Values CatLog read from this paper
Insights into the mechanism of dihydropyrimidine dehydrogenase from site-directed mutagenesis targeting the active site loop and redox cofactor coordination
PMID 20831907doi:10.1016/j.bbapap.2010.08.014Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 11 records (26 values) from this paper: dihydropyrimidine dehydrogenase (DPD) from Sus scrofa (pig).
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| dihydropyrimidine dehydrogenase (DPD) | Sus scrofa (pig) | NADPH | — | 0.0057 | 42 | pH 7.3, 30 °C | Open record: dihydropyrimidine dehydrogenase (DPD), NADPH |
| dihydropyrimidine dehydrogenase (DPD) | Sus scrofa (pig) | uracil | 0.242 | 0.0012 | 208 | pH 7.3, 30 °C | Open record: dihydropyrimidine dehydrogenase (DPD), uracil |
| dihydropyrimidine dehydrogenase (DPD)variant C126A | Sus scrofa (pig) | NADPH | — | 0.0061 | 42 | pH 7.3, 30 °C | Open record: dihydropyrimidine dehydrogenase (DPD) variant C126A, NADPH |
| dihydropyrimidine dehydrogenase (DPD)variant C126A | Sus scrofa (pig) | uracil | 0.256 | 0.0011 | 238 | pH 7.3, 30 °C | Open record: dihydropyrimidine dehydrogenase (DPD) variant C126A, uracil |
| dihydropyrimidine dehydrogenase (DPD)variant H673N | Sus scrofa (pig) | NADPH | — | 0.0075 | 9 | pH 7.3, 30 °C | Open record: dihydropyrimidine dehydrogenase (DPD) variant H673N, NADPH |
| dihydropyrimidine dehydrogenase (DPD)variant H673N | Sus scrofa (pig) | uracil | 0.068 | 0.007 | 10 | pH 7.3, 30 °C | Open record: dihydropyrimidine dehydrogenase (DPD) variant H673N, uracil |
| dihydropyrimidine dehydrogenase (DPD)variant H673Q | Sus scrofa (pig) | NADPH | — | 0.0062 | 19 | pH 7.3, 30 °C | Open record: dihydropyrimidine dehydrogenase (DPD) variant H673Q, NADPH |
| dihydropyrimidine dehydrogenase (DPD)variant H673Q | Sus scrofa (pig) | uracil | 0.117 | 0.0042 | 28 | pH 7.3, 30 °C | Open record: dihydropyrimidine dehydrogenase (DPD) variant H673Q, uracil |
| dihydropyrimidine dehydrogenase (DPD)variant S670A | Sus scrofa (pig) | NADPHparameters from ping-pong fit (Eq. 3); cosubstrate uracil | — | 0.0188 | 9 | pH 7.3, 30 °C | Open record: dihydropyrimidine dehydrogenase (DPD) variant S670A, NADPH |
| dihydropyrimidine dehydrogenase (DPD)variant S670A | Sus scrofa (pig) | NADPH | — | 0.046 | — | pH 7.3, 30 °C | Open record: dihydropyrimidine dehydrogenase (DPD) variant S670A, NADPH |
| dihydropyrimidine dehydrogenase (DPD)variant S670A | Sus scrofa (pig) | uracil | 0.177 | 0.0366 | 5 | pH 7.3, 30 °C | Open record: dihydropyrimidine dehydrogenase (DPD) variant S670A, uracil |