Values CatLog read from this paper

Structure/function of human type 1 3beta-hydroxysteroid dehydrogenase: An intrasubunit disulfide bond in the Rossmann-fold domain and a Cys residue in the active site are critical for substrate and…

PMID 17624763doi:10.1016/j.jsbmb.2007.02.003Open in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 34 records (100 values) from this paper: 3β-hydroxysteroid dehydrogenase/isomerase type 1 (3β-HSD1) from Homo sapiens.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.

EC 1.1.1.145, EC 5.3.3.1

Records read from this paper
Enzyme	Organism	Substrate	k_cat (s⁻¹)	K_m (mM)	k_cat/K_m (mM⁻¹ s⁻¹)	Conditions	Open record
3β-hydroxysteroid dehydrogenase/isomerase type 1 (3β-HSD1)	Homo sapiens	androst-5-ene-3,17-dionewithout β-mercaptoethanol (BME)	—	—	30	pH 7.4, 27 °C	Open record: 3β-hydroxysteroid dehydrogenase/isomerase type 1 (3β-HSD1), androst-5-ene-3,17-dione
3β-hydroxysteroid dehydrogenase/isomerase type 1 (3β-HSD1)	Homo sapiens	androst-5-ene-3,17-dionewith 10 mM β-mercaptoethanol (BME)	0.895	0.0059	151.7	pH 7.4, 27 °C	Open record: 3β-hydroxysteroid dehydrogenase/isomerase type 1 (3β-HSD1), androst-5-ene-3,17-dione
3β-hydroxysteroid dehydrogenase/isomerase type 1 (3β-HSD1)	Homo sapiens	dehydroepiandrosterone	0.048	7 × 10⁻⁴	69	pH 7.4, 27 °C	Open record: 3β-hydroxysteroid dehydrogenase/isomerase type 1 (3β-HSD1), dehydroepiandrosterone
3β-hydroxysteroid dehydrogenase/isomerase type 1 (3β-HSD1)	Homo sapiens	nicotinamide adenine dinucleotide (oxidized)	0.048	0.012	4	pH 7.4, 27 °C	Open record: 3β-hydroxysteroid dehydrogenase/isomerase type 1 (3β-HSD1), nicotinamide adenine dinucleotide (oxidized)
3β-hydroxysteroid dehydrogenase/isomerase type 1 (3β-HSD1)variant C111A	Homo sapiens	androst-5-ene-3,17-dionewithout β-mercaptoethanol (BME)	0.403	0.032	12.7	pH 7.4, 27 °C	Open record: 3β-hydroxysteroid dehydrogenase/isomerase type 1 (3β-HSD1) variant C111A, androst-5-ene-3,17-dione
3β-hydroxysteroid dehydrogenase/isomerase type 1 (3β-HSD1)variant C111A	Homo sapiens	androst-5-ene-3,17-dionewith 10 mM β-mercaptoethanol (BME)	0.392	0.0308	12.7	pH 7.4, 27 °C	Open record: 3β-hydroxysteroid dehydrogenase/isomerase type 1 (3β-HSD1) variant C111A, androst-5-ene-3,17-dione
3β-hydroxysteroid dehydrogenase/isomerase type 1 (3β-HSD1)variant C111A	Homo sapiens	dehydroepiandrosteronewithout β-mercaptoethanol (BME)	0.032	0.0268	1.2	pH 7.4, 27 °C	Open record: 3β-hydroxysteroid dehydrogenase/isomerase type 1 (3β-HSD1) variant C111A, dehydroepiandrosterone
3β-hydroxysteroid dehydrogenase/isomerase type 1 (3β-HSD1)variant C111A	Homo sapiens	dehydroepiandrosteronewith 10 mM β-mercaptoethanol (BME)	0.03	0.0237	1.3	pH 7.4, 27 °C	Open record: 3β-hydroxysteroid dehydrogenase/isomerase type 1 (3β-HSD1) variant C111A, dehydroepiandrosterone
3β-hydroxysteroid dehydrogenase/isomerase type 1 (3β-HSD1)variant C111A	Homo sapiens	nicotinamide adenine dinucleotide (oxidized)without β-mercaptoethanol (BME)	0.035	0.0255	1.3	pH 7.4, 27 °C	Open record: 3β-hydroxysteroid dehydrogenase/isomerase type 1 (3β-HSD1) variant C111A, nicotinamide adenine dinucleotide (oxidized)
3β-hydroxysteroid dehydrogenase/isomerase type 1 (3β-HSD1)variant C111A	Homo sapiens	nicotinamide adenine dinucleotide (oxidized)with 10 mM β-mercaptoethanol (BME)	0.032	0.0227	1.3	pH 7.4, 27 °C	Open record: 3β-hydroxysteroid dehydrogenase/isomerase type 1 (3β-HSD1) variant C111A, nicotinamide adenine dinucleotide (oxidized)
