Values CatLog read from this paper
Structural and kinetic isotope effect studies of nicotinamidase (Pnc1) from Saccharomyces cerevisiae
PMID 22229411doi:10.1021/bi2015508Open in Browse
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CatLog read 17 records (27 values) from this paper: nicotinamidase (Pnc1) from Saccharomyces cerevisiae.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| nicotinamidase (Pnc1) | Saccharomyces cerevisiae | 1-methylnicotinamide | — | — | 0.0086 | pH 7.5, 25 °C | Open record: nicotinamidase (Pnc1), 1-methylnicotinamide |
| nicotinamidase (Pnc1) | Saccharomyces cerevisiae | 5-methylnicotinamide | 1.75 | 0.061 | 29 | pH 7.5, 25 °C | Open record: nicotinamidase (Pnc1), 5-methylnicotinamide |
| nicotinamidase (Pnc1) | Saccharomyces cerevisiae | benzamide | 0.0088 | 0.025 | 0.35 | pH 7.5, 25 °C | Open record: nicotinamidase (Pnc1), benzamide |
| nicotinamidase (Pnc1) | Saccharomyces cerevisiae | nicotinamide | 0.69 | 0.0096 | 72 | pH 7.5, 25 °C | Open record: nicotinamidase (Pnc1), nicotinamide |
| nicotinamidase (Pnc1) | Saccharomyces cerevisiae | nicotinamidelow-pH | 0.45 | — | — | 25 °C | Open record: nicotinamidase (Pnc1), nicotinamide |
| nicotinamidase (Pnc1) | Saccharomyces cerevisiae | nicotinamidehigh-pH | 1.87 | — | — | 25 °C | Open record: nicotinamidase (Pnc1), nicotinamide |
| nicotinamidase (Pnc1) | Saccharomyces cerevisiae | pyrazinamide | 2.56 | 0.157 | 16 | pH 7.5, 25 °C | Open record: nicotinamidase (Pnc1), pyrazinamide |
| nicotinamidase (Pnc1) | Saccharomyces cerevisiae | thionicotinamide | — | — | 0.05 | pH 7.5, 25 °C | Open record: nicotinamidase (Pnc1), thionicotinamide |
| nicotinamidase (Pnc1)variant D51A | Saccharomyces cerevisiae | nicotinamide | 0.02 | — | — | pH 7.5, 25 °C | Open record: nicotinamidase (Pnc1) variant D51A, nicotinamide |
| nicotinamidase (Pnc1)variant D51N | Saccharomyces cerevisiae | nicotinamide | 0.064 | — | — | pH 7.5, 25 °C | Open record: nicotinamidase (Pnc1) variant D51N, nicotinamide |
| nicotinamidase (Pnc1)variant D8A | Saccharomyces cerevisiae | nicotinamide | 9 × 10⁻⁴ | — | — | pH 7.5, 25 °C | Open record: nicotinamidase (Pnc1) variant D8A, nicotinamide |
| nicotinamidase (Pnc1)variant D8E | Saccharomyces cerevisiae | nicotinamide | 0.007 | — | — | pH 7.5, 25 °C | Open record: nicotinamidase (Pnc1) variant D8E, nicotinamide |
| nicotinamidase (Pnc1)variant D8N | Saccharomyces cerevisiae | nicotinamide | 6 × 10⁻⁴ | — | — | pH 7.5, 25 °C | Open record: nicotinamidase (Pnc1) variant D8N, nicotinamide |
| nicotinamidase (Pnc1)variant H53A | Saccharomyces cerevisiae | nicotinamide | 0.014 | 0.0065 | 2.2 | pH 7.5, 25 °C | Open record: nicotinamidase (Pnc1) variant H53A, nicotinamide |
| nicotinamidase (Pnc1)variant H94A | Saccharomyces cerevisiae | nicotinamide | 0.02 | — | — | pH 7.5, 25 °C | Open record: nicotinamidase (Pnc1) variant H94A, nicotinamide |
| nicotinamidase (Pnc1)variant K122A | Saccharomyces cerevisiae | nicotinamide | 0.044 | — | — | pH 7.5, 25 °C | Open record: nicotinamidase (Pnc1) variant K122A, nicotinamide |
| nicotinamidase (Pnc1)variant K122R | Saccharomyces cerevisiae | nicotinamide | 9 × 10⁻⁴ | — | — | pH 7.5, 25 °C | Open record: nicotinamidase (Pnc1) variant K122R, nicotinamide |