Values CatLog read from this paper
N-acetyl lysyl-tRNA synthetases evolved by a CcdB-based selection possess N-acetyl lysine specificity in vitro and in vivo
PMID 22289181doi:10.1016/j.febslet.2012.01.029Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 3 records (6 values) from this paper: N-acetyl lysyl-tRNA synthetase MbAcKRS3 (pyrrolysyl-tRNA synthetase, PylRS, variant), N-acetyl lysyl-tRNA synthetase MmAcKRS1 (pyrrolysyl-tRNA synthetase, PylRS, variant), N-acetyl lysyl-tRNA synthetase MmAcKRS2 (pyrrolysyl-tRNA synthetase, PylRS, variant) from Methanosarcina mazei.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 3 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| N-acetyl lysyl-tRNA synthetase MbAcKRS3 (pyrrolysyl-tRNA synthetase, PylRS, variant)variant L266M/L270I/Y271F/L274A/C313F | — | N-Acetyl lysine | 0.0341 | 22.3 | 0.00153 calc. | Open record: N-acetyl lysyl-tRNA synthetase MbAcKRS3 (pyrrolysyl-tRNA synthetase, PylRS, variant) variant L266M/L270I/Y271F/L274A/C313F, N-Acetyl lysine | |
| N-acetyl lysyl-tRNA synthetase MmAcKRS1 (pyrrolysyl-tRNA synthetase, PylRS, variant)variant L301M/Y306L/L309A/C348F | Methanosarcina mazei | N-Acetyl lysine | 0.0323 | 35.3 | 9.15 × 10⁻⁴ calc. | Open record: N-acetyl lysyl-tRNA synthetase MmAcKRS1 (pyrrolysyl-tRNA synthetase, PylRS, variant) variant L301M/Y306L/L309A/C348F, N-Acetyl lysine | |
| N-acetyl lysyl-tRNA synthetase MmAcKRS2 (pyrrolysyl-tRNA synthetase, PylRS, variant)variant L301M/Y306L/C348S/A315V | Methanosarcina mazei | N-Acetyl lysine | 0.00731 | 7.8 | 9.4 × 10⁻⁴ calc. | Open record: N-acetyl lysyl-tRNA synthetase MmAcKRS2 (pyrrolysyl-tRNA synthetase, PylRS, variant) variant L301M/Y306L/C348S/A315V, N-Acetyl lysine |