Values CatLog read from this paper
Entamoeba histolytica thioredoxin reductase: molecular and functional characterization of its atypical properties
PMID 22967759doi:10.1016/j.bbagen.2012.08.020Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 28 records (64 values) from this paper: thioredoxin reductase (EhTRXR) from Entamoeba histolytica.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| thioredoxin reductase (EhTRXR) | Entamoeba histolytica | 1-chloro-2,4-dinitrobenzene | 4.97 | 0.105 | 47 | pH 7, 30 °C | Open record: thioredoxin reductase (EhTRXR), 1-chloro-2,4-dinitrobenzene |
| thioredoxin reductase (EhTRXR) | Entamoeba histolytica | 2,5-di-tert-butyl-1,4-benzoquinone | 0.95 | 0.007 | 140 | pH 7, 30 °C | Open record: thioredoxin reductase (EhTRXR), 2,5-di-tert-butyl-1,4-benzoquinone |
| thioredoxin reductase (EhTRXR) | Entamoeba histolytica | 2-methyl-1,4-benzoquinone | 9.47 | 0.092 | 100 | pH 7, 30 °C | Open record: thioredoxin reductase (EhTRXR), 2-methyl-1,4-benzoquinone |
| thioredoxin reductase (EhTRXR) | Entamoeba histolytica | 5,5'-dithiobis(2-nitrobenzoic acid)0.3 mM NADPH; cosubstrate NADPH (0.3 mM) | 0.25 | 1.1 | 0.23 | pH 7, 30 °C | Open record: thioredoxin reductase (EhTRXR), 5,5'-dithiobis(2-nitrobenzoic acid) |
| thioredoxin reductase (EhTRXR) | Entamoeba histolytica | 5,5'-dithiobis(2-nitrobenzoic acid)0.4 mM NADH; cosubstrate NADH (0.4 mM) | 0.23 | 1.4 | 0.17 | pH 7, 30 °C | Open record: thioredoxin reductase (EhTRXR), 5,5'-dithiobis(2-nitrobenzoic acid) |
| thioredoxin reductase (EhTRXR) | Entamoeba histolytica | Entamoeba histolytica thioredoxin EhTRX41 (oxidized)0.3 mM NADPH; cosubstrate NADPH (0.3 mM) | 2.2 | 0.0036 | 600 | pH 7, 30 °C | Open record: thioredoxin reductase (EhTRXR), Entamoeba histolytica thioredoxin EhTRX41 (oxidized) |
| thioredoxin reductase (EhTRXR) | Entamoeba histolytica | Entamoeba histolytica thioredoxin EhTRX41 (oxidized)0.4 mM NADH; cosubstrate NADH (0.4 mM) | 2.03 | 0.0023 | 880 | pH 7, 30 °C | Open record: thioredoxin reductase (EhTRXR), Entamoeba histolytica thioredoxin EhTRX41 (oxidized) |
| thioredoxin reductase (EhTRXR) | Entamoeba histolytica | Entamoeba histolytica thioredoxin EhTRX8 (oxidized)0.3 mM NADPH; cosubstrate NADPH (0.3 mM) | 2.7 | 0.0028 | 950 | pH 7, 30 °C | Open record: thioredoxin reductase (EhTRXR), Entamoeba histolytica thioredoxin EhTRX8 (oxidized) |
| thioredoxin reductase (EhTRXR) | Entamoeba histolytica | Entamoeba histolytica thioredoxin EhTRX8 (oxidized)0.4 mM NADH; cosubstrate NADH (0.4 mM) | 2.58 | 0.0029 | 890 | pH 7, 30 °C | Open record: thioredoxin reductase (EhTRXR), Entamoeba histolytica thioredoxin EhTRX8 (oxidized) |
| thioredoxin reductase (EhTRXR) | Entamoeba histolytica | Escherichia coli thioredoxin (oxidized)thioredoxin reductase assay; 0.3 mM NADPH | 1.25 | 0.0047 | 260 | pH 7, 30 °C | Open record: thioredoxin reductase (EhTRXR), Escherichia coli thioredoxin (oxidized) |
| thioredoxin reductase (EhTRXR) | Entamoeba histolytica | Escherichia coli thioredoxin (oxidized)thioredoxin reductase assay; 0.4 mM NADH | 0.5 | 0.0062 | 75 | pH 7, 30 °C | Open record: thioredoxin reductase (EhTRXR), Escherichia coli thioredoxin (oxidized) |
