Values CatLog read from this paper
The enzymology of alanine aminotransferase (AlaAT) isoforms from Hordeum vulgare and other organisms, and the HvAlaAT crystal structure
PMID 22750542doi:10.1016/j.abb.2012.06.006Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 36 records (54 values) from this paper: alanine aminotransferase (HvAlaAT, N-terminal His-tagged), alanine aminotransferase (Os AlaAT), alanine aminotransferase (Sc AlaAT) and 6 more from Hordeum vulgare, Zea mays, Oryza sativa and 4 more.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 18 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| alanine aminotransferase (HvAlaAT, N-terminal His-tagged) | Hordeum vulgare | 2-oxoglutarate | — | 0.18 | — | pH 8, 25 °C | Open record: alanine aminotransferase (HvAlaAT, N-terminal His-tagged), 2-oxoglutarate |
| alanine aminotransferase (HvAlaAT, N-terminal His-tagged) | Hordeum vulgare | L-alanine | 8.27 | 0.83 | 10 calc. | pH 8, 25 °C | Open record: alanine aminotransferase (HvAlaAT, N-terminal His-tagged), L-alanine |
| alanine aminotransferase (HvAlaAT, N-terminal His-tagged) | Hordeum vulgare | L-glutamate | 9.09 | 0.54 | 17 calc. | pH 8, 25 °C | Open record: alanine aminotransferase (HvAlaAT, N-terminal His-tagged), L-glutamate |
| alanine aminotransferase (HvAlaAT, N-terminal His-tagged) | Hordeum vulgare | pyruvate | — | 0.21 | — | pH 8, 25 °C | Open record: alanine aminotransferase (HvAlaAT, N-terminal His-tagged), pyruvate |
| alanine aminotransferase (Os AlaAT) | Oryza sativa | 2-oxoglutarate | — | 0.25 | — | pH 8, 25 °C | Open record: alanine aminotransferase (Os AlaAT), 2-oxoglutarate |
| alanine aminotransferase (Os AlaAT) | Oryza sativa | L-alanine | 4.98 | 0.4 | 12 calc. | pH 8, 25 °C | Open record: alanine aminotransferase (Os AlaAT), L-alanine |
| alanine aminotransferase (Os AlaAT) | Oryza sativa | L-glutamate | 6.86 | 0.68 | 10 calc. | pH 8, 25 °C | Open record: alanine aminotransferase (Os AlaAT), L-glutamate |
| alanine aminotransferase (Os AlaAT) | Oryza sativa | pyruvate | — | 0.11 | — | pH 8, 25 °C | Open record: alanine aminotransferase (Os AlaAT), pyruvate |
| alanine aminotransferase (Sc AlaAT) | Saccharomyces cerevisiae | 2-oxoglutarate | — | 0.2 | — | pH 8, 25 °C | Open record: alanine aminotransferase (Sc AlaAT), 2-oxoglutarate |
| alanine aminotransferase (Sc AlaAT) | Saccharomyces cerevisiae | L-alanine | 6.09 | 3.6 | 1.7 calc. | pH 8, 25 °C | Open record: alanine aminotransferase (Sc AlaAT), L-alanine |
| alanine aminotransferase (Sc AlaAT) | Saccharomyces cerevisiae | L-glutamate | 8.64 | 0.24 | 36 calc. | pH 8, 25 °C | Open record: alanine aminotransferase (Sc AlaAT), L-glutamate |
| alanine aminotransferase (Sc AlaAT) | Saccharomyces cerevisiae | pyruvate | — | 0.4 | — | pH 8, 25 °C | Open record: alanine aminotransferase (Sc AlaAT), pyruvate |
| alanine aminotransferase (tag-free HvAlaAT) | Hordeum vulgare | 2-oxoglutarate | — | 0.28 | — | pH 8, 25 °C | Open record: alanine aminotransferase (tag-free HvAlaAT), 2-oxoglutarate |
| alanine aminotransferase (tag-free HvAlaAT) | Hordeum vulgare | L-alanine | 12.11 | 3.79 | 3.20 calc. | pH 8, 25 °C | Open record: alanine aminotransferase (tag-free HvAlaAT), L-alanine |
| alanine aminotransferase (tag-free HvAlaAT) | Hordeum vulgare | L-glutamate | 25.62 | 0.79 | 32 calc. | pH 8, 25 °C | Open record: alanine aminotransferase (tag-free HvAlaAT), L-glutamate |
| alanine aminotransferase (tag-free HvAlaAT) | Hordeum vulgare | pyruvate | — | 0.17 | — | pH 8, 25 °C | Open record: alanine aminotransferase (tag-free HvAlaAT), pyruvate |
