Values CatLog read from this paper

Investigating the coenzyme specificity of phenylacetone monooxygenase from Thermobifida fusca

PMID 20703875doi:10.1007/s00253-010-2769-yOpen in Browse

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CatLog read 32 records (96 values) from this paper: phenylacetone monooxygenase from Thermobifida fusca.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.

EC 1.14.13.92

Records read from this paper
Enzyme	Organism	Substrate	k_cat (s⁻¹)	K_m (mM)	k_cat/K_m (mM⁻¹ s⁻¹)	Conditions	Open record
phenylacetone monooxygenase	Thermobifida fusca	NADH	0.39	0.56	0.7	pH 7.5, 25 °C	Open record: phenylacetone monooxygenase, NADH
phenylacetone monooxygenase	Thermobifida fusca	NADPH	3.1	7 × 10⁻⁴	4 × 10³	pH 7.5, 25 °C	Open record: phenylacetone monooxygenase, NADPH
phenylacetone monooxygenasevariant H220A	Thermobifida fusca	NADH	0.51	0.51	1	pH 7.5, 25 °C	Open record: phenylacetone monooxygenase variant H220A, NADH
phenylacetone monooxygenasevariant H220A	Thermobifida fusca	NADPH	3.3	7 × 10⁻⁴	5 × 10³	pH 7.5, 25 °C	Open record: phenylacetone monooxygenase variant H220A, NADPH
phenylacetone monooxygenasevariant H220D	Thermobifida fusca	NADH	0.76	0.78	0.97	pH 7.5, 25 °C	Open record: phenylacetone monooxygenase variant H220D, NADH
phenylacetone monooxygenasevariant H220D	Thermobifida fusca	NADPH	1.2	0.036	33	pH 7.5, 25 °C	Open record: phenylacetone monooxygenase variant H220D, NADPH
phenylacetone monooxygenasevariant H220E	Thermobifida fusca	NADH	0.28	0.71	0.39	pH 7.5, 25 °C	Open record: phenylacetone monooxygenase variant H220E, NADH
phenylacetone monooxygenasevariant H220E	Thermobifida fusca	NADPH	1.2	0.17	7	pH 7.5, 25 °C	Open record: phenylacetone monooxygenase variant H220E, NADPH
phenylacetone monooxygenasevariant H220F	Thermobifida fusca	NADH	0.25	0.75	0.33	pH 7.5, 25 °C	Open record: phenylacetone monooxygenase variant H220F, NADH
phenylacetone monooxygenasevariant H220F	Thermobifida fusca	NADPH	1.9	0.02	95	pH 7.5, 25 °C	Open record: phenylacetone monooxygenase variant H220F, NADPH
phenylacetone monooxygenasevariant H220N	Thermobifida fusca	NADH	0.59	0.26	2.3	pH 7.5, 25 °C	Open record: phenylacetone monooxygenase variant H220N, NADH
phenylacetone monooxygenasevariant H220N	Thermobifida fusca	NADPH	3.6	6 × 10⁻⁴	6 × 10³	pH 7.5, 25 °C	Open record: phenylacetone monooxygenase variant H220N, NADPH
phenylacetone monooxygenasevariant H220Q	Thermobifida fusca	NADH	0.35	0.15	2.3	pH 7.5, 25 °C	Open record: phenylacetone monooxygenase variant H220Q, NADH
phenylacetone monooxygenasevariant H220Q	Thermobifida fusca	NADPH	2.7	0.0017	1.6 × 10³	pH 7.5, 25 °C	Open record: phenylacetone monooxygenase variant H220Q, NADPH
phenylacetone monooxygenasevariant H220Q/K336H	Thermobifida fusca	NADH	0.67	2.4	0.28	pH 7.5, 25 °C	Open record: phenylacetone monooxygenase variant H220Q/K336H, NADH
phenylacetone monooxygenasevariant H220Q/K336H	Thermobifida fusca	NADPH	1.9	0.011	170	pH 7.5, 25 °C	Open record: phenylacetone monooxygenase variant H220Q/K336H, NADPH
phenylacetone monooxygenasevariant H220Q/K336N	Thermobifida fusca	NADH	0.36	0.81	0.44	pH 7.5, 25 °C	Open record: phenylacetone monooxygenase variant H220Q/K336N, NADH
phenylacetone monooxygenasevariant H220Q/K336N	Thermobifida fusca	NADPH	1	0.0024	420	pH 7.5, 25 °C	Open record: phenylacetone monooxygenase variant H220Q/K336N, NADPH
phenylacetone monooxygenasevariant H220T	Thermobifida fusca	NADH	0.43	0.8	0.54	pH 7.5, 25 °C	Open record: phenylacetone monooxygenase variant H220T, NADH
phenylacetone monooxygenasevariant H220T	Thermobifida fusca	NADPH	2.9	0.0017	1.7 × 10³	pH 7.5, 25 °C	Open record: phenylacetone monooxygenase variant H220T, NADPH
phenylacetone monooxygenasevariant H220W	Thermobifida fusca	NADH	0.19	0.86	0.22	pH 7.5, 25 °C	Open record: phenylacetone monooxygenase variant H220W, NADH
phenylacetone monooxygenasevariant H220W	Thermobifida fusca	NADPH	0.8	0.0023	300	pH 7.5, 25 °C	Open record: phenylacetone monooxygenase variant H220W, NADPH
phenylacetone monooxygenasevariant K336H	Thermobifida fusca	NADH	0.22	1.1	0.2	pH 7.5, 25 °C	Open record: phenylacetone monooxygenase variant K336H, NADH
phenylacetone monooxygenasevariant K336H	Thermobifida fusca	NADPH	1.2	0.011	110	pH 7.5, 25 °C	Open record: phenylacetone monooxygenase variant K336H, NADPH
phenylacetone monooxygenasevariant K336N	Thermobifida fusca	NADH	0.39	0.91	0.43	pH 7.5, 25 °C	Open record: phenylacetone monooxygenase variant K336N, NADH
phenylacetone monooxygenasevariant K336N	Thermobifida fusca	NADPH	1.6	0.0011	1.5 × 10³	pH 7.5, 25 °C	Open record: phenylacetone monooxygenase variant K336N, NADPH
phenylacetone monooxygenasevariant R217A	Thermobifida fusca	NADH	0.25	1.9	0.13	pH 7.5, 25 °C	Open record: phenylacetone monooxygenase variant R217A, NADH
phenylacetone monooxygenasevariant R217A	Thermobifida fusca	NADPH	2.2	0.32	7	pH 7.5, 25 °C	Open record: phenylacetone monooxygenase variant R217A, NADPH
phenylacetone monooxygenasevariant R217L	Thermobifida fusca	NADH	0.22	1.44	0.15	pH 7.5, 25 °C	Open record: phenylacetone monooxygenase variant R217L, NADH
phenylacetone monooxygenasevariant R217L	Thermobifida fusca	NADPH	0.8	0.85	1	pH 7.5, 25 °C	Open record: phenylacetone monooxygenase variant R217L, NADPH
phenylacetone monooxygenasevariant T218A	Thermobifida fusca	NADH	0.76	1.11	0.68	pH 7.5, 25 °C	Open record: phenylacetone monooxygenase variant T218A, NADH
phenylacetone monooxygenasevariant T218A	Thermobifida fusca	NADPH	3.8	0.0014	2.7 × 10³	pH 7.5, 25 °C	Open record: phenylacetone monooxygenase variant T218A, NADPH