Values CatLog read from this paper
Using substrate analogues to probe the kinetic mechanism and active site of Escherichia coli MenD
PMID 21928762doi:10.1021/bi201202nOpen in Browse
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CatLog read 45 records (120 values) from this paper: MenD (2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC) synthase) from Escherichia coli.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| MenD (2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC) synthase) | Escherichia coli | (+)-trans-5-(carboxylatomethoxy)-6-hydroxycyclohexa-1,3-diene carboxylate | 0.45 | 0.0091 | 50 | pH 7.4 | Open record: MenD (2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC) synthase), (+)-trans-5-(carboxylatomethoxy)-6-hydroxycyclohexa-1,3-diene carboxylate |
| MenD (2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC) synthase) | Escherichia coli | (+)-trans-5-(carboxylatomethoxy)-6-hydroxycyclohexa-1,3-diene carboxylate | 0.4 | 0.012 | 33 | Open record: MenD (2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC) synthase), (+)-trans-5-(carboxylatomethoxy)-6-hydroxycyclohexa-1,3-diene carboxylate | |
| MenD (2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC) synthase) | Escherichia coli | isochorismate | 0.28 | — | — | Open record: MenD (2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC) synthase), isochorismate | |
| MenD (2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC) synthase) | Escherichia coli | thiamine diphosphate | 0.28 | 0.008 | 35 | Open record: MenD (2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC) synthase), thiamine diphosphate | |
| MenD (2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC) synthase) | Escherichia coli | α-ketoglutarate | — | 0.0038 | 120 | pH 7.4 | Open record: MenD (2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC) synthase), α-ketoglutarate |
| MenD (2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC) synthase) | Escherichia coli | α-ketoglutarate | 0.28 | 0.0099 | 28 | Open record: MenD (2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC) synthase), α-ketoglutarate | |
| MenD (2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC) synthase)variant E55D | Escherichia coli | thiamine diphosphate | 0.22 | 0.024 | 8.8 | Open record: MenD (2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC) synthase) variant E55D, thiamine diphosphate | |
| MenD (2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC) synthase)variant E55D | Escherichia coli | α-ketoglutarate | 0.22 | 0.012 | 18 | Open record: MenD (2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC) synthase) variant E55D, α-ketoglutarate | |
| MenD (2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC) synthase)variant I418L | Escherichia coli | (+)-trans-5-(carboxylatomethoxy)-6-hydroxycyclohexa-1,3-diene carboxylate | 0.38 | 0.027 | 14.2 | Open record: MenD (2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC) synthase) variant I418L, (+)-trans-5-(carboxylatomethoxy)-6-hydroxycyclohexa-1,3-diene carboxylate | |
| MenD (2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC) synthase)variant I418L | Escherichia coli | isochorismate | 0.22 | — | — | Open record: MenD (2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC) synthase) variant I418L, isochorismate | |
| MenD (2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC) synthase)variant I418L | Escherichia coli | α-ketoglutarate | 0.22 | 0.2 | 1.05 | Open record: MenD (2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC) synthase) variant I418L, α-ketoglutarate | |
| MenD (2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC) synthase)variant K292Q | Escherichia coli | (+)-trans-5-(carboxylatomethoxy)-6-hydroxycyclohexa-1,3-diene carboxylate | 0.28 | 0.05 | 5.7 | Open record: MenD (2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC) synthase) variant K292Q, (+)-trans-5-(carboxylatomethoxy)-6-hydroxycyclohexa-1,3-diene carboxylate | |
| MenD (2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC) synthase)variant K292Q | Escherichia coli | isochorismate | 0.2 | — | — | Open record: MenD (2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC) synthase) variant K292Q, isochorismate | |
| MenD (2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC) synthase)variant K292Q | Escherichia coli | thiamine diphosphate | 0.2 | 0.002 | 100 | Open record: MenD (2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC) synthase) variant K292Q, thiamine diphosphate | |
