EC 4.1.3.43: 4-hydroxy-2-oxohexanoate aldolase
LyasesOpen in Browse
CatLog read 41 records for EC 4.1.3.43 from 3 papers, for 2 organisms. Names in these papers: BphI, aldolase TTHB246, aldolase BphI.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units.
CatLog also holds 14 records for EC 4.1.3.43 that are not yet re-read: from its earlier version, built from the enzyme databases and partly checked against the papers. All records for EC 4.1.3.43 in Browse.
Papers
| Paper | Organisms | Records |
|---|---|---|
| Rational design of stereoselectivity in the class II pyruvate aldolase BphI | Burkholderia xenovorans | 24 |
| Protein-protein interactions and substrate channeling in orthologous and chimeric aldolase-dehydrogenase complexes | Burkholderia xenovorans, Thermus thermophilus | 16 |
| Probing the molecular basis of substrate specificity, stereospecificity, and catalysis in the class II pyruvate aldolase, BphI | Burkholderia xenovorans | 1 |
Records
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Paper | Open record |
|---|---|---|---|---|---|---|---|---|
| aldolase BphI | Burkholderia xenovorans | 4-hydroxy-2-oxohexanoateBphI-TTHB247 complex; in complex with TTHB247 | 2.1 | 0.199 | 11 | pH 8, 25 °C | PMID 22316175 | Open record: aldolase BphI, 4-hydroxy-2-oxohexanoate |
| aldolase BphI | Burkholderia xenovorans | 4-hydroxy-2-oxohexanoateBphI-BphJ complex; in complex with BphJ | — | — | 33 | pH 8, 25 °C | PMID 22316175 | Open record: aldolase BphI, 4-hydroxy-2-oxohexanoate |
| aldolase BphI | Burkholderia xenovorans | 4-hydroxy-2-oxopentanoate | 4.1 | — | 46 | pH 8, 25 °C | PMID 22316175 | Open record: aldolase BphI, 4-hydroxy-2-oxopentanoate |
| aldolase TTHB246 | Thermus thermophilus | 4-hydroxy-2-oxohexanoateTTHB246 alone (uncomplexed) | 1.1 | 0.21 | 5.2 | pH 8, 25 °C | PMID 22316175 | Open record: aldolase TTHB246, 4-hydroxy-2-oxohexanoate |
| aldolase TTHB246 | Thermus thermophilus | 4-hydroxy-2-oxohexanoateTTHB246-TTHB247 complex; in complex with TTHB247 | 0.5 | 0.037 | 13 | pH 8, 25 °C | PMID 22316175 | Open record: aldolase TTHB246, 4-hydroxy-2-oxohexanoate |
| aldolase TTHB246 | Thermus thermophilus | 4-hydroxy-2-oxohexanoateTTHB246-BphJ complex; in complex with BphJ | 2.2 | 0.218 | 10 | pH 8, 25 °C | PMID 22316175 | Open record: aldolase TTHB246, 4-hydroxy-2-oxohexanoate |
| aldolase TTHB246 | Thermus thermophilus | 4-hydroxy-2-oxopentanoate1 mM CoCl2; TTHB246 alone (uncomplexed); Table 4 | 1 | 0.206 | 5 | pH 8, 25 °C | PMID 22316175 | Open record: aldolase TTHB246, 4-hydroxy-2-oxopentanoate |
| aldolase TTHB246 | Thermus thermophilus | 4-hydroxy-2-oxopentanoate1 mM CoCl2; TTHB246-TTHB247 complex; in complex with TTHB247 | 0.6 | 0.041 | 14 | pH 8, 25 °C | PMID 22316175 | Open record: aldolase TTHB246, 4-hydroxy-2-oxopentanoate |
| aldolase TTHB246 | Thermus thermophilus | 4-hydroxy-2-oxopentanoate1 mM CoCl2; TTHB246-BphJ complex; in complex with BphJ | 2.4 | 0.257 | 9.4 | pH 8, 25 °C | PMID 22316175 | Open record: aldolase TTHB246, 4-hydroxy-2-oxopentanoate |
