Values CatLog read from this paper

Rational design of stereoselectivity in the class II pyruvate aldolase BphI

PMID 22081904doi:10.1021/ja208754rOpen in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 24 records (60 values) from this paper: BphI from Burkholderia xenovorans.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.

EC 4.1.3.43

Records read from this paper
Enzyme	Organism	Substrate	k_cat (s⁻¹)	K_m (mM)	k_cat/K_m (mM⁻¹ s⁻¹)	Conditions	Open record
BphI	Burkholderia xenovorans	4(S)-hydroxy-2-oxopentanoate	4	—	—	pH 8, 25 °C	Open record: BphI, 4(S)-hydroxy-2-oxopentanoate
BphI	Burkholderia xenovorans	oxaloacetate	16.3	7.4	2.2	pH 8, 25 °C	Open record: BphI, oxaloacetate
BphIvariant L87N	Burkholderia xenovorans	oxaloacetate	0.05	0.21	0.254	pH 8, 25 °C	Open record: BphI variant L87N, oxaloacetate
BphIvariant L87N/Y290F	Burkholderia xenovorans	4(R)-hydroxy-2-oxopentanoate	0.098	0.757	0.13	pH 8, 25 °C	Open record: BphI variant L87N/Y290F, 4(R)-hydroxy-2-oxopentanoate
BphIvariant L87N/Y290F	Burkholderia xenovorans	acetaldehyde	0.3	70.7	0.0043	pH 8, 25 °C	Open record: BphI variant L87N/Y290F, acetaldehyde
BphIvariant L87N/Y290F	Burkholderia xenovorans	butyraldehyde	—	—	1.6 × 10⁻⁴	pH 8, 25 °C	Open record: BphI variant L87N/Y290F, butyraldehyde
BphIvariant L87N/Y290F	Burkholderia xenovorans	oxaloacetate	0.08	0.84	0.098	pH 8, 25 °C	Open record: BphI variant L87N/Y290F, oxaloacetate
BphIvariant L87N/Y290F	Burkholderia xenovorans	pentaldehyde	—	—	4 × 10⁻⁵	pH 8, 25 °C	Open record: BphI variant L87N/Y290F, pentaldehyde
BphIvariant L87N/Y290F	Burkholderia xenovorans	propionaldehyde	0.1	54.9	0.0018	pH 8, 25 °C	Open record: BphI variant L87N/Y290F, propionaldehyde
BphIvariant L87N/Y290F	Burkholderia xenovorans	pyruvate	0.11	30.2	0.0036	pH 8, 25 °C	Open record: BphI variant L87N/Y290F, pyruvate
BphIvariant L87W	Burkholderia xenovorans	oxaloacetate	—	—	0.0058	pH 8, 25 °C	Open record: BphI variant L87W, oxaloacetate
BphIvariant L87W/Y290F	Burkholderia xenovorans	4(R)-hydroxy-2-oxopentanoate	0.043	0.435	0.1	pH 8, 25 °C	Open record: BphI variant L87W/Y290F, 4(R)-hydroxy-2-oxopentanoate
BphIvariant L87W/Y290F	Burkholderia xenovorans	acetaldehyde	0.59	86.3	0.0069	pH 8, 25 °C	Open record: BphI variant L87W/Y290F, acetaldehyde
BphIvariant L87W/Y290F	Burkholderia xenovorans	butyraldehyde	—	—	3.1 × 10⁻⁴	pH 8, 25 °C	Open record: BphI variant L87W/Y290F, butyraldehyde
BphIvariant L87W/Y290F	Burkholderia xenovorans	oxaloacetate	0.4	6.2	0.064	pH 8, 25 °C	Open record: BphI variant L87W/Y290F, oxaloacetate
BphIvariant L87W/Y290F	Burkholderia xenovorans	pentaldehyde	—	—	5.1 × 10⁻⁵	pH 8, 25 °C	Open record: BphI variant L87W/Y290F, pentaldehyde
BphIvariant L87W/Y290F	Burkholderia xenovorans	propionaldehyde	0.14	24.4	0.0057	pH 8, 25 °C	Open record: BphI variant L87W/Y290F, propionaldehyde
BphIvariant L87W/Y290F	Burkholderia xenovorans	pyruvate	0.16	38.2	0.0043	pH 8, 25 °C	Open record: BphI variant L87W/Y290F, pyruvate
BphIvariant Y290F	Burkholderia xenovorans	acetaldehyde	0.2	81	0.0025	pH 8, 25 °C	Open record: BphI variant Y290F, acetaldehyde
BphIvariant Y290F	Burkholderia xenovorans	butyraldehyde	0.16	104	0.0015	pH 8, 25 °C	Open record: BphI variant Y290F, butyraldehyde
BphIvariant Y290F	Burkholderia xenovorans	oxaloacetate	0.27	0.92	0.53	pH 8, 25 °C	Open record: BphI variant Y290F, oxaloacetate
BphIvariant Y290F	Burkholderia xenovorans	pentaldehyde	0.14	80.5	0.0017	pH 8, 25 °C	Open record: BphI variant Y290F, pentaldehyde
BphIvariant Y290F	Burkholderia xenovorans	propionaldehyde	0.16	81.2	0.002	pH 8, 25 °C	Open record: BphI variant Y290F, propionaldehyde
BphIvariant Y290F	Burkholderia xenovorans	pyruvate	0.19	20	0.0084	pH 8, 25 °C	Open record: BphI variant Y290F, pyruvate