EC 1.3.1.3: DELTA4-3-oxosteroid 5beta-reductase
OxidoreductasesOpen in Browse
CatLog read 43 records for EC 1.3.1.3 from 7 papers, for 4 organisms. Names in these papers: AKR1D1 (steroid 5beta-reductase), steroid 5β-reductase (AKR1D1), Δ4-3-ketosteroid 5β-reductase, AKR1D1 and 3 more.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units.
CatLog also holds 30 records for EC 1.3.1.3 that are not yet re-read: from its earlier version, built from the enzyme databases and partly checked against the papers. All records for EC 1.3.1.3 in Browse.
Papers
Records
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Paper | Open record |
|---|---|---|---|---|---|---|---|---|
| AKR1D1 | Homo sapiens | 17alpha,21-dihydroxypregn-4-ene-3,11,20-trione | 0.165 | 0.0151 | 11 | PMID 20522910 | Open record: AKR1D1, 17alpha,21-dihydroxypregn-4-ene-3,11,20-trione | |
| AKR1D1 | Homo sapiens | 17beta-hydroxyandrost-4-en-3-onefluorimetric assay; saturating NADPH; steroid 0.60-40 µM | 0.118 | 0.0027 | 43.8 | PMID 20522910 | Open record: AKR1D1, 17beta-hydroxyandrost-4-en-3-one | |
| AKR1D1 | Homo sapiens | 17beta-hydroxyandrost-4-en-3-oneintact transfected HEK 293 cells in DMEM; untagged | — | 0.004 | — | PMID 20522910 | Open record: AKR1D1, 17beta-hydroxyandrost-4-en-3-one | |
| AKR1D1 | Homo sapiens | 7alpha-hydroxycholest-4-en-3-one | — | 8 × 10⁻⁴ | — | PMID 20522910 | Open record: AKR1D1, 7alpha-hydroxycholest-4-en-3-one | |
| AKR1D1variant P133R | Homo sapiens | 17alpha,21-dihydroxypregn-4-ene-3,11,20-trione | 0.01 | 0.0013 | 7.7 | PMID 20522910 | Open record: AKR1D1 variant P133R, 17alpha,21-dihydroxypregn-4-ene-3,11,20-trione | |
| AKR1D1variant P133R | Homo sapiens | 17beta-hydroxyandrost-4-en-3-one | 0.045 | 0.0127 | 3.5 | PMID 20522910 | Open record: AKR1D1 variant P133R, 17beta-hydroxyandrost-4-en-3-one | |
| AKR1D1 (steroid 5beta-reductase)variant E120H | Homo sapiens | 17β-Estradiol | 0.00587 | 0.0028 | 2.1 | pH 7, 37 °C | PMID 22437839 | Open record: AKR1D1 (steroid 5beta-reductase) variant E120H, 17β-Estradiol |
| AKR1D1 (steroid 5beta-reductase)variant E120H | Homo sapiens | 3α-Hydroxy-5β-androstan-17-one | 0.125 | 0.0364 | 3.4 | pH 7, 37 °C | PMID 22437839 | Open record: AKR1D1 (steroid 5beta-reductase) variant E120H, 3α-Hydroxy-5β-androstan-17-one |
| AKR1D1 (steroid 5beta-reductase)variant E120H | Homo sapiens | 3β-hydroxy-5α-androstan-17-one | 0.253 | 0.0019 | 130 | pH 7, 37 °C | PMID 22437839 | Open record: AKR1D1 (steroid 5beta-reductase) variant E120H, 3β-hydroxy-5α-androstan-17-one |
| AKR1D1 (steroid 5beta-reductase)variant E120H | Homo sapiens | 3β-Hydroxy-5β-androstan-17-one | 0.043 | 0.0153 | 2.8 | pH 7, 37 °C | PMID 22437839 | Open record: AKR1D1 (steroid 5beta-reductase) variant E120H, 3β-Hydroxy-5β-androstan-17-one |
| AKR1D1 (steroid 5beta-reductase)variant E120H | Homo sapiens | 5α-dihydrotestosterone | 0.0338 | 1.6 × 10⁻⁴ | 210 | pH 6, 37 °C | PMID 22437839 | Open record: AKR1D1 (steroid 5beta-reductase) variant E120H, 5α-dihydrotestosterone |
| AKR1D1 (steroid 5beta-reductase)variant E120H | Homo sapiens | 5α-dihydrotestosterone | 0.0058 | 8 × 10⁻⁴ | 7.3 | pH 7, 37 °C | PMID 22437839 | Open record: AKR1D1 (steroid 5beta-reductase) variant E120H, 5α-dihydrotestosterone |
