Values CatLog read from this paper

Metal-ion dependent catalytic properties of Sulfolobus solfataricus class ii α-mannosidase

PMID 22989181doi:10.1021/bi301096aOpen in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 20 records (44 values) from this paper: class II α-mannosidase ManA from Sulfolobus solfataricus.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 2 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.

EC 3.2.1.24

Records read from this paper
Enzyme	Organism	Substrate	k_cat (s⁻¹)	K_m (mM)	k_cat/K_m (mM⁻¹ s⁻¹)	Conditions	Open record
class II α-mannosidase ManA	Sulfolobus solfataricus	2-O-alpha-D-mannopyranosyl-D-mannopyranoseEDTA-treated apo-ManA with 0.1 mM Co2+	5.5	0.85	6.4	pH 5, 70 °C	Open record: class II α-mannosidase ManA, 2-O-alpha-D-mannopyranosyl-D-mannopyranose
class II α-mannosidase ManA	Sulfolobus solfataricus	2-O-alpha-D-mannopyranosyl-D-mannopyranoseEDTA-treated apo-ManA with 0.1 mM Zn2+	—	—	0.206	pH 5, 70 °C	Open record: class II α-mannosidase ManA, 2-O-alpha-D-mannopyranosyl-D-mannopyranose
class II α-mannosidase ManA	Sulfolobus solfataricus	2-O-alpha-D-mannopyranosyl-D-mannopyranoseEDTA-treated apo-ManA with 0.1 mM Cd2+	—	—	0.277	pH 5, 70 °C	Open record: class II α-mannosidase ManA, 2-O-alpha-D-mannopyranosyl-D-mannopyranose
class II α-mannosidase ManA	Sulfolobus solfataricus	2-O-alpha-D-mannopyranosyl-D-mannopyranoseEDTA-treated apo-ManA with 0.1 mM Mn2+	3.1	2.3	1.3	pH 5, 70 °C	Open record: class II α-mannosidase ManA, 2-O-alpha-D-mannopyranosyl-D-mannopyranose
class II α-mannosidase ManA	Sulfolobus solfataricus	3-O-alpha-D-mannopyranosyl-D-mannopyranoseEDTA-treated apo-ManA with 0.1 mM Co2+	4.9	1.16	4.2	pH 5, 70 °C	Open record: class II α-mannosidase ManA, 3-O-alpha-D-mannopyranosyl-D-mannopyranose
class II α-mannosidase ManA	Sulfolobus solfataricus	3-O-alpha-D-mannopyranosyl-D-mannopyranoseEDTA-treated apo-ManA with 0.1 mM Zn2+	—	—	0.102	pH 5, 70 °C	Open record: class II α-mannosidase ManA, 3-O-alpha-D-mannopyranosyl-D-mannopyranose
class II α-mannosidase ManA	Sulfolobus solfataricus	3-O-alpha-D-mannopyranosyl-D-mannopyranoseEDTA-treated apo-ManA with 0.1 mM Cd2+	—	—	0.28	pH 5, 70 °C	Open record: class II α-mannosidase ManA, 3-O-alpha-D-mannopyranosyl-D-mannopyranose
class II α-mannosidase ManA	Sulfolobus solfataricus	3-O-alpha-D-mannopyranosyl-D-mannopyranoseEDTA-treated apo-ManA with 0.1 mM Mn2+	—	—	0.185	pH 5, 70 °C	Open record: class II α-mannosidase ManA, 3-O-alpha-D-mannopyranosyl-D-mannopyranose
class II α-mannosidase ManA	Sulfolobus solfataricus	4-nitrophenyl alpha-D-mannopyranosideuntreated enzyme (not EDTA-treated)	10.5	1	10.5	pH 5, 70 °C	Open record: class II α-mannosidase ManA, 4-nitrophenyl alpha-D-mannopyranoside
class II α-mannosidase ManA	Sulfolobus solfataricus	4-nitrophenyl alpha-D-mannopyranosideEDTA-treated apo-ManA with 0.1 mM Co2+	12.5	0.11	120	pH 5, 70 °C	Open record: class II α-mannosidase ManA, 4-nitrophenyl alpha-D-mannopyranoside
class II α-mannosidase ManA	Sulfolobus solfataricus	4-nitrophenyl alpha-D-mannopyranosideEDTA-treated apo-ManA with 0.1 mM Zn2+	13.6	0.65	21	pH 5, 70 °C	Open record: class II α-mannosidase ManA, 4-nitrophenyl alpha-D-mannopyranoside
class II α-mannosidase ManA	Sulfolobus solfataricus	4-nitrophenyl alpha-D-mannopyranosideEDTA-treated apo-ManA with 0.1 mM Cd2+	16.1	0.99	16	pH 5, 70 °C	Open record: class II α-mannosidase ManA, 4-nitrophenyl alpha-D-mannopyranoside
class II α-mannosidase ManA	Sulfolobus solfataricus	4-nitrophenyl alpha-D-mannopyranosideEDTA-treated apo-ManA with 0.1 mM Mn2+	4.8	0.39	12	pH 5, 70 °C	Open record: class II α-mannosidase ManA, 4-nitrophenyl alpha-D-mannopyranoside
class II α-mannosidase ManA	Sulfolobus solfataricus	4-nitrophenyl alpha-D-mannopyranosidepNP-mannoside and MnCl2 both varied	4.9	0.33	15 calc.	pH 5, 70 °C	Open record: class II α-mannosidase ManA, 4-nitrophenyl alpha-D-mannopyranoside
class II α-mannosidase ManA	Sulfolobus solfataricus	4-nitrophenyl alpha-D-mannopyranosidefixed 1.25 μM MnCl2	3.1	0.7	4.4 calc.	pH 5, 70 °C	Open record: class II α-mannosidase ManA, 4-nitrophenyl alpha-D-mannopyranoside
class II α-mannosidase ManA	Sulfolobus solfataricus	4-O-alpha-D-mannopyranosyl-D-mannopyranoseEDTA-treated apo-ManA with 0.1 mM Co2+	10.4	2.5	4.2	pH 5, 70 °C	Open record: class II α-mannosidase ManA, 4-O-alpha-D-mannopyranosyl-D-mannopyranose
class II α-mannosidase ManA	Sulfolobus solfataricus	4-O-alpha-D-mannopyranosyl-D-mannopyranoseEDTA-treated apo-ManA with 0.1 mM Zn2+	—	—	0.106	pH 5, 70 °C	Open record: class II α-mannosidase ManA, 4-O-alpha-D-mannopyranosyl-D-mannopyranose
class II α-mannosidase ManA	Sulfolobus solfataricus	4-O-alpha-D-mannopyranosyl-D-mannopyranoseEDTA-treated apo-ManA with 0.1 mM Cd2+	—	—	0.31	pH 5, 70 °C	Open record: class II α-mannosidase ManA, 4-O-alpha-D-mannopyranosyl-D-mannopyranose
class II α-mannosidase ManA	Sulfolobus solfataricus	4-O-alpha-D-mannopyranosyl-D-mannopyranoseEDTA-treated apo-ManA with 0.1 mM Mn2+	1.9	2	0.95	pH 5, 70 °C	Open record: class II α-mannosidase ManA, 4-O-alpha-D-mannopyranosyl-D-mannopyranose
class II α-mannosidase ManA	Sulfolobus solfataricus	6-O-alpha-D-mannopyranosyl-D-mannopyranose	2	2	1	pH 5, 70 °C	Open record: class II α-mannosidase ManA, 6-O-alpha-D-mannopyranosyl-D-mannopyranose