EC 2.7.1.26: riboflavin kinase

TransferasesOpen in Browse

CatLog read 112 records for EC 2.7.1.26 from 6 papers, for 2 organisms. Names in these papers: bifunctional FAD synthetase (CaFADS), bifunctional riboflavin kinase/FAD synthetase (CaFADS), FAD synthase (CaFADS), FAD synthetase (CaFADS), riboflavin kinase (RFK) module and 4 more.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units.

CatLog also holds 22 records for EC 2.7.1.26 that are not yet re-read: from its earlier version, built from the enzyme databases and partly checked against the papers. All records for EC 2.7.1.26 in Browse.

Papers

Papers with records for EC 2.7.1.26
Paper	Organisms	Records
The trimer interface in the quaternary structure of the bifunctional prokaryotic FAD synthetase from Corynebacterium ammoniagenes	Corynebacterium ammoniagenes	48
Role of key residues at the flavin mononucleotide (FMN):adenylyltransferase catalytic site of the bifunctional riboflavin kinase/flavin adenine dinucleotide (FAD) Synthetase from Corynebacterium…	Corynebacterium ammoniagenes	22
Insights into the FMNAT Active Site of FAD Synthase: Aromaticity is Essential for Flavin Binding and Catalysis	Corynebacterium ammoniagenes	18
Kinetics and thermodynamics of the protein-ligand interactions in the riboflavin kinase activity of the FAD synthetase from Corynebacterium ammoniagenes	Corynebacterium ammoniagenes	12
An FMN hydrolase is fused to a riboflavin kinase homolog in plants	Arabidopsis thaliana	7
Quaternary organization in a bifunctional prokaryotic FAD synthetase: Involvement of an arginine at its adenylyltransferase module on the riboflavin kinase activity	Corynebacterium ammoniagenes	5

Records

Records for EC 2.7.1.26 read from the paper
Enzyme	Organism	Substrate	k_cat (s⁻¹)	K_m (mM)	k_cat/K_m (mM⁻¹ s⁻¹)	Conditions	Paper	Open record
AtFHy (N-terminal FMN hydrolase domain of AtFMN/FHy)	Arabidopsis thaliana	riboflavin 5'-phosphate (flavin mononucleotide)	0.822	8.05 × 10⁻⁴	1.02 × 10³	pH 7.5, 30 °C	PMID 16183635	Open record: AtFHy (N-terminal FMN hydrolase domain of AtFMN/FHy), riboflavin 5'-phosphate (flavin mononucleotide)
AtFMN (C-terminal riboflavin kinase domain of AtFMN/FHy)	Arabidopsis thaliana	adenosine 5'-triphosphate	0.138	0.00191	72.3	pH 7.5, 30 °C	PMID 16183635	Open record: AtFMN (C-terminal riboflavin kinase domain of AtFMN/FHy), adenosine 5'-triphosphate
AtFMN (C-terminal riboflavin kinase domain of AtFMN/FHy)	Arabidopsis thaliana	riboflavin	0.113	7.1 × 10⁻⁶	1.59 × 10⁴	pH 7.5, 30 °C	PMID 16183635	Open record: AtFMN (C-terminal riboflavin kinase domain of AtFMN/FHy), riboflavin
bifunctional FAD synthetase (CaFADS)variant D298A	Corynebacterium ammoniagenes	adenosine 5'-triphosphateRFK activity (RF + ATP -> FMN + ADP); 0.8 mM MgCl2; 1 min	0.537	0.0266	20	pH 7, 25 °C	PMID 28341845	Open record: bifunctional FAD synthetase (CaFADS) variant D298A, adenosine 5'-triphosphate
bifunctional FAD synthetase (CaFADS)variant D298A	Corynebacterium ammoniagenes	adenosine 5'-triphosphateFMNAT activity (FMN -> FAD); 10 mM MgCl2; FMN 0-15 µM	—	0.0207	5	pH 7, 25 °C	PMID 28341845	Open record: bifunctional FAD synthetase (CaFADS) variant D298A, adenosine 5'-triphosphate
bifunctional FAD synthetase (CaFADS)variant D298A	Corynebacterium ammoniagenes	flavin mononucleotide	0.102	0.0012	85	pH 7, 25 °C	PMID 28341845	Open record: bifunctional FAD synthetase (CaFADS) variant D298A, flavin mononucleotide
bifunctional FAD synthetase (CaFADS)variant D298A	Corynebacterium ammoniagenes	riboflavin	1.307	7 × 10⁻⁴	152	pH 7, 25 °C	PMID 28341845	Open record: bifunctional FAD synthetase (CaFADS) variant D298A, riboflavin
