CatLogenzyme kinetics catalog

Values CatLog read from this paper

Purification and characterization of delta 4-3-ketosteroid 5 beta-reductase

PMID 6736016doi:10.1016/s0021-9258(17)42821-3Open in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 8 records (15 values) from this paper: Δ4-3-ketosteroid 5β-reductase from Rattus norvegicus.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 7 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.

EC 1.3.1.3

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)Conditions
Δ4-3-ketosteroid 5β-reductaseRattus norvegicus4-Androstene-3,17-dione0.03370.004856.94 calc.pH 7.4, 25 °COpen
Δ4-3-ketosteroid 5β-reductaseRattus norvegicus7α,12α-Dihydroxy-4-cholesten-3-one0.01280.01221.10 calc.pH 7.4, 25 °COpen
Δ4-3-ketosteroid 5β-reductaseRattus norvegicus7α-Hydroxy-4-cholesten-3-one0.010.00911.1 calc.pH 7.4Open
Δ4-3-ketosteroid 5β-reductaseRattus norvegicusCortisol0.00820.0110.74 calc.pH 7.4, 25 °COpen
Δ4-3-ketosteroid 5β-reductaseRattus norvegicusCortisone0.03750.02171.73 calc.pH 7.4, 25 °COpen
Δ4-3-ketosteroid 5β-reductaseRattus norvegicusNADPH—0.003—pH 7.4Open
Δ4-3-ketosteroid 5β-reductaseRattus norvegicusProgesterone0.03020.0021913.8 calc.pH 7.4, 25 °COpen
Δ4-3-ketosteroid 5β-reductaseRattus norvegicusTestosterone0.02080.00248.7 calc.pH 7.4, 25 °COpen