| AKR1D1 (steroid 5beta-reductase)variant E120H | Homo sapiens | 5β-dihydrotestosterone | 0.0268 | 0.0015 | 18 | pH 6, 37 °C | PMID 22437839 | Open record: AKR1D1 (steroid 5beta-reductase) variant E120H, 5β-dihydrotestosterone |
| AKR1D1 (steroid 5beta-reductase)variant E120H | Homo sapiens | 5β-dihydrotestosterone | 8.5 × 10⁻⁴ | 0.0057 | 0.15 | pH 7, 37 °C | PMID 22437839 | Open record: AKR1D1 (steroid 5beta-reductase) variant E120H, 5β-dihydrotestosterone |
| AKR1D1 (steroid 5beta-reductase)variant E120H | Homo sapiens | Androsterone | 0.047 | 0.0275 | 1.7 | pH 7, 37 °C | PMID 22437839 | Open record: AKR1D1 (steroid 5beta-reductase) variant E120H, Androsterone |
| AKR1D1 (steroid 5beta-reductase)variant E120H | Homo sapiens | Testosterone | 7.7 × 10⁻⁴ | 0.0018 | 0.42 | pH 7, 37 °C | PMID 22437839 | Open record: AKR1D1 (steroid 5beta-reductase) variant E120H, Testosterone |
| human delta4-3-ketosteroid 5beta-reductase (h5beta-red, AKR1D1) | Homo sapiens | androstenedione | 0.013 | 3.66 × 10⁻⁴ | 35.5 | pH 7.3, 37 °C | PMID 19075558 | Open record: human delta4-3-ketosteroid 5beta-reductase (h5beta-red, AKR1D1), androstenedione |
| human delta4-3-ketosteroid 5beta-reductase (h5beta-red, AKR1D1)variant V309F | Homo sapiens | androstenedione | 0.0425 | 0.01629 | 2.6 | pH 7.3, 37 °C | PMID 19075558 | Open record: human delta4-3-ketosteroid 5beta-reductase (h5beta-red, AKR1D1) variant V309F, androstenedione |
| human delta4-3-ketosteroid 5beta-reductase (h5beta-red, AKR1D1)variant Y132F | Homo sapiens | androstenedione | 0.0405 | 9.74 × 10⁻⁴ | 41.5 | pH 7.3, 37 °C | PMID 19075558 | Open record: human delta4-3-ketosteroid 5beta-reductase (h5beta-red, AKR1D1) variant Y132F, androstenedione |
| PRISE1 (Arabidopsis thaliana 1) | Arabidopsis thaliana | 8-oxogeranial | 0.155 | 0.0418 | 3.71 | pH 7, 25 °C | PMID 32501002 | Open record: PRISE1 (Arabidopsis thaliana 1), 8-oxogeranial |
| PRISE1 (Arabidopsis thaliana 1) | Arabidopsis thaliana | progesterone | 1.865 | 0.006 | 312.1 | pH 7, 25 °C | PMID 32501002 | Open record: PRISE1 (Arabidopsis thaliana 1), progesterone |
| progesterone 5beta-reductase (5beta-POR) | Digitalis lanata | 4-androstene-3,17-dione | — | 0.228 | — | 30 °C | PMID 16386278 | Open record: progesterone 5beta-reductase (5beta-POR), 4-androstene-3,17-dione |
| progesterone 5beta-reductase (5beta-POR) | Digitalis lanata | Cortexone | — | 1.597 | — | 30 °C | PMID 16386278 | Open record: progesterone 5beta-reductase (5beta-POR), Cortexone |
| progesterone 5beta-reductase (5beta-POR) | Digitalis lanata | Cortisol | — | 0.291 | — | 30 °C | PMID 16386278 | Open record: progesterone 5beta-reductase (5beta-POR), Cortisol |
| progesterone 5beta-reductase (5beta-POR) | Digitalis lanata | NADPH | — | 0.008 | — | 30 °C | PMID 16386278 | Open record: progesterone 5beta-reductase (5beta-POR), NADPH |
| progesterone 5beta-reductase (5beta-POR) | Digitalis lanata | Progesterone | — | 0.12 | — | 30 °C | PMID 16386278 | Open record: progesterone 5beta-reductase (5beta-POR), Progesterone |
