2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC) synthase) variant S32A, (+)-trans-5-(carboxylatomethoxy)-6-hydroxycyclohexa-1,3-diene carboxylate | |
| MenD (2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC) synthase)variant S32A | Escherichia coli | isochorismate | 0.23 | — | — | PMID 21928762 | Open record: MenD (2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC) synthase) variant S32A, isochorismate | |
| MenD (2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC) synthase)variant S32A | Escherichia coli | thiamine diphosphate | 0.23 | 0.0043 | 53 | PMID 21928762 | Open record: MenD (2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC) synthase) variant S32A, thiamine diphosphate | |
| MenD (2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC) synthase)variant S32A | Escherichia coli | α-ketoglutarate | 0.23 | 0.0099 | 23 | PMID 21928762 | Open record: MenD (2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC) synthase) variant S32A, α-ketoglutarate | |
| MenD (2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC) synthase)variant S32D | Escherichia coli | isochorismate | 0.042 | 0.26 | 0.158 | PMID 21928762 | Open record: MenD (2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC) synthase) variant S32D, isochorismate | |
| MenD (2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC) synthase)variant S391A | Escherichia coli | (+)-trans-5-(carboxylatomethoxy)-6-hydroxycyclohexa-1,3-diene carboxylate | 0.22 | 0.036 | 6 | PMID 21928762 | Open record: MenD (2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC) synthase) variant S391A, (+)-trans-5-(carboxylatomethoxy)-6-hydroxycyclohexa-1,3-diene carboxylate | |
| MenD (2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC) synthase)variant S391A | Escherichia coli | isochorismate | 0.3 | — | — | PMID 21928762 | Open record: MenD (2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC) synthase) variant S391A, isochorismate | |
| MenD (2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC) synthase)variant S391A | Escherichia coli | thiamine diphosphate | 0.3 | 0.017 | 17 | PMID 21928762 | Open record: MenD (2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC) synthase) variant S391A, thiamine diphosphate | |
| MenD (2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC) synthase)variant S391A | Escherichia coli | α-ketoglutarate | 0.3 | 0.013 | 23 | PMID 21928762 | Open record: MenD (2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC) synthase) variant S391A, α-ketoglutarate | |
| MenD (SEPHCHC synthase) | Escherichia coli | isochorismate | 0.337 | 8 × 10⁻⁵ | 400 | pH 7 | PMID 30341164 | Open record: MenD (SEPHCHC synthase), isochorismate |
| MenD (SEPHCHC synthase) | Escherichia coli | α-ketoglutarate | 0.287 | 0.0029 | 98 | pH 7 | PMID 30341164 | Open record: MenD (SEPHCHC synthase), α-ketoglutarate |
| MenD (SEPHCHC synthase)variant R395A | Escherichia coli | isochorismate | 0.0652 | 0.016 | 4 | pH 7 | PMID 30341164 | Open record: MenD (SEPHCHC synthase) variant R395A, isochorismate |
| MenD (SEPHCHC synthase)variant R395A | Escherichia coli | α-ketoglutarate | 0.0178 | 0.029 | 0.62 | pH 7 | PMID 30341164 | Open record: MenD (SEPHCHC synthase) variant R395A, α-ketoglutarate |
| MenD (SEPHCHC synthase)variant R395K | Escherichia coli | isochorismate | 0.1345 | 0.0022 | 6.2 | pH 7 | PMID 30341164 | Open record: MenD (SEPHCHC synthase) variant R395K, isochorismate |
| MenD (SEPHCHC synthase)variant R395K | Escherichia coli | α-ketoglutarate | 0.1437 | 0.067 | 2.2 | pH 7 | PMID 30341164 | Open record: MenD (SEPHCHC synthase) variant R395K, α-ketoglutarate |
| MenD (SEPHCHC synthase)variant R395K/R413K | Escherichia coli | isochorismate | 0.0025 | 8.2 × 10⁻⁴ | 0.3 | pH 7 | PMID 30341164 | Open record: MenD (SEPHCHC synthase) variant R395K/R413K, isochorismate |
| MenD (SEPHCHC synthase)variant R395K/R413K | Escherichia coli | α-ketoglutarate | 0.0027 | 0.172 | 0.0155 | pH 7 | PMID 30341164 | Open record: MenD (SEPHCHC synthase) variant R395K/R413K, α-ketoglutarate |
| MenD (SEPHCHC synthase)variant R413K | Escherichia coli | isochorismate | 0.0383 | 3.5 × 10⁻⁴ | 110 | pH 7 | PMID 30341164 | Open record: MenD (SEPHCHC synthase) variant R413K, isochorismate |
| MenD (SEPHCHC synthase)variant R413K | Escherichia coli | α-ketoglutarate | 0.0428 | 0.115 | 0.37 | pH 7 | PMID 30341164 | Open record: MenD (SEPHCHC synthase) variant R413K, α-ketoglutarate |