Values CatLog read from this paper
Role of motif III in catalysis by acetyl-CoA synthetase
PMID 22973162doi:10.1155/2012/509579Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 13 records (17 values) from this paper: acetyl-CoA synthetase Acs1 from Methanothermobacter thermautotrophicus.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 4 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| acetyl-CoA synthetase Acs1 | Methanothermobacter thermautotrophicus | acetate | 66.6 | — | — | pH 7.5, 65 °C | Open record: acetyl-CoA synthetase Acs1, acetate |
| acetyl-CoA synthetase Acs1variant A500T | Methanothermobacter thermautotrophicus | acetate | 1.5 | 2.1 | 0.71 calc. | pH 7.5, 65 °C | Open record: acetyl-CoA synthetase Acs1 variant A500T, acetate |
| acetyl-CoA synthetase Acs1variant A500T | Methanothermobacter thermautotrophicus | adenosine 5'-triphosphate | — | 3.6 | — | pH 7.5, 65 °C | Open record: acetyl-CoA synthetase Acs1 variant A500T, adenosine 5'-triphosphate |
| acetyl-CoA synthetase Acs1variant A500T | Methanothermobacter thermautotrophicus | coenzyme A | — | 0.51 | — | pH 7.5, 65 °C | Open record: acetyl-CoA synthetase Acs1 variant A500T, coenzyme A |
| acetyl-CoA synthetase Acs1variant G501A | Methanothermobacter thermautotrophicus | acetate | 0.3 | 1.4 | 0.21 calc. | pH 7.5, 65 °C | Open record: acetyl-CoA synthetase Acs1 variant G501A, acetate |
| acetyl-CoA synthetase Acs1variant G501A | Methanothermobacter thermautotrophicus | adenosine 5'-triphosphate | — | 1.7 | — | pH 7.5, 65 °C | Open record: acetyl-CoA synthetase Acs1 variant G501A, adenosine 5'-triphosphate |
| acetyl-CoA synthetase Acs1variant G501A | Methanothermobacter thermautotrophicus | coenzyme A | — | 0.08 | — | pH 7.5, 65 °C | Open record: acetyl-CoA synthetase Acs1 variant G501A, coenzyme A |
| acetyl-CoA synthetase Acs1variant T499A | Methanothermobacter thermautotrophicus | acetate | 0.8 | 3 | 0.27 calc. | pH 7.5, 65 °C | Open record: acetyl-CoA synthetase Acs1 variant T499A, acetate |
| acetyl-CoA synthetase Acs1variant T499A | Methanothermobacter thermautotrophicus | adenosine 5'-triphosphate | — | 1.7 | — | pH 7.5, 65 °C | Open record: acetyl-CoA synthetase Acs1 variant T499A, adenosine 5'-triphosphate |
| acetyl-CoA synthetase Acs1variant T499A | Methanothermobacter thermautotrophicus | coenzyme A | — | 0.24 | — | pH 7.5, 65 °C | Open record: acetyl-CoA synthetase Acs1 variant T499A, coenzyme A |
| acetyl-CoA synthetase Acs1variant Y498A | Methanothermobacter thermautotrophicus | acetate | 1.6 | 7.5 | 0.21 calc. | pH 7.5, 65 °C | Open record: acetyl-CoA synthetase Acs1 variant Y498A, acetate |
| acetyl-CoA synthetase Acs1variant Y498A | Methanothermobacter thermautotrophicus | adenosine 5'-triphosphate | — | 1.7 | — | pH 7.5, 65 °C | Open record: acetyl-CoA synthetase Acs1 variant Y498A, adenosine 5'-triphosphate |
| acetyl-CoA synthetase Acs1variant Y498A | Methanothermobacter thermautotrophicus | coenzyme A | — | 0.1 | — | pH 7.5, 65 °C | Open record: acetyl-CoA synthetase Acs1 variant Y498A, coenzyme A |