bifunctional FAD synthetase (CaFADS)variant D298E	Corynebacterium ammoniagenes	adenosine 5'-triphosphateRFK activity (RF + ATP -> FMN + ADP); 0.8 mM MgCl2; 1 min	0.348	0.0649	5.3	pH 7, 25 °C	PMID 28341845	Open record: bifunctional FAD synthetase (CaFADS) variant D298E, adenosine 5'-triphosphate
bifunctional FAD synthetase (CaFADS)variant D298E	Corynebacterium ammoniagenes	adenosine 5'-triphosphateFMNAT activity (FMN -> FAD); 10 mM MgCl2; FMN 0-15 µM	—	0.0104	5.2	pH 7, 25 °C	PMID 28341845	Open record: bifunctional FAD synthetase (CaFADS) variant D298E, adenosine 5'-triphosphate
bifunctional FAD synthetase (CaFADS)variant D298E	Corynebacterium ammoniagenes	flavin mononucleotide	0.055	9.5 × 10⁻⁴	57.8	pH 7, 25 °C	PMID 28341845	Open record: bifunctional FAD synthetase (CaFADS) variant D298E, flavin mononucleotide
bifunctional FAD synthetase (CaFADS)variant E203A	Corynebacterium ammoniagenes	adenosine 5'-triphosphateRFK activity (RF + ATP -> FMN + ADP); 0.8 mM MgCl2; 1 min	0.835	0.0892	9.3	pH 7, 25 °C	PMID 28341845	Open record: bifunctional FAD synthetase (CaFADS) variant E203A, adenosine 5'-triphosphate
bifunctional FAD synthetase (CaFADS)variant E203A	Corynebacterium ammoniagenes	adenosine 5'-triphosphateFMNAT activity (FMN -> FAD); 10 mM MgCl2; FMN 0-15 µM	—	0.0108	5	pH 7, 25 °C	PMID 28341845	Open record: bifunctional FAD synthetase (CaFADS) variant E203A, adenosine 5'-triphosphate
bifunctional FAD synthetase (CaFADS)variant E203A	Corynebacterium ammoniagenes	flavin mononucleotide	0.053	7 × 10⁻⁴	75	pH 7, 25 °C	PMID 28341845	Open record: bifunctional FAD synthetase (CaFADS) variant E203A, flavin mononucleotide
bifunctional FAD synthetase (CaFADS)variant E203A	Corynebacterium ammoniagenes	riboflavin	2.13	0.0037	577	pH 7, 25 °C	PMID 28341845	Open record: bifunctional FAD synthetase (CaFADS) variant E203A, riboflavin
bifunctional FAD synthetase (CaFADS)variant E301A	Corynebacterium ammoniagenes	adenosine 5'-triphosphateRFK activity (RF + ATP -> FMN + ADP); 0.8 mM MgCl2; 1 min	0.612	0.0309	20	pH 7, 25 °C	PMID 28341845	Open record: bifunctional FAD synthetase (CaFADS) variant E301A, adenosine 5'-triphosphate
bifunctional FAD synthetase (CaFADS)variant E301A	Corynebacterium ammoniagenes	adenosine 5'-triphosphateFMNAT activity (FMN -> FAD); 10 mM MgCl2; FMN 0-15 µM	—	0.0197	5.8	pH 7, 25 °C	PMID 28341845	Open record: bifunctional FAD synthetase (CaFADS) variant E301A, adenosine 5'-triphosphate
bifunctional FAD synthetase (CaFADS)variant E301A	Corynebacterium ammoniagenes	flavin mononucleotide	0.115	4.2 × 10⁻⁴	273	pH 7, 25 °C	PMID 28341845	Open record: bifunctional FAD synthetase (CaFADS) variant E301A, flavin mononucleotide
bifunctional FAD synthetase (CaFADS)variant E301A	Corynebacterium ammoniagenes	riboflavin	0.883	1 × 10⁻⁴	170	pH 7, 25 °C	PMID 28341845	Open record: bifunctional FAD synthetase (CaFADS) variant E301A, riboflavin
bifunctional FAD synthetase (CaFADS)variant E301K	Corynebacterium ammoniagenes	adenosine 5'-triphosphateRFK activity (RF + ATP -> FMN + ADP); 0.8 mM MgCl2; 1 min	0.998	0.0593	17	pH 7, 25 °C	PMID 28341845	Open record: bifunctional FAD synthetase (CaFADS) variant E301K, adenosine 5'-triphosphate
bifunctional FAD synthetase (CaFADS)variant E301K	Corynebacterium ammoniagenes	adenosine 5'-triphosphateFMNAT activity (FMN -> FAD); 10 mM MgCl2; FMN 0-15 µM	—	0.0153	6.8	pH 7, 25 °C	PMID 28341845	Open record: bifunctional FAD synthetase (CaFADS) variant E301K, adenosine 5'-triphosphate
bifunctional FAD synthetase (CaFADS)variant E301K	Corynebacterium ammoniagenes	flavin mononucleotide	0.105	8.5 × 10⁻⁴	123	pH 7, 25 °C	PMID 28341845	Open record: bifunctional FAD synthetase (CaFADS) variant E301K, flavin mononucleotide
