Values CatLog read from this paper
Characterization of an archaeal medium-chain acyl coenzyme A synthetase from Methanosarcina acetivorans
PMID 20851904doi:10.1128/jb.00600-10Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 14 records (41 values) from this paper: medium-chain acyl-CoA synthetase (MacsMa) from Methanosarcina acetivorans.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 1 kcat/Km value marked “calc.” from kcat and Km; no printed kcat/Km was read for that record.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| medium-chain acyl-CoA synthetase (MacsMa) | Methanosarcina acetivorans | 2-methylbutyratenon-varied substrates at saturating levels | 1.58 | 8.92 | 0.18 | pH 7.5, 55 °C | Open record: medium-chain acyl-CoA synthetase (MacsMa), 2-methylbutyrate |
| medium-chain acyl-CoA synthetase (MacsMa) | Methanosarcina acetivorans | 2-methylbutyrateacyl-adenylate formation and release in the absence of CoA | 0.21 | 2.7 | 0.075 | pH 7.5, 55 °C | Open record: medium-chain acyl-CoA synthetase (MacsMa), 2-methylbutyrate |
| medium-chain acyl-CoA synthetase (MacsMa) | Methanosarcina acetivorans | 2-methylpropionate | 0.54 | 6.3 | 0.085 | pH 7.5, 55 °C | Open record: medium-chain acyl-CoA synthetase (MacsMa), 2-methylpropionate |
| medium-chain acyl-CoA synthetase (MacsMa) | Methanosarcina acetivorans | 2-methylvalerate | 0.33 | 5.3 | 0.062 | pH 7.5, 55 °C | Open record: medium-chain acyl-CoA synthetase (MacsMa), 2-methylvalerate |
| medium-chain acyl-CoA synthetase (MacsMa) | Methanosarcina acetivorans | ATPnon-varied substrates at saturating levels; Table 2 | 1.94 | 4.21 | 0.46 | pH 7.5, 55 °C | Open record: medium-chain acyl-CoA synthetase (MacsMa), ATP |
| medium-chain acyl-CoA synthetase (MacsMa) | Methanosarcina acetivorans | ATPacyl-adenylate synthesis in the absence of CoA; Table 4 | 10.67 | 6.9 | 1.54 | pH 7.5, 55 °C | Open record: medium-chain acyl-CoA synthetase (MacsMa), ATP |
| medium-chain acyl-CoA synthetase (MacsMa) | Methanosarcina acetivorans | ATPacyl-adenylate synthesis in the absence of CoA; Table 4 | 4.98 | 6.72 | 0.74 | pH 7.5, 55 °C | Open record: medium-chain acyl-CoA synthetase (MacsMa), ATP |
| medium-chain acyl-CoA synthetase (MacsMa) | Methanosarcina acetivorans | ATPacyl-adenylate synthesis in the absence of CoA; Table 4 | 1.09 | 1.66 | 0.66 | pH 7.5, 55 °C | Open record: medium-chain acyl-CoA synthetase (MacsMa), ATP |
| medium-chain acyl-CoA synthetase (MacsMa) | Methanosarcina acetivorans | ATPacyl-adenylate synthesis in the absence of CoA; Table 4 | 0.16 | 0.38 | 0.43 | pH 7.5, 55 °C | Open record: medium-chain acyl-CoA synthetase (MacsMa), ATP |
| medium-chain acyl-CoA synthetase (MacsMa) | Methanosarcina acetivorans | ATPacyl-adenylate synthesis in the absence of CoA; Table 4 | 0.39 | 3.51 | 0.11 | pH 7.5, 55 °C | Open record: medium-chain acyl-CoA synthetase (MacsMa), ATP |
| medium-chain acyl-CoA synthetase (MacsMa) | Methanosarcina acetivorans | butyrate | 2.01 | 27.9 | 0.073 | pH 7.5, 55 °C | Open record: medium-chain acyl-CoA synthetase (MacsMa), butyrate |
| medium-chain acyl-CoA synthetase (MacsMa) | Methanosarcina acetivorans | coenzyme A | 2.15 | 4.09 | 0.53 | pH 7.5, 55 °C | Open record: medium-chain acyl-CoA synthetase (MacsMa), coenzyme A |
| medium-chain acyl-CoA synthetase (MacsMa) | Methanosarcina acetivorans | propionate | 4.78 | 29.1 | 0.164 | pH 7.5, 55 °C | Open record: medium-chain acyl-CoA synthetase (MacsMa), propionate |
| medium-chain acyl-CoA synthetase (MacsMa)variant K256L | Methanosarcina acetivorans | propionate | 0.22 | 101.1 | 0.0022 calc. | pH 7.5, 55 °C | Open record: medium-chain acyl-CoA synthetase (MacsMa) variant K256L, propionate |