Values CatLog read from this paper
Transient-State Analysis of Porcine Dihydropyrimidine Dehydrogenase Reveals Reductive Activation by NADPH
PMID 32516529doi:10.1021/acs.biochem.0c00223Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 12 records (30 values) from this paper: dihydropyrimidine dehydrogenase (DPD) from Sus scrofa.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| dihydropyrimidine dehydrogenase (DPD) | Sus scrofa | 5-methyluracilaerobic steady state; 2 mM DTT | 0.42 | 0.0046 | 90 | pH 7.4, 20 °C | Open record: dihydropyrimidine dehydrogenase (DPD), 5-methyluracil |
| dihydropyrimidine dehydrogenase (DPD) | Sus scrofa | 5-methyluracilanaerobic steady state by stopped-flow | 0.39 | 7.9 × 10⁻⁴ | 500 | pH 7.4, 20 °C | Open record: dihydropyrimidine dehydrogenase (DPD), 5-methyluracil |
| dihydropyrimidine dehydrogenase (DPD) | Sus scrofa | reduced nicotinamide adenine dinucleotide phosphateaerobic steady state; 2 mM DTT; uracil fixed at 200 µM | 0.65 | 0.0054 | 120 | pH 7.4, 20 °C | Open record: dihydropyrimidine dehydrogenase (DPD), reduced nicotinamide adenine dinucleotide phosphate |
| dihydropyrimidine dehydrogenase (DPD) | Sus scrofa | reduced nicotinamide adenine dinucleotide phosphateanaerobic steady state by stopped-flow; cosubstrate uracil | 0.85 | 0.0023 | 360 | pH 7.4, 20 °C | Open record: dihydropyrimidine dehydrogenase (DPD), reduced nicotinamide adenine dinucleotide phosphate |
| dihydropyrimidine dehydrogenase (DPD) | Sus scrofa | reduced nicotinamide adenine dinucleotide phosphateNADPH varied (0-1 mM); ~250 µM O2 (aerobic); 2 mM DTT | 0.043 | — | — | pH 7.4, 20 °C | Open record: dihydropyrimidine dehydrogenase (DPD), reduced nicotinamide adenine dinucleotide phosphate |
| dihydropyrimidine dehydrogenase (DPD) | Sus scrofa | reduced nicotinamide adenine dinucleotide phosphateNADPH varied (0-1 mM); ~250 µM O2 (aerobic); 2 mM DTT | 0.015 | — | — | pH 7.4, 20 °C | Open record: dihydropyrimidine dehydrogenase (DPD), reduced nicotinamide adenine dinucleotide phosphate |
| dihydropyrimidine dehydrogenase (DPD) | Sus scrofa | uracilaerobic steady state; 2 mM DTT; NADPH fixed at 200 µM | 0.73 | 0.0063 | 110 | pH 7.4, 20 °C | Open record: dihydropyrimidine dehydrogenase (DPD), uracil |
| dihydropyrimidine dehydrogenase (DPD) | Sus scrofa | uracilanaerobic steady state by stopped-flow | 0.78 | 8.7 × 10⁻⁴ | 900 | pH 7.4, 20 °C | Open record: dihydropyrimidine dehydrogenase (DPD), uracil |
| dihydropyrimidine dehydrogenase (DPD)variant C671S | Sus scrofa | 5-methyluracil | 2.4 × 10⁻⁴ | — | — | pH 7.4, 20 °C | Open record: dihydropyrimidine dehydrogenase (DPD) variant C671S, 5-methyluracil |
| dihydropyrimidine dehydrogenase (DPD)variant C671S | Sus scrofa | reduced nicotinamide adenine dinucleotide phosphate | 0.015 | 0.00931 | 2 | pH 7.4, 20 °C | Open record: dihydropyrimidine dehydrogenase (DPD) variant C671S, reduced nicotinamide adenine dinucleotide phosphate |
| dihydropyrimidine dehydrogenase (DPD)variant C671S | Sus scrofa | uracilaerobic steady state; 2 mM DTT; NADPH fixed at 200 µM | 0.03 | 0.00148 | 20 | pH 7.4, 20 °C | Open record: dihydropyrimidine dehydrogenase (DPD) variant C671S, uracil |
| dihydropyrimidine dehydrogenase (DPD)variant C671S | Sus scrofa | uracilanaerobic steady state by stopped-flow | 0.015 | 0.00548 | 3 | pH 7.4, 20 °C | Open record: dihydropyrimidine dehydrogenase (DPD) variant C671S, uracil |