bifunctional FAD synthetase (CaFADS)variant F206A	Corynebacterium ammoniagenes	adenosine 5'-triphosphateRFK activity (RF + ATP -> FMN + ADP); 0.8 mM MgCl2; 1 min	2.32	0.121	19	pH 7, 25 °C	PMID 28341845	Open record: bifunctional FAD synthetase (CaFADS) variant F206A, adenosine 5'-triphosphate
bifunctional FAD synthetase (CaFADS)variant F206A	Corynebacterium ammoniagenes	adenosine 5'-triphosphateFMNAT activity (FMN -> FAD); 10 mM MgCl2; FMN 0-15 µM	—	0.038	1.8	pH 7, 25 °C	PMID 28341845	Open record: bifunctional FAD synthetase (CaFADS) variant F206A, adenosine 5'-triphosphate
bifunctional FAD synthetase (CaFADS)variant F206A	Corynebacterium ammoniagenes	flavin mononucleotide	0.07	0.0029	24.2	pH 7, 25 °C	PMID 28341845	Open record: bifunctional FAD synthetase (CaFADS) variant F206A, flavin mononucleotide
bifunctional FAD synthetase (CaFADS)variant F206A	Corynebacterium ammoniagenes	riboflavin	3.15	0.0032	995	pH 7, 25 °C	PMID 28341845	Open record: bifunctional FAD synthetase (CaFADS) variant F206A, riboflavin
bifunctional FAD synthetase (CaFADS)variant F206K	Corynebacterium ammoniagenes	adenosine 5'-triphosphateRFK activity (RF + ATP -> FMN + ADP); 0.8 mM MgCl2; 1 min	2.5	0.14	17.8	pH 7, 25 °C	PMID 28341845	Open record: bifunctional FAD synthetase (CaFADS) variant F206K, adenosine 5'-triphosphate
bifunctional FAD synthetase (CaFADS)variant F206K	Corynebacterium ammoniagenes	adenosine 5'-triphosphateFMNAT activity (FMN -> FAD); 10 mM MgCl2; FMN 0-15 µM	—	0.0388	2.7	pH 7, 25 °C	PMID 28341845	Open record: bifunctional FAD synthetase (CaFADS) variant F206K, adenosine 5'-triphosphate
bifunctional FAD synthetase (CaFADS)variant F206K	Corynebacterium ammoniagenes	flavin mononucleotide	0.103	0.0054	19.2	pH 7, 25 °C	PMID 28341845	Open record: bifunctional FAD synthetase (CaFADS) variant F206K, flavin mononucleotide
bifunctional FAD synthetase (CaFADS)variant F206K	Corynebacterium ammoniagenes	riboflavin	2.93	0.004	740	pH 7, 25 °C	PMID 28341845	Open record: bifunctional FAD synthetase (CaFADS) variant F206K, riboflavin
bifunctional FAD synthetase (CaFADS)variant F206W	Corynebacterium ammoniagenes	adenosine 5'-triphosphateRFK activity (RF + ATP -> FMN + ADP); 0.8 mM MgCl2; 1 min	1.17	0.0247	47.3	pH 7, 25 °C	PMID 28341845	Open record: bifunctional FAD synthetase (CaFADS) variant F206W, adenosine 5'-triphosphate
bifunctional FAD synthetase (CaFADS)variant F206W	Corynebacterium ammoniagenes	adenosine 5'-triphosphateFMNAT activity (FMN -> FAD); 10 mM MgCl2; FMN 0-15 µM	—	0.0252	3.7	pH 7, 25 °C	PMID 28341845	Open record: bifunctional FAD synthetase (CaFADS) variant F206W, adenosine 5'-triphosphate
bifunctional FAD synthetase (CaFADS)variant F206W	Corynebacterium ammoniagenes	flavin mononucleotide	0.077	0.0017	55	pH 7, 25 °C	PMID 28341845	Open record: bifunctional FAD synthetase (CaFADS) variant F206W, flavin mononucleotide
bifunctional FAD synthetase (CaFADS)variant K202A	Corynebacterium ammoniagenes	adenosine 5'-triphosphateRFK activity (RF + ATP -> FMN + ADP); 0.8 mM MgCl2; 1 min	0.575	0.0527	11	pH 7, 25 °C	PMID 28341845	Open record: bifunctional FAD synthetase (CaFADS) variant K202A, adenosine 5'-triphosphate
bifunctional FAD synthetase (CaFADS)variant K202A	Corynebacterium ammoniagenes	adenosine 5'-triphosphateFMNAT activity (FMN -> FAD); 10 mM MgCl2; FMN 0-15 µM	—	0.0121	3.2	pH 7, 25 °C	PMID 28341845	Open record: bifunctional FAD synthetase (CaFADS) variant K202A, adenosine 5'-triphosphate
bifunctional FAD synthetase (CaFADS)variant K202A	Corynebacterium ammoniagenes	flavin mononucleotide	0.038	0.0029	13.3	pH 7, 25 °C	PMID 28341845	Open record: bifunctional FAD synthetase (CaFADS) variant K202A, flavin mononucleotide
bifunctional FAD synthetase (CaFADS)variant K202A	Corynebacterium ammoniagenes	riboflavin	1.063	8 × 10⁻⁴	165	pH 7, 25 °C	PMID 28341845	Open record: bifunctional FAD synthetase (CaFADS) variant K202A, riboflavin