| MenD (2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC) synthase)variant K292Q | Escherichia coli | α-ketoglutarate | 0.2 | 0.0026 | 77 | Open record: MenD (2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC) synthase) variant K292Q, α-ketoglutarate | |
| MenD (2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC) synthase)variant R107K | Escherichia coli | (+)-trans-5-(carboxylatomethoxy)-6-hydroxycyclohexa-1,3-diene carboxylate | 0.118 | 0.091 | 1.3 | Open record: MenD (2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC) synthase) variant R107K, (+)-trans-5-(carboxylatomethoxy)-6-hydroxycyclohexa-1,3-diene carboxylate | |
| MenD (2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC) synthase)variant R107K | Escherichia coli | isochorismate | 0.17 | 0.0075 | 22 | Open record: MenD (2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC) synthase) variant R107K, isochorismate | |
| MenD (2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC) synthase)variant R107K | Escherichia coli | thiamine diphosphate | 0.17 | 0.0048 | 37 | Open record: MenD (2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC) synthase) variant R107K, thiamine diphosphate | |
| MenD (2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC) synthase)variant R107K | Escherichia coli | α-ketoglutarate | 0.17 | 0.0053 | 32 | Open record: MenD (2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC) synthase) variant R107K, α-ketoglutarate | |
| MenD (2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC) synthase)variant R293K | Escherichia coli | (+)-trans-5-(carboxylatomethoxy)-6-hydroxycyclohexa-1,3-diene carboxylate | 0.4 | 0.0042 | 95 | Open record: MenD (2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC) synthase) variant R293K, (+)-trans-5-(carboxylatomethoxy)-6-hydroxycyclohexa-1,3-diene carboxylate | |
| MenD (2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC) synthase)variant R293K | Escherichia coli | isochorismate | 0.3 | — | — | Open record: MenD (2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC) synthase) variant R293K, isochorismate | |
| MenD (2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC) synthase)variant R293K | Escherichia coli | thiamine diphosphate | 0.3 | 0.0047 | 63 | Open record: MenD (2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC) synthase) variant R293K, thiamine diphosphate | |
| MenD (2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC) synthase)variant R293K | Escherichia coli | α-ketoglutarate | 0.3 | 0.0091 | 33 | Open record: MenD (2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC) synthase) variant R293K, α-ketoglutarate | |
| MenD (2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC) synthase)variant R33K | Escherichia coli | isochorismate | 0.105 | 0.14 | 0.78 | Open record: MenD (2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC) synthase) variant R33K, isochorismate | |
| MenD (2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC) synthase)variant R33Q | Escherichia coli | (+)-trans-5-(carboxylatomethoxy)-6-hydroxycyclohexa-1,3-diene carboxylate | 0.04 | 0.43 | 0.093 | Open record: MenD (2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC) synthase) variant R33Q, (+)-trans-5-(carboxylatomethoxy)-6-hydroxycyclohexa-1,3-diene carboxylate | |
| MenD (2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC) synthase)variant R33Q | Escherichia coli | isochorismate | 0.122 | 0.054 | 2.3 | Open record: MenD (2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC) synthase) variant R33Q, isochorismate | |
| MenD (2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC) synthase)variant R395A | Escherichia coli | (+)-trans-5-(carboxylatomethoxy)-6-hydroxycyclohexa-1,3-diene carboxylate | 0.04 | 0.39 | 0.102 | Open record: MenD (2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC) synthase) variant R395A, (+)-trans-5-(carboxylatomethoxy)-6-hydroxycyclohexa-1,3-diene carboxylate | |
| MenD (2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC) synthase)variant R395A | Escherichia coli | isochorismate | 0.043 | 0.036 | 1.2 | Open record: MenD (2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC) synthase) variant R395A, isochorismate | |
| MenD (2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC) synthase)variant R395K | Escherichia coli | (+)-trans-5-(carboxylatomethoxy)-6-hydroxycyclohexa-1,3-diene carboxylate | 0.18 | 0.109 | 1.7 | Open record: MenD (2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC) synthase) variant R395K, (+)-trans-5-(carboxylatomethoxy)-6-hydroxycyclohexa-1,3-diene carboxylate | |
