Values CatLog read from this paper
Substrate recognition by β-ketoacyl-ACP synthases
PMID 22017312doi:10.1021/bi201199xOpen in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 26 records (77 values) from this paper: β-ketoacyl-ACP synthase I (ecFabB, KASI), β-ketoacyl-ACP synthase II (ecFabF, KASII), β-ketoacyl-ACP synthase I (KasA, KASI) from Escherichia coli, Mycobacterium tuberculosis.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 1 kcat/Km value marked “calc.” from kcat and Km; no printed kcat/Km was read for that record.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| β-ketoacyl-ACP synthase I (ecFabB, KASI) | Escherichia coli | lauroyl-acyl carrier protein (Escherichia coli ACP)10 μM malonyl-ecACP (footnote b); cosubstrate malonyl-ecACP | 0.057 | 0.0032 | 18 | pH 6.5, 25 °C | Open record: β-ketoacyl-ACP synthase I (ecFabB, KASI), lauroyl-acyl carrier protein (Escherichia coli ACP) |
| β-ketoacyl-ACP synthase I (ecFabB, KASI) | Escherichia coli | lauroyl-acyl carrier protein (Escherichia coli ACP)200 μM malonyl-CoA (footnote c); cosubstrate malonyl-CoA | 0.033 | 0.0039 | 8.3 | pH 6.5, 25 °C | Open record: β-ketoacyl-ACP synthase I (ecFabB, KASI), lauroyl-acyl carrier protein (Escherichia coli ACP) |
| β-ketoacyl-ACP synthase I (ecFabB, KASI) | Escherichia coli | lauroyl-CoA | 0.073 | 0.0586 | 1.27 | pH 6.5, 25 °C | Open record: β-ketoacyl-ACP synthase I (ecFabB, KASI), lauroyl-CoA |
| β-ketoacyl-ACP synthase I (ecFabB, KASI) | Escherichia coli | malonyl-acyl carrier protein (Escherichia coli ACP) | 0.11 | 0.0115 | 10 | pH 6.5, 25 °C | Open record: β-ketoacyl-ACP synthase I (ecFabB, KASI), malonyl-acyl carrier protein (Escherichia coli ACP) |
| β-ketoacyl-ACP synthase I (ecFabB, KASI) | Escherichia coli | malonyl-CoA | 0.118 | 0.153 | 0.78 | pH 6.5, 25 °C | Open record: β-ketoacyl-ACP synthase I (ecFabB, KASI), malonyl-CoA |
| β-ketoacyl-ACP synthase I (ecFabB, KASI) | Escherichia coli | malonyl-phosphopantetheine-14-mer | 0.04 | 0.0158 | 3 | pH 6.5, 25 °C | Open record: β-ketoacyl-ACP synthase I (ecFabB, KASI), malonyl-phosphopantetheine-14-mer |
| β-ketoacyl-ACP synthase I (ecFabB, KASI) | Escherichia coli | malonyl-phosphopantetheine-16-mer | 0.108 | 0.029 | 3.8 | pH 6.5, 25 °C | Open record: β-ketoacyl-ACP synthase I (ecFabB, KASI), malonyl-phosphopantetheine-16-mer |
| β-ketoacyl-ACP synthase I (ecFabB, KASI) | Escherichia coli | malonyl-phosphopantetheine-8-mer | 0.018 | 0.0142 | 1.3 | pH 6.5, 25 °C | Open record: β-ketoacyl-ACP synthase I (ecFabB, KASI), malonyl-phosphopantetheine-8-mer |
| β-ketoacyl-ACP synthase I (ecFabB, KASI)variant K151Q | Escherichia coli | lauroyl-acyl carrier protein (Escherichia coli ACP) | 0.025 | 0.0067 | 3.7 | pH 6.5, 25 °C | Open record: β-ketoacyl-ACP synthase I (ecFabB, KASI) variant K151Q, lauroyl-acyl carrier protein (Escherichia coli ACP) |
| β-ketoacyl-ACP synthase I (ecFabB, KASI)variant K63Q | Escherichia coli | lauroyl-acyl carrier protein (Escherichia coli ACP) | 0.083 | 0.06 | 1.2 | pH 6.5, 25 °C | Open record: β-ketoacyl-ACP synthase I (ecFabB, KASI) variant K63Q, lauroyl-acyl carrier protein (Escherichia coli ACP) |
| β-ketoacyl-ACP synthase I (ecFabB, KASI)variant R62Q | Escherichia coli | lauroyl-acyl carrier protein (Escherichia coli ACP) | 0.077 | 0.05 | 1.5 | pH 6.5, 25 °C | Open record: β-ketoacyl-ACP synthase I (ecFabB, KASI) variant R62Q, lauroyl-acyl carrier protein (Escherichia coli ACP) |
