Values CatLog read from this paper
Structural insights into decreased enzymatic activity induced by an insert sequence in mannonate dehydratase from Gram negative bacterium
PMID 22796868doi:10.1016/j.jsb.2012.06.013Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 8 records (16 values) from this paper: mannonate dehydratase (EcManD) from Escherichia coli.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 8 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| mannonate dehydratase (EcManD) | Escherichia coli | D-mannonate | 0.81 | 4.79 | 0.17 calc. | pH 7.5, 37 °C | Open record: mannonate dehydratase (EcManD), D-mannonate |
| mannonate dehydratase (EcManD)variant E185D | Escherichia coli | D-mannonate | 0.23 | 2.31 | 0.10 calc. | pH 7.5, 37 °C | Open record: mannonate dehydratase (EcManD) variant E185D, D-mannonate |
| mannonate dehydratase (EcManD)variant E185G | Escherichia coli | D-mannonate | 0.94 | 4.55 | 0.21 calc. | pH 7.5, 37 °C | Open record: mannonate dehydratase (EcManD) variant E185G, D-mannonate |
| mannonate dehydratase (EcManD)variant E186D | Escherichia coli | D-mannonate | 0.29 | 3.6 | 0.081 calc. | pH 7.5, 37 °C | Open record: mannonate dehydratase (EcManD) variant E186D, D-mannonate |
| mannonate dehydratase (EcManD)variant E186G | Escherichia coli | D-mannonate | 1.1 | 5.3 | 0.21 calc. | pH 7.5, 37 °C | Open record: mannonate dehydratase (EcManD) variant E186G, D-mannonate |
| mannonate dehydratase (EcManD)variant H32G | Escherichia coli | D-mannonate | 1.29 | 5.21 | 0.248 calc. | pH 7.5, 37 °C | Open record: mannonate dehydratase (EcManD) variant H32G, D-mannonate |
| mannonate dehydratase (EcManD)variant N103A/D109A | Escherichia coli | D-mannonate | 0.1 | 4.08 | 0.025 calc. | pH 7.5, 37 °C | Open record: mannonate dehydratase (EcManD) variant N103A/D109A, D-mannonate |
| mannonate dehydratase (EcManD)variant N36G | Escherichia coli | D-mannonate | 1.4 | 4.67 | 0.30 calc. | pH 7.5, 37 °C | Open record: mannonate dehydratase (EcManD) variant N36G, D-mannonate |