Values CatLog read from this paper

N-acetyl lysyl-tRNA synthetases evolved by a CcdB-based selection possess N-acetyl lysine specificity in vitro and in vivo

PMID 22289181doi:10.1016/j.febslet.2012.01.029Open in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 3 records (6 values) from this paper: N-acetyl lysyl-tRNA synthetase MbAcKRS3 (pyrrolysyl-tRNA synthetase, PylRS, variant), N-acetyl lysyl-tRNA synthetase MmAcKRS1 (pyrrolysyl-tRNA synthetase, PylRS, variant), N-acetyl lysyl-tRNA synthetase MmAcKRS2 (pyrrolysyl-tRNA synthetase, PylRS, variant) from Methanosarcina mazei.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 3 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.

EC 6.1.1.26

Records read from this paper
EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)ConditionsOpen record
N-acetyl lysyl-tRNA synthetase MbAcKRS3 (pyrrolysyl-tRNA synthetase, PylRS, variant)variant L266M/L270I/Y271F/L274A/C313F—N-Acetyl lysine0.034122.30.00153 calc.Open record: N-acetyl lysyl-tRNA synthetase MbAcKRS3 (pyrrolysyl-tRNA synthetase, PylRS, variant) variant L266M/L270I/Y271F/L274A/C313F, N-Acetyl lysine
N-acetyl lysyl-tRNA synthetase MmAcKRS1 (pyrrolysyl-tRNA synthetase, PylRS, variant)variant L301M/Y306L/L309A/C348FMethanosarcina mazeiN-Acetyl lysine0.032335.39.15 × 10⁻⁴ calc.Open record: N-acetyl lysyl-tRNA synthetase MmAcKRS1 (pyrrolysyl-tRNA synthetase, PylRS, variant) variant L301M/Y306L/L309A/C348F, N-Acetyl lysine
N-acetyl lysyl-tRNA synthetase MmAcKRS2 (pyrrolysyl-tRNA synthetase, PylRS, variant)variant L301M/Y306L/C348S/A315VMethanosarcina mazeiN-Acetyl lysine0.007317.89.4 × 10⁻⁴ calc.Open record: N-acetyl lysyl-tRNA synthetase MmAcKRS2 (pyrrolysyl-tRNA synthetase, PylRS, variant) variant L301M/Y306L/C348S/A315V, N-Acetyl lysine