CatLogenzyme kinetics catalog

EC 3.2.1.24: alpha-mannosidase

HydrolasesOpen in Browse

CatLog read 47 records for EC 3.2.1.24 from 3 papers, for 2 organisms. Names in these papers: class II α-mannosidase (ManA), class II α-mannosidase ManA, alpha-D-mannosidase.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units.

CatLog also holds 48 records for EC 3.2.1.24 that are not yet re-read: from its earlier version, built from the enzyme databases and partly checked against the papers. All records for EC 3.2.1.24 in Browse.

Papers

PaperOrganismsRecords
Mutational analysis of divalent metal ion binding in the active site of class II α-mannosidase from Sulfolobus solfataricusSulfolobus solfataricus22
Metal-ion dependent catalytic properties of Sulfolobus solfataricus class ii α-mannosidaseSulfolobus solfataricus20
Effects of several alcohols on glycosidase activities in rat liver and brainRattus norvegicus5

Records

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)ConditionsPaper
alpha-D-mannosidaseRattus norvegicus4-nitrophenyl alpha-D-mannopyranosidecontrol rats (drank water only, same litter as treated rats)—13.35—pH 4.5, 37 °CPMID 1499277Open
alpha-D-mannosidaseRattus norvegicus4-nitrophenyl alpha-D-mannopyranoside—13.46—pH 4.5, 37 °CPMID 1499277Open
alpha-D-mannosidaseRattus norvegicus4-nitrophenyl alpha-D-mannopyranoside—13.6—pH 4.5, 37 °CPMID 1499277Open
alpha-D-mannosidaseRattus norvegicus4-nitrophenyl alpha-D-mannopyranoside—14.24—pH 4.5, 37 °CPMID 1499277Open
alpha-D-mannosidaseRattus norvegicus4-nitrophenyl alpha-D-mannopyranoside—12.24—pH 4.5, 37 °CPMID 1499277Open
class II α-mannosidase (ManA)Sulfolobus solfataricusp-nitrophenyl-α-mannoside1 mM CoCl211.30.3532.3pH 5, 70 °CPMID 25751413Open
class II α-mannosidase (ManA)Sulfolobus solfataricusp-nitrophenyl-α-mannoside1 mM ZnCl26.60.748.9pH 5, 70 °CPMID 25751413Open
class II α-mannosidase (ManA)Sulfolobus solfataricusp-nitrophenyl-α-mannoside4.80.4710.2pH 5, 70 °CPMID 25751413Open
class II α-mannosidase (ManA)Sulfolobus solfataricusp-nitrophenyl-α-mannoside1 mM CdCl215.31.98.1pH 5, 70 °CPMID 25751413Open
class II α-mannosidase (ManA)variant H228ESulfolobus solfataricusp-nitrophenyl-α-mannoside1 mM CoCl20.81.40.57pH 5, 70 °CPMID 25751413Open
class II α-mannosidase (ManA)variant H228ESulfolobus solfataricusp-nitrophenyl-α-mannoside1 mM ZnCl20.1181.10.11pH 5, 70 °CPMID 25751413Open
class II α-mannosidase (ManA)variant H228ESulfolobus solfataricusp-nitrophenyl-α-mannoside——0.019pH 5, 70 °CPMID 25751413Open
class II α-mannosidase (ManA)variant H228ESulfolobus solfataricusp-nitrophenyl-α-mannoside1 mM CdCl21.182.70.43pH 5, 70 °CPMID 25751413Open
class II α-mannosidase (ManA)variant H228QSulfolobus solfataricusp-nitrophenyl-α-mannoside1 mM CoCl21.61.70.94pH 5, 70 °CPMID 25751413Open
class II α-mannosidase (ManA)variant H228QSulfolobus solfataricusp-nitrophenyl-α-mannoside1 mM ZnCl20.62.50.24pH 5, 70 °CPMID 25751413Open
class II α-mannosidase (ManA)variant H228QSulfolobus solfataricusp-nitrophenyl-α-mannoside——0.038pH 5, 70 °CPMID 25751413Open
class II α-mannosidase (ManA)variant H228QSulfolobus solfataricusp-nitrophenyl-α-mannoside1 mM CdCl2——0.53pH 5, 70 °CPMID 25751413Open
class II α-mannosidase (ManA)variant H533ESulfolobus solfataricusp-nitrophenyl-α-mannoside1 mM CoCl24.32.91.5pH 5, 70 °CPMID 25751413Open
class II α-mannosidase (ManA)variant H533ESulfolobus solfataricusp-nitrophenyl-α-mannoside1 mM ZnCl2——0.0732pH 5, 70 °CPMID 25751413Open
class II α-mannosidase (ManA)variant H533ESulfolobus solfataricusp-nitrophenyl-α-mannoside0.1 mM ZnCl2——0.24pH 5, 70 °CPMID 25751413Open
class II α-mannosidase (ManA)variant H533ESulfolobus solfataricusp-nitrophenyl-α-mannoside——0.014pH 5, 70 °CPMID 25751413Open
class II α-mannosidase (ManA)variant H533ESulfolobus solfataricusp-nitrophenyl-α-mannoside1 mM CdCl2——0.046pH 5, 70 °CPMID 25751413Open
