EC 1.3.1.2: dihydropyrimidine dehydrogenase (NADP+)
OxidoreductasesOpen in Browse
CatLog read 23 records for EC 1.3.1.2 from 2 papers, for 2 organisms. Names in these papers: dihydropyrimidine dehydrogenase (DPD).
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units.
CatLog also holds 34 records for EC 1.3.1.2 that are not yet re-read: from its earlier version, built from the enzyme databases and partly checked against the papers. All records for EC 1.3.1.2 in Browse.
Papers
| Paper | Organisms | Records |
|---|---|---|
| Transient-State Analysis of Porcine Dihydropyrimidine Dehydrogenase Reveals Reductive Activation by NADPH | Sus scrofa | 12 |
| Insights into the mechanism of dihydropyrimidine dehydrogenase from site-directed mutagenesis targeting the active site loop and redox cofactor coordination | Sus scrofa (pig) | 11 |
Records
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Paper | Open record |
|---|---|---|---|---|---|---|---|---|
| dihydropyrimidine dehydrogenase (DPD) | Sus scrofa | 5-methyluracilaerobic steady state; 2 mM DTT | 0.42 | 0.0046 | 90 | pH 7.4, 20 °C | PMID 32516529 | Open record: dihydropyrimidine dehydrogenase (DPD), 5-methyluracil |
| dihydropyrimidine dehydrogenase (DPD) | Sus scrofa | 5-methyluracilanaerobic steady state by stopped-flow | 0.39 | 7.9 × 10⁻⁴ | 500 | pH 7.4, 20 °C | PMID 32516529 | Open record: dihydropyrimidine dehydrogenase (DPD), 5-methyluracil |
| dihydropyrimidine dehydrogenase (DPD) | Sus scrofa | reduced nicotinamide adenine dinucleotide phosphateaerobic steady state; 2 mM DTT; uracil fixed at 200 µM | 0.65 | 0.0054 | 120 | pH 7.4, 20 °C | PMID 32516529 | Open record: dihydropyrimidine dehydrogenase (DPD), reduced nicotinamide adenine dinucleotide phosphate |
| dihydropyrimidine dehydrogenase (DPD) | Sus scrofa | reduced nicotinamide adenine dinucleotide phosphateanaerobic steady state by stopped-flow; cosubstrate uracil | 0.85 | 0.0023 | 360 | pH 7.4, 20 °C | PMID 32516529 | Open record: dihydropyrimidine dehydrogenase (DPD), reduced nicotinamide adenine dinucleotide phosphate |
| dihydropyrimidine dehydrogenase (DPD) | Sus scrofa | reduced nicotinamide adenine dinucleotide phosphateNADPH varied (0-1 mM); ~250 µM O2 (aerobic); 2 mM DTT | 0.043 | — | — | pH 7.4, 20 °C | PMID 32516529 | Open record: dihydropyrimidine dehydrogenase (DPD), reduced nicotinamide adenine dinucleotide phosphate |
| dihydropyrimidine dehydrogenase (DPD) | Sus scrofa | reduced nicotinamide adenine dinucleotide phosphateNADPH varied (0-1 mM); ~250 µM O2 (aerobic); 2 mM DTT | 0.015 | — | — | pH 7.4, 20 °C | PMID 32516529 | Open record: dihydropyrimidine dehydrogenase (DPD), reduced nicotinamide adenine dinucleotide phosphate |
| dihydropyrimidine dehydrogenase (DPD) | Sus scrofa | uracilaerobic steady state; 2 mM DTT; NADPH fixed at 200 µM | 0.73 | 0.0063 | 110 | pH 7.4, 20 °C | PMID 32516529 | Open record: dihydropyrimidine dehydrogenase (DPD), uracil |
| dihydropyrimidine dehydrogenase (DPD) | Sus scrofa | uracilanaerobic steady state by stopped-flow | 0.78 | 8.7 × 10⁻⁴ | 900 | pH 7.4, 20 °C | PMID 32516529 | Open record: dihydropyrimidine dehydrogenase (DPD), uracil |