| aldolase TTHB246 | Thermus thermophilus | 4-hydroxy-2-oxopentanoateTTHB246-TTHB247 complex; in complex with TTHB247 | 0.3 | 0.123 | 2.9 | pH 8 | PMID 22316175 | Open record: aldolase TTHB246, 4-hydroxy-2-oxopentanoate |
| aldolase TTHB246 | Thermus thermophilus | 4-hydroxy-2-oxopentanoateTTHB246-BphJ complex; in complex with BphJ | 2.4 | 0.253 | 9.3 | pH 8 | PMID 22316175 | Open record: aldolase TTHB246, 4-hydroxy-2-oxopentanoate |
| aldolase TTHB246 | Thermus thermophilus | 4-hydroxy-2-oxopentanoate2 mM HOPA fixed; 30 μg enzyme; TTHB246 alone (uncomplexed) | 1 | — | — | pH 8, 25 °C | PMID 22316175 | Open record: aldolase TTHB246, 4-hydroxy-2-oxopentanoate |
| aldolase TTHB246 | Thermus thermophilus | 4-hydroxy-2-oxopentanoate2 mM HOPA fixed; 30 μg enzyme; TTHB246 alone (uncomplexed) | 0.79 | — | — | pH 8, 25 °C | PMID 22316175 | Open record: aldolase TTHB246, 4-hydroxy-2-oxopentanoate |
| aldolase TTHB246 | Thermus thermophilus | 4-hydroxy-2-oxopentanoate2 mM HOPA fixed; 30 μg enzyme; TTHB246 alone (uncomplexed) | 0.57 | — | — | pH 8, 25 °C | PMID 22316175 | Open record: aldolase TTHB246, 4-hydroxy-2-oxopentanoate |
| aldolase TTHB246 | Thermus thermophilus | 4-hydroxy-2-oxopentanoate2 mM HOPA fixed; 30 μg enzyme; TTHB246 alone (uncomplexed) | 0.13 | — | — | pH 8, 25 °C | PMID 22316175 | Open record: aldolase TTHB246, 4-hydroxy-2-oxopentanoate |
| aldolase TTHB246 | Thermus thermophilus | 4-hydroxy-2-oxopentanoate2 mM HOPA fixed; 30 μg enzyme; TTHB246 alone (uncomplexed) | 0.08 | — | — | pH 8, 25 °C | PMID 22316175 | Open record: aldolase TTHB246, 4-hydroxy-2-oxopentanoate |
| BphI | Burkholderia xenovorans | 4(S)-hydroxy-2-oxopentanoate | 4 | — | — | pH 8, 25 °C | PMID 22081904 | Open record: BphI, 4(S)-hydroxy-2-oxopentanoate |
| BphI | Burkholderia xenovorans | oxaloacetate | 16.3 | 7.4 | 2.2 | pH 8, 25 °C | PMID 22081904 | Open record: BphI, oxaloacetate |
| BphIvariant L87A/L89A | Burkholderia xenovorans | hexanal | — | — | 1 × 10⁻⁴ | pH 8, 25 °C | PMID 21425833 | Open record: BphI variant L87A/L89A, hexanal |
| BphIvariant L87N | Burkholderia xenovorans | oxaloacetate | 0.05 | 0.21 | 0.254 | pH 8, 25 °C | PMID 22081904 | Open record: BphI variant L87N, oxaloacetate |
| BphIvariant L87N/Y290F | Burkholderia xenovorans | 4(R)-hydroxy-2-oxopentanoate | 0.098 | 0.757 | 0.13 | pH 8, 25 °C | PMID 22081904 | Open record: BphI variant L87N/Y290F, 4(R)-hydroxy-2-oxopentanoate |
| BphIvariant L87N/Y290F | Burkholderia xenovorans | acetaldehyde | 0.3 | 70.7 | 0.0043 | pH 8, 25 °C | PMID 22081904 | Open record: BphI variant L87N/Y290F, acetaldehyde |
| BphIvariant L87N/Y290F | Burkholderia xenovorans | butyraldehyde | — | — | 1.6 × 10⁻⁴ | pH 8, 25 °C | PMID 22081904 | Open record: BphI variant L87N/Y290F, butyraldehyde |
| BphIvariant L87N/Y290F | Burkholderia xenovorans | oxaloacetate | 0.08 | 0.84 | 0.098 | pH 8, 25 °C | PMID 22081904 | Open record: BphI variant L87N/Y290F, oxaloacetate |
