CatLogenzyme kinetics catalog

EC 5.3.3.1: steroid DELTA-isomerase

IsomerasesOpen in Browse

CatLog read 37 records for EC 5.3.3.1 from 6 papers, for 2 organisms. Names in these papers: 3β-hydroxysteroid dehydrogenase/isomerase type 1 (3β-HSD1), 3β-hydroxysteroid dehydrogenase/isomerase type 1, ketosteroid isomerase (ppKSI, Δ5-3-ketosteroid isomerase), type 1 3beta-hydroxysteroid dehydrogenase/isomerase (isomerase activity) and 4 more.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units.

CatLog also holds 121 records for EC 5.3.3.1 that are not yet re-read: from its earlier version, built from the enzyme databases and partly checked against the papers. All records for EC 5.3.3.1 in Browse.

Papers

PaperOrganismsRecords
Structure/function of human type 1 3beta-hydroxysteroid dehydrogenase: An intrasubunit disulfide bond in the Rossmann-fold domain and a Cys residue in the active site are critical for substrate and…Homo sapiens12
Structure/function relationships responsible for the kinetic differences between human type 1 and type 2 3beta-hydroxysteroid dehydrogenase and for the catalysis of the type 1 activityHomo sapiens7
Identification of key amino acids responsible for the substantially higher affinities of human type 1 3beta-hydroxysteroid dehydrogenase/isomerase (3beta-HSD1) for substrates, coenzymes, and…Homo sapiens6
Structure/function relationships responsible for coenzyme specificity and the isomerase activity of human type 1 3 beta-hydroxysteroid dehydrogenase/isomeraseHomo sapiens5
A tale of two isomerases: compact versus extended active sites in ketosteroid isomerase and phosphoglucose isomerasePseudomonas putida5
Contribution of a low-barrier hydrogen bond to catalysis is not significant in ketosteroid isomerasePseudomonas putida2