| β-ketoacyl-ACP synthase I (ecFabB, KASI)variant R66Q | Escherichia coli | lauroyl-acyl carrier protein (Escherichia coli ACP) | 0.053 | 0.056 | 0.83 | pH 6.5, 25 °C | Open record: β-ketoacyl-ACP synthase I (ecFabB, KASI) variant R66Q, lauroyl-acyl carrier protein (Escherichia coli ACP) |
| β-ketoacyl-ACP synthase I (KasA, KASI) | Mycobacterium tuberculosis | malonyl-acyl carrier protein AcpM (Mycobacterium tuberculosis) | 0.47 | 0.0058 | 87 | pH 8.5, 25 °C | Open record: β-ketoacyl-ACP synthase I (KasA, KASI), malonyl-acyl carrier protein AcpM (Mycobacterium tuberculosis) |
| β-ketoacyl-ACP synthase I (KasA, KASI) | Mycobacterium tuberculosis | malonyl-CoA | 0.35 | 0.009 | 38 | pH 8.5, 25 °C | Open record: β-ketoacyl-ACP synthase I (KasA, KASI), malonyl-CoA |
| β-ketoacyl-ACP synthase I (KasA, KASI) | Mycobacterium tuberculosis | malonyl-phosphopantetheine-14-mer | 0.38 | 0.0086 | 48 | pH 8.5, 25 °C | Open record: β-ketoacyl-ACP synthase I (KasA, KASI), malonyl-phosphopantetheine-14-mer |
| β-ketoacyl-ACP synthase I (KasA, KASI) | Mycobacterium tuberculosis | malonyl-phosphopantetheine-16-mer | 0.3 | 0.0051 | 60 | pH 8.5, 25 °C | Open record: β-ketoacyl-ACP synthase I (KasA, KASI), malonyl-phosphopantetheine-16-mer |
| β-ketoacyl-ACP synthase I (KasA, KASI) | Mycobacterium tuberculosis | malonyl-phosphopantetheine-8-mer | 0.35 | 0.0047 | 75 | pH 8.5, 25 °C | Open record: β-ketoacyl-ACP synthase I (KasA, KASI), malonyl-phosphopantetheine-8-mer |
| β-ketoacyl-ACP synthase I (KasA, KASI) | Mycobacterium tuberculosis | palmitoyl-CoA | 0.083 | 4 × 10⁻⁴ | 210 calc. | pH 8.5, 25 °C | Open record: β-ketoacyl-ACP synthase I (KasA, KASI), palmitoyl-CoA |
| β-ketoacyl-ACP synthase II (ecFabF, KASII) | Escherichia coli | lauroyl-CoA | 0.047 | 0.0537 | 1 | pH 6.5, 25 °C | Open record: β-ketoacyl-ACP synthase II (ecFabF, KASII), lauroyl-CoA |
| β-ketoacyl-ACP synthase II (ecFabF, KASII) | Escherichia coli | malonyl-acyl carrier protein (Escherichia coli ACP) | 0.029 | 0.0082 | 3.8 | pH 6.5, 25 °C | Open record: β-ketoacyl-ACP synthase II (ecFabF, KASII), malonyl-acyl carrier protein (Escherichia coli ACP) |
| β-ketoacyl-ACP synthase II (ecFabF, KASII) | Escherichia coli | malonyl-CoA | 0.042 | 0.51 | 0.083 | pH 6.5, 25 °C | Open record: β-ketoacyl-ACP synthase II (ecFabF, KASII), malonyl-CoA |
| β-ketoacyl-ACP synthase II (ecFabF, KASII) | Escherichia coli | malonyl-phosphopantetheine | 0.022 | 0.59 | 0.037 | pH 6.5, 25 °C | Open record: β-ketoacyl-ACP synthase II (ecFabF, KASII), malonyl-phosphopantetheine |
| β-ketoacyl-ACP synthase II (ecFabF, KASII) | Escherichia coli | malonyl-phosphopantetheine-14-mer | 0.023 | 0.0061 | 4.2 | pH 6.5, 25 °C | Open record: β-ketoacyl-ACP synthase II (ecFabF, KASII), malonyl-phosphopantetheine-14-mer |
| β-ketoacyl-ACP synthase II (ecFabF, KASII) | Escherichia coli | malonyl-phosphopantetheine-16-mer | 0.0223 | 0.0062 | 3.8 | pH 6.5, 25 °C | Open record: β-ketoacyl-ACP synthase II (ecFabF, KASII), malonyl-phosphopantetheine-16-mer |
| β-ketoacyl-ACP synthase II (ecFabF, KASII) | Escherichia coli | malonyl-phosphopantetheine-8-mer | 0.018 | 0.0237 | 0.83 | pH 6.5, 25 °C | Open record: β-ketoacyl-ACP synthase II (ecFabF, KASII), malonyl-phosphopantetheine-8-mer |
| β-ketoacyl-ACP synthase II (ecFabF, KASII)variant R206G | Escherichia coli | malonyl-CoA | 0.0213 | 0.0283 | 0.83 | pH 6.5, 25 °C | Open record: β-ketoacyl-ACP synthase II (ecFabF, KASII) variant R206G, malonyl-CoA |