| alanine aminotransferase 1 (Zm AlaAT 1) | Zea mays | 2-oxoglutarate | — | 0.37 | — | pH 8, 25 °C | Open record: alanine aminotransferase 1 (Zm AlaAT 1), 2-oxoglutarate |
| alanine aminotransferase 1 (Zm AlaAT 1) | Zea mays | L-alanine | 5.49 | 0.62 | 8.9 calc. | pH 8, 25 °C | Open record: alanine aminotransferase 1 (Zm AlaAT 1), L-alanine |
| alanine aminotransferase 1 (Zm AlaAT 1) | Zea mays | L-glutamate | 6.56 | 0.56 | 12 calc. | pH 8, 25 °C | Open record: alanine aminotransferase 1 (Zm AlaAT 1), L-glutamate |
| alanine aminotransferase 1 (Zm AlaAT 1) | Zea mays | pyruvate | — | 0.42 | — | pH 8, 25 °C | Open record: alanine aminotransferase 1 (Zm AlaAT 1), pyruvate |
| alanine aminotransferase 2 (Zm AlaAT 2) | Zea mays | 2-oxoglutarate | — | 0.2 | — | pH 8, 25 °C | Open record: alanine aminotransferase 2 (Zm AlaAT 2), 2-oxoglutarate |
| alanine aminotransferase 2 (Zm AlaAT 2) | Zea mays | L-alanine | 0.33 | 3.06 | 0.11 calc. | pH 8, 25 °C | Open record: alanine aminotransferase 2 (Zm AlaAT 2), L-alanine |
| alanine aminotransferase 2 (Zm AlaAT 2) | Zea mays | L-glutamate | 0.67 | 0.55 | 1.2 calc. | pH 8, 25 °C | Open record: alanine aminotransferase 2 (Zm AlaAT 2), L-glutamate |
| alanine aminotransferase 2 (Zm AlaAT 2) | Zea mays | pyruvate | — | 0.54 | — | pH 8, 25 °C | Open record: alanine aminotransferase 2 (Zm AlaAT 2), pyruvate |
| beta-alanine aminotransferase (Pp β-AlaAT) | Pseudomonas putida | 2-oxoglutarate | — | 0.05 | — | pH 8, 25 °C | Open record: beta-alanine aminotransferase (Pp β-AlaAT), 2-oxoglutarate |
| beta-alanine aminotransferase (Pp β-AlaAT) | Pseudomonas putida | L-alanine | 0.08 | 0.21 | 0.38 calc. | pH 8, 25 °C | Open record: beta-alanine aminotransferase (Pp β-AlaAT), L-alanine |
| beta-alanine aminotransferase (Pp β-AlaAT) | Pseudomonas putida | L-glutamate | 0.17 | 0.57 | 0.30 calc. | pH 8, 25 °C | Open record: beta-alanine aminotransferase (Pp β-AlaAT), L-glutamate |
| beta-alanine aminotransferase (Pp β-AlaAT) | Pseudomonas putida | pyruvate | — | 0.2 | — | pH 8, 25 °C | Open record: beta-alanine aminotransferase (Pp β-AlaAT), pyruvate |
| D-alanine aminotransferase (Bh d-AlaAT) | Bacillus halodurans | 2-oxoglutarate | — | 0.32 | — | pH 8, 25 °C | Open record: D-alanine aminotransferase (Bh d-AlaAT), 2-oxoglutarate |
| D-alanine aminotransferase (Bh d-AlaAT) | Bacillus halodurans | L-alanine | 1.25 | 5.15 | 0.243 calc. | pH 8, 25 °C | Open record: D-alanine aminotransferase (Bh d-AlaAT), L-alanine |
| D-alanine aminotransferase (Bh d-AlaAT) | Bacillus halodurans | L-glutamate | 2.8 | 0.44 | 6.4 calc. | pH 8, 25 °C | Open record: D-alanine aminotransferase (Bh d-AlaAT), L-glutamate |
| D-alanine aminotransferase (Bh d-AlaAT) | Bacillus halodurans | pyruvate | — | 0.08 | — | pH 8, 25 °C | Open record: D-alanine aminotransferase (Bh d-AlaAT), pyruvate |
| D-alanine aminotransferase (Rp d-AlaAT) | Rhodopseudomonas palustris | 2-oxoglutarate | — | 0.2 | — | pH 8, 25 °C | Open record: D-alanine aminotransferase (Rp d-AlaAT), 2-oxoglutarate |
| D-alanine aminotransferase (Rp d-AlaAT) | Rhodopseudomonas palustris | L-alanine | 0.38 | 1.52 | 0.25 calc. | pH 8, 25 °C | Open record: D-alanine aminotransferase (Rp d-AlaAT), L-alanine |
| D-alanine aminotransferase (Rp d-AlaAT) | Rhodopseudomonas palustris | L-glutamate | 3.06 | 0.2 | 15 calc. | pH 8, 25 °C | Open record: D-alanine aminotransferase (Rp d-AlaAT), L-glutamate |
| D-alanine aminotransferase (Rp d-AlaAT) | Rhodopseudomonas palustris | pyruvate | — | 0.08 | — | pH 8, 25 °C | Open record: D-alanine aminotransferase (Rp d-AlaAT), pyruvate |