3β-hydroxysteroid dehydrogenase/isomerase type 1 (3β-HSD1)variant C111S	Homo sapiens	androst-5-ene-3,17-dionewithout β-mercaptoethanol (BME)	1.3	0.0785	16.5	pH 7.4, 27 °C	Open record: 3β-hydroxysteroid dehydrogenase/isomerase type 1 (3β-HSD1) variant C111S, androst-5-ene-3,17-dione
3β-hydroxysteroid dehydrogenase/isomerase type 1 (3β-HSD1)variant C111S	Homo sapiens	androst-5-ene-3,17-dionewith 10 mM β-mercaptoethanol (BME)	1.255	0.0659	19	pH 7.4, 27 °C	Open record: 3β-hydroxysteroid dehydrogenase/isomerase type 1 (3β-HSD1) variant C111S, androst-5-ene-3,17-dione
3β-hydroxysteroid dehydrogenase/isomerase type 1 (3β-HSD1)variant C111S	Homo sapiens	dehydroepiandrosteronewithout β-mercaptoethanol (BME)	0.087	0.0181	4.8	pH 7.4, 27 °C	Open record: 3β-hydroxysteroid dehydrogenase/isomerase type 1 (3β-HSD1) variant C111S, dehydroepiandrosterone
3β-hydroxysteroid dehydrogenase/isomerase type 1 (3β-HSD1)variant C111S	Homo sapiens	dehydroepiandrosteronewith 10 mM β-mercaptoethanol (BME)	0.087	0.0168	5.2	pH 7.4, 27 °C	Open record: 3β-hydroxysteroid dehydrogenase/isomerase type 1 (3β-HSD1) variant C111S, dehydroepiandrosterone
3β-hydroxysteroid dehydrogenase/isomerase type 1 (3β-HSD1)variant C111S	Homo sapiens	nicotinamide adenine dinucleotide (oxidized)without β-mercaptoethanol (BME)	0.087	0.019	4.5	pH 7.4, 27 °C	Open record: 3β-hydroxysteroid dehydrogenase/isomerase type 1 (3β-HSD1) variant C111S, nicotinamide adenine dinucleotide (oxidized)
3β-hydroxysteroid dehydrogenase/isomerase type 1 (3β-HSD1)variant C111S	Homo sapiens	nicotinamide adenine dinucleotide (oxidized)with 10 mM β-mercaptoethanol (BME)	0.087	0.0166	5.2	pH 7.4, 27 °C	Open record: 3β-hydroxysteroid dehydrogenase/isomerase type 1 (3β-HSD1) variant C111S, nicotinamide adenine dinucleotide (oxidized)
3β-hydroxysteroid dehydrogenase/isomerase type 1 (3β-HSD1)variant C72F	Homo sapiens	androst-5-ene-3,17-dionewithout β-mercaptoethanol (BME)	1.128	0.062	18.2	pH 7.4, 27 °C	Open record: 3β-hydroxysteroid dehydrogenase/isomerase type 1 (3β-HSD1) variant C72F, androst-5-ene-3,17-dione
3β-hydroxysteroid dehydrogenase/isomerase type 1 (3β-HSD1)variant C72F	Homo sapiens	androst-5-ene-3,17-dionewith 10 mM β-mercaptoethanol (BME)	1.098	0.067	16.3	pH 7.4, 27 °C	Open record: 3β-hydroxysteroid dehydrogenase/isomerase type 1 (3β-HSD1) variant C72F, androst-5-ene-3,17-dione
3β-hydroxysteroid dehydrogenase/isomerase type 1 (3β-HSD1)variant C72F	Homo sapiens	dehydroepiandrosteronewithout β-mercaptoethanol (BME)	0.053	0.0127	4.2	pH 7.4, 27 °C	Open record: 3β-hydroxysteroid dehydrogenase/isomerase type 1 (3β-HSD1) variant C72F, dehydroepiandrosterone
3β-hydroxysteroid dehydrogenase/isomerase type 1 (3β-HSD1)variant C72F	Homo sapiens	dehydroepiandrosteronewith 10 mM β-mercaptoethanol (BME)	0.05	0.0115	4.3	pH 7.4, 27 °C	Open record: 3β-hydroxysteroid dehydrogenase/isomerase type 1 (3β-HSD1) variant C72F, dehydroepiandrosterone
3β-hydroxysteroid dehydrogenase/isomerase type 1 (3β-HSD1)variant C72F	Homo sapiens	nicotinamide adenine dinucleotide (oxidized)without β-mercaptoethanol (BME)	0.043	0.0468	1	pH 7.4, 27 °C	Open record: 3β-hydroxysteroid dehydrogenase/isomerase type 1 (3β-HSD1) variant C72F, nicotinamide adenine dinucleotide (oxidized)
3β-hydroxysteroid dehydrogenase/isomerase type 1 (3β-HSD1)variant C72F	Homo sapiens	nicotinamide adenine dinucleotide (oxidized)with 10 mM β-mercaptoethanol (BME)	0.042	0.0476	0.83	pH 7.4, 27 °C	Open record: 3β-hydroxysteroid dehydrogenase/isomerase type 1 (3β-HSD1) variant C72F, nicotinamide adenine dinucleotide (oxidized)