Records

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)ConditionsPaper
3β-hydroxysteroid dehydrogenase/isomerase type 1Homo sapiensandrost-5-ene-3,17-dione0.8450.027930.3pH 7.4, 27 °CPMID 12205101Open
3β-hydroxysteroid dehydrogenase/isomerase type 1variant H156YHomo sapiensandrost-5-ene-3,17-dione0.9180.027932.8pH 7.4, 27 °CPMID 12205101Open
3β-hydroxysteroid dehydrogenase/isomerase type 1variant K158QHomo sapiensandrost-5-ene-3,17-dione0.1730.02866pH 7.4, 27 °CPMID 12205101Open
3β-hydroxysteroid dehydrogenase/isomerase type 1variant K273QHomo sapiensandrost-5-ene-3,17-dione0.0430.10080.33pH 7.4, 27 °CPMID 12205101Open
3β-hydroxysteroid dehydrogenase/isomerase type 1variant Y154FHomo sapiensandrost-5-ene-3,17-dione0.2070.02887.2pH 7.4, 27 °CPMID 12205101Open
3β-hydroxysteroid dehydrogenase/isomerase type 1variant Y269SHomo sapiensandrost-5-ene-3,17-dione0.0450.09080.5pH 7.4, 27 °CPMID 12205101Open
3β-hydroxysteroid dehydrogenase/isomerase type 1 (3β-HSD1)Homo sapiensandrost-5-ene-3,17-dionewithout β-mercaptoethanol (BME)——30pH 7.4, 27 °CPMID 17624763Open
3β-hydroxysteroid dehydrogenase/isomerase type 1 (3β-HSD1)Homo sapiensandrost-5-ene-3,17-dionewith 10 mM β-mercaptoethanol (BME)0.8950.0059151.7pH 7.4, 27 °CPMID 17624763Open
3β-hydroxysteroid dehydrogenase/isomerase type 1 (3β-HSD1)variant C111AHomo sapiensandrost-5-ene-3,17-dionewithout β-mercaptoethanol (BME)0.4030.03212.7pH 7.4, 27 °CPMID 17624763Open
3β-hydroxysteroid dehydrogenase/isomerase type 1 (3β-HSD1)variant C111AHomo sapiensandrost-5-ene-3,17-dionewith 10 mM β-mercaptoethanol (BME)0.3920.030812.7pH 7.4, 27 °CPMID 17624763Open
3β-hydroxysteroid dehydrogenase/isomerase type 1 (3β-HSD1)variant C111SHomo sapiensandrost-5-ene-3,17-dionewithout β-mercaptoethanol (BME)1.30.078516.5pH 7.4, 27 °CPMID 17624763Open
3β-hydroxysteroid dehydrogenase/isomerase type 1 (3β-HSD1)variant C111SHomo sapiensandrost-5-ene-3,17-dionewith 10 mM β-mercaptoethanol (BME)1.2550.065919pH 7.4, 27 °CPMID 17624763Open
3β-hydroxysteroid dehydrogenase/isomerase type 1 (3β-HSD1)variant C72FHomo sapiensandrost-5-ene-3,17-dionewithout β-mercaptoethanol (BME)1.1280.06218.2pH 7.4, 27 °CPMID 17624763Open
3β-hydroxysteroid dehydrogenase/isomerase type 1 (3β-HSD1)variant C72FHomo sapiensandrost-5-ene-3,17-dionewith 10 mM β-mercaptoethanol (BME)1.0980.06716.3pH 7.4, 27 °CPMID 17624763Open
3β-hydroxysteroid dehydrogenase/isomerase type 1 (3β-HSD1)variant C72SHomo sapiensandrost-5-ene-3,17-dionewithout β-mercaptoethanol (BME)0.0480.01922.5pH 7.4, 27 °CPMID 17624763Open
3β-hydroxysteroid dehydrogenase/isomerase type 1 (3β-HSD1)variant C72SHomo sapiensandrost-5-ene-3,17-dionewith 10 mM β-mercaptoethanol (BME)0.0480.02681.8pH 7.4, 27 °CPMID 17624763Open
3β-hydroxysteroid dehydrogenase/isomerase type 1 (3β-HSD1)variant C83AHomo sapiensandrost-5-ene-3,17-dionewithout β-mercaptoethanol (BME)1.8770.070926.5pH 7.4, 27 °CPMID 17624763Open
3β-hydroxysteroid dehydrogenase/isomerase type 1 (3β-HSD1)variant C83AHomo sapiensandrost-5-ene-3,17-dionewith 10 mM β-mercaptoethanol (BME)1.1580.028141.2pH 7.4, 27 °CPMID 17624763Open
3β-hydroxysteroid dehydrogenase/isomerase type 2Homo sapiensandrost-5-ene-3,17-dione1.370.088415.5pH 7.4, 27 °CPMID 12205101Open
human type 1 3β-hydroxysteroid dehydrogenase/isomerase (3β-HSD1)Homo sapiens5-androstene-3,17-dione0.837—20.8pH 7.4, 27 °CPMID 15797861Open
human type 1 3β-hydroxysteroid dehydrogenase/isomerase (3β-HSD1)variant Q105MHomo sapiens5-androstene-3,17-dione0.580.03317.7pH 7.4, 27 °CPMID 15797861Open
human type 1 3β-hydroxysteroid dehydrogenase/isomerase (3β-HSD1)variant Q240RHomo sapiens5-androstene-3,17-dione0.5550.06238.8pH 7.4, 27 °CPMID 15797861Open
human type 2 3β-hydroxysteroid dehydrogenase/isomerase (3β-HSD2)Homo sapiens5-androstene-3,17-dione1.358—15.3pH 7.4, 27 °CPMID 15797861Open
human type 2 3β-hydroxysteroid dehydrogenase/isomerase (3β-HSD2)variant Q105MHomo sapiens5-androstene-3,17-dione1.1120.080813.7pH 7.4, 27 °CPMID 15797861Open
human type 2 3β-hydroxysteroid dehydrogenase/isomerase (3β-HSD2)variant R240QHomo sapiens5-androstene-3,17-dione0.5730.017532.8pH 7.4, 27 °CPMID 15797861Open
ketosteroid isomerase (ppKSI, Δ5-3-ketosteroid isomerase)Pseudomonas putida5-androstene-3,17-dione3.6 × 10⁴0.0894.1 × 10⁵pH 7, 21 °CPMID 21970785Open
ketosteroid isomerase (ppKSI, Δ5-3-ketosteroid isomerase)variant E39QPseudomonas putida5-androstene-3,17-dione1.9 × 10⁴0.111.7 × 10⁵pH 7, 21 °CPMID 21970785Open
ketosteroid isomerase (ppKSI, Δ5-3-ketosteroid isomerase)variant M105APseudomonas putida5-androstene-3,17-dione2.4 × 10³0.0445.4 × 10⁴pH 7, 21 °CPMID 21970785Open
ketosteroid isomerase (ppKSI, Δ5-3-ketosteroid isomerase)variant M31APseudomonas putida5-androstene-3,17-dione1.8 × 10⁴0.0892 × 10⁵pH 7, 21 °CPMID 21970785Open
ketosteroid isomerase (ppKSI, Δ5-3-ketosteroid isomerase)variant M84APseudomonas putida5-androstene-3,17-dione3.4 × 10⁴0.0814.2 × 10⁵pH 7, 21 °CPMID 21970785Open
type 1 3beta-hydroxysteroid dehydrogenase/isomerase (isomerase activity)Homo sapiens5-androstene-3,17-dionepurified [Table IV]0.5570.026820.8 calc.pH 7.4, 27 °CPMID 12832414Open
type 1 3beta-hydroxysteroid dehydrogenase/isomerase (isomerase activity)Homo sapiens5-androstene-3,17-dionepurified [Table V]—0.0263—pH 7.4, 27 °CPMID 12832414Open
type 1 3beta-hydroxysteroid dehydrogenase/isomerase (isomerase activity)variant D241NHomo sapiens5-androstene-3,17-dione0.4820.023320.7 calc.pH 7.4, 27 °CPMID 12832414Open
type 1 3beta-hydroxysteroid dehydrogenase/isomerase (isomerase activity)variant D265NHomo sapiens5-androstene-3,17-dione0.2950.04836.11 calc.pH 7.4, 27 °CPMID 12832414Open
type 1 3beta-hydroxysteroid dehydrogenase/isomerase (isomerase activity)variant D36A/K37RHomo sapiens5-androstene-3,17-dione0.1770.03045.81 calc.pH 7.4, 27 °CPMID 12832414Open
Δ5-3-ketosteroid isomerase (KSI)variant Y115FPseudomonas putida5-androstene-3,17-dione1.45 × 10⁴0.04173.5 × 10⁵pH 7, 25 °CPMID 25947291Open
Δ5-3-ketosteroid isomerase (KSI)variant Y30F/Y55F/Y115FPseudomonas putida5-androstene-3,17-dione1.22 × 10⁴0.07731.6 × 10⁵pH 7, 25 °CPMID 25947291Open