bifunctional FAD synthetase (CaFADS)variant L304A	Corynebacterium ammoniagenes	adenosine 5'-triphosphateRFK activity (RF + ATP -> FMN + ADP); 0.8 mM MgCl2; 1 min	2.95	0.004	738	pH 7, 25 °C	PMID 28341845	Open record: bifunctional FAD synthetase (CaFADS) variant L304A, adenosine 5'-triphosphate
bifunctional FAD synthetase (CaFADS)variant L304A	Corynebacterium ammoniagenes	adenosine 5'-triphosphateFMNAT activity (FMN -> FAD); 10 mM MgCl2; FMN 0-15 µM	—	0.0347	1.2	pH 7, 25 °C	PMID 28341845	Open record: bifunctional FAD synthetase (CaFADS) variant L304A, adenosine 5'-triphosphate
bifunctional FAD synthetase (CaFADS)variant L304A	Corynebacterium ammoniagenes	flavin mononucleotide	0.042	0.0028	15.2	pH 7, 25 °C	PMID 28341845	Open record: bifunctional FAD synthetase (CaFADS) variant L304A, flavin mononucleotide
bifunctional FAD synthetase (CaFADS)variant L304A	Corynebacterium ammoniagenes	riboflavin	4.33	0.0033	1313	pH 7, 25 °C	PMID 28341845	Open record: bifunctional FAD synthetase (CaFADS) variant L304A, riboflavin
bifunctional FAD synthetase (CaFADS)variant L304K	Corynebacterium ammoniagenes	adenosine 5'-triphosphateRFK activity (RF + ATP -> FMN + ADP); 0.8 mM MgCl2; 1 min	2.18	0.0048	457	pH 7, 25 °C	PMID 28341845	Open record: bifunctional FAD synthetase (CaFADS) variant L304K, adenosine 5'-triphosphate
bifunctional FAD synthetase (CaFADS)variant L304K	Corynebacterium ammoniagenes	adenosine 5'-triphosphateFMNAT activity (FMN -> FAD); 10 mM MgCl2; FMN 0-15 µM	—	0.0115	7.5	pH 7, 25 °C	PMID 28341845	Open record: bifunctional FAD synthetase (CaFADS) variant L304K, adenosine 5'-triphosphate
bifunctional FAD synthetase (CaFADS)variant L304K	Corynebacterium ammoniagenes	flavin mononucleotide	0.087	0.0152	5.7	pH 7, 25 °C	PMID 28341845	Open record: bifunctional FAD synthetase (CaFADS) variant L304K, flavin mononucleotide
bifunctional FAD synthetase (CaFADS)variant L304K	Corynebacterium ammoniagenes	riboflavin	9	0.0149	607	pH 7, 25 °C	PMID 28341845	Open record: bifunctional FAD synthetase (CaFADS) variant L304K, riboflavin
bifunctional FAD synthetase (CaFADS)variant V300A	Corynebacterium ammoniagenes	adenosine 5'-triphosphateRFK activity (RF + ATP -> FMN + ADP); 0.8 mM MgCl2; 1 min	1.162	0.0375	31	pH 7, 25 °C	PMID 28341845	Open record: bifunctional FAD synthetase (CaFADS) variant V300A, adenosine 5'-triphosphate
bifunctional FAD synthetase (CaFADS)variant V300A	Corynebacterium ammoniagenes	adenosine 5'-triphosphateFMNAT activity (FMN -> FAD); 10 mM MgCl2; FMN 0-15 µM	—	0.0462	1.8	pH 7, 25 °C	PMID 28341845	Open record: bifunctional FAD synthetase (CaFADS) variant V300A, adenosine 5'-triphosphate
bifunctional FAD synthetase (CaFADS)variant V300A	Corynebacterium ammoniagenes	flavin mononucleotide	0.082	0.0014	58	pH 7, 25 °C	PMID 28341845	Open record: bifunctional FAD synthetase (CaFADS) variant V300A, flavin mononucleotide
bifunctional FAD synthetase (CaFADS)variant V300K	Corynebacterium ammoniagenes	adenosine 5'-triphosphateRFK activity (RF + ATP -> FMN + ADP); 0.8 mM MgCl2; 1 min	3.12	0.00725	430	pH 7, 25 °C	PMID 28341845	Open record: bifunctional FAD synthetase (CaFADS) variant V300K, adenosine 5'-triphosphate
bifunctional FAD synthetase (CaFADS)variant V300K	Corynebacterium ammoniagenes	adenosine 5'-triphosphateFMNAT activity (FMN -> FAD); 10 mM MgCl2; FMN 0-15 µM	—	0.0095	8	pH 7, 25 °C	PMID 28341845	Open record: bifunctional FAD synthetase (CaFADS) variant V300K, adenosine 5'-triphosphate
bifunctional FAD synthetase (CaFADS)variant V300K	Corynebacterium ammoniagenes	flavin mononucleotide	0.077	0.0083	9.2	pH 7, 25 °C	PMID 28341845	Open record: bifunctional FAD synthetase (CaFADS) variant V300K, flavin mononucleotide
