Values CatLog read from this paper

Mutational analysis of divalent metal ion binding in the active site of class II α-mannosidase from Sulfolobus solfataricus

PMID 25751413doi:10.1021/acs.biochem.5b00090Open in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 22 records (52 values) from this paper: class II α-mannosidase (ManA) from Sulfolobus solfataricus.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.

EC 3.2.1.24

Records read from this paper
Enzyme	Organism	Substrate	k_cat (s⁻¹)	K_m (mM)	k_cat/K_m (mM⁻¹ s⁻¹)	Conditions	Open record
class II α-mannosidase (ManA)	Sulfolobus solfataricus	p-nitrophenyl-α-mannoside1 mM CoCl2	11.3	0.35	32.3	pH 5, 70 °C	Open record: class II α-mannosidase (ManA), p-nitrophenyl-α-mannoside
class II α-mannosidase (ManA)	Sulfolobus solfataricus	p-nitrophenyl-α-mannoside1 mM ZnCl2	6.6	0.74	8.9	pH 5, 70 °C	Open record: class II α-mannosidase (ManA), p-nitrophenyl-α-mannoside
class II α-mannosidase (ManA)	Sulfolobus solfataricus	p-nitrophenyl-α-mannoside	4.8	0.47	10.2	pH 5, 70 °C	Open record: class II α-mannosidase (ManA), p-nitrophenyl-α-mannoside
class II α-mannosidase (ManA)	Sulfolobus solfataricus	p-nitrophenyl-α-mannoside1 mM CdCl2	15.3	1.9	8.1	pH 5, 70 °C	Open record: class II α-mannosidase (ManA), p-nitrophenyl-α-mannoside
class II α-mannosidase (ManA)variant H228E	Sulfolobus solfataricus	p-nitrophenyl-α-mannoside1 mM CoCl2	0.8	1.4	0.57	pH 5, 70 °C	Open record: class II α-mannosidase (ManA) variant H228E, p-nitrophenyl-α-mannoside
class II α-mannosidase (ManA)variant H228E	Sulfolobus solfataricus	p-nitrophenyl-α-mannoside1 mM ZnCl2	0.118	1.1	0.11	pH 5, 70 °C	Open record: class II α-mannosidase (ManA) variant H228E, p-nitrophenyl-α-mannoside
class II α-mannosidase (ManA)variant H228E	Sulfolobus solfataricus	p-nitrophenyl-α-mannoside	—	—	0.019	pH 5, 70 °C	Open record: class II α-mannosidase (ManA) variant H228E, p-nitrophenyl-α-mannoside
class II α-mannosidase (ManA)variant H228E	Sulfolobus solfataricus	p-nitrophenyl-α-mannoside1 mM CdCl2	1.18	2.7	0.43	pH 5, 70 °C	Open record: class II α-mannosidase (ManA) variant H228E, p-nitrophenyl-α-mannoside
class II α-mannosidase (ManA)variant H228Q	Sulfolobus solfataricus	p-nitrophenyl-α-mannoside1 mM CoCl2	1.6	1.7	0.94	pH 5, 70 °C	Open record: class II α-mannosidase (ManA) variant H228Q, p-nitrophenyl-α-mannoside
class II α-mannosidase (ManA)variant H228Q	Sulfolobus solfataricus	p-nitrophenyl-α-mannoside1 mM ZnCl2	0.6	2.5	0.24	pH 5, 70 °C	Open record: class II α-mannosidase (ManA) variant H228Q, p-nitrophenyl-α-mannoside
class II α-mannosidase (ManA)variant H228Q	Sulfolobus solfataricus	p-nitrophenyl-α-mannoside	—	—	0.038	pH 5, 70 °C	Open record: class II α-mannosidase (ManA) variant H228Q, p-nitrophenyl-α-mannoside
class II α-mannosidase (ManA)variant H228Q	Sulfolobus solfataricus	p-nitrophenyl-α-mannoside1 mM CdCl2	—	—	0.53	pH 5, 70 °C	Open record: class II α-mannosidase (ManA) variant H228Q, p-nitrophenyl-α-mannoside
class II α-mannosidase (ManA)variant H533E	Sulfolobus solfataricus	p-nitrophenyl-α-mannoside1 mM CoCl2	4.3	2.9	1.5	pH 5, 70 °C	Open record: class II α-mannosidase (ManA) variant H533E, p-nitrophenyl-α-mannoside
class II α-mannosidase (ManA)variant H533E	Sulfolobus solfataricus	p-nitrophenyl-α-mannoside1 mM ZnCl2	—	—	0.0732	pH 5, 70 °C	Open record: class II α-mannosidase (ManA) variant H533E, p-nitrophenyl-α-mannoside
class II α-mannosidase (ManA)variant H533E	Sulfolobus solfataricus	p-nitrophenyl-α-mannoside0.1 mM ZnCl2	—	—	0.24	pH 5, 70 °C	Open record: class II α-mannosidase (ManA) variant H533E, p-nitrophenyl-α-mannoside
class II α-mannosidase (ManA)variant H533E	Sulfolobus solfataricus	p-nitrophenyl-α-mannoside	—	—	0.014	pH 5, 70 °C	Open record: class II α-mannosidase (ManA) variant H533E, p-nitrophenyl-α-mannoside
class II α-mannosidase (ManA)variant H533E	Sulfolobus solfataricus	p-nitrophenyl-α-mannoside1 mM CdCl2	—	—	0.046	pH 5, 70 °C	Open record: class II α-mannosidase (ManA) variant H533E, p-nitrophenyl-α-mannoside
class II α-mannosidase (ManA)variant H533Q	Sulfolobus solfataricus	p-nitrophenyl-α-mannoside1 mM CoCl2	0.099	0.13	0.76	pH 5, 70 °C	Open record: class II α-mannosidase (ManA) variant H533Q, p-nitrophenyl-α-mannoside
class II α-mannosidase (ManA)variant H533Q	Sulfolobus solfataricus	p-nitrophenyl-α-mannoside1 mM ZnCl2	1.42	1.9	0.75	pH 5, 70 °C	Open record: class II α-mannosidase (ManA) variant H533Q, p-nitrophenyl-α-mannoside
class II α-mannosidase (ManA)variant H533Q	Sulfolobus solfataricus	p-nitrophenyl-α-mannoside0.1 mM ZnCl2	1.6	1.1	1.5	pH 5, 70 °C	Open record: class II α-mannosidase (ManA) variant H533Q, p-nitrophenyl-α-mannoside
class II α-mannosidase (ManA)variant H533Q	Sulfolobus solfataricus	p-nitrophenyl-α-mannoside	0.088	0.7	0.13	pH 5, 70 °C	Open record: class II α-mannosidase (ManA) variant H533Q, p-nitrophenyl-α-mannoside
class II α-mannosidase (ManA)variant H533Q	Sulfolobus solfataricus	p-nitrophenyl-α-mannoside1 mM CdCl2	0.4	1.1	0.36	pH 5, 70 °C	Open record: class II α-mannosidase (ManA) variant H533Q, p-nitrophenyl-α-mannoside