Values CatLog read from this paper
Conversion of human steroid 5β-reductase (AKR1D1) into 3β-hydroxysteroid dehydrogenase by single point mutation E120H: example of perfect enzyme engineering
PMID 22437839doi:10.1074/jbc.m111.338780Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 10 records (30 values) from this paper: AKR1D1 (steroid 5beta-reductase) from Homo sapiens.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| AKR1D1 (steroid 5beta-reductase)variant E120H | Homo sapiens | 17β-Estradiol | 0.00587 | 0.0028 | 2.1 | pH 7, 37 °C | Open record: AKR1D1 (steroid 5beta-reductase) variant E120H, 17β-Estradiol |
| AKR1D1 (steroid 5beta-reductase)variant E120H | Homo sapiens | 3α-Hydroxy-5β-androstan-17-one | 0.125 | 0.0364 | 3.4 | pH 7, 37 °C | Open record: AKR1D1 (steroid 5beta-reductase) variant E120H, 3α-Hydroxy-5β-androstan-17-one |
| AKR1D1 (steroid 5beta-reductase)variant E120H | Homo sapiens | 3β-hydroxy-5α-androstan-17-one | 0.253 | 0.0019 | 130 | pH 7, 37 °C | Open record: AKR1D1 (steroid 5beta-reductase) variant E120H, 3β-hydroxy-5α-androstan-17-one |
| AKR1D1 (steroid 5beta-reductase)variant E120H | Homo sapiens | 3β-Hydroxy-5β-androstan-17-one | 0.043 | 0.0153 | 2.8 | pH 7, 37 °C | Open record: AKR1D1 (steroid 5beta-reductase) variant E120H, 3β-Hydroxy-5β-androstan-17-one |
| AKR1D1 (steroid 5beta-reductase)variant E120H | Homo sapiens | 5α-dihydrotestosterone | 0.0338 | 1.6 × 10⁻⁴ | 210 | pH 6, 37 °C | Open record: AKR1D1 (steroid 5beta-reductase) variant E120H, 5α-dihydrotestosterone |
| AKR1D1 (steroid 5beta-reductase)variant E120H | Homo sapiens | 5α-dihydrotestosterone | 0.0058 | 8 × 10⁻⁴ | 7.3 | pH 7, 37 °C | Open record: AKR1D1 (steroid 5beta-reductase) variant E120H, 5α-dihydrotestosterone |
| AKR1D1 (steroid 5beta-reductase)variant E120H | Homo sapiens | 5β-dihydrotestosterone | 0.0268 | 0.0015 | 18 | pH 6, 37 °C | Open record: AKR1D1 (steroid 5beta-reductase) variant E120H, 5β-dihydrotestosterone |
| AKR1D1 (steroid 5beta-reductase)variant E120H | Homo sapiens | 5β-dihydrotestosterone | 8.5 × 10⁻⁴ | 0.0057 | 0.15 | pH 7, 37 °C | Open record: AKR1D1 (steroid 5beta-reductase) variant E120H, 5β-dihydrotestosterone |
| AKR1D1 (steroid 5beta-reductase)variant E120H | Homo sapiens | Androsterone | 0.047 | 0.0275 | 1.7 | pH 7, 37 °C | Open record: AKR1D1 (steroid 5beta-reductase) variant E120H, Androsterone |
| AKR1D1 (steroid 5beta-reductase)variant E120H | Homo sapiens | Testosterone | 7.7 × 10⁻⁴ | 0.0018 | 0.42 | pH 7, 37 °C | Open record: AKR1D1 (steroid 5beta-reductase) variant E120H, Testosterone |