CatLogenzyme kinetics catalog

Values CatLog read from this paper

Identification of macrodomain proteins as novel O-acetyl-ADP-ribose deacetylases

PMID 21257746doi:10.1074/jbc.m110.206771Open in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 25 records (39 values) from this paper: MacroD1, SAV0325 (sirtuin-linked MacroD-like protein), YmdB and 1 more from Homo sapiens, Staphylococcus aureus, Escherichia coli.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 4 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.

EC 3.1.1.106

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)Conditions
MacroD1Homo sapiensO-acetyl-ADP-ribose50-200 mM Tris-HCl or 50 mM phosphate buffer; pH 7.30.20.3750.533pH 7.3, 23 °COpen
MacroD1Homo sapiensO-acetyl-ADP-ribose50-200 mM Tris-HCl or 50 mM phosphate buffer; pH 7.30.198——pH 7.3, 23 °COpen
MacroD1Homo sapiensO-acetyl-ADP-ribose50 mM Tris-HCl pH 7.3; 0.5 µM MacroD1; no ADPr (control)0.2020.37580.538 calc.pH 7.3, 23 °COpen
MacroD1Homo sapiensO-acetyl-ADP-ribose50 mM Tris-HCl pH 7.3; 0.5 µM MacroD1; apparent Km0.2040.59090.345 calc.pH 7.3, 23 °COpen
MacroD1Homo sapiensO-acetyl-ADP-ribose50 mM Tris-HCl pH 7.3; 0.5 µM MacroD1; apparent Km0.2010.98660.204 calc.pH 7.3, 23 °COpen
MacroD1Homo sapiensO-acetyl-ADP-ribose50 mM Tris-HCl pH 7.3; 0.5 µM MacroD1; apparent Km0.1961.3500.145 calc.pH 7.3, 23 °COpen
MacroD1variant C199AHomo sapiensO-acetyl-ADP-ribose0.179——pH 7.3, 23 °COpen
MacroD1variant D160AHomo sapiensO-acetyl-ADP-ribose0.114——pH 7.3, 23 °COpen
MacroD1variant D167AHomo sapiensO-acetyl-ADP-ribose0.136——pH 7.3, 23 °COpen
MacroD1variant D184AHomo sapiensO-acetyl-ADP-ribose0.078——pH 7.3, 23 °COpen
MacroD1variant H188AHomo sapiensO-acetyl-ADP-ribose0.114——pH 7.3, 23 °COpen
MacroD1variant N171AHomo sapiensO-acetyl-ADP-ribose0.082——pH 7.3, 23 °COpen
MacroD1variant N174AHomo sapiensO-acetyl-ADP-ribose0.113——pH 7.3, 23 °COpen
MacroD1variant N174A/D184AHomo sapiensO-acetyl-ADP-ribose0.014——pH 7.3, 23 °COpen
MacroD1variant S176AHomo sapiensO-acetyl-ADP-ribose0.195——pH 7.3, 23 °COpen
MacroD1variant S268AHomo sapiensO-acetyl-ADP-ribose0.153——pH 7.3, 23 °COpen
MacroD1variant T226AHomo sapiensO-acetyl-ADP-ribose0.204——pH 7.3, 23 °COpen
MacroD1variant ΔN58aaHomo sapiensO-acetyl-ADP-ribose0.210.2730.77pH 7.3, 23 °COpen
MacroD2Homo sapiensO-acetyl-ADP-ribose0.120.1071.1pH 7.3, 23 °COpen
SAV0325 (sirtuin-linked MacroD-like protein)Staphylococcus aureusO-acetyl-ADP-riboseTable 13.6721.84pH 7.3, 23 °COpen
SAV0325 (sirtuin-linked MacroD-like protein)Staphylococcus aureusO-acetyl-ADP-riboseTable 22.77——pH 7.3, 23 °COpen
SAV0325 (sirtuin-linked MacroD-like protein)variant D122AStaphylococcus aureusO-acetyl-ADP-ribose0.679——pH 7.3, 23 °COpen
SAV0325 (sirtuin-linked MacroD-like protein)variant G214EStaphylococcus aureusO-acetyl-ADP-ribose0.007——pH 7.3, 23 °COpen
YmdBEscherichia coliO-acetyl-ADP-ribose0.480.271.78pH 7.3, 23 °COpen
YmdBvariant G124EEscherichia coliO-acetyl-ADP-ribose0.005——pH 7.3, 23 °COpen