Values CatLog read from this paper
A tale of two isomerases: compact versus extended active sites in ketosteroid isomerase and phosphoglucose isomerase
PMID 21970785doi:10.1021/bi201089vOpen in Browse
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CatLog read 16 records (48 values) from this paper: phosphoglucose isomerase (hPGI), ketosteroid isomerase (ppKSI, Δ5-3-ketosteroid isomerase) from Homo sapiens, Pseudomonas putida.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| ketosteroid isomerase (ppKSI, Δ5-3-ketosteroid isomerase) | Pseudomonas putida | 5-androstene-3,17-dione | 3.6 × 10⁴ | 0.089 | 4.1 × 10⁵ | pH 7, 21 °C | Open record: ketosteroid isomerase (ppKSI, Δ5-3-ketosteroid isomerase), 5-androstene-3,17-dione |
| ketosteroid isomerase (ppKSI, Δ5-3-ketosteroid isomerase)variant E39Q | Pseudomonas putida | 5-androstene-3,17-dione | 1.9 × 10⁴ | 0.11 | 1.7 × 10⁵ | pH 7, 21 °C | Open record: ketosteroid isomerase (ppKSI, Δ5-3-ketosteroid isomerase) variant E39Q, 5-androstene-3,17-dione |
| ketosteroid isomerase (ppKSI, Δ5-3-ketosteroid isomerase)variant M105A | Pseudomonas putida | 5-androstene-3,17-dione | 2.4 × 10³ | 0.044 | 5.4 × 10⁴ | pH 7, 21 °C | Open record: ketosteroid isomerase (ppKSI, Δ5-3-ketosteroid isomerase) variant M105A, 5-androstene-3,17-dione |
| ketosteroid isomerase (ppKSI, Δ5-3-ketosteroid isomerase)variant M31A | Pseudomonas putida | 5-androstene-3,17-dione | 1.8 × 10⁴ | 0.089 | 2 × 10⁵ | pH 7, 21 °C | Open record: ketosteroid isomerase (ppKSI, Δ5-3-ketosteroid isomerase) variant M31A, 5-androstene-3,17-dione |
| ketosteroid isomerase (ppKSI, Δ5-3-ketosteroid isomerase)variant M84A | Pseudomonas putida | 5-androstene-3,17-dione | 3.4 × 10⁴ | 0.081 | 4.2 × 10⁵ | pH 7, 21 °C | Open record: ketosteroid isomerase (ppKSI, Δ5-3-ketosteroid isomerase) variant M84A, 5-androstene-3,17-dione |
| phosphoglucose isomerase (hPGI) | Homo sapiens | fructose 6-phosphate | 500 | 0.29 | 1.7 × 10³ | pH 7.5, 21 °C | Open record: phosphoglucose isomerase (hPGI), fructose 6-phosphate |
| phosphoglucose isomerase (hPGI)variant D511N | Homo sapiens | fructose 6-phosphate | 1.3 | 0.18 | 7.4 | pH 7.5, 21 °C | Open record: phosphoglucose isomerase (hPGI) variant D511N, fructose 6-phosphate |
| phosphoglucose isomerase (hPGI)variant E495Q | Homo sapiens | fructose 6-phosphate | 1.6 | 0.23 | 6.7 | pH 7.5, 21 °C | Open record: phosphoglucose isomerase (hPGI) variant E495Q, fructose 6-phosphate |
| phosphoglucose isomerase (hPGI)variant H100L | Homo sapiens | fructose 6-phosphate | 0.6 | 0.21 | 2.7 | pH 7.5, 21 °C | Open record: phosphoglucose isomerase (hPGI) variant H100L, fructose 6-phosphate |
| phosphoglucose isomerase (hPGI)variant H396L | Homo sapiens | fructose 6-phosphate | 21 | 0.29 | 72 | pH 7.5, 21 °C | Open record: phosphoglucose isomerase (hPGI) variant H396L, fructose 6-phosphate |
| phosphoglucose isomerase (hPGI)variant N154Q | Homo sapiens | fructose 6-phosphate | 470 | 1.04 | 450 | pH 7.5, 21 °C | Open record: phosphoglucose isomerase (hPGI) variant N154Q, fructose 6-phosphate |
| phosphoglucose isomerase (hPGI)variant N386A | Homo sapiens | fructose 6-phosphate | 470 | 0.78 | 610 | pH 7.5, 21 °C | Open record: phosphoglucose isomerase (hPGI) variant N386A, fructose 6-phosphate |
| phosphoglucose isomerase (hPGI)variant Q388A | Homo sapiens | fructose 6-phosphate | 45 | 0.5 | 90 | pH 7.5, 21 °C | Open record: phosphoglucose isomerase (hPGI) variant Q388A, fructose 6-phosphate |
| phosphoglucose isomerase (hPGI)variant S185A | Homo sapiens | fructose 6-phosphate | 360 | 0.73 | 490 | pH 7.5, 21 °C | Open record: phosphoglucose isomerase (hPGI) variant S185A, fructose 6-phosphate |
| phosphoglucose isomerase (hPGI)variant Y274F | Homo sapiens | fructose 6-phosphate | 240 | 0.3 | 790 | pH 7.5, 21 °C | Open record: phosphoglucose isomerase (hPGI) variant Y274F, fructose 6-phosphate |
| phosphoglucose isomerase (hPGI)variant Y341F | Homo sapiens | fructose 6-phosphate | 340 | 0.3 | 1.1 × 10³ | pH 7.5, 21 °C | Open record: phosphoglucose isomerase (hPGI) variant Y341F, fructose 6-phosphate |