bifunctional FAD synthetase (CaFADS)variant V300K	Corynebacterium ammoniagenes	riboflavin	5.98	0.0097	613	pH 7, 25 °C	PMID 28341845	Open record: bifunctional FAD synthetase (CaFADS) variant V300K, riboflavin
bifunctional riboflavin kinase/FAD synthetase (CaFADS)variant H28A	Corynebacterium ammoniagenes	adenosine 5'-triphosphate	0.75	0.014	52	pH 7, 37 °C	PMID 23203077	Open record: bifunctional riboflavin kinase/FAD synthetase (CaFADS) variant H28A, adenosine 5'-triphosphate
bifunctional riboflavin kinase/FAD synthetase (CaFADS)variant H28A	Corynebacterium ammoniagenes	riboflavin	7.12	0.023	300	pH 7, 37 °C	PMID 23203077	Open record: bifunctional riboflavin kinase/FAD synthetase (CaFADS) variant H28A, riboflavin
bifunctional riboflavin kinase/FAD synthetase (CaFADS)variant H28D	Corynebacterium ammoniagenes	adenosine 5'-triphosphate	0.92	0.012	78	pH 7, 37 °C	PMID 23203077	Open record: bifunctional riboflavin kinase/FAD synthetase (CaFADS) variant H28D, adenosine 5'-triphosphate
bifunctional riboflavin kinase/FAD synthetase (CaFADS)variant H28D	Corynebacterium ammoniagenes	riboflavin	4.78	0.025	200	pH 7, 37 °C	PMID 23203077	Open record: bifunctional riboflavin kinase/FAD synthetase (CaFADS) variant H28D, riboflavin
bifunctional riboflavin kinase/FAD synthetase (CaFADS)variant H31A	Corynebacterium ammoniagenes	adenosine 5'-triphosphate	0.97	0.014	72	pH 7, 37 °C	PMID 23203077	Open record: bifunctional riboflavin kinase/FAD synthetase (CaFADS) variant H31A, adenosine 5'-triphosphate
bifunctional riboflavin kinase/FAD synthetase (CaFADS)variant H31A	Corynebacterium ammoniagenes	riboflavin	2.82	0.0045	620	pH 7, 37 °C	PMID 23203077	Open record: bifunctional riboflavin kinase/FAD synthetase (CaFADS) variant H31A, riboflavin
bifunctional riboflavin kinase/FAD synthetase (CaFADS)variant N125A	Corynebacterium ammoniagenes	adenosine 5'-triphosphate	0.77	0.024	32	pH 7, 37 °C	PMID 23203077	Open record: bifunctional riboflavin kinase/FAD synthetase (CaFADS) variant N125A, adenosine 5'-triphosphate
bifunctional riboflavin kinase/FAD synthetase (CaFADS)variant N125A	Corynebacterium ammoniagenes	riboflavin	1.85	0.0017	1.08 × 10³	pH 7, 37 °C	PMID 23203077	Open record: bifunctional riboflavin kinase/FAD synthetase (CaFADS) variant N125A, riboflavin
bifunctional riboflavin kinase/FAD synthetase (CaFADS)variant N125D	Corynebacterium ammoniagenes	adenosine 5'-triphosphate	1.3	0.035	38	pH 7, 37 °C	PMID 23203077	Open record: bifunctional riboflavin kinase/FAD synthetase (CaFADS) variant N125D, adenosine 5'-triphosphate
bifunctional riboflavin kinase/FAD synthetase (CaFADS)variant N125D	Corynebacterium ammoniagenes	riboflavin	6.92	0.016	430	pH 7, 37 °C	PMID 23203077	Open record: bifunctional riboflavin kinase/FAD synthetase (CaFADS) variant N125D, riboflavin
bifunctional riboflavin kinase/FAD synthetase (CaFADS)variant R161A	Corynebacterium ammoniagenes	adenosine 5'-triphosphate	1.08	0.012	93	pH 7, 37 °C	PMID 23203077	Open record: bifunctional riboflavin kinase/FAD synthetase (CaFADS) variant R161A, adenosine 5'-triphosphate
bifunctional riboflavin kinase/FAD synthetase (CaFADS)variant R161A	Corynebacterium ammoniagenes	riboflavin	5	0.012	420	pH 7, 37 °C	PMID 23203077	Open record: bifunctional riboflavin kinase/FAD synthetase (CaFADS) variant R161A, riboflavin
bifunctional riboflavin kinase/FAD synthetase (CaFADS)variant R161D	Corynebacterium ammoniagenes	adenosine 5'-triphosphate	1.13	0.011	102	pH 7, 37 °C	PMID 23203077	Open record: bifunctional riboflavin kinase/FAD synthetase (CaFADS) variant R161D, adenosine 5'-triphosphate
bifunctional riboflavin kinase/FAD synthetase (CaFADS)variant R161D	Corynebacterium ammoniagenes	riboflavin	5	0.013	380	pH 7, 37 °C	PMID 23203077	Open record: bifunctional riboflavin kinase/FAD synthetase (CaFADS) variant R161D, riboflavin