| dihydropyrimidine dehydrogenase (DPD)variant C671S | Sus scrofa | 5-methyluracil | 2.4 × 10⁻⁴ | — | — | pH 7.4, 20 °C | PMID 32516529 | Open record: dihydropyrimidine dehydrogenase (DPD) variant C671S, 5-methyluracil |
| dihydropyrimidine dehydrogenase (DPD)variant C671S | Sus scrofa | reduced nicotinamide adenine dinucleotide phosphate | 0.015 | 0.00931 | 2 | pH 7.4, 20 °C | PMID 32516529 | Open record: dihydropyrimidine dehydrogenase (DPD) variant C671S, reduced nicotinamide adenine dinucleotide phosphate |
| dihydropyrimidine dehydrogenase (DPD)variant C671S | Sus scrofa | uracilaerobic steady state; 2 mM DTT; NADPH fixed at 200 µM | 0.03 | 0.00148 | 20 | pH 7.4, 20 °C | PMID 32516529 | Open record: dihydropyrimidine dehydrogenase (DPD) variant C671S, uracil |
| dihydropyrimidine dehydrogenase (DPD)variant C671S | Sus scrofa | uracilanaerobic steady state by stopped-flow | 0.015 | 0.00548 | 3 | pH 7.4, 20 °C | PMID 32516529 | Open record: dihydropyrimidine dehydrogenase (DPD) variant C671S, uracil |
| dihydropyrimidine dehydrogenase (DPD) | Sus scrofa (pig) | NADPH | — | 0.0057 | 42 | pH 7.3, 30 °C | PMID 20831907 | Open record: dihydropyrimidine dehydrogenase (DPD), NADPH |
| dihydropyrimidine dehydrogenase (DPD) | Sus scrofa (pig) | uracil | 0.242 | 0.0012 | 208 | pH 7.3, 30 °C | PMID 20831907 | Open record: dihydropyrimidine dehydrogenase (DPD), uracil |
| dihydropyrimidine dehydrogenase (DPD)variant C126A | Sus scrofa (pig) | NADPH | — | 0.0061 | 42 | pH 7.3, 30 °C | PMID 20831907 | Open record: dihydropyrimidine dehydrogenase (DPD) variant C126A, NADPH |
| dihydropyrimidine dehydrogenase (DPD)variant C126A | Sus scrofa (pig) | uracil | 0.256 | 0.0011 | 238 | pH 7.3, 30 °C | PMID 20831907 | Open record: dihydropyrimidine dehydrogenase (DPD) variant C126A, uracil |
| dihydropyrimidine dehydrogenase (DPD)variant H673N | Sus scrofa (pig) | NADPH | — | 0.0075 | 9 | pH 7.3, 30 °C | PMID 20831907 | Open record: dihydropyrimidine dehydrogenase (DPD) variant H673N, NADPH |
| dihydropyrimidine dehydrogenase (DPD)variant H673N | Sus scrofa (pig) | uracil | 0.068 | 0.007 | 10 | pH 7.3, 30 °C | PMID 20831907 | Open record: dihydropyrimidine dehydrogenase (DPD) variant H673N, uracil |
| dihydropyrimidine dehydrogenase (DPD)variant H673Q | Sus scrofa (pig) | NADPH | — | 0.0062 | 19 | pH 7.3, 30 °C | PMID 20831907 | Open record: dihydropyrimidine dehydrogenase (DPD) variant H673Q, NADPH |
| dihydropyrimidine dehydrogenase (DPD)variant H673Q | Sus scrofa (pig) | uracil | 0.117 | 0.0042 | 28 | pH 7.3, 30 °C | PMID 20831907 | Open record: dihydropyrimidine dehydrogenase (DPD) variant H673Q, uracil |
| dihydropyrimidine dehydrogenase (DPD)variant S670A | Sus scrofa (pig) | NADPHparameters from ping-pong fit (Eq. 3); cosubstrate uracil | — | 0.0188 | 9 | pH 7.3, 30 °C | PMID 20831907 | Open record: dihydropyrimidine dehydrogenase (DPD) variant S670A, NADPH |
| dihydropyrimidine dehydrogenase (DPD)variant S670A | Sus scrofa (pig) | NADPH | — | 0.046 | — | pH 7.3, 30 °C | PMID 20831907 | Open record: dihydropyrimidine dehydrogenase (DPD) variant S670A, NADPH |
| dihydropyrimidine dehydrogenase (DPD)variant S670A | Sus scrofa (pig) | uracil | 0.177 | 0.0366 | 5 | pH 7.3, 30 °C | PMID 20831907 | Open record: dihydropyrimidine dehydrogenase (DPD) variant S670A, uracil |