Values CatLog read from this paper
Structure/function relationships responsible for coenzyme specificity and the isomerase activity of human type 1 3 beta-hydroxysteroid dehydrogenase/isomerase
PMID 12832414doi:10.1074/jbc.m304752200Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 13 records (25 values) from this paper: type 1 3beta-hydroxysteroid dehydrogenase/isomerase (3beta-HSD activity), type 1 3beta-hydroxysteroid dehydrogenase/isomerase (isomerase activity) from Homo sapiens.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 12 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| type 1 3beta-hydroxysteroid dehydrogenase/isomerase (3beta-HSD activity) | Homo sapiens | dehydroepiandrosterone | 0.055 | 0.0037 | 15 calc. | pH 7.4, 27 °C | Open record: type 1 3beta-hydroxysteroid dehydrogenase/isomerase (3beta-HSD activity), dehydroepiandrosterone |
| type 1 3beta-hydroxysteroid dehydrogenase/isomerase (3beta-HSD activity) | Homo sapiens | NAD+ | 0.065 | 0.0341 | 1.9 calc. | pH 7.4, 27 °C | Open record: type 1 3beta-hydroxysteroid dehydrogenase/isomerase (3beta-HSD activity), NAD+ |
| type 1 3beta-hydroxysteroid dehydrogenase/isomerase (3beta-HSD activity)variant D241N | Homo sapiens | dehydroepiandrosterone | 0.022 | 0.0118 | 1.8 calc. | pH 7.4, 27 °C | Open record: type 1 3beta-hydroxysteroid dehydrogenase/isomerase (3beta-HSD activity) variant D241N, dehydroepiandrosterone |
| type 1 3beta-hydroxysteroid dehydrogenase/isomerase (3beta-HSD activity)variant D241N | Homo sapiens | NAD+ | 0.017 | 0.0223 | 0.75 calc. | pH 7.4, 27 °C | Open record: type 1 3beta-hydroxysteroid dehydrogenase/isomerase (3beta-HSD activity) variant D241N, NAD+ |
| type 1 3beta-hydroxysteroid dehydrogenase/isomerase (3beta-HSD activity)variant D265N | Homo sapiens | dehydroepiandrosterone | 0.0067 | 0.0106 | 0.63 calc. | pH 7.4, 27 °C | Open record: type 1 3beta-hydroxysteroid dehydrogenase/isomerase (3beta-HSD activity) variant D265N, dehydroepiandrosterone |
| type 1 3beta-hydroxysteroid dehydrogenase/isomerase (3beta-HSD activity)variant D265N | Homo sapiens | NAD+ | 0.0067 | 0.0337 | 0.20 calc. | pH 7.4, 27 °C | Open record: type 1 3beta-hydroxysteroid dehydrogenase/isomerase (3beta-HSD activity) variant D265N, NAD+ |
| type 1 3beta-hydroxysteroid dehydrogenase/isomerase (3beta-HSD activity)variant D36A/K37R | Homo sapiens | dehydroepiandrosterone | 0.018 | 0.0048 | 3.8 calc. | pH 7.4, 27 °C | Open record: type 1 3beta-hydroxysteroid dehydrogenase/isomerase (3beta-HSD activity) variant D36A/K37R, dehydroepiandrosterone |
| type 1 3beta-hydroxysteroid dehydrogenase/isomerase (3beta-HSD activity)variant D36A/K37R | Homo sapiens | NADP+ | 0.022 | 0.0206 | 1.1 calc. | pH 7.4, 27 °C | Open record: type 1 3beta-hydroxysteroid dehydrogenase/isomerase (3beta-HSD activity) variant D36A/K37R, NADP+ |
| type 1 3beta-hydroxysteroid dehydrogenase/isomerase (isomerase activity) | Homo sapiens | 5-androstene-3,17-dionepurified [Table IV] | 0.557 | 0.0268 | 20.8 calc. | pH 7.4, 27 °C | Open record: type 1 3beta-hydroxysteroid dehydrogenase/isomerase (isomerase activity), 5-androstene-3,17-dione |
| type 1 3beta-hydroxysteroid dehydrogenase/isomerase (isomerase activity) | Homo sapiens | 5-androstene-3,17-dionepurified [Table V] | — | 0.0263 | — | pH 7.4, 27 °C | Open record: type 1 3beta-hydroxysteroid dehydrogenase/isomerase (isomerase activity), 5-androstene-3,17-dione |
| type 1 3beta-hydroxysteroid dehydrogenase/isomerase (isomerase activity)variant D241N | Homo sapiens | 5-androstene-3,17-dione | 0.482 | 0.0233 | 20.7 calc. | pH 7.4, 27 °C | Open record: type 1 3beta-hydroxysteroid dehydrogenase/isomerase (isomerase activity) variant D241N, 5-androstene-3,17-dione |
| type 1 3beta-hydroxysteroid dehydrogenase/isomerase (isomerase activity)variant D265N | Homo sapiens | 5-androstene-3,17-dione | 0.295 | 0.0483 | 6.11 calc. | pH 7.4, 27 °C | Open record: type 1 3beta-hydroxysteroid dehydrogenase/isomerase (isomerase activity) variant D265N, 5-androstene-3,17-dione |
| type 1 3beta-hydroxysteroid dehydrogenase/isomerase (isomerase activity)variant D36A/K37R | Homo sapiens | 5-androstene-3,17-dione | 0.177 | 0.0304 | 5.81 calc. | pH 7.4, 27 °C | Open record: type 1 3beta-hydroxysteroid dehydrogenase/isomerase (isomerase activity) variant D36A/K37R, 5-androstene-3,17-dione |