Values CatLog read from this paper
Asparagine362 is essential for zinc binding and catalysis in the peptidase reaction of Saccharomyces cerevisiae leukotriene A₄ hydrolase
PMID 20624488doi:10.1016/j.bbapap.2010.07.005Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 32 records (64 values) from this paper: leukotriene A4 hydrolase (aminopeptidase activity) from Saccharomyces cerevisiae.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 32 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| leukotriene A4 hydrolase (aminopeptidase activity) | Saccharomyces cerevisiae | alanine β-naphthylamide | 0.027 | 1.78 | 0.015 calc. | 25 °C | Open record: leukotriene A4 hydrolase (aminopeptidase activity), alanine β-naphthylamide |
| leukotriene A4 hydrolase (aminopeptidase activity) | Saccharomyces cerevisiae | arginine β-naphthylamide | 0.0152 | 0.366 | 0.0410 calc. | 25 °C | Open record: leukotriene A4 hydrolase (aminopeptidase activity), arginine β-naphthylamide |
| leukotriene A4 hydrolase (aminopeptidase activity) | Saccharomyces cerevisiae | isoleucine β-naphthylamide | 0.0145 | 0.566 | 0.0260 calc. | 25 °C | Open record: leukotriene A4 hydrolase (aminopeptidase activity), isoleucine β-naphthylamide |
| leukotriene A4 hydrolase (aminopeptidase activity) | Saccharomyces cerevisiae | leucine β-naphthylamide | 0.147 | 0.745 | 0.200 calc. | 25 °C | Open record: leukotriene A4 hydrolase (aminopeptidase activity), leucine β-naphthylamide |
| leukotriene A4 hydrolase (aminopeptidase activity) | Saccharomyces cerevisiae | methionine β-naphthylamide | 0.147 | 0.7 | 0.21 calc. | 25 °C | Open record: leukotriene A4 hydrolase (aminopeptidase activity), methionine β-naphthylamide |
| leukotriene A4 hydrolase (aminopeptidase activity) | Saccharomyces cerevisiae | valine β-naphthylamide | 0.0193 | 0.384 | 0.0503 calc. | 25 °C | Open record: leukotriene A4 hydrolase (aminopeptidase activity), valine β-naphthylamide |
| leukotriene A4 hydrolase (aminopeptidase activity)variant D399A | Saccharomyces cerevisiae | alanine β-naphthylamide | 0.065 | 0.885 | 0.073 calc. | 25 °C | Open record: leukotriene A4 hydrolase (aminopeptidase activity) variant D399A, alanine β-naphthylamide |
| leukotriene A4 hydrolase (aminopeptidase activity)variant D399A | Saccharomyces cerevisiae | arginine β-naphthylamide | 0.023 | 0.199 | 0.12 calc. | 25 °C | Open record: leukotriene A4 hydrolase (aminopeptidase activity) variant D399A, arginine β-naphthylamide |
| leukotriene A4 hydrolase (aminopeptidase activity)variant D399A | Saccharomyces cerevisiae | isoleucine β-naphthylamide | 0.0168 | 0.657 | 0.0256 calc. | 25 °C | Open record: leukotriene A4 hydrolase (aminopeptidase activity) variant D399A, isoleucine β-naphthylamide |
| leukotriene A4 hydrolase (aminopeptidase activity)variant D399A | Saccharomyces cerevisiae | leucine β-naphthylamide | 0.165 | 0.693 | 0.240 calc. | 25 °C | Open record: leukotriene A4 hydrolase (aminopeptidase activity) variant D399A, leucine β-naphthylamide |
| leukotriene A4 hydrolase (aminopeptidase activity)variant D399A | Saccharomyces cerevisiae | methionine β-naphthylamide | 0.098 | 0.374 | 0.26 calc. | 25 °C | Open record: leukotriene A4 hydrolase (aminopeptidase activity) variant D399A, methionine β-naphthylamide |
| leukotriene A4 hydrolase (aminopeptidase activity)variant D399A | Saccharomyces cerevisiae | valine β-naphthylamide | 0.0242 | 0.803 | 0.0301 calc. | 25 °C | Open record: leukotriene A4 hydrolase (aminopeptidase activity) variant D399A, valine β-naphthylamide |
| leukotriene A4 hydrolase (aminopeptidase activity)variant N362E | Saccharomyces cerevisiae | alanine β-naphthylamide | 0.003 | 0.618 | 0.0049 calc. | 25 °C | Open record: leukotriene A4 hydrolase (aminopeptidase activity) variant N362E, alanine β-naphthylamide |
| leukotriene A4 hydrolase (aminopeptidase activity)variant N362E | Saccharomyces cerevisiae | arginine β-naphthylamide | 0.005 | 0.587 | 0.0085 calc. | 25 °C | Open record: leukotriene A4 hydrolase (aminopeptidase activity) variant N362E, arginine β-naphthylamide |
| leukotriene A4 hydrolase (aminopeptidase activity)variant N362E | Saccharomyces cerevisiae | leucine β-naphthylamide | 0.0065 | 0.365 | 0.018 calc. | 25 °C | Open record: leukotriene A4 hydrolase (aminopeptidase activity) variant N362E, leucine β-naphthylamide |