| MenD (2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC) synthase)variant R395K | Escherichia coli | isochorismate | 0.132 | 0.0053 | 25 | Open record: MenD (2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC) synthase) variant R395K, isochorismate | |
| MenD (2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC) synthase)variant R395K | Escherichia coli | thiamine diphosphate | 0.132 | 0.0022 | 60 | Open record: MenD (2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC) synthase) variant R395K, thiamine diphosphate | |
| MenD (2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC) synthase)variant R395K | Escherichia coli | α-ketoglutarate | 0.132 | 0.045 | 3 | Open record: MenD (2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC) synthase) variant R395K, α-ketoglutarate | |
| MenD (2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC) synthase)variant R413K | Escherichia coli | (+)-trans-5-(carboxylatomethoxy)-6-hydroxycyclohexa-1,3-diene carboxylate | 0.17 | 0.01 | 17 | Open record: MenD (2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC) synthase) variant R413K, (+)-trans-5-(carboxylatomethoxy)-6-hydroxycyclohexa-1,3-diene carboxylate | |
| MenD (2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC) synthase)variant R413K | Escherichia coli | isochorismate | 0.22 | — | — | Open record: MenD (2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC) synthase) variant R413K, isochorismate | |
| MenD (2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC) synthase)variant R413K | Escherichia coli | thiamine diphosphate | 0.102 | 0.005 | 20 | Open record: MenD (2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC) synthase) variant R413K, thiamine diphosphate | |
| MenD (2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC) synthase)variant R413K | Escherichia coli | α-ketoglutarate | 0.102 | 0.22 | 0.47 | Open record: MenD (2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC) synthase) variant R413K, α-ketoglutarate | |
| MenD (2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC) synthase)variant S32A | Escherichia coli | (+)-trans-5-(carboxylatomethoxy)-6-hydroxycyclohexa-1,3-diene carboxylate | 0.25 | 0.042 | 5.8 | Open record: MenD (2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC) synthase) variant S32A, (+)-trans-5-(carboxylatomethoxy)-6-hydroxycyclohexa-1,3-diene carboxylate | |
| MenD (2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC) synthase)variant S32A | Escherichia coli | isochorismate | 0.23 | — | — | Open record: MenD (2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC) synthase) variant S32A, isochorismate | |
| MenD (2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC) synthase)variant S32A | Escherichia coli | thiamine diphosphate | 0.23 | 0.0043 | 53 | Open record: MenD (2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC) synthase) variant S32A, thiamine diphosphate | |
| MenD (2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC) synthase)variant S32A | Escherichia coli | α-ketoglutarate | 0.23 | 0.0099 | 23 | Open record: MenD (2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC) synthase) variant S32A, α-ketoglutarate | |
| MenD (2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC) synthase)variant S32D | Escherichia coli | isochorismate | 0.042 | 0.26 | 0.158 | Open record: MenD (2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC) synthase) variant S32D, isochorismate | |
| MenD (2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC) synthase)variant S391A | Escherichia coli | (+)-trans-5-(carboxylatomethoxy)-6-hydroxycyclohexa-1,3-diene carboxylate | 0.22 | 0.036 | 6 | Open record: MenD (2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC) synthase) variant S391A, (+)-trans-5-(carboxylatomethoxy)-6-hydroxycyclohexa-1,3-diene carboxylate | |
| MenD (2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC) synthase)variant S391A | Escherichia coli | isochorismate | 0.3 | — | — | Open record: MenD (2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC) synthase) variant S391A, isochorismate | |
| MenD (2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC) synthase)variant S391A | Escherichia coli | thiamine diphosphate | 0.3 | 0.017 | 17 | Open record: MenD (2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC) synthase) variant S391A, thiamine diphosphate | |
| MenD (2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC) synthase)variant S391A | Escherichia coli | α-ketoglutarate | 0.3 | 0.013 | 23 | Open record: MenD (2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC) synthase) variant S391A, α-ketoglutarate |