Values CatLog read from this paper
Contribution of a low-barrier hydrogen bond to catalysis is not significant in ketosteroid isomerase
PMID 25947291doi:10.14348/molcells.2015.2266Open in Browse
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CatLog read 2 records (6 values) from this paper: Δ5-3-ketosteroid isomerase (KSI) from Pseudomonas putida.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| Δ5-3-ketosteroid isomerase (KSI)variant Y115F | Pseudomonas putida | 5-androstene-3,17-dione | 1.45 × 10⁴ | 0.0417 | 3.5 × 10⁵ | pH 7, 25 °C | Open record: Δ5-3-ketosteroid isomerase (KSI) variant Y115F, 5-androstene-3,17-dione |
| Δ5-3-ketosteroid isomerase (KSI)variant Y30F/Y55F/Y115F | Pseudomonas putida | 5-androstene-3,17-dione | 1.22 × 10⁴ | 0.0773 | 1.6 × 10⁵ | pH 7, 25 °C | Open record: Δ5-3-ketosteroid isomerase (KSI) variant Y30F/Y55F/Y115F, 5-androstene-3,17-dione |