| thioredoxin reductase (EhTRXR) | Entamoeba histolytica | Escherichia coli thioredoxin (oxidized)redox potential determination; NADPH oxidation at 340 nm | — | — | 295 | pH 7, 30 °C | Open record: thioredoxin reductase (EhTRXR), Escherichia coli thioredoxin (oxidized) |
| thioredoxin reductase (EhTRXR) | Entamoeba histolytica | Escherichia coli thioredoxin (reduced) | — | — | 118 | pH 7, 30 °C | Open record: thioredoxin reductase (EhTRXR), Escherichia coli thioredoxin (reduced) |
| thioredoxin reductase (EhTRXR) | Entamoeba histolytica | methyl viologen | 2.68 | 0.184 | 14 | pH 7, 30 °C | Open record: thioredoxin reductase (EhTRXR), methyl viologen |
| thioredoxin reductase (EhTRXR) | Entamoeba histolytica | methylene blue | 3.5 | 0.02 | 170 | pH 7, 30 °C | Open record: thioredoxin reductase (EhTRXR), methylene blue |
| thioredoxin reductase (EhTRXR) | Entamoeba histolytica | NADHcosubstrate DTNB (5 mM) | 0.2 | 0.023 | 84 | pH 7, 30 °C | Open record: thioredoxin reductase (EhTRXR), NADH |
| thioredoxin reductase (EhTRXR) | Entamoeba histolytica | NADHH2O2 production by ferrithiocyanate method; 300 μM NAD(P)H | 0.37 | — | — | pH 7, 30 °C | Open record: thioredoxin reductase (EhTRXR), NADH |
| thioredoxin reductase (EhTRXR) | Entamoeba histolytica | NADP+ | — | — | 17 | pH 7, 30 °C | Open record: thioredoxin reductase (EhTRXR), NADP+ |
| thioredoxin reductase (EhTRXR) | Entamoeba histolytica | NADPHDTNB (5 mM) | 0.25 | 0.0018 | 140 | pH 7, 30 °C | Open record: thioredoxin reductase (EhTRXR), NADPH |
| thioredoxin reductase (EhTRXR) | Entamoeba histolytica | NADPHredox potential determination; NADPH oxidation at 340 nm | — | — | 158 | pH 7, 30 °C | Open record: thioredoxin reductase (EhTRXR), NADPH |
| thioredoxin reductase (EhTRXR) | Entamoeba histolytica | NADPHH2O2 production by ferrithiocyanate method; 300 μM NAD(P)H | 0.37 | — | — | pH 7, 30 °C | Open record: thioredoxin reductase (EhTRXR), NADPH |
| thioredoxin reductase (EhTRXR) | Entamoeba histolytica | potassium ferricyanide (hexacyanoferrate(III)) | 6.2 | 0.293 | 21 | pH 7, 30 °C | Open record: thioredoxin reductase (EhTRXR), potassium ferricyanide (hexacyanoferrate(III)) |
| thioredoxin reductase (EhTRXR) | Entamoeba histolytica | S-nitrosocysteinereduction | 2.53 | 0.452 | 5.6 | pH 7, 30 °C | Open record: thioredoxin reductase (EhTRXR), S-nitrosocysteine |
| thioredoxin reductase (EhTRXR) | Entamoeba histolytica | S-nitrosocysteinereduction in the presence of 5 μM EcTRX | — | — | 1.2 | pH 7, 30 °C | Open record: thioredoxin reductase (EhTRXR), S-nitrosocysteine |
| thioredoxin reductase (EhTRXR) | Entamoeba histolytica | S-nitrosocysteinereduction in the presence of 5 μM EhTRX41 | — | — | 14 | pH 7, 30 °C | Open record: thioredoxin reductase (EhTRXR), S-nitrosocysteine |
| thioredoxin reductase (EhTRXR) | Entamoeba histolytica | S-nitrosoglutathionereduction | 0.38 | 0.342 | 1.1 | pH 7, 30 °C | Open record: thioredoxin reductase (EhTRXR), S-nitrosoglutathione |
| thioredoxin reductase (EhTRXR) | Entamoeba histolytica | S-nitrosoglutathionereduction in the presence of 5 μM EcTRX | — | — | 11 | pH 7, 30 °C | Open record: thioredoxin reductase (EhTRXR), S-nitrosoglutathione |
| thioredoxin reductase (EhTRXR) | Entamoeba histolytica | S-nitrosoglutathionereduction in the presence of 5 μM EhTRX41 | — | — | 11 | pH 7, 30 °C | Open record: thioredoxin reductase (EhTRXR), S-nitrosoglutathione |