Values CatLog read from this paper
Structural basis for dual nucleotide selectivity of aminoglycoside 2''-phosphotransferase IVa provides insight on determinants of nucleotide specificity of aminoglycoside kinases
PMID 22371504doi:10.1074/jbc.m112.349670Open in Browse
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CatLog read 6 records (18 values) from this paper: aminoglycoside 2''-phosphotransferase IVa (APH(2'')-IVa).
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| aminoglycoside 2''-phosphotransferase IVa (APH(2'')-IVa) | — | adenosine 5'-triphosphate | 4.37 | 0.069 | 63 | pH 7.5, 37 °C | Open record: aminoglycoside 2''-phosphotransferase IVa (APH(2'')-IVa), adenosine 5'-triphosphate |
| aminoglycoside 2''-phosphotransferase IVa (APH(2'')-IVa) | — | guanosine 5'-triphosphate | 5.03 | 0.168 | 30 | pH 7.5, 37 °C | Open record: aminoglycoside 2''-phosphotransferase IVa (APH(2'')-IVa), guanosine 5'-triphosphate |
| aminoglycoside 2''-phosphotransferase IVa (APH(2'')-IVa)variant F95M | — | adenosine 5'-triphosphate | 3.06 | 0.055 | 55 | pH 7.5, 37 °C | Open record: aminoglycoside 2''-phosphotransferase IVa (APH(2'')-IVa) variant F95M, adenosine 5'-triphosphate |
| aminoglycoside 2''-phosphotransferase IVa (APH(2'')-IVa)variant F95M | — | guanosine 5'-triphosphate | 4.35 | 0.156 | 28 | pH 7.5, 37 °C | Open record: aminoglycoside 2''-phosphotransferase IVa (APH(2'')-IVa) variant F95M, guanosine 5'-triphosphate |
| aminoglycoside 2''-phosphotransferase IVa (APH(2'')-IVa)variant F95Y | — | adenosine 5'-triphosphate | 1.06 | 0.072 | 15 | pH 7.5, 37 °C | Open record: aminoglycoside 2''-phosphotransferase IVa (APH(2'')-IVa) variant F95Y, adenosine 5'-triphosphate |
| aminoglycoside 2''-phosphotransferase IVa (APH(2'')-IVa)variant F95Y | — | guanosine 5'-triphosphate | 1.15 | 0.049 | 23 | pH 7.5, 37 °C | Open record: aminoglycoside 2''-phosphotransferase IVa (APH(2'')-IVa) variant F95Y, guanosine 5'-triphosphate |