| leukotriene A4 hydrolase (aminopeptidase activity)variant N362E | Saccharomyces cerevisiae | methionine β-naphthylamide | 0.0087 | 0.166 | 0.052 calc. | 25 °C | Open record: leukotriene A4 hydrolase (aminopeptidase activity) variant N362E, methionine β-naphthylamide |
| leukotriene A4 hydrolase (aminopeptidase activity)variant N362L | Saccharomyces cerevisiae | alanine β-naphthylamide | 4.7 × 10⁻⁴ | 0.31 | 0.0015 calc. | 25 °C | Open record: leukotriene A4 hydrolase (aminopeptidase activity) variant N362L, alanine β-naphthylamide |
| leukotriene A4 hydrolase (aminopeptidase activity)variant N362L | Saccharomyces cerevisiae | arginine β-naphthylamide | 2.8 × 10⁻⁴ | 0.49 | 5.8 × 10⁻⁴ calc. | 25 °C | Open record: leukotriene A4 hydrolase (aminopeptidase activity) variant N362L, arginine β-naphthylamide |
| leukotriene A4 hydrolase (aminopeptidase activity)variant N362L | Saccharomyces cerevisiae | leucine β-naphthylamide | 0.00135 | 0.29 | 0.0047 calc. | 25 °C | Open record: leukotriene A4 hydrolase (aminopeptidase activity) variant N362L, leucine β-naphthylamide |
| leukotriene A4 hydrolase (aminopeptidase activity)variant N362L | Saccharomyces cerevisiae | methionine β-naphthylamide | 0.0032 | 0.128 | 0.025 calc. | 25 °C | Open record: leukotriene A4 hydrolase (aminopeptidase activity) variant N362L, methionine β-naphthylamide |
| leukotriene A4 hydrolase (aminopeptidase activity)variant N362Q | Saccharomyces cerevisiae | alanine β-naphthylamide | 0.0128 | 0.4 | 0.032 calc. | 25 °C | Open record: leukotriene A4 hydrolase (aminopeptidase activity) variant N362Q, alanine β-naphthylamide |
| leukotriene A4 hydrolase (aminopeptidase activity)variant N362Q | Saccharomyces cerevisiae | arginine β-naphthylamide | 0.0123 | 0.137 | 0.0900 calc. | 25 °C | Open record: leukotriene A4 hydrolase (aminopeptidase activity) variant N362Q, arginine β-naphthylamide |
| leukotriene A4 hydrolase (aminopeptidase activity)variant N362Q | Saccharomyces cerevisiae | isoleucine β-naphthylamide | 0.0085 | 0.698 | 0.012 calc. | 25 °C | Open record: leukotriene A4 hydrolase (aminopeptidase activity) variant N362Q, isoleucine β-naphthylamide |
| leukotriene A4 hydrolase (aminopeptidase activity)variant N362Q | Saccharomyces cerevisiae | leucine β-naphthylamide | 0.006 | 0.105 | 0.057 calc. | 25 °C | Open record: leukotriene A4 hydrolase (aminopeptidase activity) variant N362Q, leucine β-naphthylamide |
| leukotriene A4 hydrolase (aminopeptidase activity)variant N362Q | Saccharomyces cerevisiae | methionine β-naphthylamide | 0.0265 | 0.15 | 0.18 calc. | 25 °C | Open record: leukotriene A4 hydrolase (aminopeptidase activity) variant N362Q, methionine β-naphthylamide |
| leukotriene A4 hydrolase (aminopeptidase activity)variant N362Q | Saccharomyces cerevisiae | valine β-naphthylamide | 0.0037 | 0.235 | 0.016 calc. | 25 °C | Open record: leukotriene A4 hydrolase (aminopeptidase activity) variant N362Q, valine β-naphthylamide |
| leukotriene A4 hydrolase (aminopeptidase activity)variant W365L | Saccharomyces cerevisiae | alanine β-naphthylamide | 0.1088 | 0.339 | 0.321 calc. | 25 °C | Open record: leukotriene A4 hydrolase (aminopeptidase activity) variant W365L, alanine β-naphthylamide |
| leukotriene A4 hydrolase (aminopeptidase activity)variant W365L | Saccharomyces cerevisiae | arginine β-naphthylamide | 0.0388 | 0.275 | 0.141 calc. | 25 °C | Open record: leukotriene A4 hydrolase (aminopeptidase activity) variant W365L, arginine β-naphthylamide |
| leukotriene A4 hydrolase (aminopeptidase activity)variant W365L | Saccharomyces cerevisiae | isoleucine β-naphthylamide | 0.0268 | 0.488 | 0.0550 calc. | 25 °C | Open record: leukotriene A4 hydrolase (aminopeptidase activity) variant W365L, isoleucine β-naphthylamide |
| leukotriene A4 hydrolase (aminopeptidase activity)variant W365L | Saccharomyces cerevisiae | leucine β-naphthylamide | 0.225 | 0.771 | 0.292 calc. | 25 °C | Open record: leukotriene A4 hydrolase (aminopeptidase activity) variant W365L, leucine β-naphthylamide |
| leukotriene A4 hydrolase (aminopeptidase activity)variant W365L | Saccharomyces cerevisiae | methionine β-naphthylamide | 0.1588 | 0.351 | 0.453 calc. | 25 °C | Open record: leukotriene A4 hydrolase (aminopeptidase activity) variant W365L, methionine β-naphthylamide |
| leukotriene A4 hydrolase (aminopeptidase activity)variant W365L | Saccharomyces cerevisiae | valine β-naphthylamide | 0.0343 | 0.808 | 0.0425 calc. | 25 °C | Open record: leukotriene A4 hydrolase (aminopeptidase activity) variant W365L, valine β-naphthylamide |