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Role of key residues at the flavin mononucleotide (FMN):adenylyltransferase catalytic site of the bifunctional riboflavin kinase/flavin adenine dinucleotide (FAD) Synthetase from Corynebacterium…

PMID 23203077doi:10.3390/ijms131114492Open in Browse

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CatLog read 42 records (116 values) from this paper: bifunctional riboflavin kinase/FAD synthetase (CaFADS) from Corynebacterium ammoniagenes.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.

EC 2.7.1.26, EC 2.7.7.2

Records read from this paper
Enzyme	Organism	Substrate	k_cat (s⁻¹)	K_m (mM)	k_cat/K_m (mM⁻¹ s⁻¹)	Conditions	Open record
bifunctional riboflavin kinase/FAD synthetase (CaFADS)	Corynebacterium ammoniagenes	flavin adenine dinucleotide	0.22	0.0014	143	pH 7, 37 °C	Open record: bifunctional riboflavin kinase/FAD synthetase (CaFADS), flavin adenine dinucleotide
bifunctional riboflavin kinase/FAD synthetase (CaFADS)	Corynebacterium ammoniagenes	pyrophosphate	—	0.114	1.83	pH 7, 37 °C	Open record: bifunctional riboflavin kinase/FAD synthetase (CaFADS), pyrophosphate
bifunctional riboflavin kinase/FAD synthetase (CaFADS)variant H28A	Corynebacterium ammoniagenes	adenosine 5'-triphosphate	0.75	0.014	52	pH 7, 37 °C	Open record: bifunctional riboflavin kinase/FAD synthetase (CaFADS) variant H28A, adenosine 5'-triphosphate
bifunctional riboflavin kinase/FAD synthetase (CaFADS)variant H28A	Corynebacterium ammoniagenes	riboflavin	7.12	0.023	300	pH 7, 37 °C	Open record: bifunctional riboflavin kinase/FAD synthetase (CaFADS) variant H28A, riboflavin
bifunctional riboflavin kinase/FAD synthetase (CaFADS)variant H28D	Corynebacterium ammoniagenes	adenosine 5'-triphosphate	0.92	0.012	78	pH 7, 37 °C	Open record: bifunctional riboflavin kinase/FAD synthetase (CaFADS) variant H28D, adenosine 5'-triphosphate
bifunctional riboflavin kinase/FAD synthetase (CaFADS)variant H28D	Corynebacterium ammoniagenes	riboflavin	4.78	0.025	200	pH 7, 37 °C	Open record: bifunctional riboflavin kinase/FAD synthetase (CaFADS) variant H28D, riboflavin
bifunctional riboflavin kinase/FAD synthetase (CaFADS)variant H31A	Corynebacterium ammoniagenes	adenosine 5'-triphosphate	0.97	0.014	72	pH 7, 37 °C	Open record: bifunctional riboflavin kinase/FAD synthetase (CaFADS) variant H31A, adenosine 5'-triphosphate
bifunctional riboflavin kinase/FAD synthetase (CaFADS)variant H31A	Corynebacterium ammoniagenes	riboflavin	2.82	0.0045	620	pH 7, 37 °C	Open record: bifunctional riboflavin kinase/FAD synthetase (CaFADS) variant H31A, riboflavin
bifunctional riboflavin kinase/FAD synthetase (CaFADS)variant N125A	Corynebacterium ammoniagenes	adenosine 5'-triphosphate	0.77	0.024	32	pH 7, 37 °C	Open record: bifunctional riboflavin kinase/FAD synthetase (CaFADS) variant N125A, adenosine 5'-triphosphate
bifunctional riboflavin kinase/FAD synthetase (CaFADS)variant N125A	Corynebacterium ammoniagenes	riboflavin	1.85	0.0017	1.08 × 10³	pH 7, 37 °C	Open record: bifunctional riboflavin kinase/FAD synthetase (CaFADS) variant N125A, riboflavin
bifunctional riboflavin kinase/FAD synthetase (CaFADS)variant N125D	Corynebacterium ammoniagenes	adenosine 5'-triphosphate	1.3	0.035	38	pH 7, 37 °C	Open record: bifunctional riboflavin kinase/FAD synthetase (CaFADS) variant N125D, adenosine 5'-triphosphate
bifunctional riboflavin kinase/FAD synthetase (CaFADS)variant N125D	Corynebacterium ammoniagenes	flavin adenine dinucleotide	0.035	0.015	2.3	pH 7, 37 °C	Open record: bifunctional riboflavin kinase/FAD synthetase (CaFADS) variant N125D, flavin adenine dinucleotide