| BphIvariant L87N/Y290F | Burkholderia xenovorans | pentaldehyde | — | — | 4 × 10⁻⁵ | pH 8, 25 °C | PMID 22081904 | Open record: BphI variant L87N/Y290F, pentaldehyde |
| BphIvariant L87N/Y290F | Burkholderia xenovorans | propionaldehyde | 0.1 | 54.9 | 0.0018 | pH 8, 25 °C | PMID 22081904 | Open record: BphI variant L87N/Y290F, propionaldehyde |
| BphIvariant L87N/Y290F | Burkholderia xenovorans | pyruvate | 0.11 | 30.2 | 0.0036 | pH 8, 25 °C | PMID 22081904 | Open record: BphI variant L87N/Y290F, pyruvate |
| BphIvariant L87W | Burkholderia xenovorans | oxaloacetate | — | — | 0.0058 | pH 8, 25 °C | PMID 22081904 | Open record: BphI variant L87W, oxaloacetate |
| BphIvariant L87W/Y290F | Burkholderia xenovorans | 4(R)-hydroxy-2-oxopentanoate | 0.043 | 0.435 | 0.1 | pH 8, 25 °C | PMID 22081904 | Open record: BphI variant L87W/Y290F, 4(R)-hydroxy-2-oxopentanoate |
| BphIvariant L87W/Y290F | Burkholderia xenovorans | acetaldehyde | 0.59 | 86.3 | 0.0069 | pH 8, 25 °C | PMID 22081904 | Open record: BphI variant L87W/Y290F, acetaldehyde |
| BphIvariant L87W/Y290F | Burkholderia xenovorans | butyraldehyde | — | — | 3.1 × 10⁻⁴ | pH 8, 25 °C | PMID 22081904 | Open record: BphI variant L87W/Y290F, butyraldehyde |
| BphIvariant L87W/Y290F | Burkholderia xenovorans | oxaloacetate | 0.4 | 6.2 | 0.064 | pH 8, 25 °C | PMID 22081904 | Open record: BphI variant L87W/Y290F, oxaloacetate |
| BphIvariant L87W/Y290F | Burkholderia xenovorans | pentaldehyde | — | — | 5.1 × 10⁻⁵ | pH 8, 25 °C | PMID 22081904 | Open record: BphI variant L87W/Y290F, pentaldehyde |
| BphIvariant L87W/Y290F | Burkholderia xenovorans | propionaldehyde | 0.14 | 24.4 | 0.0057 | pH 8, 25 °C | PMID 22081904 | Open record: BphI variant L87W/Y290F, propionaldehyde |
| BphIvariant L87W/Y290F | Burkholderia xenovorans | pyruvate | 0.16 | 38.2 | 0.0043 | pH 8, 25 °C | PMID 22081904 | Open record: BphI variant L87W/Y290F, pyruvate |
| BphIvariant Y290F | Burkholderia xenovorans | acetaldehyde | 0.2 | 81 | 0.0025 | pH 8, 25 °C | PMID 22081904 | Open record: BphI variant Y290F, acetaldehyde |
| BphIvariant Y290F | Burkholderia xenovorans | butyraldehyde | 0.16 | 104 | 0.0015 | pH 8, 25 °C | PMID 22081904 | Open record: BphI variant Y290F, butyraldehyde |
| BphIvariant Y290F | Burkholderia xenovorans | oxaloacetate | 0.27 | 0.92 | 0.53 | pH 8, 25 °C | PMID 22081904 | Open record: BphI variant Y290F, oxaloacetate |
| BphIvariant Y290F | Burkholderia xenovorans | pentaldehyde | 0.14 | 80.5 | 0.0017 | pH 8, 25 °C | PMID 22081904 | Open record: BphI variant Y290F, pentaldehyde |
| BphIvariant Y290F | Burkholderia xenovorans | propionaldehyde | 0.16 | 81.2 | 0.002 | pH 8, 25 °C | PMID 22081904 | Open record: BphI variant Y290F, propionaldehyde |
| BphIvariant Y290F | Burkholderia xenovorans | pyruvate | 0.19 | 20 | 0.0084 | pH 8, 25 °C | PMID 22081904 | Open record: BphI variant Y290F, pyruvate |