bifunctional riboflavin kinase/FAD synthetase (CaFADS)variant S164A	Corynebacterium ammoniagenes	adenosine 5'-triphosphate	0.98	0.012	83	pH 7, 37 °C	PMID 23203077	Open record: bifunctional riboflavin kinase/FAD synthetase (CaFADS) variant S164A, adenosine 5'-triphosphate
bifunctional riboflavin kinase/FAD synthetase (CaFADS)variant S164A	Corynebacterium ammoniagenes	riboflavin	4.32	0.0088	480	pH 7, 37 °C	PMID 23203077	Open record: bifunctional riboflavin kinase/FAD synthetase (CaFADS) variant S164A, riboflavin
bifunctional riboflavin kinase/FAD synthetase (CaFADS)variant S164D	Corynebacterium ammoniagenes	adenosine 5'-triphosphate	0.92	0.01	88	pH 7, 37 °C	PMID 23203077	Open record: bifunctional riboflavin kinase/FAD synthetase (CaFADS) variant S164D, adenosine 5'-triphosphate
bifunctional riboflavin kinase/FAD synthetase (CaFADS)variant S164D	Corynebacterium ammoniagenes	riboflavin	3.08	0.0056	550	pH 7, 37 °C	PMID 23203077	Open record: bifunctional riboflavin kinase/FAD synthetase (CaFADS) variant S164D, riboflavin
bifunctional riboflavin kinase/FAD synthetase (CaFADS)variant T165A	Corynebacterium ammoniagenes	adenosine 5'-triphosphate	1.1	0.012	90	pH 7, 37 °C	PMID 23203077	Open record: bifunctional riboflavin kinase/FAD synthetase (CaFADS) variant T165A, adenosine 5'-triphosphate
bifunctional riboflavin kinase/FAD synthetase (CaFADS)variant T165A	Corynebacterium ammoniagenes	riboflavin	2.85	0.01	280	pH 7, 37 °C	PMID 23203077	Open record: bifunctional riboflavin kinase/FAD synthetase (CaFADS) variant T165A, riboflavin
bifunctional riboflavin kinase/FAD synthetase (CaFADS)variant T165D	Corynebacterium ammoniagenes	adenosine 5'-triphosphate	0.75	0.011	68	pH 7, 37 °C	PMID 23203077	Open record: bifunctional riboflavin kinase/FAD synthetase (CaFADS) variant T165D, adenosine 5'-triphosphate
bifunctional riboflavin kinase/FAD synthetase (CaFADS)variant T165D	Corynebacterium ammoniagenes	riboflavin	3	0.0077	380	pH 7, 37 °C	PMID 23203077	Open record: bifunctional riboflavin kinase/FAD synthetase (CaFADS) variant T165D, riboflavin
FAD synthase (CaFADS)variant F128A	Corynebacterium ammoniagenes	adenosine 5'-triphosphate	0.58	0.03	20	pH 7, 25 °C	PMID 32466340	Open record: FAD synthase (CaFADS) variant F128A, adenosine 5'-triphosphate
FAD synthase (CaFADS)variant F128A	Corynebacterium ammoniagenes	riboflavin	1.75	0.0026	670	pH 7, 25 °C	PMID 32466340	Open record: FAD synthase (CaFADS) variant F128A, riboflavin
FAD synthase (CaFADS)variant F128K	Corynebacterium ammoniagenes	adenosine 5'-triphosphate	0.85	0.03	28	pH 7, 25 °C	PMID 32466340	Open record: FAD synthase (CaFADS) variant F128K, adenosine 5'-triphosphate
FAD synthase (CaFADS)variant F128K	Corynebacterium ammoniagenes	riboflavin	1.13	0.0017	670	pH 7, 25 °C	PMID 32466340	Open record: FAD synthase (CaFADS) variant F128K, riboflavin
FAD synthase (CaFADS)variant F128W	Corynebacterium ammoniagenes	adenosine 5'-triphosphate	1.17	0.038	32	pH 7, 25 °C	PMID 32466340	Open record: FAD synthase (CaFADS) variant F128W, adenosine 5'-triphosphate
FAD synthase (CaFADS)variant F128W	Corynebacterium ammoniagenes	riboflavin	2.08	0.0043	480	pH 7, 25 °C	PMID 32466340	Open record: FAD synthase (CaFADS) variant F128W, riboflavin
FAD synthase (CaFADS)variant F62A	Corynebacterium ammoniagenes	adenosine 5'-triphosphate	2.12	0.014	155	pH 7, 25 °C	PMID 32466340	Open record: FAD synthase (CaFADS) variant F62A, adenosine 5'-triphosphate
FAD synthase (CaFADS)variant F62A	Corynebacterium ammoniagenes	riboflavin	4.73	0.0025	1.858 × 10⁴	pH 7, 25 °C	PMID 32466340	Open record: FAD synthase (CaFADS) variant F62A, riboflavin
FAD synthase (CaFADS)variant F62K	Corynebacterium ammoniagenes	adenosine 5'-triphosphate	2.3	0.018	127	pH 7, 25 °C	PMID 32466340	Open record: FAD synthase (CaFADS) variant F62K, adenosine 5'-triphosphate