bifunctional riboflavin kinase/FAD synthetase (CaFADS)variant N125D	Corynebacterium ammoniagenes	pyrophosphate	—	0.32	0.12	pH 7, 37 °C	Open record: bifunctional riboflavin kinase/FAD synthetase (CaFADS) variant N125D, pyrophosphate
bifunctional riboflavin kinase/FAD synthetase (CaFADS)variant N125D	Corynebacterium ammoniagenes	riboflavin	6.92	0.016	430	pH 7, 37 °C	Open record: bifunctional riboflavin kinase/FAD synthetase (CaFADS) variant N125D, riboflavin
bifunctional riboflavin kinase/FAD synthetase (CaFADS)variant R161A	Corynebacterium ammoniagenes	adenosine 5'-triphosphate	—	0.001	250	pH 7, 37 °C	Open record: bifunctional riboflavin kinase/FAD synthetase (CaFADS) variant R161A, adenosine 5'-triphosphate
bifunctional riboflavin kinase/FAD synthetase (CaFADS)variant R161A	Corynebacterium ammoniagenes	adenosine 5'-triphosphate	1.08	0.012	93	pH 7, 37 °C	Open record: bifunctional riboflavin kinase/FAD synthetase (CaFADS) variant R161A, adenosine 5'-triphosphate
bifunctional riboflavin kinase/FAD synthetase (CaFADS)variant R161A	Corynebacterium ammoniagenes	flavin adenine dinucleotide	0.115	0.0017	73	pH 7, 37 °C	Open record: bifunctional riboflavin kinase/FAD synthetase (CaFADS) variant R161A, flavin adenine dinucleotide
bifunctional riboflavin kinase/FAD synthetase (CaFADS)variant R161A	Corynebacterium ammoniagenes	flavin mononucleotide	0.25	0.0061	40	pH 7, 37 °C	Open record: bifunctional riboflavin kinase/FAD synthetase (CaFADS) variant R161A, flavin mononucleotide
bifunctional riboflavin kinase/FAD synthetase (CaFADS)variant R161A	Corynebacterium ammoniagenes	pyrophosphate	—	0.215	0.53	pH 7, 37 °C	Open record: bifunctional riboflavin kinase/FAD synthetase (CaFADS) variant R161A, pyrophosphate
bifunctional riboflavin kinase/FAD synthetase (CaFADS)variant R161A	Corynebacterium ammoniagenes	riboflavin	5	0.012	420	pH 7, 37 °C	Open record: bifunctional riboflavin kinase/FAD synthetase (CaFADS) variant R161A, riboflavin
bifunctional riboflavin kinase/FAD synthetase (CaFADS)variant R161D	Corynebacterium ammoniagenes	adenosine 5'-triphosphate	—	0.0083	7.8	pH 7, 37 °C	Open record: bifunctional riboflavin kinase/FAD synthetase (CaFADS) variant R161D, adenosine 5'-triphosphate
bifunctional riboflavin kinase/FAD synthetase (CaFADS)variant R161D	Corynebacterium ammoniagenes	adenosine 5'-triphosphate	1.13	0.011	102	pH 7, 37 °C	Open record: bifunctional riboflavin kinase/FAD synthetase (CaFADS) variant R161D, adenosine 5'-triphosphate
bifunctional riboflavin kinase/FAD synthetase (CaFADS)variant R161D	Corynebacterium ammoniagenes	flavin adenine dinucleotide	0.035	0.017	2	pH 7, 37 °C	Open record: bifunctional riboflavin kinase/FAD synthetase (CaFADS) variant R161D, flavin adenine dinucleotide
bifunctional riboflavin kinase/FAD synthetase (CaFADS)variant R161D	Corynebacterium ammoniagenes	flavin mononucleotide	0.065	0.012	5.3	pH 7, 37 °C	Open record: bifunctional riboflavin kinase/FAD synthetase (CaFADS) variant R161D, flavin mononucleotide
bifunctional riboflavin kinase/FAD synthetase (CaFADS)variant R161D	Corynebacterium ammoniagenes	pyrophosphate	—	0.483	0.067	pH 7, 37 °C	Open record: bifunctional riboflavin kinase/FAD synthetase (CaFADS) variant R161D, pyrophosphate
bifunctional riboflavin kinase/FAD synthetase (CaFADS)variant R161D	Corynebacterium ammoniagenes	riboflavin	5	0.013	380	pH 7, 37 °C	Open record: bifunctional riboflavin kinase/FAD synthetase (CaFADS) variant R161D, riboflavin
bifunctional riboflavin kinase/FAD synthetase (CaFADS)variant S164A	Corynebacterium ammoniagenes	adenosine 5'-triphosphate	—	0.086	3.3	pH 7, 37 °C	Open record: bifunctional riboflavin kinase/FAD synthetase (CaFADS) variant S164A, adenosine 5'-triphosphate
