CatLogenzyme kinetics catalog

Values CatLog read from this paper

Structure/function relationships responsible for the kinetic differences between human type 1 and type 2 3beta-hydroxysteroid dehydrogenase and for the catalysis of the type 1 activity

PMID 12205101doi:10.1074/jbc.m208537200Open in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 19 records (45 values) from this paper: 3β-hydroxysteroid dehydrogenase/isomerase type 1, 3β-hydroxysteroid dehydrogenase/isomerase type 2 from Homo sapiens.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.

EC 1.1.1.145, EC 5.3.3.1

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)Conditions
3β-hydroxysteroid dehydrogenase/isomerase type 1Homo sapiens17α-hydroxypregnenolone—0.0035—pH 7.4, 27 °COpen
3β-hydroxysteroid dehydrogenase/isomerase type 1Homo sapiensandrost-5-ene-3,17-dione0.8450.027930.3pH 7.4, 27 °COpen
3β-hydroxysteroid dehydrogenase/isomerase type 1Homo sapiensdehydroepiandrosterone0.0620.003716.7pH 7.4, 27 °COpen
3β-hydroxysteroid dehydrogenase/isomerase type 1Homo sapiensNAD+0.0650.03411.8pH 7.4, 27 °COpen
3β-hydroxysteroid dehydrogenase/isomerase type 1Homo sapienspregnenolone—0.0028—pH 7.4, 27 °COpen
3β-hydroxysteroid dehydrogenase/isomerase type 1variant H156YHomo sapiens17α-hydroxypregnenolone—0.0134—pH 7.4, 27 °COpen
3β-hydroxysteroid dehydrogenase/isomerase type 1variant H156YHomo sapiensandrost-5-ene-3,17-dione0.9180.027932.8pH 7.4, 27 °COpen
3β-hydroxysteroid dehydrogenase/isomerase type 1variant H156YHomo sapiensdehydroepiandrosterone0.120.04242.8pH 7.4, 27 °COpen
3β-hydroxysteroid dehydrogenase/isomerase type 1variant H156YHomo sapiensNAD+0.0770.0253pH 7.4, 27 °COpen
3β-hydroxysteroid dehydrogenase/isomerase type 1variant H156YHomo sapienspregnenolone—0.0437—pH 7.4, 27 °COpen
3β-hydroxysteroid dehydrogenase/isomerase type 1variant K158QHomo sapiensandrost-5-ene-3,17-dione0.1730.02866pH 7.4, 27 °COpen
3β-hydroxysteroid dehydrogenase/isomerase type 1variant K273QHomo sapiensandrost-5-ene-3,17-dione0.0430.10080.33pH 7.4, 27 °COpen
3β-hydroxysteroid dehydrogenase/isomerase type 1variant Y154FHomo sapiensandrost-5-ene-3,17-dione0.2070.02887.2pH 7.4, 27 °COpen
3β-hydroxysteroid dehydrogenase/isomerase type 1variant Y269SHomo sapiensandrost-5-ene-3,17-dione0.0450.09080.5pH 7.4, 27 °COpen
3β-hydroxysteroid dehydrogenase/isomerase type 2Homo sapiens17α-hydroxypregnenolone—0.0178—pH 7.4, 27 °COpen
3β-hydroxysteroid dehydrogenase/isomerase type 2Homo sapiensandrost-5-ene-3,17-dione1.370.088415.5pH 7.4, 27 °COpen
3β-hydroxysteroid dehydrogenase/isomerase type 2Homo sapiensdehydroepiandrosterone0.1150.04732.3pH 7.4, 27 °COpen
3β-hydroxysteroid dehydrogenase/isomerase type 2Homo sapiensNAD+0.1180.08631.3pH 7.4, 27 °COpen
3β-hydroxysteroid dehydrogenase/isomerase type 2Homo sapienspregnenolone—0.0495—pH 7.4, 27 °COpen