| steroid 5β-reductase (AKR1D1) | Homo sapiens | 17α-hydroxyandrost-4-en-3-one | 0.1 | 0.0029 | 34.8 | pH 6, 37 °C | PMID 21255593 | Open record: steroid 5β-reductase (AKR1D1), 17α-hydroxyandrost-4-en-3-one |
| steroid 5β-reductase (AKR1D1) | Homo sapiens | 17β-hydroxyestr-4-en-3-one | 0.045 | 0.003 | 15.2 | pH 6, 37 °C | PMID 21255593 | Open record: steroid 5β-reductase (AKR1D1), 17β-hydroxyestr-4-en-3-one |
| steroid 5β-reductase (AKR1D1) | Homo sapiens | 7α-hydroxycholest-4-en-3-one | 0.033 | 8 × 10⁻⁴ | 42.2 | pH 6, 37 °C | PMID 21255593 | Open record: steroid 5β-reductase (AKR1D1), 7α-hydroxycholest-4-en-3-one |
| steroid 5β-reductase (AKR1D1) | Homo sapiens | Aldosterone | 0.15 | 0.0025 | 61.3 | pH 6, 37 °C | PMID 21255593 | Open record: steroid 5β-reductase (AKR1D1), Aldosterone |
| steroid 5β-reductase (AKR1D1) | Homo sapiens | androst-4-ene-3,17-dione | 0.1 | 9 × 10⁻⁴ | 112 | pH 6, 37 °C | PMID 21255593 | Open record: steroid 5β-reductase (AKR1D1), androst-4-ene-3,17-dione |
| steroid 5β-reductase (AKR1D1) | Homo sapiens | cholest-4-en-3-one | 0.01 | 3 × 10⁻⁴ | 33 | pH 6, 37 °C | PMID 21255593 | Open record: steroid 5β-reductase (AKR1D1), cholest-4-en-3-one |
| steroid 5β-reductase (AKR1D1) | Homo sapiens | Corticosterone | 0.032 | 0.0022 | 14.5 | pH 6, 37 °C | PMID 21255593 | Open record: steroid 5β-reductase (AKR1D1), Corticosterone |
| steroid 5β-reductase (AKR1D1) | Homo sapiens | Cortisol | 0.045 | 0.0131 | 3.5 | pH 6, 37 °C | PMID 21255593 | Open record: steroid 5β-reductase (AKR1D1), Cortisol |
| steroid 5β-reductase (AKR1D1) | Homo sapiens | Cortisone | 0.195 | 0.0151 | 13 | pH 6, 37 °C | PMID 21255593 | Open record: steroid 5β-reductase (AKR1D1), Cortisone |
| Δ4-3-ketosteroid 5β-reductase | Rattus norvegicus | 4-Androstene-3,17-dione | 0.0337 | 0.00485 | 6.94 calc. | pH 7.4, 25 °C | PMID 6736016 | Open record: Δ4-3-ketosteroid 5β-reductase, 4-Androstene-3,17-dione |
| Δ4-3-ketosteroid 5β-reductase | Rattus norvegicus | 7α,12α-Dihydroxy-4-cholesten-3-one | 0.0128 | 0.0122 | 1.10 calc. | pH 7.4, 25 °C | PMID 6736016 | Open record: Δ4-3-ketosteroid 5β-reductase, 7α,12α-Dihydroxy-4-cholesten-3-one |
| Δ4-3-ketosteroid 5β-reductase | Rattus norvegicus | 7α-Hydroxy-4-cholesten-3-one | 0.01 | 0.0091 | 1.1 calc. | pH 7.4 | PMID 6736016 | Open record: Δ4-3-ketosteroid 5β-reductase, 7α-Hydroxy-4-cholesten-3-one |
| Δ4-3-ketosteroid 5β-reductase | Rattus norvegicus | Cortisol | 0.0082 | 0.011 | 0.74 calc. | pH 7.4, 25 °C | PMID 6736016 | Open record: Δ4-3-ketosteroid 5β-reductase, Cortisol |
| Δ4-3-ketosteroid 5β-reductase | Rattus norvegicus | Cortisone | 0.0375 | 0.0217 | 1.73 calc. | pH 7.4, 25 °C | PMID 6736016 | Open record: Δ4-3-ketosteroid 5β-reductase, Cortisone |
| Δ4-3-ketosteroid 5β-reductase | Rattus norvegicus | NADPH | — | 0.003 | — | pH 7.4 | PMID 6736016 | Open record: Δ4-3-ketosteroid 5β-reductase, NADPH |
| Δ4-3-ketosteroid 5β-reductase | Rattus norvegicus | Progesterone | 0.0302 | 0.00219 | 13.8 calc. | pH 7.4, 25 °C | PMID 6736016 | Open record: Δ4-3-ketosteroid 5β-reductase, Progesterone |
| Δ4-3-ketosteroid 5β-reductase | Rattus norvegicus | Testosterone | 0.0208 | 0.0024 | 8.7 calc. | pH 7.4, 25 °C | PMID 6736016 | Open record: Δ4-3-ketosteroid 5β-reductase, Testosterone |