bifunctional riboflavin kinase/FAD synthetase (CaFADS)variant S164A	Corynebacterium ammoniagenes	adenosine 5'-triphosphate	0.98	0.012	83	pH 7, 37 °C	Open record: bifunctional riboflavin kinase/FAD synthetase (CaFADS) variant S164A, adenosine 5'-triphosphate
bifunctional riboflavin kinase/FAD synthetase (CaFADS)variant S164A	Corynebacterium ammoniagenes	flavin adenine dinucleotide	0.028	0.017	1.7	pH 7, 37 °C	Open record: bifunctional riboflavin kinase/FAD synthetase (CaFADS) variant S164A, flavin adenine dinucleotide
bifunctional riboflavin kinase/FAD synthetase (CaFADS)variant S164A	Corynebacterium ammoniagenes	flavin mononucleotide	0.28	0.02	14	pH 7, 37 °C	Open record: bifunctional riboflavin kinase/FAD synthetase (CaFADS) variant S164A, flavin mononucleotide
bifunctional riboflavin kinase/FAD synthetase (CaFADS)variant S164A	Corynebacterium ammoniagenes	pyrophosphate	—	0.229	0.12	pH 7, 37 °C	Open record: bifunctional riboflavin kinase/FAD synthetase (CaFADS) variant S164A, pyrophosphate
bifunctional riboflavin kinase/FAD synthetase (CaFADS)variant S164A	Corynebacterium ammoniagenes	riboflavin	4.32	0.0088	480	pH 7, 37 °C	Open record: bifunctional riboflavin kinase/FAD synthetase (CaFADS) variant S164A, riboflavin
bifunctional riboflavin kinase/FAD synthetase (CaFADS)variant S164D	Corynebacterium ammoniagenes	adenosine 5'-triphosphate	0.92	0.01	88	pH 7, 37 °C	Open record: bifunctional riboflavin kinase/FAD synthetase (CaFADS) variant S164D, adenosine 5'-triphosphate
bifunctional riboflavin kinase/FAD synthetase (CaFADS)variant S164D	Corynebacterium ammoniagenes	riboflavin	3.08	0.0056	550	pH 7, 37 °C	Open record: bifunctional riboflavin kinase/FAD synthetase (CaFADS) variant S164D, riboflavin
bifunctional riboflavin kinase/FAD synthetase (CaFADS)variant T165A	Corynebacterium ammoniagenes	adenosine 5'-triphosphate	—	0.0023	57	pH 7, 37 °C	Open record: bifunctional riboflavin kinase/FAD synthetase (CaFADS) variant T165A, adenosine 5'-triphosphate
bifunctional riboflavin kinase/FAD synthetase (CaFADS)variant T165A	Corynebacterium ammoniagenes	adenosine 5'-triphosphate	1.1	0.012	90	pH 7, 37 °C	Open record: bifunctional riboflavin kinase/FAD synthetase (CaFADS) variant T165A, adenosine 5'-triphosphate
bifunctional riboflavin kinase/FAD synthetase (CaFADS)variant T165A	Corynebacterium ammoniagenes	flavin adenine dinucleotide	0.028	0.0013	20	pH 7, 37 °C	Open record: bifunctional riboflavin kinase/FAD synthetase (CaFADS) variant T165A, flavin adenine dinucleotide
bifunctional riboflavin kinase/FAD synthetase (CaFADS)variant T165A	Corynebacterium ammoniagenes	flavin mononucleotide	0.135	0.0021	63	pH 7, 37 °C	Open record: bifunctional riboflavin kinase/FAD synthetase (CaFADS) variant T165A, flavin mononucleotide
bifunctional riboflavin kinase/FAD synthetase (CaFADS)variant T165A	Corynebacterium ammoniagenes	pyrophosphate	—	0.537	0.05	pH 7, 37 °C	Open record: bifunctional riboflavin kinase/FAD synthetase (CaFADS) variant T165A, pyrophosphate
bifunctional riboflavin kinase/FAD synthetase (CaFADS)variant T165A	Corynebacterium ammoniagenes	riboflavin	2.85	0.01	280	pH 7, 37 °C	Open record: bifunctional riboflavin kinase/FAD synthetase (CaFADS) variant T165A, riboflavin
bifunctional riboflavin kinase/FAD synthetase (CaFADS)variant T165D	Corynebacterium ammoniagenes	adenosine 5'-triphosphate	0.75	0.011	68	pH 7, 37 °C	Open record: bifunctional riboflavin kinase/FAD synthetase (CaFADS) variant T165D, adenosine 5'-triphosphate
bifunctional riboflavin kinase/FAD synthetase (CaFADS)variant T165D	Corynebacterium ammoniagenes	riboflavin	3	0.0077	380	pH 7, 37 °C	Open record: bifunctional riboflavin kinase/FAD synthetase (CaFADS) variant T165D, riboflavin