3β-hydroxysteroid dehydrogenase/isomerase type 1 (3β-HSD1)variant C72S	Homo sapiens	androst-5-ene-3,17-dionewithout β-mercaptoethanol (BME)	0.048	0.0192	2.5	pH 7.4, 27 °C	Open record: 3β-hydroxysteroid dehydrogenase/isomerase type 1 (3β-HSD1) variant C72S, androst-5-ene-3,17-dione
3β-hydroxysteroid dehydrogenase/isomerase type 1 (3β-HSD1)variant C72S	Homo sapiens	androst-5-ene-3,17-dionewith 10 mM β-mercaptoethanol (BME)	0.048	0.0268	1.8	pH 7.4, 27 °C	Open record: 3β-hydroxysteroid dehydrogenase/isomerase type 1 (3β-HSD1) variant C72S, androst-5-ene-3,17-dione
3β-hydroxysteroid dehydrogenase/isomerase type 1 (3β-HSD1)variant C72S	Homo sapiens	dehydroepiandrosteronewithout β-mercaptoethanol (BME)	0.02	0.0028	7.2	pH 7.4, 27 °C	Open record: 3β-hydroxysteroid dehydrogenase/isomerase type 1 (3β-HSD1) variant C72S, dehydroepiandrosterone
3β-hydroxysteroid dehydrogenase/isomerase type 1 (3β-HSD1)variant C72S	Homo sapiens	dehydroepiandrosteronewith 10 mM β-mercaptoethanol (BME)	0.022	0.0044	3.8	pH 7.4, 27 °C	Open record: 3β-hydroxysteroid dehydrogenase/isomerase type 1 (3β-HSD1) variant C72S, dehydroepiandrosterone
3β-hydroxysteroid dehydrogenase/isomerase type 1 (3β-HSD1)variant C72S	Homo sapiens	nicotinamide adenine dinucleotide (oxidized)without β-mercaptoethanol (BME)	0.022	0.0153	1.3	pH 7.4, 27 °C	Open record: 3β-hydroxysteroid dehydrogenase/isomerase type 1 (3β-HSD1) variant C72S, nicotinamide adenine dinucleotide (oxidized)
3β-hydroxysteroid dehydrogenase/isomerase type 1 (3β-HSD1)variant C72S	Homo sapiens	nicotinamide adenine dinucleotide (oxidized)with 10 mM β-mercaptoethanol (BME)	0.02	0.0194	1	pH 7.4, 27 °C	Open record: 3β-hydroxysteroid dehydrogenase/isomerase type 1 (3β-HSD1) variant C72S, nicotinamide adenine dinucleotide (oxidized)
3β-hydroxysteroid dehydrogenase/isomerase type 1 (3β-HSD1)variant C83A	Homo sapiens	androst-5-ene-3,17-dionewithout β-mercaptoethanol (BME)	1.877	0.0709	26.5	pH 7.4, 27 °C	Open record: 3β-hydroxysteroid dehydrogenase/isomerase type 1 (3β-HSD1) variant C83A, androst-5-ene-3,17-dione
3β-hydroxysteroid dehydrogenase/isomerase type 1 (3β-HSD1)variant C83A	Homo sapiens	androst-5-ene-3,17-dionewith 10 mM β-mercaptoethanol (BME)	1.158	0.0281	41.2	pH 7.4, 27 °C	Open record: 3β-hydroxysteroid dehydrogenase/isomerase type 1 (3β-HSD1) variant C83A, androst-5-ene-3,17-dione
3β-hydroxysteroid dehydrogenase/isomerase type 1 (3β-HSD1)variant C83A	Homo sapiens	dehydroepiandrosteronewithout β-mercaptoethanol (BME)	0.06	0.0171	3.5	pH 7.4, 27 °C	Open record: 3β-hydroxysteroid dehydrogenase/isomerase type 1 (3β-HSD1) variant C83A, dehydroepiandrosterone
3β-hydroxysteroid dehydrogenase/isomerase type 1 (3β-HSD1)variant C83A	Homo sapiens	dehydroepiandrosteronewith 10 mM β-mercaptoethanol (BME)	0.047	0.0075	2.7	pH 7.4, 27 °C	Open record: 3β-hydroxysteroid dehydrogenase/isomerase type 1 (3β-HSD1) variant C83A, dehydroepiandrosterone
3β-hydroxysteroid dehydrogenase/isomerase type 1 (3β-HSD1)variant C83A	Homo sapiens	nicotinamide adenine dinucleotide (oxidized)without β-mercaptoethanol (BME)	0.063	0.0732	0.83	pH 7.4, 27 °C	Open record: 3β-hydroxysteroid dehydrogenase/isomerase type 1 (3β-HSD1) variant C83A, nicotinamide adenine dinucleotide (oxidized)
3β-hydroxysteroid dehydrogenase/isomerase type 1 (3β-HSD1)variant C83A	Homo sapiens	nicotinamide adenine dinucleotide (oxidized)with 10 mM β-mercaptoethanol (BME)	0.04	0.0299	1.3	pH 7.4, 27 °C	Open record: 3β-hydroxysteroid dehydrogenase/isomerase type 1 (3β-HSD1) variant C83A, nicotinamide adenine dinucleotide (oxidized)