FAD synthase (CaFADS)variant F62K	Corynebacterium ammoniagenes	riboflavin	2.18	2 × 10⁻⁴	8.9 × 10³	pH 7, 25 °C	PMID 32466340	Open record: FAD synthase (CaFADS) variant F62K, riboflavin
FAD synthase (CaFADS)variant F62W	Corynebacterium ammoniagenes	adenosine 5'-triphosphate	1.53	0.018	87	pH 7, 25 °C	PMID 32466340	Open record: FAD synthase (CaFADS) variant F62W, adenosine 5'-triphosphate
FAD synthase (CaFADS)variant F62W	Corynebacterium ammoniagenes	riboflavin	2.52	0.0044	570	pH 7, 25 °C	PMID 32466340	Open record: FAD synthase (CaFADS) variant F62W, riboflavin
FAD synthase (CaFADS)variant Y106A	Corynebacterium ammoniagenes	adenosine 5'-triphosphate	0.88	0.029	30	pH 7, 25 °C	PMID 32466340	Open record: FAD synthase (CaFADS) variant Y106A, adenosine 5'-triphosphate
FAD synthase (CaFADS)variant Y106A	Corynebacterium ammoniagenes	riboflavin	2.38	0.0025	970	pH 7, 25 °C	PMID 32466340	Open record: FAD synthase (CaFADS) variant Y106A, riboflavin
FAD synthase (CaFADS)variant Y106K	Corynebacterium ammoniagenes	adenosine 5'-triphosphate	0.9	0.039	23	pH 7, 25 °C	PMID 32466340	Open record: FAD synthase (CaFADS) variant Y106K, adenosine 5'-triphosphate
FAD synthase (CaFADS)variant Y106K	Corynebacterium ammoniagenes	riboflavin	2.47	0.0021	1.18 × 10³	pH 7, 25 °C	PMID 32466340	Open record: FAD synthase (CaFADS) variant Y106K, riboflavin
FAD synthase (CaFADS)variant Y106W	Corynebacterium ammoniagenes	adenosine 5'-triphosphate	0.35	0.049	6.7	pH 7, 25 °C	PMID 32466340	Open record: FAD synthase (CaFADS) variant Y106W, adenosine 5'-triphosphate
FAD synthase (CaFADS)variant Y106W	Corynebacterium ammoniagenes	riboflavin	0.7	6 × 10⁻⁴	1.13 × 10³	pH 7, 25 °C	PMID 32466340	Open record: FAD synthase (CaFADS) variant Y106W, riboflavin
FAD synthetase (CaFADS), riboflavin kinase (RFK) activity	Corynebacterium ammoniagenes	adenosine 5'-triphosphate	2.592	0.0282	92	pH 7, 25 °C	PMID 25801930	Open record: FAD synthetase (CaFADS), riboflavin kinase (RFK) activity, adenosine 5'-triphosphate
FAD synthetase (CaFADS), riboflavin kinase (RFK) activity	Corynebacterium ammoniagenes	riboflavin	6.802	0.0117	582	pH 7, 25 °C	PMID 25801930	Open record: FAD synthetase (CaFADS), riboflavin kinase (RFK) activity, riboflavin
FAD synthetase (CaFADS), riboflavin kinase (RFK) activityvariant R66A	Corynebacterium ammoniagenes	adenosine 5'-triphosphate	0.182	0.087	2.2	pH 7, 25 °C	PMID 25801930	Open record: FAD synthetase (CaFADS), riboflavin kinase (RFK) activity variant R66A, adenosine 5'-triphosphate
FAD synthetase (CaFADS), riboflavin kinase (RFK) activityvariant R66E	Corynebacterium ammoniagenes	adenosine 5'-triphosphate	0.397	0.0215	18	pH 7, 25 °C	PMID 25801930	Open record: FAD synthetase (CaFADS), riboflavin kinase (RFK) activity variant R66E, adenosine 5'-triphosphate
FAD synthetase (CaFADS), riboflavin kinase (RFK) activityvariant R66E	Corynebacterium ammoniagenes	riboflavin	0.702	8.9 × 10⁻⁴	788	pH 7, 25 °C	PMID 25801930	Open record: FAD synthetase (CaFADS), riboflavin kinase (RFK) activity variant R66E, riboflavin
FAD synthetase (CaFADS), riboflavin kinase (RFK) module	Corynebacterium ammoniagenes	adenosine 5'-triphosphateno product added; Michaelis-Menten fit	2.7	0.06	44 calc.	pH 7, 25 °C	PMID 28779158	Open record: FAD synthetase (CaFADS), riboflavin kinase (RFK) module, adenosine 5'-triphosphate
FAD synthetase (CaFADS), riboflavin kinase (RFK) module	Corynebacterium ammoniagenes	adenosine 5'-triphosphateATP varied (10–350 µM) at ~5 µM RF; Michaelis-Menten fit	2.7	0.11	24 calc.	pH 7, 25 °C	PMID 28779158	Open record: FAD synthetase (CaFADS), riboflavin kinase (RFK) module, adenosine 5'-triphosphate
