Values CatLog read from this paper
Probing the molecular basis of substrate specificity, stereospecificity, and catalysis in the class II pyruvate aldolase, BphI
PMID 21425833doi:10.1021/bi101947gOpen in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 27 records (63 values) from this paper: BphI from Burkholderia xenovorans.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| BphI | Burkholderia xenovorans | (4S)-4-hydroxy-2-oxopentanoate | 4.07 | 0.089 | 44.7 | pH 8, 25 °C | Open record: BphI, (4S)-4-hydroxy-2-oxopentanoate |
| BphI | Burkholderia xenovorans | hexanal | — | — | 1 × 10⁻⁵ | pH 8, 25 °C | Open record: BphI, hexanal |
| BphI | Burkholderia xenovorans | pyruvate | 1.2 | 13 | 0.0915 | pH 8, 25 °C | Open record: BphI, pyruvate |
| BphIvariant H20A | Burkholderia xenovorans | (4S)-4-hydroxy-2-oxopentanoate | 0.099 | 0.222 | 0.45 | pH 8, 25 °C | Open record: BphI variant H20A, (4S)-4-hydroxy-2-oxopentanoate |
| BphIvariant H20S | Burkholderia xenovorans | (4S)-4-hydroxy-2-oxopentanoate | 0.089 | 0.228 | 0.34 | pH 8, 25 °C | Open record: BphI variant H20S, (4S)-4-hydroxy-2-oxopentanoate |
| BphIvariant L87A | Burkholderia xenovorans | acetaldehyde | — | — | 4.2 × 10⁻⁴ | pH 8, 25 °C | Open record: BphI variant L87A, acetaldehyde |
| BphIvariant L87A | Burkholderia xenovorans | butanal | 0.26 | 323 | 7.9 × 10⁻⁴ | pH 8, 25 °C | Open record: BphI variant L87A, butanal |
| BphIvariant L87A | Burkholderia xenovorans | hexanal | — | — | 1 × 10⁻⁵ | pH 8, 25 °C | Open record: BphI variant L87A, hexanal |
| BphIvariant L87A | Burkholderia xenovorans | pentanal | — | — | 3.3 × 10⁻⁴ | pH 8, 25 °C | Open record: BphI variant L87A, pentanal |
| BphIvariant L87A | Burkholderia xenovorans | propanal | 0.29 | 16.8 | 0.0175 | pH 8, 25 °C | Open record: BphI variant L87A, propanal |
| BphIvariant L87A | Burkholderia xenovorans | pyruvate | 0.32 | 20.2 | 0.0159 | pH 8, 25 °C | Open record: BphI variant L87A, pyruvate |
| BphIvariant L87A/L89A | Burkholderia xenovorans | acetaldehyde | — | — | 4.9 × 10⁻⁴ | pH 8, 25 °C | Open record: BphI variant L87A/L89A, acetaldehyde |
| BphIvariant L87A/L89A | Burkholderia xenovorans | butanal | 0.3 | 289 | 0.00104 | pH 8, 25 °C | Open record: BphI variant L87A/L89A, butanal |
| BphIvariant L87A/L89A | Burkholderia xenovorans | hexanal | — | — | 1 × 10⁻⁴ | pH 8, 25 °C | Open record: BphI variant L87A/L89A, hexanal |
| BphIvariant L87A/L89A | Burkholderia xenovorans | pentanal | 0.13 | 33.3 | 0.0038 | pH 8, 25 °C | Open record: BphI variant L87A/L89A, pentanal |
| BphIvariant L87A/L89A | Burkholderia xenovorans | propanal | — | — | 0.00673 | pH 8, 25 °C | Open record: BphI variant L87A/L89A, propanal |
| BphIvariant L89A | Burkholderia xenovorans | acetaldehyde | 0.94 | 80.5 | 0.0117 | pH 8, 25 °C | Open record: BphI variant L89A, acetaldehyde |
| BphIvariant L89A | Burkholderia xenovorans | butanal | 0.64 | 49.2 | 0.0129 | pH 8, 25 °C | Open record: BphI variant L89A, butanal |
| BphIvariant L89A | Burkholderia xenovorans | hexanal | — | — | 1.3 × 10⁻⁴ | pH 8, 25 °C | Open record: BphI variant L89A, hexanal |
| BphIvariant L89A | Burkholderia xenovorans | pentanal | 0.58 | 24.8 | 0.0233 | pH 8, 25 °C | Open record: BphI variant L89A, pentanal |
| BphIvariant L89A | Burkholderia xenovorans | propanal | 0.63 | 40.2 | 0.0156 | pH 8, 25 °C | Open record: BphI variant L89A, propanal |
| BphIvariant L89A | Burkholderia xenovorans | pyruvate | 0.64 | 11 | 0.0587 | pH 8, 25 °C | Open record: BphI variant L89A, pyruvate |
| BphIvariant R16K | Burkholderia xenovorans | (4S)-4-hydroxy-2-oxopentanoate | — | — | 0.012 | pH 8, 25 °C | Open record: BphI variant R16K, (4S)-4-hydroxy-2-oxopentanoate |
| BphIvariant Y290F | Burkholderia xenovorans | (4R)-4-hydroxy-2-oxopentanoate | 0.131 | 0.013 | 10.4 | pH 8, 25 °C | Open record: BphI variant Y290F, (4R)-4-hydroxy-2-oxopentanoate |
| BphIvariant Y290F | Burkholderia xenovorans | (4S)-4-hydroxy-2-oxopentanoate | 0.132 | 0.013 | 12.6 | pH 8, 25 °C | Open record: BphI variant Y290F, (4S)-4-hydroxy-2-oxopentanoate |
| BphIvariant Y290S | Burkholderia xenovorans | (4R)-4-hydroxy-2-oxopentanoate | 0.17 | 0.012 | 14.1 | pH 8, 25 °C | Open record: BphI variant Y290S, (4R)-4-hydroxy-2-oxopentanoate |
| BphIvariant Y290S | Burkholderia xenovorans | (4S)-4-hydroxy-2-oxopentanoate | 0.171 | 0.013 | 13.4 | pH 8, 25 °C | Open record: BphI variant Y290S, (4S)-4-hydroxy-2-oxopentanoate |