CatLogenzyme kinetics catalog

Values CatLog read from this paper

Essential residues for the enzyme activity of ATP-dependent MurE ligase from Mycobacterium tuberculosis

PMID 21153518doi:10.1007/s13238-010-0132-9Open in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 20 records (27 values) from this paper: MurE ligase (UDP-MurNAc-tripeptide ligase) from Mycobacterium tuberculosis.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 7 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.

EC 6.3.2.13

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)Conditions
MurE ligase (UDP-MurNAc-tripeptide ligase)Mycobacterium tuberculosisATP1.30.01776 calc.pH 8.5, 37 °COpen
MurE ligase (UDP-MurNAc-tripeptide ligase)Mycobacterium tuberculosismeso-2,6-diaminopimelic acid—0.078—pH 8.5, 37 °COpen
MurE ligase (UDP-MurNAc-tripeptide ligase)Mycobacterium tuberculosisUDP-N-acetylmuramoyl-L-alanyl-D-glutamate—0.024—pH 8.5, 37 °COpen
MurE ligase (UDP-MurNAc-tripeptide ligase)variant D392AMycobacterium tuberculosisATP0.10.11.0 calc.pH 8.5, 37 °COpen
MurE ligase (UDP-MurNAc-tripeptide ligase)variant D392AMycobacterium tuberculosismeso-2,6-diaminopimelic acid—0.113—pH 8.5, 37 °COpen
MurE ligase (UDP-MurNAc-tripeptide ligase)variant D392AMycobacterium tuberculosisUDP-N-acetylmuramoyl-L-alanyl-D-glutamate—0.016—pH 8.5, 37 °COpen
MurE ligase (UDP-MurNAc-tripeptide ligase)variant E220AMycobacterium tuberculosisATP0.570.1234.6 calc.pH 8.5, 37 °COpen
MurE ligase (UDP-MurNAc-tripeptide ligase)variant E220AMycobacterium tuberculosismeso-2,6-diaminopimelic acid—0.052—pH 8.5, 37 °COpen
MurE ligase (UDP-MurNAc-tripeptide ligase)variant E220AMycobacterium tuberculosisUDP-N-acetylmuramoyl-L-alanyl-D-glutamate—0.148—pH 8.5, 37 °COpen
MurE ligase (UDP-MurNAc-tripeptide ligase)variant K157AMycobacterium tuberculosisATP0.0830.00810 calc.pH 8.5, 37 °COpen
MurE ligase (UDP-MurNAc-tripeptide ligase)variant K157AMycobacterium tuberculosismeso-2,6-diaminopimelic acid—0.012—pH 8.5, 37 °COpen
MurE ligase (UDP-MurNAc-tripeptide ligase)variant N449DMycobacterium tuberculosisATP0.050.060.83 calc.pH 8.5, 37 °COpen
MurE ligase (UDP-MurNAc-tripeptide ligase)variant N449DMycobacterium tuberculosismeso-2,6-diaminopimelic acid—0.324—pH 8.5, 37 °COpen
MurE ligase (UDP-MurNAc-tripeptide ligase)variant N449DMycobacterium tuberculosisUDP-N-acetylmuramoyl-L-alanyl-D-glutamate—0.007—pH 8.5, 37 °COpen
MurE ligase (UDP-MurNAc-tripeptide ligase)variant R451AMycobacterium tuberculosisATP0.0670.0154.4 calc.pH 8.5, 37 °COpen
MurE ligase (UDP-MurNAc-tripeptide ligase)variant R451AMycobacterium tuberculosismeso-2,6-diaminopimelic acid—0.473—pH 8.5, 37 °COpen
MurE ligase (UDP-MurNAc-tripeptide ligase)variant R451AMycobacterium tuberculosisUDP-N-acetylmuramoyl-L-alanyl-D-glutamate—0.005—pH 8.5, 37 °COpen
MurE ligase (UDP-MurNAc-tripeptide ligase)variant ΔN24 (MurEtr)Mycobacterium tuberculosisATP1.120.02153 calc.pH 8.5, 37 °COpen
MurE ligase (UDP-MurNAc-tripeptide ligase)variant ΔN24 (MurEtr)Mycobacterium tuberculosismeso-2,6-diaminopimelic acid—0.065—pH 8.5, 37 °COpen
MurE ligase (UDP-MurNAc-tripeptide ligase)variant ΔN24 (MurEtr)Mycobacterium tuberculosisUDP-N-acetylmuramoyl-L-alanyl-D-glutamate—0.019—pH 8.5, 37 °COpen