FAD synthetase (CaFADS), riboflavin kinase (RFK) module	Corynebacterium ammoniagenes	adenosine 5'-triphosphateATP varied (10–350 µM) at ~5 µM RF; Michaelis-Menten fit	2.3	0.14	17 calc.	pH 7, 25 °C	PMID 28779158	Open record: FAD synthetase (CaFADS), riboflavin kinase (RFK) module, adenosine 5'-triphosphate
FAD synthetase (CaFADS), riboflavin kinase (RFK) module	Corynebacterium ammoniagenes	adenosine 5'-triphosphateATP varied (10–350 µM) at ~5 µM RF; Michaelis-Menten fit	2.8	0.26	11 calc.	pH 7, 25 °C	PMID 28779158	Open record: FAD synthetase (CaFADS), riboflavin kinase (RFK) module, adenosine 5'-triphosphate
FAD synthetase (CaFADS), riboflavin kinase (RFK) module	Corynebacterium ammoniagenes	adenosine 5'-triphosphateATP varied (10–350 µM) at ~5 µM RF; Michaelis-Menten fit	2.8	0.7	4.0 calc.	pH 7, 25 °C	PMID 28779158	Open record: FAD synthetase (CaFADS), riboflavin kinase (RFK) module, adenosine 5'-triphosphate
FAD synthetase (CaFADS), riboflavin kinase (RFK) module	Corynebacterium ammoniagenes	adenosine 5'-triphosphateATP varied at ~5 µM RF	2.2	0.04	54 calc.	pH 7, 25 °C	PMID 28779158	Open record: FAD synthetase (CaFADS), riboflavin kinase (RFK) module, adenosine 5'-triphosphate
FAD synthetase (CaFADS), riboflavin kinase (RFK) module	Corynebacterium ammoniagenes	riboflavinRF varied (0.5–45 µM) at saturating ATP (350 µM)	7.3	0.01	730 calc.	pH 7, 25 °C	PMID 28779158	Open record: FAD synthetase (CaFADS), riboflavin kinase (RFK) module, riboflavin
FAD synthetase (CaFADS), riboflavin kinase (RFK) module	Corynebacterium ammoniagenes	riboflavinRF varied (up to 7 µM) at saturating ATP (350 µM)	4.7	0.005	930 calc.	pH 7, 25 °C	PMID 28779158	Open record: FAD synthetase (CaFADS), riboflavin kinase (RFK) module, riboflavin
FAD synthetase (CaFADS), riboflavin kinase (RFK) module	Corynebacterium ammoniagenes	riboflavinRF varied (up to 7 µM) at saturating ATP (350 µM)	4.5	0.0077	580 calc.	pH 7, 25 °C	PMID 28779158	Open record: FAD synthetase (CaFADS), riboflavin kinase (RFK) module, riboflavin
FAD synthetase (CaFADS), riboflavin kinase (RFK) module	Corynebacterium ammoniagenes	riboflavinRF varied (up to 7 µM) at saturating ATP (350 µM)	2.3	0.0036	650 calc.	pH 7, 25 °C	PMID 28779158	Open record: FAD synthetase (CaFADS), riboflavin kinase (RFK) module, riboflavin
FAD synthetase (CaFADS), riboflavin kinase (RFK) module	Corynebacterium ammoniagenes	riboflavinRF varied (up to 7 µM) at saturating ATP (350 µM)	2	0.0055	360 calc.	pH 7, 25 °C	PMID 28779158	Open record: FAD synthetase (CaFADS), riboflavin kinase (RFK) module, riboflavin
FAD synthetase (CaFADS), riboflavin kinase (RFK) module	Corynebacterium ammoniagenes	riboflavinRF varied at saturating ATP (350 µM)	5.3	0.0069	770 calc.	pH 7, 25 °C	PMID 28779158	Open record: FAD synthetase (CaFADS), riboflavin kinase (RFK) module, riboflavin
riboflavin kinase/FMN hydrolase (AtFMN/FHy)	Arabidopsis thaliana	adenosine 5'-triphosphate	0.443	0.002	222	pH 7.5, 30 °C	PMID 16183635	Open record: riboflavin kinase/FMN hydrolase (AtFMN/FHy), adenosine 5'-triphosphate
riboflavin kinase/FMN hydrolase (AtFMN/FHy)	Arabidopsis thaliana	riboflavin100 mM potassium phosphate buffer	0.419	1.03 × 10⁻⁵	4.07 × 10⁴	pH 7.5, 30 °C	PMID 16183635	Open record: riboflavin kinase/FMN hydrolase (AtFMN/FHy), riboflavin
riboflavin kinase/FMN hydrolase (AtFMN/FHy)	Arabidopsis thaliana	riboflavin	0.583	1.52 × 10⁻⁵	3.84 × 10⁴	pH 7.5, 30 °C	PMID 16183635	Open record: riboflavin kinase/FMN hydrolase (AtFMN/FHy), riboflavin
riboflavin kinase/FMN hydrolase (AtFMN/FHy)	Arabidopsis thaliana	riboflavin 5'-phosphate (flavin mononucleotide)	1.76	6.74 × 10⁻⁴	2.61 × 10³	pH 7.5, 30 °C	PMID 16183635	Open record: riboflavin kinase/FMN hydrolase (AtFMN/FHy), riboflavin 5'-phosphate (flavin mononucleotide)