class II α-mannosidase (ManA)variant H533QSulfolobus solfataricusp-nitrophenyl-α-mannoside1 mM CoCl20.0990.130.76pH 5, 70 °CPMID 25751413Open
class II α-mannosidase (ManA)variant H533QSulfolobus solfataricusp-nitrophenyl-α-mannoside1 mM ZnCl21.421.90.75pH 5, 70 °CPMID 25751413Open
class II α-mannosidase (ManA)variant H533QSulfolobus solfataricusp-nitrophenyl-α-mannoside0.1 mM ZnCl21.61.11.5pH 5, 70 °CPMID 25751413Open
class II α-mannosidase (ManA)variant H533QSulfolobus solfataricusp-nitrophenyl-α-mannoside0.0880.70.13pH 5, 70 °CPMID 25751413Open
class II α-mannosidase (ManA)variant H533QSulfolobus solfataricusp-nitrophenyl-α-mannoside1 mM CdCl20.41.10.36pH 5, 70 °CPMID 25751413Open
class II α-mannosidase ManASulfolobus solfataricus2-O-alpha-D-mannopyranosyl-D-mannopyranoseEDTA-treated apo-ManA with 0.1 mM Co2+5.50.856.4pH 5, 70 °CPMID 22989181Open
class II α-mannosidase ManASulfolobus solfataricus2-O-alpha-D-mannopyranosyl-D-mannopyranoseEDTA-treated apo-ManA with 0.1 mM Zn2+——0.206pH 5, 70 °CPMID 22989181Open
class II α-mannosidase ManASulfolobus solfataricus2-O-alpha-D-mannopyranosyl-D-mannopyranoseEDTA-treated apo-ManA with 0.1 mM Cd2+——0.277pH 5, 70 °CPMID 22989181Open
class II α-mannosidase ManASulfolobus solfataricus2-O-alpha-D-mannopyranosyl-D-mannopyranoseEDTA-treated apo-ManA with 0.1 mM Mn2+3.12.31.3pH 5, 70 °CPMID 22989181Open
class II α-mannosidase ManASulfolobus solfataricus3-O-alpha-D-mannopyranosyl-D-mannopyranoseEDTA-treated apo-ManA with 0.1 mM Co2+4.91.164.2pH 5, 70 °CPMID 22989181Open
class II α-mannosidase ManASulfolobus solfataricus3-O-alpha-D-mannopyranosyl-D-mannopyranoseEDTA-treated apo-ManA with 0.1 mM Zn2+——0.102pH 5, 70 °CPMID 22989181Open
class II α-mannosidase ManASulfolobus solfataricus3-O-alpha-D-mannopyranosyl-D-mannopyranoseEDTA-treated apo-ManA with 0.1 mM Cd2+——0.28pH 5, 70 °CPMID 22989181Open
class II α-mannosidase ManASulfolobus solfataricus3-O-alpha-D-mannopyranosyl-D-mannopyranoseEDTA-treated apo-ManA with 0.1 mM Mn2+——0.185pH 5, 70 °CPMID 22989181Open
class II α-mannosidase ManASulfolobus solfataricus4-nitrophenyl alpha-D-mannopyranosideuntreated enzyme (not EDTA-treated)10.5110.5pH 5, 70 °CPMID 22989181Open
class II α-mannosidase ManASulfolobus solfataricus4-nitrophenyl alpha-D-mannopyranosideEDTA-treated apo-ManA with 0.1 mM Co2+12.50.11120pH 5, 70 °CPMID 22989181Open
class II α-mannosidase ManASulfolobus solfataricus4-nitrophenyl alpha-D-mannopyranosideEDTA-treated apo-ManA with 0.1 mM Zn2+13.60.6521pH 5, 70 °CPMID 22989181Open
class II α-mannosidase ManASulfolobus solfataricus4-nitrophenyl alpha-D-mannopyranosideEDTA-treated apo-ManA with 0.1 mM Cd2+16.10.9916pH 5, 70 °CPMID 22989181Open
class II α-mannosidase ManASulfolobus solfataricus4-nitrophenyl alpha-D-mannopyranosideEDTA-treated apo-ManA with 0.1 mM Mn2+4.80.3912pH 5, 70 °CPMID 22989181Open
class II α-mannosidase ManASulfolobus solfataricus4-nitrophenyl alpha-D-mannopyranosidepNP-mannoside and MnCl2 both varied4.90.3315 calc.pH 5, 70 °CPMID 22989181Open
class II α-mannosidase ManASulfolobus solfataricus4-nitrophenyl alpha-D-mannopyranosidefixed 1.25 μM MnCl23.10.74.4 calc.pH 5, 70 °CPMID 22989181Open
class II α-mannosidase ManASulfolobus solfataricus4-O-alpha-D-mannopyranosyl-D-mannopyranoseEDTA-treated apo-ManA with 0.1 mM Co2+10.42.54.2pH 5, 70 °CPMID 22989181Open
class II α-mannosidase ManASulfolobus solfataricus4-O-alpha-D-mannopyranosyl-D-mannopyranoseEDTA-treated apo-ManA with 0.1 mM Zn2+——0.106pH 5, 70 °CPMID 22989181Open
class II α-mannosidase ManASulfolobus solfataricus4-O-alpha-D-mannopyranosyl-D-mannopyranoseEDTA-treated apo-ManA with 0.1 mM Cd2+——0.31pH 5, 70 °CPMID 22989181Open
class II α-mannosidase ManASulfolobus solfataricus4-O-alpha-D-mannopyranosyl-D-mannopyranoseEDTA-treated apo-ManA with 0.1 mM Mn2+1.920.95pH 5, 70 °CPMID 22989181Open
class II α-mannosidase ManASulfolobus solfataricus6-O-alpha-D-mannopyranosyl-D-mannopyranose221pH 5, 70 °CPMID 22989181Open