Values CatLog read from this paper
Two active site arginines are critical determinants of substrate binding and catalysis in MenD: a thiamine-dependent enzyme in menaquinone biosynthesis
PMID 30341164doi:10.1042/bcj20180548Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 10 records (30 values) from this paper: MenD (SEPHCHC synthase) from Escherichia coli.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| MenD (SEPHCHC synthase) | Escherichia coli | isochorismate | 0.337 | 8 × 10⁻⁵ | 400 | pH 7 | Open record: MenD (SEPHCHC synthase), isochorismate |
| MenD (SEPHCHC synthase) | Escherichia coli | α-ketoglutarate | 0.287 | 0.0029 | 98 | pH 7 | Open record: MenD (SEPHCHC synthase), α-ketoglutarate |
| MenD (SEPHCHC synthase)variant R395A | Escherichia coli | isochorismate | 0.0652 | 0.016 | 4 | pH 7 | Open record: MenD (SEPHCHC synthase) variant R395A, isochorismate |
| MenD (SEPHCHC synthase)variant R395A | Escherichia coli | α-ketoglutarate | 0.0178 | 0.029 | 0.62 | pH 7 | Open record: MenD (SEPHCHC synthase) variant R395A, α-ketoglutarate |
| MenD (SEPHCHC synthase)variant R395K | Escherichia coli | isochorismate | 0.1345 | 0.0022 | 6.2 | pH 7 | Open record: MenD (SEPHCHC synthase) variant R395K, isochorismate |
| MenD (SEPHCHC synthase)variant R395K | Escherichia coli | α-ketoglutarate | 0.1437 | 0.067 | 2.2 | pH 7 | Open record: MenD (SEPHCHC synthase) variant R395K, α-ketoglutarate |
| MenD (SEPHCHC synthase)variant R395K/R413K | Escherichia coli | isochorismate | 0.0025 | 8.2 × 10⁻⁴ | 0.3 | pH 7 | Open record: MenD (SEPHCHC synthase) variant R395K/R413K, isochorismate |
| MenD (SEPHCHC synthase)variant R395K/R413K | Escherichia coli | α-ketoglutarate | 0.0027 | 0.172 | 0.0155 | pH 7 | Open record: MenD (SEPHCHC synthase) variant R395K/R413K, α-ketoglutarate |
| MenD (SEPHCHC synthase)variant R413K | Escherichia coli | isochorismate | 0.0383 | 3.5 × 10⁻⁴ | 110 | pH 7 | Open record: MenD (SEPHCHC synthase) variant R413K, isochorismate |
| MenD (SEPHCHC synthase)variant R413K | Escherichia coli | α-ketoglutarate | 0.0428 | 0.115 | 0.37 | pH 7 | Open record: MenD (SEPHCHC synthase) variant R413K, α-ketoglutarate |